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See detailSystemic biomarkers of collagen and elastin turnover are associated with clinically relevant outcomes in COPD.
Stolz, Daiana; Leeming, Diana Julie; Edfort Kristensen, Jacob Hull et al

in CHEST (2017)

BACKGROUND: Extracellular matrix remodeling (ECM) of the lung tissue releases protein fragments into the blood, where they may be detected as serological surrogate markers of disease activity in chronic ... [more ▼]

BACKGROUND: Extracellular matrix remodeling (ECM) of the lung tissue releases protein fragments into the blood, where they may be detected as serological surrogate markers of disease activity in chronic obstructive pulmonary disease (COPD). We aimed to assess the association of ECM turnover with severity and outcome of COPD. METHODS: In a prospective, observational, multicenter study including 506 patients with COPD, GOLD grades II-IV, we analyzed serum samples at stable state, exacerbation and 4 weeks after exacerbation, for a panel of 5 novel neo-epitopes including fragments of collagen type-III (C3M) and type-VI (C6M), pro-forms of collagen type-III (Pro-C3) and type-VI (Pro-C6) and neutrophil elastase-generated fragments of elastin (EL-NE) by ELISA. These neo-epitopes were also measured at stable state in a derivation cohort including 100 COPD patients. RESULTS: Serum levels of C3M, C6M, Pro-C3, Pro-C6 and EL-NE were associated with lung function. Patients with the lowest levels of Pro-C3 and Pro-C6 had more severe airflow limitation, hyperinflation, air trapping, and emphysema. Degradation of collagen type-III and -VI was associated with dyspnea. All ECM biomarkers, except Pro-C6, were increased at exacerbation as compared to stable state but, except EL-NE, did not differ between stable state and exacerbation follow-up in the crude and adjusted analyses. In Cox regression adjusted analyses, Pro-C3 was associated with a shorter time to exacerbation (HR 0.72[0.59-0.89] p=0.002) and Pro-C6 with survival (HR 2.09[1.18-3.71], p=0.011). CONCLUSIONS: Serum biomarkers of ECM turnover are significantly associated with disease severity and clinically relevant outcomes in COPD. [less ▲]

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See detailThiamine and benfotiamine improve cognition and ameliorate GSK-3β-associated stressinduced behaviours in mice
Markova, Nataliia; Bazhenova, Nataliya; Anthony, Daniel C et al

in Progress in Neuro-Psychopharmacology & Biological Psychiatry (2017), 75

Thiamine deficiency in the brain has been implicated in the development of dementia and symptoms of depression. Indirect evidence suggests that thiamine may contribute to these pathologies by controlling ... [more ▼]

Thiamine deficiency in the brain has been implicated in the development of dementia and symptoms of depression. Indirect evidence suggests that thiamine may contribute to these pathologies by controlling the activities of glycogen synthase kinase (GSK)-3β. While decreased GSK- 3β activity appears to impair memory, increased GSK-3β activity is associated with the distressed/depressed state. However, direct evidence for the effects of thiamine on GSK-3β function were not have not been reported. Here, we administered thiamine or, the more bioavailable precursor, benfotiamine at 200 mg/kg/day for 2 weeks to C57BL/6J mice, to determine whether treatment might affect behaviours that are known to be sensitive to GSK-3β activity and whether such administration impacts on GSK-3β expression within the brain. The mice were tested in models of contextual conditioning and extinction, a 5-day rat exposure stress test, and a modified swim test with repeated testing. Imipramine (7.5 mg/kg/day) was administered as a positive control for thiamine or benfotiamine. As for imipramine, both compounds inhibited the upregulation of GSK-3β induced by predator stress or repeated swimming, and reduced floating scores and the predator stress-induced behavioural changes in anxiety and exploration. Coincident, thiamine and benfotiamine improved learning and extinction of contextual fear, and the acquisition of the step-down avoidance task. Together, our data indicate that thiamine and benfotiamine have antidepressant/anti-stress effects in naïve animals that are associated with reduced GSK-3β expression. Importantly, the treatment also had pro-cognitive actions despite the impact on GSK-3β activity. [less ▲]

