Article (Scientific journals)
Validation of a method for simultaneous isolation of shiga toxin-producing Escherichia coli O26, O103, O111, and O145 from minced beef by an international ring-trial
Verstraete, K.; De Zutter, L.; Robyn, J. et al.
2012In Foodborne Pathogens and Disease, 9 (5), p. 412-417
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Keywords :
Shiga toxin producing Escherichia coli; Escherichia coli
Abstract :
[en] An isolation method described by Possé et al. (FEMS Microbiol Lett 2008;282:124-131) was satisfactorily validated in an international ring-trial using artificially contaminated minced beef samples. Until now, no validated method existed for the simultaneous isolation of Shiga toxin-producing Escherichia coli serogroups O26, O103, O111, and O145 in food. Twelve laboratories from five European countries participated and received 16 inoculated beef samples contaminated with cold-stressed cells of the four serogroups O26, O103, O111, and O145 in two levels (approximately 30 and 300 CFU 25g-1) in duplicate. In addition, they received four non-inoculated samples. The isolation protocol comprised a selective enrichment step, a selective isolation step on a non-O157 agar plate differentiating the serogroups by color, followed by confirmation by plating on confirmation agar media and agglutination. All laboratories were able to isolate the inoculated serogroups from the samples, both for the high and the low inoculation level. Results did not differ whether in-house-prepared or ready-to-use non-O157 agar plates were used, demonstrating that by following the instructions laboratories managed to perform the complete protocol with success. © Copyright 2012, Mary Ann Liebert, Inc.
Disciplines :
Microbiology
Author, co-author :
Verstraete, K.;  Technology and Food Science Unit, Institute for Agricultural and Fisheries Research, Brusselsesteenweg 370, 9090 Melle, Belgium
De Zutter, L.;  Department of Veterinary Health and Food Safety, Ghent University, Merelbeke, Belgium
Robyn, J.;  Technology and Food Science Unit, Institute for Agricultural and Fisheries Research, Brusselsesteenweg 370, 9090 Melle, Belgium
Daube, Georges  ;  Université de Liège - ULiège > Département de sciences des denrées alimentaires > Microbiologie des denrées alimentaires
Herman, L.;  Technology and Food Science Unit, Institute for Agricultural and Fisheries Research, Brusselsesteenweg 370, 9090 Melle, Belgium
Heyndrickx, M.;  Technology and Food Science Unit, Institute for Agricultural and Fisheries Research, Brusselsesteenweg 370, 9090 Melle, Belgium, Department of Pathology, Bacteriology and Poultry Diseases, Ghent University, Merelbeke, Belgium
De Schaetzen, M.-A.;  Food Science Department, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium
De Reu, K.;  Technology and Food Science Unit, Institute for Agricultural and Fisheries Research, Brusselsesteenweg 370, 9090 Melle, Belgium
Language :
English
Title :
Validation of a method for simultaneous isolation of shiga toxin-producing Escherichia coli O26, O103, O111, and O145 from minced beef by an international ring-trial
Publication date :
2012
Journal title :
Foodborne Pathogens and Disease
ISSN :
1535-3141
eISSN :
1556-7125
Publisher :
Mary Ann Liebert, United States
Volume :
9
Issue :
5
Pages :
412-417
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 01 February 2013

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