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Geometrical CP violation in the N-Higgs-doublet modelIvanov, Igor ; in European Physical Journal C -- Particles & Fields (2013), 73 Geometrical CP violation is a particular type of spontaneous CP violation in which the vacuum expectation values have phases which are calculable, i.e. stable against the variation of the free parameters ... [more ▼] Geometrical CP violation is a particular type of spontaneous CP violation in which the vacuum expectation values have phases which are calculable, i.e. stable against the variation of the free parameters of the scalar potential. Although originally suggested within a specific version of the three-Higgs-doublet model, it is a generic phenomenon. We investigate its viability and characteristic features in models with several Higgs doublets. Our work contains both general results and illustrative examples. [less ▲] Detailed reference viewed: 15 (0 ULg) A First Measurement Look at the Deployment and Evolution of the Locator/ID Separation Protocol; ; Donnet, Benoît ![]() in Computer Communication Review (2013), 43(1), 37-43 During the last decade, we have seen the rise of discussions regarding the emergence of a Future Internet. One of the proposed approaches leverages on the separation of the identifier and the locator ... [more ▼] During the last decade, we have seen the rise of discussions regarding the emergence of a Future Internet. One of the proposed approaches leverages on the separation of the identifier and the locator roles of IP addresses, leading to the LISP (Locator/Identifier Separation Protocol) protocol, currently under development at the IETF (Internet Engineering Task Force). Up to now, researches made on LISP have been rather theoretical, i.e., based on simulations/emulations often using Internet traffic traces. There is no work in the literature attempting to assess the state of its deployment and how this has evolved in recent years. This paper aims at bridging this gap by presenting a first measurement study on the existing worldwide LISP network (lisp4.net). Early results indicate that there is a steady growth of the LISP network but also that network manageability might receive a higher priority than performance in a large scale deployment. [less ▲] Detailed reference viewed: 3 (1 ULg) Strength enhancements in cold-formed structural sections — Part II: 2 Predictive modelsRossi, Barbara ; ; in Journal of Constructional Steel Research (2013), 83 Cold-formed structural sections are manufactured at ambient temperature and hence undergo plastic deformations, which result in an increase in yield stress and a reduction in ductility. This paper begins ... [more ▼] Cold-formed structural sections are manufactured at ambient temperature and hence undergo plastic deformations, which result in an increase in yield stress and a reduction in ductility. This paper begins with a comparative study of existing models to predict this strength increase. Modifications to the existing models are then made, and an improved model is presented and statistically verified. Tensile coupon data from existing testing programs have been gathered to supplement those generated in the companion paper and used to assess the predictive models. A series of structural section types, both cold-rolled and press-braked, and a range of structural materials, including various grades of stainless steel and carbon steel, have been considered. The proposed model is shown to offer improved mean predictions of measured strength enhancements over existing approaches, is simple to use in structural calculations and is applicable to any metallic structural sections. It is envisaged that the proposed model will be incorporated in future revisions of Eurocode 3. [less ▲] Detailed reference viewed: 17 (5 ULg) Strength enhancements in cold-formed structural sections — Part I: 2 Material testing; Rossi, Barbara ; in Journal of Constructional Steel Research (2013), 83 This paper describes a material test programme carried out as part of an extensive study into the prediction of strength enhancements in cold-formed structural sections. The experiments cover a wide range ... [more ▼] This paper describes a material test programme carried out as part of an extensive study into the prediction of strength enhancements in cold-formed structural sections. The experiments cover a wide range of cross-section geometries – twelve Square Hollow Sections (SHS), five Rectangular Hollow Sections (RHS) and one Circular Hollow Section (CHS), and materials – austenitic (EN 1.4301, 