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See detailTaxonomic Identification of Cyanobacteria by a Polyphasic Approach
Wilmotte, Annick ULg; Laughinghouse, Dail Haywood IV; Capelli, Camilla et al

in Kurmayer, Rainer; Sivonen, Kaarina; Wilmotte, Annick (Eds.) et al Molecular Tools for the Detection and Quantification of Toxigenic Cyanobacteria (2017)

In this chapter, we shall discuss the criteria and methods to be adopted for the taxonomic identification and classification of cyanobacteria. This includes a brief introduction of the two Codes of ... [more ▼]

In this chapter, we shall discuss the criteria and methods to be adopted for the taxonomic identification and classification of cyanobacteria. This includes a brief introduction of the two Codes of Nomenclature ruling in parallel on the valid naming of these organisms. We shall then present the major steps important for cyanobacterial taxa identification and their nomenclature. These include: 1) determination of morphology by light microscopy; 2) genetic characterization by single and/or multilocus sequence typing; 3) the assignment of the organism to a taxonomic entity (genus, species, eco- and/or genotype within a species) by reference to, and phylogenetic analysis of, cyanobacterial nucleotide sequences available in public repositories. Ideally, these methods should be accompanied by the determination of other relevant properties (ultrastructural, physiological, biochemical, and ecological characteristics) that may help to define/redefine and circumscribe the cyanobacterial taxon under study. [less ▲]

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See detailIsolation, Purification, and Cultivation of Toxigenic Cyanobacteria
Haande, Sigrid; Jasser, Iwona; Gugger, Muriel et al

in Kurmayer, Rainer; Sivonen, Kaarina; Wilmotte, Annick (Eds.) et al Molecular Tools for the Detection and Quantification of Toxigenic Cyanobacteria (2017)

This chapter summarizes the most commonly used methods for the isolation, purification, and cultivation of toxic cyanobacteria. The aim is to give general advice on how to isolate and maintain clonal ... [more ▼]

This chapter summarizes the most commonly used methods for the isolation, purification, and cultivation of toxic cyanobacteria. The aim is to give general advice on how to isolate and maintain clonal cyanobacterial cultures in order to use them in genetic studies. The traditional methods for the isolation of cyanobacteria into culture are well established and described and there are several excellent reviews with detailed information on culturing techniques [less ▲]

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See detailDNA (Diagnostic) and cDNA Microarray
Rantala-Yilinen, Anne; Sivonen, Kaarina; Wilmotte, Annick ULg

in Kurmayer, Rainer; Sivonen, Kaarina; Wilmotte, Annick (Eds.) et al Molecular Tools for the Detection and Quantification of Toxigenic Cyanobacteria (2017)

Diagnostic microarrays have been used to study cyanobacterial diversity in environmental samples. They usually include sequences (probes) for only one or a few genes allowing a number of genera/species to ... [more ▼]

Diagnostic microarrays have been used to study cyanobacterial diversity in environmental samples. They usually include sequences (probes) for only one or a few genes allowing a number of genera/species to be detected. In contrast, cDNA microarrays are used for screening genome-wide changes in gene expression and include probes for all or most genes in one strain . The purpose of this sub-chapter is to introduce the principle of a diagnostic microarray (= DNA chip) that uses a ligation detection reaction (LDR) and universal microarray to simultaneously detect and identify all potential microcystin and nodularin producers present in a sample. This is especially useful when monitoring environmental samples that can contain many cyanobacterial genera, including toxin-producing strains. [less ▲]

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See detailAssessing quality of life using structural equation modeling
Dardenne, Nadia ULg; Pétré, Benoît ULg; Husson, Eddy ULg et al

Poster (2017, August)

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See detailEarly eukaryotes: insights from microanalyses of proterozoic microfossils
Cornet, Yohan ULg; Beghin, Jérémie ULg; Kabamba Baludikay, Blaise ULg et al

in Goldschmidt Abstracts, 2017 (2017, August)

