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See detailEtude du rôle des sites de N-glycosylation dans la pathogenèse induite par le virus de la leucémie bovine
De Brogniez, Alix ULg

Doctoral thesis (2014)

Le virus de la leucémie bovine (BLV) est un deltarétrovirus responsable d’une maladie lymphoproliférative chez le bovin. Cette maladie, appelée leucose bovine enzootique, est responsable de pertes ... [more ▼]

Le virus de la leucémie bovine (BLV) est un deltarétrovirus responsable d’une maladie lymphoproliférative chez le bovin. Cette maladie, appelée leucose bovine enzootique, est responsable de pertes économiques importantes dans l’industrie laitière et viandeuse de nombreux pays dont notamment les Etats-Unis, l’Argentine et le Brésil. Ce rétrovirus infecte les lymphocytes B à l’aide de ses glycoprotéines virales SU et TM qui fusionnent avec la membrane cellulaire. Bien que de nombreuses études se soient penchées sur la caractérisation de la propagation virale, les composants cellulaires et viraux impliqués dans le contrôle de ce mécanisme ne sont toujours pas élucidés. Mon projet de thèse s’est donc attaché à la compréhension du rôle joué par les 8 sites potentiels de glycosylation présents sur la protéine SU du BLV. Chaque site du gène a été muté individuellement par mutagenèse dirigée. L’expression, la localisation, la capacité de fusion cellulaire et la stabilité protéique ont d’abord été étudiées en culture. Des provirus mutés ont ensuite été inoculés à des moutons afin d’évaluer leur potentiel infectieux et de suivre l’évolution de la pathogenèse induite. Nos études ont permis de mettre en évidence un mécanisme de glycosylation qui limite la réplication virale et ralentit la pathogenèse en faveur de la persistance virale. [less ▲]

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See detailMulti-period stochastic optimization problems in transportation management (Ph. D. Thesis Summary)
Pironet, Thierry ULg

in 4OR : Quarterly Journal of the Belgian, French and Italian Operations Research Societies (2014)

Ph. D Thesis summary see other reference on ORBI

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See detailQue sont devenus les manuels d'histoire d'antan ? Pour un nouveau concept de manuel scolaire
Jadoulle, Jean-Louis ULg

Conference given outside the academic context (2014)

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See detailCoordination of alkali metal ions to model branched hexasaccharides dictates fragment yield in MALDI in-source decay with hydrogen abstraction using 5-nitrosalicylic acid as the matrix
Asakawa, Daiki; Smargiasso, Nicolas ULg; De Pauw, Edwin ULg

in Journal of Mass Spectrometry [=JMS] (2014), 49

The differences among MALDI-ISD spectra of hexasaccharides cationized with various alkali metals are reflected in the different stabilities of the ‘hydrogen-deficient’ glycan radical. The stability order ... [more ▼]

The differences among MALDI-ISD spectra of hexasaccharides cationized with various alkali metals are reflected in the different stabilities of the ‘hydrogen-deficient’ glycan radical. The stability order of alkali metal-glycan radical complexes is proportional to the size of the alkali metal cation, K+ > Na+ > Li+. The Coordination of alkali metal ions to model branched hexasaccharides is the major parameter that dictates the fragment yield in MALDI in-source decay with hydrogen abstraction using 5-nitrosalicylic acid as the matrix [less ▲]

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See detailFrench good practice guidelines for the management of the low back pain risk among workers exposed to manual handling
Petit, Audrey; Mairiaux, Philippe ULg; FASSIER, Jean-Baptiste et al

Poster (2014, September 29)

Background Manual handling of loads (MHL) is a widespread practice in the workplace and is the leading cause of work accidents. In particular, MHL is associated with a risk of low back injuries which can ... [more ▼]

Background Manual handling of loads (MHL) is a widespread practice in the workplace and is the leading cause of work accidents. In particular, MHL is associated with a risk of low back injuries which can compromise staying at work and worker's career in the case of recurrence or chronicity. This communication aims to set forth the French occupational Guidelines for the management of the low back pain (LBP) risk among workers exposed to MHL, published in October 2013. Methods These Good Practice Guidelines were written according to the Clinical Practice Guidelines method proposed by the French National Authority for Health. They emanated from a synthesis of the literature by a multidisciplinary working party of 24 experts and were peer reviewed by a committee of 50 experts. These Guidelines are designed to define the components of an appropriate surveillance program for all workers exposed to MHL activities at the workplace and especially suffering from LBP and still at work, or suffering LBP and on sick leave. Results Because of the potential impact on workers’ fears and beliefs, the information provided by the various actors must be consistent, or even reassuring in relation to the prognosis of LBP. Among LBP workers, it is recommended to look for signs of severity or an underlying disease at the acute, subacute and chronic stages of LBP; to encourage continuation or resumption of physical activity; situate the current episode of LBP in the worker’s work history (recent changes of working conditions) and evaluate the impact of LBP on the worker’s job. In the case of persistent or recurrent LBP, it is recommended to evaluate biopsychosocial and socioeconomic risk factors likely to influence chronicity, prolonged incapacity and delay return to work. If workers are on prolonged and/or repeated sick leave for LBP, a pre-return-to-work visit is recommended to evaluate the pain and functional disability and their repercussions, as well as the main factors of prolonged work incapacity; encourage and help the worker to develop a return to work dynamic; identify the main difficulties related to work and possible job adjustments and evaluate the need to initiate a job staying at work approach. Conclusions Primary, secondary and tertiary prevention are necessary for the management of low back pain at work. [less ▲]