1.4571 and 1.4404), ferritic (EN 1.4509 and 1.4003), duplex (EN 1.4462) and lean duplex (EN 1.4162) stainless steel and grade S355J2H carbon steel. The experimental techniques implemented, the generated data and the analysis methods employed are fully described. The results from the current test programme were combined with existing measured stress-strain data on cold-formed sections from the literature and following a consistent analysis of the combined data set, revised values for Young’s modulus E and the Ramberg-Osgood material model parameters n, n' 0.2,u and n'0.2,1.0 are recommended. A comparison between the recommended values and the codified values provided in AS/NZS 4673, SEI/ASCE-8 and EN 1993-1-4 is also presented. The test results are also used in a companion paper for developing suitable predictive models to determine the strength enhancements in cold-formed structural sections that arise during the manufacturing processes. [less ▲] Detailed reference viewed: 34 (9 ULg) Stables isotopes in the fin whale Balaenoptera physalus from the Mediterranean Sea: implications for management and conservationDas, Krishna ; Lepoint, Gilles ; Michel, Loïc et alPoster (2013, April) The fin whale (Balaenoptera physalus) is the commonest large whale species in the Mediterranean Sea, found mostly over deep, offshore waters of the western and central portion of the region. This whale is ... [more ▼] The fin whale (Balaenoptera physalus) is the commonest large whale species in the Mediterranean Sea, found mostly over deep, offshore waters of the western and central portion of the region. This whale is known to feed mainly on krill in contrast to its Atlantic counterpart, displaying a more diversified diet. δ13C and δ15N values were analysed by IR-MS (Isoprime 100) coupled to an N-C-S elemental analyser (Vario MICRO Cube, Elementar) in 113 skin biopsies from Mediterranean fin whales sampled in 2010 and 2011 during WWF campaign at sea. A lipid normalization equation was applied (adapted from Post al. 2007) because the measured C:N ratio was > 3.5. Normalized δ13C and δ15N values ranged from -20.3 to -17.3 ‰ and from 5.9 to 8.9 ‰, respectively. These values are in good agreement with those collected previously on baleen plates from Mediterranean fin whales (Bentaleb et al., 2011). A mean enrichment of 3.4 ‰ was observed between δ15N values measured in fin whale skin biopsies and Mediterranean krill (Meganyctiphanes norvegica) confirming the importance of the krill as a major food source. The narrow width of the isotopic niche of the Mediterranean fin whale (evaluated by SIBER Stable Isotope Bayesian Ellipses) compared to the Atlantic fin whale (Ryan et al. 2012) raises many concerns in the context of global changes and long-term consequences. One could expect that species displaying narrow niches would be more susceptible to ecosystem fragmentation and other anthropogenic impacts. [less ▲] Detailed reference viewed: 17 (1 ULg) Work ability assessment in a worker population : comparison and determinants of Work Ability index and Work Ability score; ; et al in BMC Public Health (2013), 13 Background Public authorities in European countries are paying increasing attention to the promotion of work ability throughout working life and the best method to monitor work ability in populations of ... [more ▼] Background Public authorities in European countries are paying increasing attention to the promotion of work ability throughout working life and the best method to monitor work ability in populations of workers is becoming a significant question. The present study aims to compare the assessment of work ability based on the use of the Work Ability Index (WAI), a 7-item questionnaire, with another one based on the use of WAI’s first item, which consists in the worker’s self-assessment of his/her current work ability level as opposed to his/her lifetime best, this single question being termed “Work Ability score” (WAS). Methods Using a database created by an occupational health service, the study intends to answer the following questions: could the assessment of work ability be based on a single-item measure and which are the variables significantly associated with self-reported work ability among those systematically recorded by the occupational physician during health examinations? A logistic regression model was used in order to estimate the probability of observing “poor” or “moderate” WAI levels depending on age, gender, body mass index, smoking status, position held, firm size and diseases reported by the worker in a population of