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See detailEYK1 encoding erythrulose kinase as a catabolic selectable marker for genome editing in the non-conventional yeast Yarrowia lipolytica
Vandermies, Marie ULiege; Denies, Olivia ULiege; Nicaud, Jean-Marc et al

in Journal of Microbiological Methods (2017), 139

We report here on EYK1, encoding erythrulose kinase, as an efficient catabolic selectable marker for genome editing in Y. lipolytica. Compared to auxotrophic markers, EYK1 increases the growth rate of ... [more ▼]

We report here on EYK1, encoding erythrulose kinase, as an efficient catabolic selectable marker for genome editing in Y. lipolytica. Compared to auxotrophic markers, EYK1 increases the growth rate of transformants and allows improved efficiency of transformation. The utility of the marker EYK1 in a replicative vector was also demonstrated. [less ▲]

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See detailHighly efficient doped nanocristalline TiO2 for water Treatment
Mahy, Julien ULiege; Léonard, Géraldine ULiege; Zubiaur, Anthony ULiege et al

Conference (2017, July 13)

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See detailDu dessin au design. Seth comme auteur-rééditeur
Crucifix, Benoît ULiege

Conference (2017, July 13)

Depuis ces dernières années, l’édition de bande dessinée connaît un véritable boom de rééditions patrimoniales. Dans le contexte nord-américain, ce sont justement les auteurs associés au « roman ... [more ▼]

Depuis ces dernières années, l’édition de bande dessinée connaît un véritable boom de rééditions patrimoniales. Dans le contexte nord-américain, ce sont justement les auteurs associés au « roman graphique » – ceux-là même qui ont exploré le livre en tant qu’objet en réalisant eux-mêmes le design de leurs œuvres – qui sont aussi invités à prendre en charge la conception graphique de ces rééditions d’œuvres du passé qu’ils aident à faire (re)découvrir. Cette communication a pour but d’étudier les designs réalisés par l’auteur canadien Seth pour plusieurs œuvres complètes : The Complete Peanuts, The John Stanley Library et The Collected Doug Wright. Les designs de Seth se caractérisent par leur mise-en-avant d’une esthétique idiosyncratique, comme il l’a lui-même exprimé : « when I design something it really is too much about me » (Spurgeon, 143). Elles se lient donc intimement à la production « propre » de l’auteur. Dans le cas de Stanley et Doug Wright, ces rééditions sont d’ailleurs liées à des essais écrits par Seth, relevant d’un travail autant esthétique qu’historiographique. Il s’agira d’abord de comprendre la spécificité culturelle de cette pratique de réédition dans l’édition « alternative » américaine, qui diffère du contexte éditorial européen ; plus en avant, le cas de Seth permettra de comprendre comment cette pratique ressort d’un mécanisme de canonisation particulier (Heer), mais aussi de montrer à quel point ce type d’objets met en avant une « fonction-auteur » (Foucault) inextricablement liée à une activité historiographique, éditoriale et patrimoniale. [less ▲]

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See detailPanel “Quand la bande dessinée se reproduit : imitation, reprise et rétro-réflexivité”
Crucifix, Benoît ULiege; Busi-Rizzi, Giorgio

Diverse speeche and writing (2017)

Co-organisation et présidence du panel "Quand la bande dessinée se reproduit : imitation, reprise et rétro-réflexivité" – Dans une industrie culturelle basée sur les principes de série et d’imitation, les ... [more ▼]

Co-organisation et présidence du panel "Quand la bande dessinée se reproduit : imitation, reprise et rétro-réflexivité" – Dans une industrie culturelle basée sur les principes de série et d’imitation, les questions de reproduction occupent une place toute particulière, illustrée par ce terme spécifique de swipe utilisé dans l’industrie du comic book. L’imitation s’est instituée comme principe éditorial autant dans la bande dessinée nord-américaine que dans la bande dessinée franco-belge : le style graphique se constitue ainsi souvent par imitation et reproduction, comme le montre l’exemple de l’émulation des styles ‘singuliers’ d’artistes tels que Jack Kirby ou André Franquin. Les rééditions des œuvres du passé tout comme les reprises de série classique s’épanouissent aujourd’hui et, simultanément, la bande dessinée s’empare de plus en plus de son propre passé, invoquant les connaissances encyclopédiques de ses lecteurs et s’engageant dans une forme de « rétro-réflexivité » (Brett Camper). Ces pratiques de reproduction par imitation indiquent différentes manières de penser la mise-à-disposition du passé dans le présent. Elles négocient ainsi de façon spécifique et historique le rapport à la reproductibilité en bande dessinée, par le biais de diverses techniques (recopiage, décalquage, collage, dessin, détournement, réinterprétation graphique, photocopie, clins d’œil, copier/coller, etc.) : à chaque fois, elles semblent alors interroger le rapport entre dessin et reproduction, que celle-ci soit manuelle, mécanique ou numérique. Cette session portera sur la prégnance de la reproduction par imitation dans la bande dessinée afin d’en examiner les enjeux culturels et esthétiques qui la lient à son passé et à son histoire. Comment la bande dessinée se reproduit-elle elle-même ? Quelles sont les pratiques de citation qui lui sont propres ? Comment ce rapport au passé a-t-il changé ? [less ▲]

