Reference : Electrospray Mass Spectrometry of Telomeric RNA (TERRA) Reveals the Formation of Stable ...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Physical, chemical, mathematical & earth Sciences : Chemistry
http://hdl.handle.net/2268/66137
Electrospray Mass Spectrometry of Telomeric RNA (TERRA) Reveals the Formation of Stable Multimeric G-Quadruplex Structures
English
Collie, Gavin W. [CRUK Biomolecular Structure Group, The School of Pharmacy, UniVersity of London, WC1N 1AX London, United Kingdom > > > >]
Parkinson, Gary N. [CRUK Biomolecular Structure Group, The School of Pharmacy, UniVersity of London, WC1N 1AX London, United Kingdom > > > >]
Neidle, Stephen [CRUK Biomolecular Structure Group, The School of Pharmacy, UniVersity of London, WC1N 1AX London, United Kingdom > > > >]
Rosu, Frédéric [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
De Pauw, Edwin mailto [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
Gabelica, Valérie mailto [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
2010
Journal of the American Chemical Society
American Chemical Society
132
27
9328–9334
Yes
International
0002-7863
1520-5126
Washington
DC
[en] mass spectrometry ; noncovalent ; G-quadruplex ; DNA ; RNA ; spectroscopy
[en] We report on the self-assembled structures formed by 12-mer, 22-mer, and 45-mer telomeric RNA (telRNA/TERRA) sequences compared to their DNA analogues, as studied by electrospray mass spectrometry, circular dichroism, and thermal denaturation. The major difference between telomeric RNA and DNA sequences is the ability of telomeric RNA to form higher-order dimeric assemblies, initiated by cation-mediated stacking of two parallel G-quadruplex subunits. The 5′-5′ stacking had been observed recently by NMR for the r(GGGUUAGGGU) 10-mer (Martadinata, H.; Phan, A. T. J. Am. Chem. Soc. 2009, 131, 2570); the present work shows that stacking also occurs for the 22-mer containing four G-tracts and for the 45-mer containing eight G-tracts, suggesting a general structural feature of telomeric RNA. The importance of kinetic effects in multimer formation, unfolding, and structural rearrangements is also highlighted.
Giga-Systems Biology and Chemical Biology
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; EU COST action MP0802
Researchers ; Students
http://hdl.handle.net/2268/66137
10.1021/ja100345z

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