Cell Line; Cell Nucleus/chemistry; Gene Expression Regulation; Herpesvirus 3, Human/immunology; Humans; Intercellular Adhesion Molecule-1/biosynthesis/genetics; NF-kappa B/metabolism; NF-kappa B p50 Subunit/analysis; NF-kappa B p52 Subunit/analysis; Promoter Regions (Genetics); Protein Binding; RNA, Messenger/biosynthesis; Transcription Factor RelA/metabolism; Tumor Necrosis Factor-alpha/immunology
Abstract :
[en] Intercellular adhesion molecule 1 (ICAM-1) expression is down-regulated in the center of cutaneous varicella lesions despite the expression of proinflammatory cytokines such as gamma interferon and tumor necrosis factor alpha (TNF-alpha). To study the molecular basis of this down-regulation, the ICAM-1 induction of TNF-alpha was analyzed in varicella-zoster virus (VZV)-infected melanoma cells (MeWo), leading to the following observations: (i) VZV inhibits the stimulation of icam-1 mRNA synthesis; (ii) despite VZV-induced nuclear translocation of p65, p52, and c-Rel, p50 does not translocate in response to TNF-alpha; (iii) the nuclear p65 present in VZV-infected cells is no longer associated with p50 and is unable to bind the proximal NF-kappaB site of the icam-1 promoter, despite an increased acetylation and accessibility of the promoter in response to TNF-alpha; and (iv) VZV induces the nuclear accumulation of the NF-kappaB inhibitor p100. VZV also inhibits icam-1 stimulation of TNF-alpha by strongly reducing NF-kappaB nuclear translocation in MRC5 fibroblasts. Taken together, these data show that VZV interferes with several aspects of the immune response by inhibiting NF-kappaB binding and the expression of target genes. Targeting NF-kappaB activation, which plays a central role in innate and adaptive immune responses, leads to obvious advantages for the virus, particularly in melanocytes, which are a site of viral replication in the skin.
Research center :
Giga-Signal Transduction - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
El Mjiyad, Nadia ; Université de Liège - ULiège > Département des sciences de la vie > Virologie - Immunologie
Piette, Jacques ; Université de Liège - ULiège > Département des sciences de la vie > Virologie - Immunologie - GIGA-Research
Sadzot-Delvaux, Catherine ; Université de Liège - ULiège > Département des sciences de la vie > Virologie et immunologie - GIGA-M : Coordination scientifique
Language :
English
Title :
Varicella-zoster virus modulates NF-kappaB recruitment on selected cellular promoters.
Publication date :
2007
Journal title :
Journal of Virology
ISSN :
0022-538X
eISSN :
1098-5514
Publisher :
American Society for Microbiology (ASM), Washington, United States - District of Columbia
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