|Reference : A DESIGN SPACE APPROACH TO DEVELOP A GENERIC CE METHOD FOR THE SEPARATION OF 19 ANTIMALA...|
|Scientific congresses and symposiums : Poster|
|Human health sciences : Pharmacy, pharmacology & toxicology|
|A DESIGN SPACE APPROACH TO DEVELOP A GENERIC CE METHOD FOR THE SEPARATION OF 19 ANTIMALARIAL DRUGS|
|Lamalle, Caroline [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]|
|Marini Djang'Eing'A, Roland [Université de Liège - ULg > Département de pharmacie > Chimie analytique >]|
|Debrus, Benjamin [Université de Liège - ULg > Département de pharmacie > Chimie analytique >]|
|Servais, Anne-Catherine [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]|
|Crommen, Jacques [Université de Liège - ULg > Département de pharmacie > Département de pharmacie >]|
|Hubert, Philippe [Université de Liège - ULg > Département de pharmacie > Chimie analytique >]|
|Fillet, Marianne [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]|
|International Symposium on Drug Analysis|
|21-24 septembre 2010|
|[en] This project consists in analysing different molecules used against malaria by capillary electrophoresis (CE). As qualitative and quantitative counterfeit is largely present in Africa, it is important to develop a simple method which can control the conformity of medicines from African market. For the moment no CE method has been developed to analyse simultaneously the most common antipaludics; but it can be very useful for the control of unknown tablets.
The method development was performed on 4 preservatives (methylparaben, propylparaben, butylhydroxyanisol and butylhydroxytoluen) and 16 antipaludics (artesunate, artemether, amodiaquine hydrochloride, chloroquine diphosphate, piperaquine, primaquine diphosphate, quinine hydrochloride, cinchonine, mefloquine hydrochloride, lumefantrine, halofantrine, sulfadoxine, sulfalen, atovaquone, proguanil hydrochloride, pyrimethamine). Micellar electrokinetic chromatography (MEKC) was chosen because of the presence of neutral and charged compounds in the studied mixture.
The first step of method development was to screen CE experimental conditions to select the most crucial factors. Several conditions were tested with antipaludics diluted in 100% methanol and in 70:30 (v/v) methanol/water in which resolution was better. Four parameters as well as their investigation domain were then chosen: pH (5-10), SDS concentration (20-90nM), acetonitrile proportion (10-40%) and temperature (20-35°C). Then, in order to predict the best condition for the method, an experimental design methodology using a face-centered central composite design (CCD) was realised. Twenty five experiments were defined by CCD. Molecules were separated in four groups and each molecule could be found in two groups. Four samples containing 10 molecules were therefore injected to reduce the number of runs. All the results were analysed and allowed the prediction of optimal conditions in terms of analyte separation. Finally, the condition giving the best separation was tested to verify the prediction.
|Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS; Projet PPP Région Wallonne|
|Researchers ; Professionals ; Students ; General public|
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