workers aged 40 to 65 and examined between January 2006 and June 2010 (n=12389). Results The convergent validity between WAS and WAI was statistically significant (rs=0.63). In the multivariable model, age (p<0.001), reported diseases (OR=1.13, 95%CI [1.11-1.15]) and holding a position mostly characterized by physical activity (OR=1.67, 95%CI [1.49-1.87]) increased the probability of reporting moderate or poor work ability. A work position characterized by the predominance of mental activity (OR=0.71, 95%CI [0.61-0.84]) had a favourable impact on work ability. These relations were observed regardless of the work ability measurement tool used. Conclusion The convergent validity and the similarity in results between WAI and WAS observed in a large population of employed workers should thus foster the use of WAS for systematic screening of work ability. Ageing, overweight, decline in health status, holding a mostly physical job and working in a large-sized firm increase the risk of presenting moderate or poor work ability. [less ▲] Detailed reference viewed: 13 (2 ULg) La fonction exponentielle : premières propriétésBair, Jacques ; Henry, Valérie ![]() in Tangente Sup (2013), 69 Nous passons en revue différentes façons d'introduire des fonctions exponentielles et logarithmes, montrant notamment comment procédait Euler en exploitant des infinitésimaux. Detailed reference viewed: 11 (0 ULg) Templated TiO2 mesoporous films used as highly efficient photoelectrode in Dye-Sensitized Solar CellsDewalque, Jennifer ![]() Doctoral thesis (2013) The present thesis is dedicated to the synthesis and characterization of the TiO2 semiconductor layer used as photoelectrode in dye-sensitized solar cells (DSSCs), with the aim to improve their ... [more ▼] The present thesis is dedicated to the synthesis and characterization of the TiO2 semiconductor layer used as photoelectrode in dye-sensitized solar cells (DSSCs), with the aim to improve their photovoltaic efficiencies. DSSCs have been reported by O’Regan and Grätzel in the early nineties as a very promising alternative to conventional silicon devices. Main benefits of these cells are their low cost and their mild manufacturing process. In most of the specific literature, DSSCs are made of TiO2 films prepared by doctor-blade or screen-printing of anatase nanoparticles paste. However due to the random organization of the nanoparticles, pore accessibility by the dye and electrolyte could be incomplete and some anatase crystallites could be not connected impeding electron transfer. The strategy adopted to improve the films properties and thus PV efficiencies involves a surfactant-assisted process allowing the preparation of highly porous layers with well-ordered and accessibles pores as well as improved crystallites connectivity. The main goal of this work is to increase the film surface area and perfectly control the mesostructure in terms of thickness, pore size, pore organization and pore accessibility in order to maximize the adsorption of active dye and the electrolyte infiltration inside the porous network. Special attention was paid to the tuning of the experimental settings such as the relative humidity conditions, the withdrawal speed and the choices of substrate and surfactant. Moreover, for DSSCs applications, TiO2 film has to be crystallized in form of anatase. Perfect balance between high crystallinity and mesostructure preservation was studied in order to enhance the cells efficiencies. Besides, templated films challenge is to obtain thick layers. Indeed, monolayer films are only a few hundred nanometers thick. To increase the film thickness and thus the quantity of active material, a multilayer process was tuned. Special effort was paid to overcome the surface area limitation induced by the repeated thermal treatments applied during multilayer process. We propose an alternative thermal treatment in order to limit the mesostructure degradation. We also define the maximum crystal size compatible with the preservation of the mesoarchitecture initially induced by templating. Thick films up to 4 µm were prepared from this multilayer process and show excellent efficiency in combination with N-719 dye (6.1%) when compared to values reported in the literature. Such mesostructured templated films were compared in terms of photovoltaic performances with TiO2 nanoparticles films, generally used in DSSCs. In a second part, as the goal of this thesis is to improve the current nanoparticles-based DSSCs and prove the viability of the templating alternative, a comparison of the long-term