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See detailDétermination de la durabilité des complexes parois et toitures vis-à-vis d’attaques biologiques
Jourez, Benoît ULg; Hebert, Jacques ULg; Christiaens, Christophe ULg et al

Conference given outside the academic context (2014)

Le secteur de la construction est en perpétuel évolution et de nouveaux systèmes constructifs sont proposés aux candidats bâtisseurs. Ils vissent à répondre aux nouvelles exigences de performance ... [more ▼]

Le secteur de la construction est en perpétuel évolution et de nouveaux systèmes constructifs sont proposés aux candidats bâtisseurs. Ils vissent à répondre aux nouvelles exigences de performance (mécanique, acoustique, thermique), de développement durable ainsi qu'à des exigences économiques. Néanmoins, ces nouveaux systèmes constructifs innovants, pour lesquels un manque de recul est observé, doivent encore démontrer leur capacité à maintenir dans le temps leur efficacité initiale et leur durabilité afin d'aboutir à des systèmes performants, durables et sans risques de pathologie. A ce titre, ces complexes, la paroi perspirante et la toiture plate compacte, doivent faire l'objet d'investigations en vue d'évaluer leur durabilité vis-à-vis des attaques par les agents de dégradation biologique. La tendance actuelle s'oriente vers des complexes constitués en majorité de matériaux biodégradables (bois, panneau, isolant naturel) tout en évitant l'emploi de produits nocifs pour la santé des habitants qui sont de plus en plus conscients de leur empreinte écologique et réticents à l'incorporation de substances chimiques potentiellement nocives. Il est à noter cependant que les constituants de ces complexes bien que biodégradables doivent aussi faire preuve d'une durabilité suffisante qui ne devrait pas être limitée à la durée de la garantie décennale assumée par le concepteur/entrepreneur du bâtiment mais s'étendre à la durée de vie de celui-ci. L'origine du projet OPTIDUBO repose sur le fait que le comportement hygroscopique de ces complexes et leur durabilité face à l'attaque des agents biologiques de dégradation (insectes xylophages et champignons lignivores) sont peu connus ou documentés alors que ceux-ci sont de plus en plus utilisés dans la construction d'habitations. Ce chapitre se focalise sur la détermination de la durabilité des complexes parois perspirantes et toiture plate compacte vis-à-vis des agents biologiques de dégradation. Il vise à répondre aux deux questions suivantes:  Dans ces complexes, un ou plusieurs des constituants de la paroi sont-ils attaquables ?  Le traitement de l'isolant au sel de bore est-il susceptible de protéger efficacement le complexe de toute attaque? [less ▲]

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See detailTheoretical studies of PbTiO3 and SrTiO3 under uniaxial mechanical constraints combining first-principles calculations and phenomenological Landau theory
Sharma, Henu ULg

Doctoral thesis (2014)

In the present thesis we present theoretical studies of perovskite compounds under uniaxial mechanical constraints combining first-principles DFT calculations approach and phenomenological Landau theory ... [more ▼]