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See detailThiamine: Biological forms and function
Bettendorff, Lucien ULg

Conference (2017)

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See detailMechanotransductive cascade of Myo-II-dependent mesoderm and endoderm invaginations in embryo gastrulation
Mitrossilis, Démosthène; Röper, Jens-Christian; Roy, Damien Le et al

in Nature Communications (2017), 8

Animal development consists of a cascade of tissue differentiation and shape change. Associated mechanical signals regulate tissue differentiation. Here we demonstrate that endogenous mechanical cues also ... [more ▼]

Animal development consists of a cascade of tissue differentiation and shape change. Associated mechanical signals regulate tissue differentiation. Here we demonstrate that endogenous mechanical cues also trigger biochemical pathways, generating the active morphogenetic movements shaping animal development through a mechanotransductive cascade of Myo-II medio-apical stabilization. To mimic physiological tissue deformation with a cell scale resolution, liposomes containing magnetic nanoparticles are injected into embryonic epithelia and submitted to time-variable forces generated by a linear array of micrometric soft magnets. Periodic magnetically induced deformations quantitatively phenocopy the soft mechanical endogenous snail-dependent apex pulsations, rescue the medio-apical accumulation of Rok, Myo-II and subsequent mesoderm invagination lacking in sna mutants, in a Fog-dependent mechanotransductive process. Mesoderm invagination then activates Myo-II apical accumulation, in a similar Fog-dependent mechanotransductive process, which in turn initiates endoderm invagination. This reveals the existence of a highly dynamic self-inductive cascade of mesoderm and endoderm invaginations, regulated by mechano-induced medio-apical stabilization of Myo-II. [less ▲]

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See detailAlgorithm for Temporal Gait Analysis Using Wireless Foot-Mounted Accelerometers
Boutaayamou, Mohamed ULg; Denoël, Vincent ULg; Bruls, Olivier ULg et al

Book published by Springer (2017)

We present a new signal processing algorithm that extracts five gait events: heel strike, toe strike, heel-off, toe-off, and heel clearance from only two accelerometers attached on the heels of the ... [more ▼]

We present a new signal processing algorithm that extracts five gait events: heel strike, toe strike, heel-off, toe-off, and heel clearance from only two accelerometers attached on the heels of the subjects usual shoes. This algorithm first uses a continuous wavelet-based segmentation that parses the signal of consecutive strides into motionless periods defining relevant local acceleration signals. Then, the algorithm uses versatile techniques to accurately extract the five gait events from these local acceleration signals. We validated, on a stride-by-stride basis, the extraction of these gait events by comparing the results with reference data provided by a kinematic 3D analysis system and a video camera. The accuracy and precision achieved by the extraction algorithm for healthy subjects, the reduced number of accelerometer units required, and the validation results obtained, encourage us to further study this system in pathological conditions. [less ▲]

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See detailA surprising and dramatic neuroendocrine-immune phenotype of mice deficient in Growth Hormone-Releasing Hormone (GHRH)
Farhat, Khalil; Bodart, Gwennaelle; Martens, Henri ULg et al

in Neuroimmunomodulation (2017)

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See detailEstrogen receptors and estetrol-dependent neuroprotective actions: a pilot study.
Tskitishvili, Ekaterine ULg; Pequeux, Christel ULg; Munaut, Carine ULg et al

in Journal of Endocrinology (2017), 232(1), 85-95

Estetrol (E4) has strong antioxidative, neurogenic and angiogenic effects in neural system resulting in the attenuation of neonatal hypoxic-ischemic encephalopathy. We aimed to define the role of estrogen ... [more ▼]