stability of both technologies was performed. To our knowledge, long-term stability of templated DSSCs has never been reported at this time. However, in case of templated films, the surface area is highly improved and the negative effects of thermal stress, light soaking and UV exposure could be heightened. Due to their higher active interface, templated films are more sensitive than nanoparticles samples to UV illumination, what can be easily solved by the use of a UV filter. However, they are as stable as nanoparticles samples under visible light soaking (UV filtered) and under thermal stress. In addition, cells were characterized by electrochemical impedance spectroscopy (EIS). Templated cells show lower transfer resistance, as well as longer electron lifetime compared to nanoparticles DSSCs. Using templated films in DSSCs is therefore really promising because higher conversion efficiencies are reached without any increase in cells degradation. Finally, stability limitation encountered by DSSCs are mostly related to the use of liquid electrolytes, which can leak out the cell. Solid-state hole transporting materials are investigated in order to overcome this issue. However, in solid-state DSSCs, TiO2 films thickness is limited to a few microns allowing the adsorption of a limited amount of photoactive dye and thus leading to a poor light harvesting. Moreover, solid-state DSSCs are characterized by incomplete electrolyte filling, impeding the dye regeneration. Both limitations further lead to low photovoltaic efficiencies. Due to the surface area improvement as well as the perfect control of the pore organization and the pore size, the templating strategy was investigated to overcome light harvesting and pore filling limitations. Templated films were prepared from different structuring agents. They show an efficient electrolyte infiltration and a two times higher dye loading compared to nanoparticles layers. Corresponding photovoltaic performances in liquid-state and solid-state DSSCs have also been evaluated. While templating allows improving the liquid-state cells efficiencies, we cannot conclude for solid-state DSSCs due to device assembly issue and/or bad contacts between the electrodes and the sample holder during the I-V measurements. We hope that the achievements of this thesis brought a significant contribution to the field of DSSCs. Indeed, the templating strategy is proved to improve the liquid-sate cells efficiency. However, the assembly of solid-state devices and subsequent I-V tests have to be investigated further. Besides, new pathways are envisaged for interesting future work in both fundamental and applied research fields, such as the synthesis of templated films with hierarchical porosity or scale-up and industrialization of the templated devices. [less ▲] Detailed reference viewed: 56 (23 ULg) OARSI recommended performance-based tests to assess physical function in people with established hip and knee osteoarthritis; ; et al in Osteoarthritis and Cartilage (2013, April), 21(Supplement April 2013), Detailed reference viewed: 3 (0 ULg) Retour au travail après une longue absence...Mairiaux, Philippe ![]() E-print/Working paper (2013) Detailed reference viewed: 16 (1 ULg) The intra-articular injection of a new chitosan biomaterial prevents the progression of osteoarthritis in ACLT rabbit modelOprenyeszk, Frédéric ; ; et alin Osteoarthritis and Cartilage (2013, April), 21(Supplement April 2013), 69 Purpose To evaluate the effects of a single intra-articular injection of a new biomaterial consisting in a mix of alginate-chitosan (AC) beads and a viscous thermogelling chitosan-based (H) hydrogel on ... [more ▼] Purpose To evaluate the effects of a single intra-articular injection of a new biomaterial consisting in a mix of alginate-chitosan (AC) beads and a viscous thermogelling chitosan-based (H) hydrogel on cartilage lesion in osteoarthritis (OA) rabbit model. These effects were compared to those obtained with the intra-articular injection of either chitosan-based (H) hydrogel without the AC bead or saline solution. Methods OA was surgically induced by the transection of the anterior cruciate ligament (ACLT) in HYLA albino rabbits. One week after surgery, animals were randomly divided into 3 groups: group I (n=7): mix of AC beads and H hydrogel; group II (n=7): H hydrogel alone; group III (n=7): saline solution (control). The treatments (900 µl) were injected intra-articularly. X-rays from the right knee were performed before surgery, at the time of injection and at sacrifice. The standard radiographs were