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See detailAn efficient algorithm to decide periodicity of b-recognisable sets using MSDF convention
Boigelot, Bernard ULg; Mainz, Isabelle ULg; Marsault, Victor ULg et al

in Leibniz International Proceedings in Informatics (2017, August), 80

Given an integer base b>1, a set of integers is represented in base b by a language over {0,1,...,b-1}. The set is said to be b-recognisable if its representation is a regular language. It is known that ... [more ▼]

Given an integer base b>1, a set of integers is represented in base b by a language over {0,1,...,b-1}. The set is said to be b-recognisable if its representation is a regular language. It is known that eventually periodic sets are b-recognisable in every base b, and Cobham's theorem implies the converse: no other set is b-recognisable in every base b. We are interested in deciding whether a $b$-recognisable set of integers (given as a finite automaton) is eventually periodic. Honkala showed that this problem is decidable in 1986 and recent developments give efficient decision algorithms. However, they only work when the integers are written with the least significant digit first. In this work, we consider the natural order of digits (Most Significant Digit First) and give a quasi-linear algorithm to solve the problem in this case. [less ▲]

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See detailNETPerfTrace – Predicting Internet Path Dynamics and Performance with Machine Learning
Wassermann, Sarah ULg; Casas, Pedro; Cuvelier, Thibaut ULg et al

in Proceedings of Big-DAMA ’17 (2017, August)

We study the problem of predicting Internet path changes and path performance using traceroute measurements and machine learning models. Path changes are frequently linked to path inflation and ... [more ▼]

We study the problem of predicting Internet path changes and path performance using traceroute measurements and machine learning models. Path changes are frequently linked to path inflation and performance degradation, therefore the relevance of the problem. We introduce NETPerfTrace, an Internet Path Tracking system to forecast path changes and path latency variations. By relying on decision trees and using empirical distribution-based input features, we show that NETPerfTrace can predict (i) the remaining life time of a path before it actually changes and (ii) the number of path changes in a certain time period with relatively high accuracy. Through extensive evaluation, we demonstrate that NETPerfTrace highly outperforms DTRACK, a previous system with the same prediction targets. NETPerfTrace also offers path performance forecasting capabilities. In particular, our tool can predict path latency metrics, providing a system which can not only predict path changes, but also forecast their impact in terms of performance variations. We release NETPerfTrace as open software to the networking community, as well as all evaluation datasets. [less ▲]

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See detailGiving an account of fictional discourse. Meinongian logics versus modal logics
Leclercq, Bruno ULg

Conference (2017, August)

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See detailRegiospecific radiolabelling of Nanofitin on Ni Magnetic Beads with [18F]FBEM and in vivo PET studies
Dammicco, Sylvestre ULg; Goux, Marine; Lemaire, Christian ULg et al

in Nuclear Medicine & Biology (2017)

Introduction: Nanofitins are low molecular weight, single chain and cysteine-free protein scaffolds able to selectively bind a defined biological target. They derive from Sac7d bacterial protein family ... [more ▼]

Introduction: Nanofitins are low molecular weight, single chain and cysteine-free protein scaffolds able to selectively bind a defined biological target. They derive from Sac7d bacterial protein family and are highly stable over a wide range of pH (0-13) and temperature (Tm ~80°C). Their extreme stability, low cost of production and high tolerability for chemical coupling make Nanofitins a very interesting alternative to antibodies and their fragments. Here, a hexahistidine tagged model Nanofitin (H4) directed against hen egg white lysozyme was radiolabelled and injected in mice to provide a baseline biodistribution and pharmacokinetic profiles to support future Nanofitin development programs. Method: A single cysteine residue has been genetically inserted in a model Nanofitin and its regioselective radiolabelling has been performed with 4-[18F]fluorobenzamido-N-ethylamino-maleimide ([18F]FBEM). The synthesis of [18F]FBEM has been completely implemented on a radiosynthesis unit (FastLab) including HPLC purification and formulation. Coupling with the [18F]FBEM has been achieved on a solid support (Ni magnetic beads) allowing rapid purification at room temperature without organic solvent. PET-MRI studies on C57BL/6 mice were conducted after injection of [18F]FBEM-Cys-H4 in order to access the biodistribution of this Nanofitin model. Results: Radiochemical yield (decay corrected) of 54±7% (n=4) was obtained after optimization for coupling the [18F]FBEM to Nanofitin. Pharmacokinetics results of [18F]FBEM-Cys-H4 revealed a fast clearance through the liver and the kidneys. Conclusion: An efficient new method on Ni magnetic beads was developed to radiolabelled his-tagged biomolecules with [18F]FBEM. This procedure was applied on a Nanofitin model Cys-H4 and biodistribution kinetic studies were achieved to evaluate the potential use of Nanofitin for diagnostic imaging. Fast clearance indicates that Nanofitins represent very interesting tools for diagnostic imaging. [less ▲]