Estetrol (E4) has strong antioxidative, neurogenic and angiogenic effects in neural system resulting in the attenuation of neonatal hypoxic-ischemic encephalopathy. We aimed to define the role of estrogen receptors in E4-dependent actions in neuronal cell cultures and prove the promyelinating effect of E4. In vitro the antioxidative and cell survival/proliferating effects of E4 on H2O2-induced oxidative stress in primary hippocampal cell cultures were studied using different combinations of specific inhibitors for ERalpha (MPP dihydrochloride), ERbeta (PHTTP), GPR30 (G15) and palmytoilation (2-BR). LDH activity and cell survival assays were performed. In vivo the promyelinating role of different concentrations of E4 (1 mg/kg/day, 5 mg/kg/day, 10 mg/kg/day, 50 mg/kg/day) was investigated using the hypoxic-ischemic brain damage model in the 7-day-old immature rats before/after the induction of hypoxic-ischemic insult. Myelin basic protein (MBP) immunostaining was performed on brain coronal sections. Our results show that LDH activity is significantly upregulated in cell cultures where the E4's effect was completely blocked by concomitant treatment either with ERalpha and ERbeta inhibitors (MPP and PHTPP, respectively), or ERalpha and ERbeta inhibitors combined with 2-BR. Cell survival is significantly downregulated in cell cultures where the effect of E4 was blocked by ERbeta inhibitor (PHTTP) alone. The blockage of GRP30 receptor did affect neither LDH activity nor cell survival. MBP immunostaining is significantly upregulated in E4-pretreated groups at a concentration of 5 mg/kg/day and 50 mg/kg/day E4, whereas the MBP-positive area OD ratio is significantly increased in all the E4-treated groups. E4's antioxidative actions mostly depend on ERalpha and ERbeta, whereas neurogenesis and possibly promyelinating activities might be realized through ERbeta. [less ▲]

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See detailFrançois Ier et les arts du Nord (1515-1547). Des liens à explorer et à appronfondir encore
Fagnart, Laure ULg; Lecocq, Isabelle

in Fagnart, Laure; Lecocq, Isabelle (Eds.) François Ier et les arts du Nord (1515-1547) (2017)

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See detailPest and mould infestation of smoked shrimp and preventive measures for storage in cottage industry
Kpoclou; Anihouvi; Azokpota et al

in Indian Journal of Traditional Knowledge (2017), 16

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See detailJean Clouet vs Joos van Cleve
Fagnart, Laure ULg

in Fagnart, Laure; Lecocq, Isabelle (Eds.) François Ier et les arts du Nord (1515-1547) (2017)

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See detailThe Epoch of Smart Cities and Innovation.
Crutzen, Nathalie ULg; Marolla, Cesar

in Marolla, Cesar (Ed.) Climate Health Risks in Megacities: Sustainable Management and Strategic Planning (2017)

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See detailPost-scriptum à La Misère du monde 25 ans après : Bourdieu, le peuple et son suicide
Cormann, Grégory ULg

in Provenzano, François; Goin, Emilie (Eds.) Usages du peuple (2017)

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See detailOptimized approach to retrieve information on atmospheric carbonyl sulfide (OCS) above the Jungfraujoch station and change in its abundance since 1995
Lejeune, Bernard ULg; Mahieu, Emmanuel ULg; Vollmer, M. K. et al

in Journal of Quantitative Spectroscopy & Radiative Transfer (2017), 186

In this paper, we present an optimized retrieval strategy for carbonyl sulfide (OCS), using Fourier transform infrared (FTIR) solar observations made at the high-altitude Jungfraujoch station in the Swiss ... [more ▼]

In this paper, we present an optimized retrieval strategy for carbonyl sulfide (OCS), using Fourier transform infrared (FTIR) solar observations made at the high-altitude Jungfraujoch station in the Swiss Alps. More than 200 lines of the nu3 fundamental band of OCS have been systematically evaluated and we selected 4 microwindows on the basis of objective criteria minimizing the effect of interferences, mainly by solar features, carbon dioxide and water vapor absorption lines, while maximizing the information content. Implementation of this new retrieval strategy provided an extended time series of the OCS abundance spanning the 1995-2015 time period, for the study of the long-term trend and seasonal variation of OCS in the free troposphere and stratosphere. Three distinct periods characterize the evolution of the tropospheric partial columns: a first decreasing period (1995-2002), an intermediate increasing period (2002-2008), and the more recent period (2008-2015) which shows no significant trend. Our FTIR tropospheric and stratospheric time series are compared with new in situ gas chromatography mass spectrometry (GCMS) measurements performed by Empa (Laboratory for Air Pollution/Environmental Technology) at the Jungfraujoch since 2008, and with space-borne solar occultation observations by the ACE-FTS instrument on-board the SCISAT satellite, respectively, and they show good agreement. The OCS signal recorded above Jungfraujoch appears to be closely related to anthropogenic sulfur emissions. [less ▲]