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See detailEffect of surface modification of polylactides on fibroblasts L-929 and osteoblasts MG-63
Tilkin, Rémi ULiege; Regibeau, Nicolas ULiege; Lambert, Stéphanie ULiege et al

Conference (2017, July 12)

During the past few years, tissue engineering has become one of the most promising techniques to maintain, improve, or reconstruct human tissue, even complete human organs. This solution is frequently ... [more ▼]

During the past few years, tissue engineering has become one of the most promising techniques to maintain, improve, or reconstruct human tissue, even complete human organs. This solution is frequently based on the realization of temporary porous matrices, also called "scaffolds", design as model and structure for the development of cells. Surface properties of those biomaterials are some determinant criteria for cell adhesion and proliferation. The scope of this work is the study of adhesion and proliferation of fibroblasts L-929 and osteoblasts MG-63 on untreated, hydrolyzed or aminolyzed polylactides. First, these substrates are developed: poly-L,D-lactide (PDLLA) powder is pressed into disks. The surface of disks was chemically modified by hydrolysis and aminolysis surface treatments. Surface modifications were based on previous studies. [1,2] The hydrolysis process is carried out by immersing PDLLA disks in 2 M NaOH for 20 min at room temperature (24°C) under constant agitation. The aminolysis process is performed by placing PDLLA disks in 15 % ethylenediamine in isopropanol for 5 min at room temperature (24°C) under constant agitation. Substrates are characterized by optical microscopy, Scanning Electron Microscopy and water contact angle. Finally, cell adhesion, proliferation, and viability on these substrates are assessed by cell counting and MTT assay after 1, 4, and 8 days of cell culture. Results from surface characterization show an increase of roughness for hydrolyzed polylactide. Regarding water contact angle measurements, values are smaller on treated substrates even though the difference with untreated substrates appears smaller than expected in the literature. [1,2] Cell counting and MTT assay show an increase of cell proliferation and cell viability for treated polylactide substrates in the case of fibroblasts and a decrease of cell proliferation for aminolyzed polylactide substrates in the case of osteoblasts. In conclusions, this work highlights the different effects of surface modification on fibroblasts and osteoblasts viability. References : [1]. Wang YQ, Cai JY. Enhanced cell affinity of poly(L-lactic acid) modified by base hydrolysis: wettability and surface roughness at nanometer scale. Current Applied Physics 2007, 7(S1):e108–e111. [2]. Lin Y, Chrzanowski W, Knowles J, Bishop A, Bismarck A. Functionalized poly(D,L-lactide) for pulmonary epithelial cell culture. Advanced Engineering Materials 2010, 12(4): B101–B112. [less ▲]

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See detailLANDSLIDE SUSCEPTIBILITY PREDICTION IN A MOUNTAINOUS CATCHMENT: THE NARANJO BASIN, WESTERN GUATEMALA
Estrada Orozco, Nick Kenner ULiege

Doctoral thesis (2017)

In tropical mountain areas, landslides triggered by heavy rainfall represent a natural hazard, especially where meteorological events such as tropical cyclones are recurrent. Rainfall-triggered landslides ... [more ▼]

