Article (Scientific journals)
Zebrafish bone and general physiology are differently affected by hormones or changes in gravity.
Aceto, Jessica; Nourizadeh-Lillabadi, Rasoul; Marée, Raphaël et al.
2015In PLoS ONE, 10 (6), p. 1-42
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Keywords :
zebrafish; skeleton; gravity
Abstract :
[en] Teleost fish such as zebrafish (Danio rerio) are increasingly used for physiological, genetic and developmental studies. Our understanding of the physiological consequences of altered gravity in an entire organism is still incomplete. We used altered gravity and drug treatment experiments to evaluate their effects specifically on bone formation and more generally on whole genome gene expression. By combining morphometric tools with an objective scoring system for the state of development for each element in the head skeleton and specific gene expression analysis, we confirmed and characterized in detail the decrease or increase of bone formation caused by a 5 day treatment (from 5dpf to 10 dpf) of, respectively parathyroid hormone (PTH) or vitamin D3 (VitD3). Microarray transcriptome analysis after 24 hours treatment reveals a general effect on physiology upon VitD3 treatment, while PTH causes more specifically developmental effects. Hypergravity (3g from 5dpf to 9 dpf) exposure results in a significantly larger head and a significant increase in bone formation for a subset of the cranial bones. Gene expression analysis after 24 hrs at 3g revealed differential expression of genes involved in the development and function of the skeletal, muscular, nervous, endocrine and cardiovascular systems. Finally, we propose a novel type of experimental approach, the "Reduced Gravity Paradigm", by keeping the developing larvae at 3g hypergravity for the first 5 days before returning them to 1g for one additional day. 5 days exposure to 3g during these early stages also caused increased bone formation, while gene expression analysis revealed a central network of regulatory genes (hes5, sox10, lgals3bp, egr1, edn1, fos, fosb, klf2, gadd45ba and socs3a) whose expression was consistently affected by the transition from hyper- to normal gravity.
Research center :
Giga-Development and Stem Cells - ULiège
AFFISH-RC - Applied and Fundamental FISH Research Center - ULiège
CART - Centre Interfacultaire d'Analyse des Résidus en Traces - ULiège
Disciplines :
Genetics & genetic processes
Author, co-author :
Aceto, Jessica ;  Université de Liège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Nourizadeh-Lillabadi, Rasoul
Marée, Raphaël  ;  Université de Liège > GIGA-Research
Dardenne, Nadia  ;  Université de Liège > Département des sciences de la santé publique > Santé publique, Epidémiologie et Economie de la santé
Jeanray, Nathalie ;  Université de Liège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Wehenkel, Louis  ;  Université de Liège > Dép. d'électric., électron. et informat. (Inst.Montefiore) > Systèmes et modélisation
Aleström, Peter
van Loon, Jack
Muller, Marc  ;  Université de Liège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Language :
English
Title :
Zebrafish bone and general physiology are differently affected by hormones or changes in gravity.
Publication date :
2015
Journal title :
PLoS ONE
eISSN :
1932-6203
Publisher :
Public Library of Science, San Franscisco, United States - California
Volume :
10
Issue :
6
Pages :
1-42
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 23 April 2015

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