acquired in extension and scored by the Kellgren and Lawrence (K&L) scale. After 6 weeks, animals were euthanized and the right joint was dissected. The macroscopic evaluation of cartilage from femoral condyles and tibial plateaus stained with India ink was done. Histological sections stained with Safranine-O/fast green from bearing areas of each compartment were evaluated according to the OARSI histological score. Briefly, the evaluation considered: staining of the cartilage matrix (0-6), cartilage structure (0-11), chondrocyte density (0-4) and cluster formation (0-3), where 0 represented a normal situation and 24 points the maximum severity score. Blood samples were collected the day of injection and prior the sacrifice. Prostaglandin E2 (PGE2) and C-reactive protein (CRP) were measured in serum using immunoassays. Results The X-rays analysis showed a significant decrease (p <0.05) of the K&L score in group I (AC beads and H hydrogel; 1.5 ± 0.2) compared with group II (H hydrogel; 2.2 ± 0.5) and group III (saline solution; 3.0 ± 0.4). The size and the severity of the macroscopic OA cartilage lesion tended to decrease in group I compared to the other groups. The histological global score that refers to all compartments of the knee joint was significantly decreased in group I (11.0 ± 0.7) compared to group II (14.4 ± 0.6, p <0.01) and group III (14.8 ± 0.6, p <0.001). No significant variation of PGE2 and CRP serum levels were observed in each after 6 weeks follow-up whatever the treatment injected. Conclusions This study showed that a biphasic hydrogel composed by AC beads and H hydrogel prevented OA in rabbit with ACL transection. This effect was not observed with the hydrogel alone, suggesting that AC beads play a role in joint protection. The preventive effect was observed in all joint compartments indicating a global protective effect of this new viscosupplementation. [less ▲] Detailed reference viewed: 3 (1 ULg) Effects of chondroitin sulfate on the gene expression profile in IL-1β stimulated synovial fibroblast cells culturesLambert, Cécile ; ; et alin Osteoarthritis and Cartilage (2013, April), 21(Supplement April 2013), Purpose: Chondroitin sulfate (CS) is one the most used molecules in the management of OA. In this study, we performed a microarray analysis and identified a differential expression profile between control ... [more ▼] Purpose: Chondroitin sulfate (CS) is one the most used molecules in the management of OA. In this study, we performed a microarray analysis and identified a differential expression profile between control and IL-1β stimulated synovial fibroblast cells cultures. In a second step, we investigated the effects of CS on this gene expression profile. Methods: OA synovial specimens were obtained from 12 patients undergoing knee replacement. At the surgery time, the synovial membrane was dissected. Synovial fibroblast cells (SFC) were enzymatically isolated and used after four passages (P4). SFC were pre-treated 1 hour with highly purified bovine CS (200 µg/ml, Bioibérica S.A., Barcelona, Spain) before treatment with IL-1β (1 ng/ml) for 24 hours. Total RNA was extracted using the RNeasy Mini Kit. RNA purity and quality were evaluated using the Experion RNA StdSens Analysis kit (Bio-rad Laboratories). Gene expression profiling was performed using Illumina’s multi-sample format Human HT-12 BeadChip (Illumina Inc.). Differential analysis was performed with the BRB array tools software. Class comparison test between control (Ctl) and interleukin (IL)-1β conditions, Ctl and Ctl/CS and IL-1β and IL-1β/CS conditions was based on paired t-test where Ctl and IL-1β, Ctl and Ctl/CS and IL-1β and IL-1β/CS were paired for each patient. The biological relevance of up- and down-regulated genes was analyses with Ingenuity Pathways Analysis (Ingenuity® Systems). Probes with a p-value below 0.001 were chosen and classified as up- or down-regulated ones. Results: 3308 genes were identified as differentially expressed genes between Ctl and IL-1β conditions. We observed a differential profile of expression of major pathways involved in OA pathogenesis. The key identified pathways were related to inflammation, complement cascade, angiogenesis, cartilage catabolism and anabolism and Wnt signaling. In the inflammatory network, the most upregulated cytokines were IL-8 and IL-6 with a fold change of 156.25 and 58.8 respectively. We also identified several chemokines, enzymes and metallothioneins (MTs). Complement factor B (CFB) and complement component 3 (C3) are two factors upregulated in the inflammatory complement