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See detailForeseeing New Control Challenges in Electricity Prosumer Communities
Olivier, Frédéric ULg; Marulli, Daniele; Ernst, Damien ULg et al

in Proc. of the 10th Bulk Power Systems Dynamics and Control Symposium – IREP’2017 (2017, August)

This paper is dedicated to electricity prosumer communities, which are groups of people producing, sharing and consuming electricity locally. This paper focuses on building a rigorous mathematical ... [more ▼]

This paper is dedicated to electricity prosumer communities, which are groups of people producing, sharing and consuming electricity locally. This paper focuses on building a rigorous mathematical framework in order to formalise sequen- tial decision making problems that may soon be encountered within electricity prosumer communities. After introducing our formalism, we propose a set of optimisation problems reflecting several types of theoretically optimal behaviours for energy exchanges between prosumers. We then discuss the advantages and disadvantages of centralised and decentralised schemes and provide illustrations of decision making strategies, allowing a prosumer community to generate more distributed electricity (compared to commonly applied strategies) by mitigating over- voltages over a low-voltage feeder. We finally investigate how to design distributed control schemes that may contribute reaching (at least partially) the objectives of the community, by resort in to machine learning techniques to extract, from centralised solution(s), decision making patterns to be applied locally. First empirical results show that, even after a post-processing phase so that it satisfies physical constraints, the learning approach still performs better than predetermined strategies targeting safety first, then cost minimisation. [less ▲]

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See detailItem purification versus adjustments for multiple comparisons in DIF detection
Drabinova, Adela; Martinkova, Patricia; Magis, David ULg

Poster (2017, July 18)

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See detailmstR: An R package to generate multistage testing designs
Magis, David ULg; Yan, Duanli; von Davier, Alina

Conference (2017, July 18)

Multistage testing (MST; Yan, von Davier & Lewis, 2014) has become an important framework of tailored testing. Similarly to computerized adaptive testing (CAT) it proposes an optimal routing of the ... [more ▼]

Multistage testing (MST; Yan, von Davier & Lewis, 2014) has become an important framework of tailored testing. Similarly to computerized adaptive testing (CAT) it proposes an optimal routing of the administered items according to the previous test taker’s responses. However, items are not selected and administered as single units but as modules (subsets). The main goal of MST consists in selecting the optimal path of modules across the successive stages of the test. Unfortunately, if various operational testing programs are nowadays considering MST for practical administrations, there is still very limited access to accurate software that can either treat or generate MST scenarios for research purposes. In this talk we succinctly present a new package from the R software, called mstR (Magis, Yan & von Davier, 2017). Built in the same spirit of the package catR for CAT designs, it permits to generate repeated response patterns under a predefined MST scenario by providing the set of modules and related item parameters, the number of stages and the connections between modules from successive stages. Several rules for optimal module selection and ability estimation (under IRT framework or based on test scores) are also available. This talk will mostly focus on the (non-technical) description of package mstR and its main features. An example will also be described as an illustration of this framework. [less ▲]

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See detailRhétorique du détournement vidéoludique. Le cas de Pokémon
Barnabé, Fanny ULg

Doctoral thesis (2017)

