Reference : Zebrafish bone and general physiology are differently affected by hormones or changes...
Scientific journals : Article
Life sciences : Genetics & genetic processes
http://hdl.handle.net/2268/180826
Zebrafish bone and general physiology are differently affected by hormones or changes in gravity.
English
Aceto, Jessica mailto [Université de Liège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Nourizadeh-Lillabadi, Rasoul [> >]
Marée, Raphaël mailto [Université de Liège > > GIGA-Research >]
Dardenne, Nadia mailto [Université de Liège > Département des sciences de la santé publique > Santé publique, Epidémiologie et Economie de la santé >]
Jeanray, Nathalie mailto [Université de Liège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Wehenkel, Louis mailto [Université de Liège > Dép. d'électric., électron. et informat. (Inst.Montefiore) > Systèmes et modélisation >]
Aleström, Peter [> >]
van Loon, Jack [> >]
Muller, Marc mailto [Université de Liège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
2015
PLoS ONE
Public Library of Science
10
6
1-42
Yes (verified by ORBi)
International
1932-6203
San Franscisco
CA
[en] zebrafish ; skeleton ; gravity
[en] Teleost fish such as zebrafish (Danio rerio) are increasingly used for physiological, genetic
and developmental studies. Our understanding of the physiological consequences of altered
gravity in an entire organism is still incomplete. We used altered gravity and drug treatment
experiments to evaluate their effects specifically on bone formation and more generally on
whole genome gene expression. By combining morphometric tools with an objective scoring
system for the state of development for each element in the head skeleton and specific gene
expression analysis, we confirmed and characterized in detail the decrease or increase of bone
formation caused by a 5 day treatment (from 5dpf to 10 dpf) of, respectively parathyroid
hormone (PTH) or vitamin D3 (VitD3). Microarray transcriptome analysis after 24 hours
treatment reveals a general effect on physiology upon VitD3 treatment, while PTH causes
more specifically developmental effects. Hypergravity (3g from 5dpf to 9 dpf) exposure
results in a significantly larger head and a significant increase in bone formation for a subset
of the cranial bones. Gene expression analysis after 24 hrs at 3g revealed differential
expression of genes involved in the development and function of the skeletal, muscular,
nervous, endocrine and cardiovascular systems. Finally, we propose a novel type of
experimental approach, the "Reduced Gravity Paradigm", by keeping the developing larvae at
3g hypergravity for the first 5 days before returning them to 1g for one additional day. 5 days
exposure to 3g during these early stages also caused increased bone formation, while gene
expression analysis revealed a central network of regulatory genes (hes5, sox10, lgals3bp,
egr1, edn1, fos, fosb, klf2, gadd45ba and socs3a) whose expression was consistently affected
by the transition from hyper- to normal gravity.
Giga-Development and Stem Cells ; Applied and Fundamental FISH Research Center - AFFISH-RC ; Centre Interfacultaire d'Analyse des Résidus en Traces - CART
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/180826
also: http://hdl.handle.net/2268/183119
10.1371/journal.pone.0126928
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0126928

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