cascade. We also identified some genes implicated in the angiogenesis pathway. The most upregulated was Stanniocalcin 1 (STC1) with a fold change of 9.09. The differential expression of intermediates involved in both cartilage anabolism and catabolism was revealed by the IL-1β stimulation, showing an imbalance in favour of catabolism. MMP-3 was largely upregulated (fold change of 62.5). Wnt 5A and low density lipoprotein receptor-related protein (LRP8) were significantly upregulated while frizzled homolog 2 (FZD2) and dickkopf homolog 3 (DKK3) were downregulated in the Wnt signaling pathway. We next performed a class comparison test between Ctl and Ctl/CS in one hand and IL-1β and IL-1β/CS on the other hand. 660 genes were identified as differentially expressed between Ctl and Ctl/CS conditions while 241 genes were identified between IL-1β and IL-1β/CS. Among them, our attention was focused on two genes upregulated in the presence of CS: lysyl oxidase-like 4 (LOXL4) and claudin 11 (CDLN11), two genes that negatively regulate cell invasion. Conclusions: We here evidenced in synovial fibroblast cells the modulation of gene expression following IL-1β stimulation. We also demonstrated the modulatory effects of CS on gene expression and isolated several CS-modulated genes of interest such as LOXL4 and CDLN11, which could constitute new mechanisms of action of the molecule and contribute to explain the symptomatic efficacy of CS in the treatment of OA. [less ▲] Detailed reference viewed: 1 (0 ULg) Effects of chondroitin sulfate on the gene expression profile in the inflamed synovial membraneLambert, Cécile ; ; et alin Osteoarthritis and Cartilage (2013, April), 21(Supplement April 2013), Purpose: The aim of the present work was to identify the differentially expressed genes between the inflammatory (I) and normal/reactive (N/R) synovial areas using a unique ex vivo culture model. In a ... [more ▼] Purpose: The aim of the present work was to identify the differentially expressed genes between the inflammatory (I) and normal/reactive (N/R) synovial areas using a unique ex vivo culture model. In a second step, we investigated the genetic modulatory effects of chondroitin sulfate (CS) in this model. Methods: Synovial cells (SC) were isolated from OA synovial specimens obtained from 12 patients undergoing knee replacement. The inflammatory status of the synovial membrane was characterized according to macroscopic criteria. At the surgery time, the synovial membrane was dissected and biopsies from N/R and I areas cultured separately for a period of 7 days in the absence or in the presence of highly purified bovine CS (200 µg/ml, Bioibérica S.A., Barcelona, Spain). Total RNA was extracted using the RNeasy Mini Kit. RNA purity and quality were evaluated using the Experion RNA StdSens Analysis kit (Bio-rad Laboratories). Gene expression profiling was performed using Illumina’s multi-sample format Human HT-12 BeadChip (Illumina Inc.). Differential analysis was performed with the BRB array tools software. Class Comparison test between N/R and I conditions, N/R and N/R-CS conditions and I and I-CS conditions was based on paired t-test where N/R and I, N/R and N/R-CS and I and I-CS were paired for each patient. The biological relevance of up- and down-regulated genes was analyses with Ingenuity Pathways Analysis (Ingenuity® Systems). Results: From among 47000 probes, 18253 were filtered out. Probes with a p-value below than 0.005 were chosen and classified as up- or down-regulated ones. By this way, 465 differentially expressed genes between N/R and I areas were identified. Many inflammatory mediators appear differentially expressed. The interferon alpha-inductible protein 6 (IFI6) was the most up-regulated. We also identified the hydroxysteroid (11-beta) dehydrogenase 1 (HSD11B1), the cathepsin K (CTSK), the chemokine (C-X-C motif) ligand 1 (CXCL1) and the EBV-induced G-protein coupled receptor 2 (EBI2). The differential expression of intermediates involved in angiogenesis pathway was also revealed between N/R and I areas. Among them, R-spondin-3 (RSPO3), the secreted phopshoprotein 1 (SPP1) and aquaporin 9 (AQP9) were up-regulated whereas ADAMTS1 was down-regulated. Finally, in the Wnt signaling, RSPO3 was up-regulated unlike dickkopf homolog 3 (DKK3) which was in turn down-regulated. We next performed a class comparison test between N/R and N/R-CS in one hand and between I and I-CS the other hand. 489 genes were identified as differentially expressed genes between N/R and N/R-CS conditions while 219 genes were identified between I and