In tropical mountain areas, landslides triggered by heavy rainfall represent a natural hazard, especially where meteorological events such as tropical cyclones are recurrent. Rainfall-triggered landslides may also increase in highly seismic active areas. This is the case of Guatemala, located in the Circum-Pacific Belt and between Pacific and Atlantic Oceans, sources of cyclonic events. Although Guatemala is a region where landslides are a widespread phenomenon, landslide inventories, maps and quantitative susceptibility assessments that are useful for land use planners and decision makers are hitherto non-existent. The aim of this research is to produce quantitative landslide susceptibility assessments, using logistic regression multivariate statistical method. The study area is a watershed located in the department of San Marcos (western Guatemala), impacted by a tropical storm event in October 2005. A total of 766 landslides were identified and mapped using orthoimages from 2005. Then, 99 landslides were mapped in 2011 based on field data. The main landslide type is shallow landslide (61 % in 2005), while 39 % of those landslides from 2005 evolved into debris flows. In total, susceptibility models using multivariate probabilistic approach were developed for shallow landslides, evaluating two different strategies for the sample size of non-landslides events and three different numbers of input variable in the models. Susceptibility models were developed for debris flows dataset and the union of both dataset (shallow and debris flows). The comparison of the models and the associated susceptibility maps highlighted 6 significant input variables that are associated with landslide occurences - elevation, slope, aspect, profile curvature, planform curvature and distance to roads. Performance comparisons of models were also carried out. To validate the performance of the model results, the ROC curve was used, as well as the four-fold and confusion matrix plots. A susceptibility map was generated to display the results of the models in terms of probability values. A proposal and discussion on the operational use of susceptibility maps where cutoff values can be chosen to define the lowest and highest landslide susceptibility were also made. These will help land use planners in decision-making and in implementing protective measures. [less ▲]

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See detailRECONSTRUCTION DE L'ÉVOLUTION DU RAYONNEMENT SOLAIRE REÇU EN SURFACE EN EUROPE OCCIDENTALE SUR LA PÉRIODE 1900-2014 À L'AIDE DU MODÈLE ATMOSPHÉRIQUE RÉGIONAL MAR
Wyard, Coraline ULiege; Fettweis, Xavier ULiege; Belleflamme, Alexandre ULiege et al

in Dahech, Salem; Charfi, Sami (Eds.) Actes du XXXe colloque de l'Association Internationale de Climatologie : Climat, ville et environnement (2017, July)

Many studies show that the surface solar radiation has underwent large variations over the second half of the 20th century as a result of variations in cloud cover and aerosol loading in the atmosphere ... [more ▼]

Many studies show that the surface solar radiation has underwent large variations over the second half of the 20th century as a result of variations in cloud cover and aerosol loading in the atmosphere. However, it is difficult to build strong conclusions before the 1950' because of the observations scarcity. The evolution of the surface solar radiation has been reconstructed over 1900-2014 using the regional model MAR (« Modèle Atmosphérique Régional ») in Belgium. Boundary conditions were provided by four reanalysis products : the ERA-interim (1979-2014) completed by the ERA40 (1958-1978), the NCEP/NCAR-v1 (1948-2014), the ERA-20C (1900-2010) and the 20CRV2C (1900-2010). Results show that the reanalyses can generate divergent trends while they assimilate observations and are supposed to represent the same climate. [less ▲]

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See detailStandardized Settling Cell Design
Leleu, David ULiege; Pfennig, Andreas ULiege

Scientific conference (2017, July)

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See detailÉvolution de l'enneigement moyen dans les Alpes au cours du 20e siècle à l'aide du modèle atmosphérique régional MAR
Belleflamme, Alexandre ULiege; Wyard, Coraline ULiege; Doutreloup, Sébastien ULiege et al

in Dahech, Salem; Charfi, Sami (Eds.) Actes du XXXème colloque de l'Association Internationale de Climatologie - Climat, ville et environnement (2017, July)

The evolution of the snow height over the Alps can strongly impact tourism, but also the water availability of the region. In this study, we have reproduced the evolution of the climate in the Alps over ... [more ▼]

The evolution of the snow height over the Alps can strongly impact tourism, but also the water availability of the region. In this study, we have reproduced the evolution of the climate in the Alps over the 20th century with the help of the regional atmospheric model MAR forced by three reanalyses (ERA-20C, NCEP/NCAR, and ERA-Interim). MAR shows that the snow height has increased since the beginning of the 20th century, first only at higher altitudes, then also at lower levels, before knowing a strong and abrupt decrease between 1985 and 1990. This evolution, which is consistent with observations given in the literature, is directly linked with the trends of NAO and AO. In fact, the atmospheric circulation changes highlighted by NAO and AO induce temperature and precipitation changes that directly determine the snow height in the Alps. [less ▲]

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See detailEFFECT OF FENTANYL INFUSION ON MINIMAL ALVEOLAR CONCENTRATION IN ISOFLURANE ANAESTHETISED HORSES – PRELIMINARY RESULTS
Vanaga, Jana; Kovalcuka, Liga; Sandersen, Charlotte ULiege

Poster (2017, April 25)