La présente recherche se situe au croisement de plusieurs traditions disciplinaires – les sciences du jeu, les études littéraires, la rhétorique, les cultural studies – et de plusieurs objets – le jeu, le ... [more ▼]

La présente recherche se situe au croisement de plusieurs traditions disciplinaires – les sciences du jeu, les études littéraires, la rhétorique, les cultural studies – et de plusieurs objets – le jeu, le détournement, les fanfictions, machinimas, speedruns, let’s plays et mods. Sans prétendre opérer ici leur synthèse ou leur conciliation, nous tenterons de dégager, entre ces différents domaines, de nouvelles ouvertures : nous montrerons, en circulant parmi eux, qu’ils sont pris dans des tensions, des dynamiques, des questionnements similaires et, surtout, centraux pour la compréhension de la culture populaire contemporaine. Plusieurs objectifs nous animent : définir le détournement et, à travers lui, éclairer sous un autre jour le fonctionnement du jeu ; développer un métadiscours à même de soutenir l’analyse des œuvres produites par les joueurs ; enfin, par la même occasion, défendre l’intérêt d’étudier de près ces œuvres amateurs, trop souvent délaissées par la recherche au profit des pratiques, et qui sont pourtant des épicentres essentiels autour desquels se développent les cultures ludiques. La poursuite de ces objectifs, toutefois, ne va pas sans soulever des questions complexes, qui traversent toute la thèse : quels parallèles et quelle frontière établir entre le détournement et le jeu ? Comment une œuvre est-elle reconnue, étiquetée, classée comme un détournement ? Qu’est-ce au juste qu’une œuvre dans un domaine où l’intertextualité, la reprise, la réinterprétation sont des normes ? Avec quels outils décrire les formes du détournement, et qu’ont en commun les genres disparates regroupés sous cette dénomination ? Nous traiterons ces diverses interrogations en deux temps. La première partie de la thèse, qui comporte deux sous-sections, en constituera le socle théorique : un premier chapitre y aborde les relations intimes entre jeu et détournement, tout en resituant notre recherche dans les différents champs qu’elle fait se croiser ; le second chapitre s’attache à la constitution d’un cadre métadiscursif inspiré de la rhétorique, visant à définir ce que pourraient être des « figures » du détournement vidéoludique. La deuxième partie, plus analytique, servira d’application au cadre précédemment construit, en montrant son utilité sur des études d’œuvres concrètes. Celles-ci sont réparties en trois grandes catégories : les détournements par le play (let’s plays, speedruns, tool-assisted speedruns et machinimas), les détournements du game (fanfictions et mods) et les recontextualisations (« Twitch plays… »). Par ailleurs, en guise de fil conducteur, toutes ces œuvres – de formes et de genres variés – auront pour point commun essentiel d’être dérivées d’un même univers fictionnel : celui de la licence Pokémon. [less ▲]

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See detailH0LiCOW - I. H0 Lenses in COSMOGRAIL's Wellspring: program overview
Suyu, S. H.; Bonvin, V.; Courbin, F. et al

in Monthly Notices of the Royal Astronomical Society (2017), 468

Strong gravitational lens systems with time delays between the multiple images allow measurements of time-delay distances, which are primarily sensitive to the Hubble constant that is key to probing dark ... [more ▼]