I-CS conditions. In this latter, our attention was focused on the down-regulated genes. Among them, we identified a number implicated in angiogenesis and cell migration pathways. Thus, the endothelial cell-specific molecule-1 (ESM1), the Transmembrane-4-L-six-family-1 (TM4SF1), the 5’-Ectonucleotidase (NT5E) and the growth arrest-specific gene 6 (GAS6) were down-regulated by CS. Conclusions: Our work demonstrates the differential gene expression profile between paired non inflammatory and normal/reactive areas of synovial membrane as well as the modulatory effects of CS on gene expression in the inflammatory areas, especially regarding genes involved in both angiogenesis and cell migration. [less ▲] Detailed reference viewed: 12 (0 ULg) SOUTHERN BLUES NIGHT , Heerlen (NL) 17e ed. , 15 mars 2013Sacré, Robert ![]() E-print/Working paper (2013) C.R. de la 17e Southern Blues Night , le 15 mars 2013 à Heerlen ( Nl) + photos ; Khalif Wailin' Walter , Mud Morganfield, Heritage Blues Orchestra, James Harman, Enrico Crivellaro, Gene Taylor, .... Detailed reference viewed: 3 (0 ULg) Investigation of potential new targets for the diagnosis and/or the treatment of osteoarthritisLambert, Cécile ; ; et alin Osteoarthritis and Cartilage (2013, April), 21(Supplement April 2013), Purpose: Synovial inflammation plays a key role in the pathophysiology process of osteoarthritis (OA). We have previously compared the gene expression pattern of synovial cells isolated from inflammatory ... [more ▼] Purpose: Synovial inflammation plays a key role in the pathophysiology process of osteoarthritis (OA). We have previously compared the gene expression pattern of synovial cells isolated from inflammatory (I) or normal/reactive (N/R) areas of a synovial membrane harvested from the same OA patient. We identified a large number of mediators belonging to key pathways involved in OA pathogenesis. The aim of this study was to validate different potential new targets for the diagnosis and/or the treatment OA. Methods: Synovial cells (SC) were isolated from synovial specimens obtained from OA patients undergoing knee replacement. The inflammatory status of the synovial membrane was characterized according to macroscopic criteria. The biopsies from N/R and I areas were cultured separately for a period of 7 days. Microarray gene expression profiling between N/R and I areas was performed. The biological relevance of up- and down-regulated genes was analyzed with Ingenuity Pathways Analysis. Western blot and immunohistochemistry confirmed the identified genes most differentially expressed in the key pathways. The production of the triggering receptor expressed on myeloid cells-1 (TREM1), the alarmin S100 calcium binding protein A9 (S100A9), the wingless-type MMTV integration site family, member 5A (Wnt-5A) and the stanniocalcin 1 (STC1) were evaluated by Western blot. S100A9, hyaluronan synthase-1 (HAS1) and STC1 expression and localization were investigated by immunohistochemistry. Results: 896 genes differentially expressed in N/R and I areas were identified. The key pathways were related to inflammation, cartilage metabolism, Wnt signaling and angiogenesis. In the inflammatory gene pattern, TREM1 and S100A9 were strongly upregulated. We validated the production of these proteins in OA synovial biopsies by Western blot. TREM1 and S100A9 were increased in I compared to N/R synovial cells culture. S100A9 was observed in the perivascular area and in sublining cells in I synovial biopsies, but not in N/R biopsies. An increased staining was also observed in the intima lining layer of I when compared to N/R biopsies. The most upregulated anabolism enzyme in I synovial biopsies was HAS1. Using immunohistochemistry, we observed in I areas an increase of the HAS1-positive cells mainly in the intima lining. We also studied the protein production of Wnt-5A, the most upregulated intermediate of Wnt signaling pathway. The protein level was increased in I compared to N/R areas. Finally, in the angiogenesis pathway, one the most u-regulated gene was STC1. A significant increase of STC1 production was observed in I areas compared to N/R areas by Western blot. This result was also supported by the immunohistochemical analysis. In I area, the staining for STC1 was more intense in perivascular and sublining cells. Conclusions: Synovial membrane inflammation is a key target for OA treatments. In this work, we have identified proteins involved in the synovitis pathways like angiogenesis, cells infiltration and matrix remodeling. These proteins could be targeted by drugs and used