Reference : Innovative development and validation of an HPLC/DAD method for the qualitative and quan...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/4442
Innovative development and validation of an HPLC/DAD method for the qualitative and quantitative determination of major cannabinoids in cannabis plant material
English
De Backer, Benjamin mailto [Université de Liège - ULg > Département de pharmacie > Chimie toxicologique >]
Debrus, Benjamin mailto [Université de Liège - ULg > Département de pharmacie > Chimie analytique >]
Lebrun, Pierre mailto [Université de Liège - ULg > Département de pharmacie > Chimie analytique >]
Theunis, Laetitia mailto [Université de Liège - ULg > Département de pharmacie > Chimie toxicologique >]
Dubois, Nathalie mailto [Université de Liège - ULg > > Toxicologie clinique >]
Decock, Lies [> >]
Verstraete, Alain [> >]
Hubert, Philippe mailto [Université de Liège - ULg > Département de pharmacie > Chimie analytique >]
Charlier, Corinne mailto [Université de Liège - ULg > Département de pharmacie > Chimie toxicologique >]
8-Dec-2009
Journal of Chromatography. B : Analytical Technologies in the Biomedical & Life Sciences
Elsevier Science
877
32
4115-4124
Yes (verified by ORBi)
International
1570-0232
Amsterdam
The Netherlands
[en] HPLC ; Cannabis sativa ; cannabinoids ; THC ; CBD ; CBG
[en] GC is commonly used for the analysis of cannabis samples, e.g. in forensic chemistry. However, as this method is based on heating of the sample, acidic forms of cannabinoids are decarboxylated into their neutral counterparts. Conversely, HPLC permits the determination of the original composition of plant cannabinoids by direct analysis. Several HPLC methods have been described in the literature, but most of them failed to separate efficiently all the cannabinoids or were not validated according to general guidelines.
By use of an innovative methodology for modelling chromatographic responses, a simple and accurate HPLC/DAD method was developed for the quantification of major neutral and acidic cannabinoids present in cannabis plant material: Δ9-tetrahydrocannabinol (THC), THC-acid (THCA), cannabidiol (CBD), CBD-acid (CBDA), cannabigerol (CBG), CBG-acid (CBGA) and cannabinol (CBN). Δ8-tetrahydrocannabinol (∆8-THC) was determined qualitatively. Following the practice of design of experiments, predictive multilinear models were developed and used in order to find optimal chromatographic analytical conditions. The method was validated following an approach using accuracy profiles based on β-expectation tolerance intervals for the total error measurement, and assessing the measurements uncertainty. This analytical method can be used for diverse applications, e.g. plant phenotype determination, evaluation of psychoactive potency and control of material quality.
CIRM
Belspo
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/4442
10.1016/j.jchromb.2009.11.004

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