In the present thesis we present theoretical studies of perovskite compounds under uniaxial mechanical constraints combining first-principles DFT calculations approach and phenomenological Landau theory. ABO$_3$ perovskites form a very important class of functional materials that can exhibit a broad range of properties (e.g., superconductivity, magnetism, ferroelectricity, multiferroism, metal-insulator transitions\ldots) within small distortions of the same simple prototype cubic structure. Though these compounds have been extensively studied both experimentally and computationally, there are still unresolved issues regarding the effect of pressure. In recent years, strain engineering has reported to be an original approach to tune the ferroelectric properties of perovskite ABO$_3$ compounds. While the effect of epitaxial biaxial strain and hydrostatic strain is rather well understood in this class of materials, very little is yet known regarding the effect of uniaxial mechanical constraints. Our study is motivated by the little existing understanding of the effect of uniaxial strain and stress, that has been up to now almost totally neglected. Two prototype compounds are studied in detail: PbTiO$_3$ and SrTiO$_3$. After a general introduction on ABO$_3$ compounds and calculations techniques (ab initio and phenomenological Landau model), we studied the effect of mechanical constraints in these compounds in our thesis. PbTiO$_3$ is a prototypical ferroelectric compound and also one of the parent components of the Pb(Zr,Ti)O$_3$ solid solution (PZT), which is the most widely used piezoelectrics. For PbTiO$_3$, we have shown that irrespectively of the uniaxial mechanical constraint applied, the system keeps a purely ferroelectric ground-state, with the polarization aligned either along the constraint direction ($FE_z$ phase) or along one of the pseudo-cubic axis perpendicular to it ($FE_x$ phase). This contrasts with the case of isotropic or biaxial mechanical constraints for which novel phases combining ferroelectric and antiferrodistortive motions have been previously reported. Under uniaxial strain, PbTiO$_3$ switches from a $FE_x$ ground state under compressive strain to $FE_z$ ground-state under tensile strain, beyond a critical strain $\eta_{zz}^c \approx +1$\%. Under uniaxial stress, PbTiO$_3$ exhibits either a $FE_x$ ground state under compression ($\sigma_{zz} < 0$) or a $FE_z$ ground state under tension ($\sigma_{zz} > 0$). Here, however, an abrupt jump of the structural parameters is also predicted under both compressive and tensile stresses at critical values $\sigma_{zz} \approx$ $+2$ GPa and $- 8$ GPa. This behavior appears similar to that predicted under negative isotropic pressure and might reveal practically useful to enhance the piezoelectric response in nanodevices. The second compound of interest is SrTiO$_3$. It has been widely studied in the past decades due to its unusual properties at low temperature. In this work, we have extended our previous investigations on PbTiO$_3$ by exploring theoretically the pressure effects on perovskite SrTiO$_3$ combining the first-principles calculations and a phenomenological Landau model. We have discussed the evolution of phonon frequencies of SrTiO$_3$ with the three isotropic, uniaxial and biaxial strains using first-principles calculations. We also reproduce the previous work done in SrTiO$_3$ with epitaxial strain and hydrostatic strain. Finally, we have calculated the phase diagram of SrTiO$_3$ under uniaxial strain, as obtained from Landau theory and discussed how it compares with the first-principles calculations. [less ▲]

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See detailFITNESS EVALUATION OF A RECOMBINANT MURINE NOROVIRUS DURING SERIAL PASSAGES IN CELL CULTURE
Oliveira-Filho, Edmilson; Di Felice, Elisabetta; Toffoli, Barbara et al

Poster (2014, September 28)

Noroviruses are single stranded positive sense RNA viruses which can infect human and different animal species. Human norovirus (NoV) infections are among the most important causes of gastroenteritis in ... [more ▼]

Noroviruses are single stranded positive sense RNA viruses which can infect human and different animal species. Human norovirus (NoV) infections are among the most important causes of gastroenteritis in both children and adults. Infections often occur as outbreaks which may be foodborne. Due to the lack of an efficient cell culture system as well as a workable animal model, many aspects of the NoV infection in human are still poorly understood. The murine norovirus (MuNoV) grows easily in cell culture in contrast to the Human NoV, and constitutes an excellent animal model. Recombination can dramatically change virulence properties of the viruses and has been evidenced in silico for different human NoV strains isolated from clinical cases. Recently, after in vitro coinfection of RAW264.7 cells with parental MuNoV strains CW1 and Wu20, we obtained a recombinant Wu20/CW1 strain. This recombinant strain showed reduced plaque size compared to the parental strains. The aim of the study was to observe and molecularly characterize the natural genetic evolution of the recombinant MuNoV strain across in vitro replications. Viral fitness is a complex concept. Here we defined this fitness as the ability of a viral population to adapt to the cell culture system. Thus, the recombinant strain was serially replicated in vitro in RAW264.7 cells (up to 14 passages). Viral plaque sizes of early and late progenies were compared with the Image J software. A significant difference was shown between them with the Mann and Whitney non parametric statistical test. Afterwards, viruses from different cell passages were cloned and sequenced. The average plaque size increased from the earlier to the later progenies (from 0.1 mm2 to around 0.5 mm2). Molecular investigations are currently performed in order to specify in which genetic region mutations occur and whether or not this could explain fitness modifications during in vitro evolution. In addition, two other parameters of in vitro virulence modification will be investigated: (i) virus production and (ii) one step growth kinetics. The data should provide interesting information about genetic evolution in the genus Norovirus, especially regarding recombination events and explain how a recombinant strain, first disadvantaged compared to its parental strains, could regain fitness by genetic evolution. [less ▲]

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See detailLe rôle des historiens dans le processus de reconversion des sites industriels en Wallonie
Destatte, Julien ULg; Defechereux, Olivier ULg; Peters, Arnaud ULg

Conference given outside the academic context (2014)

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See detailDie Rezeption Thibauts in Belgien und Frankreich
Gerkens, Jean-François ULg

