ORBi: Manfroid Isabelle - EGF stimulates Pit-1 independent transcription of the human prolactin pituitary promoter in human breast cancer SK-BR-3 cells through its proximal AP-1 response element

Reference : EGF stimulates Pit-1 independent transcription of the human prolactin pituitary promoter...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/34302

Title :	EGF stimulates Pit-1 independent transcription of the human prolactin pituitary promoter in human breast cancer SK-BR-3 cells through its proximal AP-1 response element
Language :	English
Author, co-author :	Manfroid, Isabelle [Université de Liège - ULg > > GIGA-Research >]
	Van de Weerdt, Cécile [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
	Baudhuin, Ariane [> > > >]
	Martial, Joseph [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
	Muller, Marc [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Publication date :	2005
Journal title :	Molecular & Cellular Endocrinology
Publisher :	North Holland Publishing Company
Volume :	229
Issue/season :	1-2
Pages :	127-39
Audience :	International
ISSN :	0303-7207
City :	Amsterdam
Country :	The Netherlands
Keywords :	[en] 1-Phosphatidylinositol 3-Kinase/metabolism ; Breast Neoplasms/genetics/metabolism ; DNA-Binding Proteins/deficiency/genetics ; Epidermal Growth Factor/pharmacology ; Humans ; JNK Mitogen-Activated Protein Kinases/metabolism ; MAP Kinase Kinase 4 ; Mitogen-Activated Protein Kinase Kinases/metabolism ; Pituitary Gland/physiology ; Prolactin/genetics/metabolism ; Promoter Regions, Genetic/genetics ; Proto-Oncogene Proteins c-fos/metabolism ; Proto-Oncogene Proteins c-jun/metabolism ; RNA, Messenger/genetics/metabolism ; Receptor, erbB-2/metabolism ; Regulatory Sequences, Nucleic Acid/physiology ; Signal Transduction ; Transcription Factor AP-1/genetics/metabolism ; Transcription Factor Pit-1 ; Transcription Factors/deficiency/genetics ; Transcription, Genetic ; Transcriptional Activation ; Tumor Cells, Cultured
Abstract :	[en] Normal and neoplastic human mammary gland cells are targets for the proliferative action of prolactin. These cells also synthesize prolactin, thereby inducing an autocrine/paracrine proliferative loop. We present the first extensive analysis of the transcriptional regulation of the human prolactin gene (hPRL) in human mammary tumor cells, SK-BR-3. We show that the pituitary promoter is functional in these cells in the absence of the pituitary-specific factor Pit-1. Expression of exogenous Pit-1 or epidermal growth factor (EGF) treatment stimulates the transfected hPRL pituitary promoter and the endogenous hPRL expression. EGF stimulation is mediated by increased synthesis of c-fos and c-jun, resulting in AP-1 binding to the proximal hPRL pituitary promoter. This regulation involves the EGF receptor, possibly ErbB2 that is highly expressed in SK-BR-3 cells, and a PI3K/JNK pathway. The stimulation of hPRL gene transcription by EGF in mammary cells may include hPRL in a complex regulatory network controlling growth of human mammary cells.
Permalink :	http://hdl.handle.net/2268/34302
DOI :	10.1016/j.mce.2004.08.009
Mentions required by the publisher for OA :	2004/12/21

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