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See detailEntrepreneurial teams in social entrepreneurship: When team heterogeneity facilitates organizational hybridity
Dufays, Frédéric ULg; Huybrechts, Benjamin ULg

in Ben-Hafaïedh, Cyrine; Cooney, Thomas (Eds.) Research handbook on entrepreneurial teams: Theory and practice (2017)

This chapter examines the composition of teams involved in social entrepreneurship and more particularly their heterogeneity in terms of “institutional logics”. It proposes that when social welfare and ... [more ▼]

This chapter examines the composition of teams involved in social entrepreneurship and more particularly their heterogeneity in terms of “institutional logics”. It proposes that when social welfare and market logics are integrated within founding teams, such hybridity is likely to infuse the whole entrepreneurial process to ultimately lead to the creation of a social enterprise as a “hybrid” organization. To theorize this process, a model is proposed that examines the implications of entrepreneurial team heterogeneity in social entrepreneurship. It makes clear that complexity and dynamism, in particular with regard to individuals’ social network structure, as well as interactions between team members, are necessary to understand the impact of team composition on the entrepreneurial process. An illustrative case study is presented to demonstrate the practical relevance of the model. [less ▲]

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See detailIntroduction
Masson, Christophe ULg

E-print/Working paper (2017)

Introduction de l'Assemblée générale de Ménestrel – 35e journée d'étude du Réseau des Médiévistes belges de Langue française. Le Moyen Âge dans les (nouveaux) médias. Quelle place pour les médiévistes  ... [more ▼]

Introduction de l'Assemblée générale de Ménestrel – 35e journée d'étude du Réseau des Médiévistes belges de Langue française. Le Moyen Âge dans les (nouveaux) médias. Quelle place pour les médiévistes ? Université de Liège, 7-8 octobre 2016 [less ▲]

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See detailApproche phénoménologique du soi psychopathique
Englebert, Jérôme ULg

in Cormann, Grégory; Boccaccini, Federico (Eds.) Le soi agent : Approches phénoménologiques (2017)

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See detailSustainability and management control. Exploring and theorizing control patterns in large European firms
Crutzen, Nathalie ULg; Zvezdov, Dimitar; Schaltegger, Stefan

in Journal of Cleaner Production (2017), 143

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See detailOn the Elaboration of Metal-Ceramic Composite Coatings by Laser Cladding
Mertens, Anne ULg; L'Hoest, Thibaut; Magnien, Julien et al

in Materials Science Forum (2017), 879

This paper reports on a preliminary investigation into the elaboration, by the additive process known as laser cladding, of composite coatings with a matrix of stainless steel 316L reinforced with varying ... [more ▼]

This paper reports on a preliminary investigation into the elaboration, by the additive process known as laser cladding, of composite coatings with a matrix of stainless steel 316L reinforced with varying contents of tungsten (WC) or silicon carbides (SiC) particles. Laser cladding is characterised by ultra-fast solidification and cooling rates, thus giving rise to ultra-fine out-of-equilibrium microstructures and potentially enhanced mechanical properties. Both types of composite coatings – i.e. with SiC or WC ‒ are compared in terms of their microstructures and hardness. Special attention is given to the dissolution of the carbides particles and to interfacial reactions taking place between the particles and the metallic matrix. [less ▲]

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See detailDEVELOPMENT OF ANALYTICAL TOOLS FOR THE DETERMINATION OF MARKERS OF LIPID ALTERATION IN FOOD
Douny, Caroline ULg

Doctoral thesis (2017)

It is now well-known that n-3 polyunsaturated fatty acids (omega-3 PUFA) have beneficial effects on health but are also rapidly oxidized in potentially toxic compounds, which means that healthy food ... [more ▼]