Conference (2014, September 27)

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See detailDysphasie : Réflexions autour de la définition et des critères diagnostiques
Leclercq, Anne-Lise ULg; Maillart, Christelle ULg

in Les entretiens de Bichat : les entretiens d'orthophonie 2014 : Les "dys", état de l'art et orientations cliniques (2014, September 27)

Specific language impairment refers to a non-homogeneous group: linguistic and non-linguistic abilities differ from one child to another. Increasing knowledge in this field has led to clarify both the ... [more ▼]

Specific language impairment refers to a non-homogeneous group: linguistic and non-linguistic abilities differ from one child to another. Increasing knowledge in this field has led to clarify both the definition and the diagnostic criteria in order to facilitate its diagnosis. However, the clinical complexity of this trouble questions the relevance of these diagnostic criteria. The present article intends to think about the clinical diagnostic of specific language impairment in order to allow informed decision making during its assessment and therapy. [less ▲]

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See detailFORMATION: Atelier clinique en prise en charge neuropsychologique
Adam, Stéphane ULg

Diverse speeche and writing (2014)

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See detailL'harmonisation des statuts entre ouvriers et employés
Baukens, Michèle; Rozenberg, Fanny ULg; Kéfer, Fabienne ULg

Book published by Anthemis (2014)

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See detailAgricultures de demain? Produire avec la nature...
Hatt, Séverin ULg; Brédart, David ULg

Conference given outside the academic context (2014)

Baisse de la biodiversité, pollution de l’eau, crises alimentaires, diminution du nombre d’agriculteurs… ces sujets régulièrement présents dans l’actualité remettent en question le modèle industriel de la ... [more ▼]

Baisse de la biodiversité, pollution de l’eau, crises alimentaires, diminution du nombre d’agriculteurs… ces sujets régulièrement présents dans l’actualité remettent en question le modèle industriel de la production agricole. Nous présenterons des alternatives actuellement développées par des agriculteurs et des chercheurs. Nous proposons, par exemple, de découvrir des dispositifs qui réduisent l’utilisation de pesticides ou de fertilisants. [less ▲]

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See detailEFFICACY and Characterization of THE LACTOPEROXIDASE SYSTEM, A NATURAL BIOLOGICAL PESTICIDE
Bafort, Françoise ULg; Perraudin, Jean-Paul; Jijakli, Haissam ULg

Conference (2014, September 26)

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See detailDetection of non-concave and non-increasing spectra: Snu spaces revisited with wavelet leaders
Esser, Céline ULg

Conference (2014, September 26)

Our objective is to study the pointwise regularity of functions via their multifractal spectrum. Computing the multifractal spectrum of a function using directly its definition is an unattainable goal in ... [more ▼]

Our objective is to study the pointwise regularity of functions via their multifractal spectrum. Computing the multifractal spectrum of a function using directly its definition is an unattainable goal in most of the practical cases, but there exist heuristic methods, called multifractal formalisms, which allow to estimate this spectrum and which give satisfactory results in many situations. The Frisch-Parisi conjecture, classically used and based on Besov spaces, presents two disadvantages: it can only hold for spectra that are concave and it can only yield the increasing part of spectra. Concerning the first problem, the use of S spaces allows to deal with non-concave increasing spectra. Concerning the second problem, a generalization of the Frisch-Parisi conjecture obtained by replacing the role played by wavelet coefficients by wavelet leaders allows to recover the decreasing part of concave spectra. We present a combination of both approaches to define a new formalism derived from large deviations based on statistics of wavelet leaders. We also present the associated function space. [less ▲]

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See detailGenericity and classes of ultradifferentiable functions
Esser, Céline ULg

Conference (2014, September 26)

As surprising as it may seem, there exist infinitely differentiable functions which are nowhere analytic. When such an unexpected object is found, a natural question is to ask whether many similar ones ... [more ▼]

As surprising as it may seem, there exist infinitely differentiable functions which are nowhere analytic. When such an unexpected object is found, a natural question is to ask whether many similar ones may exist. A classical technique is to use the Baire category theorem and the notion of residuality. This notion is purely topological and does not give any information about the measure of the set of objects satisfying such a property. In this purpose, the notion of prevalence has been introduced. Moreover, one could also wonder whether large algebraic structures of such objects can be constructed. This question is formalized by the notion of lineability. The first objective of this talk is to go further into the study of nowhere analytic functions. It is known that the set of nowhere analytic functions is residual and lineable in C^infty([0, 1]). We prove that the set of nowhere analytic functions is also prevalent in this space. Those results of genericity are then generalized using Gevrey classes, which can be seen as intermediate between the space of analytic functions and the space of infinitely differentiable functions. We also study how far such results of genericity could be extended to spaces of ultradifferentiable functions, defined using weight sequences. [less ▲]

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