Reference : EGF stimulates Pit-1 independent transcription of the human prolactin pituitary promo...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/34302
EGF stimulates Pit-1 independent transcription of the human prolactin pituitary promoter in human breast cancer SK-BR-3 cells through its proximal AP-1 response element
English
Manfroid, Isabelle mailto [Université de Liège - ULg > > GIGA-Research >]
Van de Weerdt, Cécile mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Baudhuin, Ariane [> > > >]
Martial, Joseph mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Muller, Marc mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
2005
Molecular & Cellular Endocrinology
North Holland Publishing Company
229
1-2
127-39
Yes (verified by ORBi)
International
0303-7207
Amsterdam
The Netherlands
[en] 1-Phosphatidylinositol 3-Kinase/metabolism ; Breast Neoplasms/genetics/*metabolism ; DNA-Binding Proteins/*deficiency/genetics ; Epidermal Growth Factor/*pharmacology ; Humans ; JNK Mitogen-Activated Protein Kinases/metabolism ; MAP Kinase Kinase 4 ; Mitogen-Activated Protein Kinase Kinases/metabolism ; Pituitary Gland/*physiology ; Prolactin/*genetics/metabolism ; Promoter Regions, Genetic/*genetics ; Proto-Oncogene Proteins c-fos/metabolism ; Proto-Oncogene Proteins c-jun/metabolism ; RNA, Messenger/genetics/metabolism ; Receptor, erbB-2/metabolism ; Regulatory Sequences, Nucleic Acid/physiology ; Signal Transduction ; Transcription Factor AP-1/genetics/metabolism ; Transcription Factor Pit-1 ; Transcription Factors/*deficiency/genetics ; *Transcription, Genetic ; Transcriptional Activation ; Tumor Cells, Cultured
[en] Normal and neoplastic human mammary gland cells are targets for the proliferative action of prolactin. These cells also synthesize prolactin, thereby inducing an autocrine/paracrine proliferative loop. We present the first extensive analysis of the transcriptional regulation of the human prolactin gene (hPRL) in human mammary tumor cells, SK-BR-3. We show that the pituitary promoter is functional in these cells in the absence of the pituitary-specific factor Pit-1. Expression of exogenous Pit-1 or epidermal growth factor (EGF) treatment stimulates the transfected hPRL pituitary promoter and the endogenous hPRL expression. EGF stimulation is mediated by increased synthesis of c-fos and c-jun, resulting in AP-1 binding to the proximal hPRL pituitary promoter. This regulation involves the EGF receptor, possibly ErbB2 that is highly expressed in SK-BR-3 cells, and a PI3K/JNK pathway. The stimulation of hPRL gene transcription by EGF in mammary cells may include hPRL in a complex regulatory network controlling growth of human mammary cells.
http://hdl.handle.net/2268/34302
10.1016/j.mce.2004.08.009
2004/12/21

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