It is now well-known that n-3 polyunsaturated fatty acids (omega-3 PUFA) have beneficial effects on health but are also rapidly oxidized in potentially toxic compounds, which means that healthy food products, rich in n-3 PUFA, can become toxic for the consumer if not stored or processed appropriately. The main objective of this work was to develop new analytical tools to study markers of lipids alteration in food, in order to allow an adequate monitoring of possible toxic compounds in n-3 PUFA rich food products. The first chapter of this PhD thesis is a literature review about fatty acids, their oxidation and the different oxidation products generated. The different analytical techniques used to study the oxidative stability of polyunsaturated fatty acids and their degradation products in food are also described. Chapter 2 describes the development of a gas chromatography coupled to mass spectrometry (GC-MS) method to analyze the fatty acids in food. The method was applied to eggs and pork (both standard and n-3 enriched) submitted to various cooking processes and storage conditions. This study confirmed that the fat of standard eggs or pork from the Belgian market cannot be considered as a source ofn-3 PUFA.. Indeed, in this work, we measured no n-3 PUFA in meat or only around 2 % for eggs, while the fat of both enriched eggs and pork contained about 10 % of n-3 fatty acids. After storage or cooking without culinary fat no change of the fatty acid profile was observed, in standard eggs or pork. We made the same observation for n-3 enriched products, except for some cooking processes (pan-frying and oven cooking), which induced a slight but significant loss of n-3 fatty acids in n-3 enriched eggs or pork. The fairly stable behavior of the fatty acids after various cooking and storage experiments indicates that the fatty acid profile is not a good marker of lipid alteration. As a consequence, this work was oriented to the development of analytical tools to evaluate the presence of toxic aldehydes in food and feed as secondary oxidation products coming from polyunsaturated fatty acids. Chapter 3 is dedicated to the study of the oxidative stability of linseed oil, an oil rich in n-3 fatty acids. Section 3.1 describes the development of a liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) method to evaluate the aldehyde content in linseed oil. After optimisation of the extraction, derivation, detection and quantification steps, the method was validated according to guidelines from the European legislation as well as the recommendations of the AFNOR protocol NF V 03-110 (AFNOR, 2010). The evaluated parameters included specificity/selectivity, recovery, precision, accuracy, uncertainty, limits of detection and quantification, determined using the concept of accuracy profiles. When the method was applied to first pressure linseed oil stored for several days at 60 °C according to the Schaal oven test, it was shown that 4-hydroxy-2E-hexenal (4-HHE) was the most produced aldehyde. In Section 3.2, the different patterns of oxidation of linseed oil in real-time and accelerated aging assays were investigated, in order to verify the hypothesis commonly accepted for vegetal oil that one day aging at 60 °C is equivalent to one month real time aging at room temperature. Oxidation was evaluated with the peroxide value and para-anisidine value, as well as the content in conjugated dienes and aldehydes. All four indicators of oxidation showed very different kinetic behaviours at 20 and 60 °C, showing that the hypothesis (1 day at 60 °C = 1 month at 20 °C) is not valid for linseed oil. Chapter 4 describes the adaptation and the validation of a LC-MS/MS method to determine aldehydes in animal feed samples. Indeed, as animal feed is often enriched with linseed to increase the human intake of n-3 fatty acids through the consumption of food from animal origin, the study of the oxidative stability of n-3 enriched animal feed is also of interest. To achieve that goal, the LC-MS/MS method previously developed for linseed oil was adapted to include more aldehydes and was validated using the same guidelines as in section 3.1. Finally, chapter 5 presents the discussion and conclusions about the developed methods and the results obtained as well as their comparison with literature. Future prospects to improve the evaluation of and the exposure to the markers of lipid alteration in food are proposed. [less ▲]

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See detailEvaluation of hazardous chemicals in edible insects and insect-based food intended for human consumption
Poma, G; Cuykx, M; Amato, E et al

in Food and Chemical Toxicology (2017), 100

Detailed reference viewed: 33 (4 ULg)