Strong gravitational lens systems with time delays between the multiple images allow measurements of time-delay distances, which are primarily sensitive to the Hubble constant that is key to probing dark energy, neutrino physics and the spatial curvature of the Universe, as well as discovering new physics. We present H0LiCOW (H[SUB]0[/SUB] Lenses in COSMOGRAIL's Wellspring), a program that aims to measure H[SUB]0[/SUB] with <3.5 per cent uncertainty from five lens systems (B1608+656, RXJ1131-1231, HE 0435-1223, WFI2033-4723 and HE 1104-1805). We have been acquiring (1) time delays through COSMOGRAIL and Very Large Array monitoring, (2) high-resolution Hubble Space Telescope imaging for the lens mass modelling, (3) wide-field imaging and spectroscopy to characterize the lens environment and (4) moderate-resolution spectroscopy to obtain the stellar velocity dispersion of the lenses for mass modelling. In cosmological models with one-parameter extension to flat Λ cold dark matter, we expect to measure H[SUB]0[/SUB] to <3.5 per cent in most models, spatial curvature Ω[SUB]k[/SUB] to 0.004, w to 0.14 and the effective number of neutrino species to 0.2 (1σ uncertainties) when combined with current cosmic microwave background (CMB) experiments. These are, respectively, a factor of ˜15, ˜2 and ˜1.5 tighter than CMB alone. Our data set will further enable us to study the stellar initial mass function of the lens galaxies, and the co-evolution of supermassive black holes and their host galaxies. This program will provide a foundation for extracting cosmological distances from the hundreds of time-delay lenses that are expected to be discovered in current and future surveys. [less ▲]

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See detailProbing the atmosphere of a sub-Jovian planet orbiting a cool dwarf
Sedaghati, Elyar; Boffin, Henri M. J.; Delrez, Laetitia et al

in Monthly Notices of the Royal Astronomical Society (2017), 468

We derive the 0.01 $\mu$m binned transmission spectrum, between 0.74 and 1.0 $\mu$m, of WASP-80b from low resolution spectra obtained with the FORS2 instrument attached to ESO's Very Large Telescope. The ... [more ▼]

We derive the 0.01 $\mu$m binned transmission spectrum, between 0.74 and 1.0 $\mu$m, of WASP-80b from low resolution spectra obtained with the FORS2 instrument attached to ESO's Very Large Telescope. The combination of the fact that WASP-80 is an active star, together with instrumental and telluric factors, introduces correlated noise in the observed transit light curves, which we treat quantitatively using Gaussian Processes. Comparison of our results together with those from previous studies, to theoretically calculated models reveals an equilibrium temperature in agreement with the previously measured value of 825K, and a sub-solar metallicity, as well as an atmosphere depleted of molecular species with absorption bands in the IR ($\gg 5\sigma$). Our transmission spectrum alone shows evidence for additional absorption from the potassium core and wing, whereby its presence is detected from analysis of narrow 0.003 $\mu$m bin light curves ($\gg 5\sigma$). Further observations with visible and near-UV filters will be required to expand this spectrum and provide more in-depth knowledge of the atmosphere. These detections are only made possible through an instrument-dependent baseline model and a careful analysis of systematics in the data. [less ▲]

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See detailFreeplay-induced limit cycle oscillation mitigation using linear and nonlinear tuned vibration absorbers
Verstraelen, Edouard ULg; Kerschen, Gaëtan ULg; Dimitriadis, Grigorios ULg

in Proceeding of the IFASD 2017 Conference (2017, July)

Structural nonlinearities such as freeplay in control surface bearings and actuators or in connections between wings and external payloads sometimes lead to aeroelastic limit cycle oscillations at ... [more ▼]

Structural nonlinearities such as freeplay in control surface bearings and actuators or in connections between wings and external payloads sometimes lead to aeroelastic limit cycle oscillations at airspeeds lower than the linear flutter speed of the aircraft. In parallel, numerous studies demonstrated the potential of linear and nonlinear tuned vibration absorbers to increase the flutter speed of linear and continuously hardening aeroelastic systems such as two-degree- of-freedom wings or long span bridges. In this work, the effect of linear and nonlinear tuned vibration absorbers is studied on a wing with pitch plunge and control surface deflection degrees of freedom and with freeplay in pitch. Depending on the tuning of the linear absorber, the linear flutter speed of the system can be increased by 10% or the onset of limit cycle oscillations due to the freeplay can be delayed by 7.7% and their amplitude can be significantly decreased. The addition of cubic hardening forces on the absorber can further decrease the limit cycle amplitude in a limited airspeed range at the cost of an increase in limit cycle amplitude in another airspeed range. Conversely, the addition of a freeplay hardening force on the absorber can decrease the limit cycle amplitude without any detrimental effect. [less ▲]

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