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See detail9. Nutrition azotée de l’épeautre en Ardenne et en région limoneuse
Escarnot, Emmanuelle; Meza Morales, Walter ULiege; Crémer, S. et al

in Bodson, Bernard; Watillon, Bernard (Eds.) Livre Blanc Céréales (2017, February 22)

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See detail2. Implantation des cultures
Eylenbosch, Damien ULiege; Meza Morales, Walter ULiege; Monfort, Bruno et al

in Bodson, Bernard; Watillon, Bernard (Eds.) Livre Blanc Céréales (2017, February 22)

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See detailBehavioural phenotyping of SV2A lox/lox mice: Motor and anxiety-like features
Serrano Navacerrada, Maria Elisa ULiege; Bartholomé, Odile ULiege; Van Den Ackerveken, Priscilla ULiege et al

Poster (2017)

Background: Epilepsy is one of the most common neurological disorders (Alexopoulos, 2004). Current anti-epileptic drugs, such as Levetiracetam (Keppra®) or Brivaracetam, mainly target the trans-membrane ... [more ▼]

Background: Epilepsy is one of the most common neurological disorders (Alexopoulos, 2004). Current anti-epileptic drugs, such as Levetiracetam (Keppra®) or Brivaracetam, mainly target the trans-membrane Synaptic Vesicle Protein 2A (Hamann et al., 2008). Studies on homozygous SV2A KO mice phenotype, prove the mice to suffer severe seizures and die within 3 weeks (Crowder et al., 1999), establishing a link between this protein and the epilepsy. In 2009, the availability of heterozygous SV2A (+/-) mice as research tool enabled shedding light on the role of protein SV2A, revealing no motor differences but anxiety-like features in these mice compared with the WT (Lamberty et al., 2009), and a pro-epileptic phenotype (Crowder et al., 1999; Kaminski et al., 2008). Recently, a floxed SV2A mouse model has been produced with the Cre/loxP recombination system, this model allows invalidating the protein in CA3 hippocampal region, not followed by epileptic seizures (Menten-Dedoyart et al., 2016). Objectives: Perform a first behavioural phenotyping of SV2A lox/lox mice. Methodology: Two experiments were conducted in parallel to evaluate the effect of 3 different genotypes in the phenotype: WT (Grik4-/-, SV2A lox/lox), HZ (Grik4 +/-, SV2A lox/+) and cKO (Grik4 +/-, SV2A lox/lox) in male (n = 42) and female (n = 33) separately . Mice were housed individually along the experiment, with standard food and water ad libitum. After an acclimatization period of 2 weeks, anxiety-like features as well as exploration abilities were evaluated in an elevated plus-maze (EPM) single session of 5 minutes). 3 days later, spontaneous locomotor activity and habituation to the environment were measured during 1 hour, 3 consecutive days, in the activity chambers (ACT). Results: One-way ANOVA in EPM data presented no significant differences between groups, either in males or in females. A significant difference was found, between time spent in close arms vs open arms (p<0.01; η2p = 0.738 males; η2p = 0.805 females). Mixed between-within subjects ANOVA in ACT reflected no significant differences between groups in both sexes, regarding spontaneous locomotor activity and acclimatization to the activity chamber (p>0.05). Statistical significant differences were found between the 3 days (p<0.01; η2p = 0.716 males; η2p = 0.663 females). Conclusion: Results indicate that a decrease in the hippocampal expresion of SV2A protein does not lead to major behavioral changes. Regarding locomotor activity, the results found in heterozygous SV2A (+/-) mice are in line with (Lamberty et al., 2009), however, our mice did not present anxiety-like features, being necessary a global decrease in brain SV2A levels and not only a partial loss in a restricted region of the brain. Further analyses increasing the number of mice per group, will allow us to intensify our power value from 50-60% (females-males) up to 80%, with large effect size and a signification of p<0.05. An additional test to evaluate the spatial memory may help us better understand the effect a specific reduction in SV2A hippocampal expression has on the phenotype of mice. [less ▲]

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See detailInscription territorial et esprit du lieu
Schmitz, Serge ULiege

in Lempereur, Françoise (Ed.) Patrimoine culturel immatériel (2017)

After an introduction to the cultural geographies, the landscape is parsed as the visible face of the organisation of societies, and then the short chapter explores the meaning and the spirit of places.

Detailed reference viewed: 47 (6 ULiège)
See detailAnalyse spatiale et cartographie thématique
Schmitz, Serge ULiege

in Lempereur, Françoise (Ed.) Patrimoine culturel immatériel (2017)

This short chapter encourages the researcher to search the spatial organization of intangible cultural heritage and to make use of maps.

Detailed reference viewed: 28 (9 ULiège)