as companion biomarkers for evaluating their efficacy. Although qualitative, our results could also yield to the identification of markers of the disease. This investigation has to be further pursued. [less ▲] Detailed reference viewed: 19 (0 ULg) ON THE MODELING OF ADHESIVE CONTACT AND STICTION FAILURE IN MICRO-SWITCHESWu, Ling ; Noels, Ludovic ; Golinval, Jean-Claude ![]() in Ouisse (FEMTO-ST – ENSMM, France); Deü (CNAM, FR), J.F. (Eds.) MEDYNA 2013 Proceedings (2013, April) Undesirable stiction, which results from contact between surfaces, is a major failure mode in micro-switches. Indeed the adhesive forces can become so important that the two surfaces remain permanently ... [more ▼] Undesirable stiction, which results from contact between surfaces, is a major failure mode in micro-switches. Indeed the adhesive forces can become so important that the two surfaces remain permanently glued, limiting the life-time of the MEMS. This is especially true when contact happens between surfaces where elasto-plastic asperities deform permanently until the surfaces reach plastic accommodation, increasing the surface forces. To predict this behavior, a micro adhesive-contact model is developed, which accounts for the surfaces topography evolutions during elasto-plastic contacts. This model can be used at a higher scale to study the MEMS behavior, and thus its life-time. For illustration purpose, an electrostatic-structural analysis is performed on a micro-switch. [less ▲] Detailed reference viewed: 14 (3 ULg) Substrate Marking by an Invasive Ladybeetle: Seasonal Changes in Hydrocarbon Composition and Behavioral ResponsesDurieux, Delphine ; Fassotte, Bérénice ; et alin PLoS ONE (2013), 8(4), The multicolored Asian ladybeetle, Harmonia axyridis (Pallas), aggregates inside dwellings during the winter to survive the cold. Recent published reports have highlighted that overwintering individuals ... [more ▼] The multicolored Asian ladybeetle, Harmonia axyridis (Pallas), aggregates inside dwellings during the winter to survive the cold. Recent published reports have highlighted that overwintering individuals use hydrocarbon markings deposited on surfaces by conspecifics to orient toward aggregation sites. In the current study, monthly GC-MS analyses revealed seasonal modifications in the chemical profile of substrate markings deposited by moving individuals. The markings of overwintering ladybeetles contained larger proportions of heptacosadiene, nonacosadiene, hentriacontadienes, and methyl-nonacosanes, along with a lower proportion of heptacosene and nonacosene. This finding suggests the importance of the unsaturated and/or branched hydrocarbons in the H. axyridis aggregation process. Subsequently, we conducted behavioral assays to test whether (1) there is seasonal variation in the behavioral response of H. axyridis individuals toward substrate markings deposited by conspecifics in the same physiological state and (2) the observed behavioral modification is due to a change in ladybeetle sensitivity and/or a change in the chemical composition of the substrate marking. The results indicate that overwintering individuals exhibit a stronger ‘‘following’’ response toward conspecific substrate markings. This behavior is linked to both the physiological state of ladybeetles and the specific chemical profile of the marking biomolecules deposited under overwintering conditions. [less ▲] Detailed reference viewed: 25 (9 ULg) Conception des pseudo-articulations à col paraboiqueDebongnie, Jean-François ![]() in Agiuzoul, Mohammed (Ed.) 11ème Congrès de Mécanique - Actes du Congrès (2013, April) The elastic behaviour of parabolic notch hinges submitted to the classical sollicitations of a beam is studied. For each sollicitation, an analytical solution is given first. Its asymptotic expression for ... [more ▼] The elastic behaviour of parabolic notch hinges submitted to the classical sollicitations of a beam is studied. For each sollicitation, an analytical solution is given first. Its asymptotic expression for very narrow hinges is then obtained and finally,, an approximate factor relating the exact solution to the asymptotic one is proposed. [less ▲] Detailed reference viewed: 9 (1 ULg) Trente ans de lutte contre le racisme en Belgique : bilan et perspectivesJamin, Jérôme ![]() in Revue Nouvelle (2013), année 68(numéro 4), Detailed reference viewed: 3 (1 ULg) Extensions of Superalgebras of Krichever-Novikov typeKreusch, Marie ![]() Poster (2013, April) Detailed reference viewed: 26 (10 ULg) |
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