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See detailExergy analysis applied to performance of buildings in Europe
Sartor, Kevin ULg; Dewallef, Pierre ULg

in Energy and Buildings (2017), 148

Energy performance of buildings generally assesses the energy consumption of buildings such as heating, domestic heat water, ventilation systems, etc. However, this approach is based on the first law of ... [more ▼]

Energy performance of buildings generally assesses the energy consumption of buildings such as heating, domestic heat water, ventilation systems, etc. However, this approach is based on the first law of thermodynamics and considers only the quantity of energy used without considering its ‘quality’ and leads to a lack of information about the energy conversion processes. This is particularly true in the new low-energy buildings where sometimes high temperatures sources are used to meet low-temperature needs. The exergy analysis of a system, based on first and second thermodynamic laws, can be used to overcome this. In this work, it is proposed to compare the energy and the exergy consumption and the related CO2 emissions of several kinds of buildings to determine the best systems in terms of energy and exergy needs. The energy demand calculations are performed using the official software available in Belgium and some assumptions are implemented to consider the exergy approach. As exergy calculations require a reference state, some different climatic conditions are also investigated. Finally, some conclusions are discussed to rank the sources of energy and their related exergy losses. [less ▲]

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See detailMolecular Tools for the Detection and Quantification of Toxigenic Cyanobacteria
Kurmayer, Rainer; Sivonen, Kaarina; Wilmotte, Annick ULg et al

Book published by John Wiley and sons LTD - This edition first published 2017 (2017)

A guide to state-of-the-art molecular tools for monitoring and managing the toxigenicity of cyanobacteria Runaway climate change has made the monitoring and management of toxigenic organisms in the ... [more ▼]

A guide to state-of-the-art molecular tools for monitoring and managing the toxigenicity of cyanobacteria Runaway climate change has made the monitoring and management of toxigenic organisms in the world’s bodies of water more urgent than ever. In order to influence public policy regarding the detection and quantification of those organisms, it is incumbent upon scientists to clearly demonstrate to policy makers the increase of toxigenic cyanobacteria and the threats they pose. As molecular methods can handle many samples in short time, they are the most reliable, cost-effective tools currently available for tracking cyanotoxicity worldwide. This volume arms scientists with the tools they need to track toxigenicity in surface waters and food supplies and, hopefully, to develop new techniques for managing the spread of toxic cyanobacteria. This book offers the first comprehensive treatment of molecular tools for monitoring cyanotoxicity. Growing out of the findings of the landmark European Cooperation in Science and Technology Cyanobacteria project (CYANOCOST), it provides detailed, practical coverage of the full array of available molecular tools and protocols, from water sampling, nucleic acid extraction, and downstream analysis—including PCR and qPCR based methods—to genotyping (DGGE), diagnostic microarrays, and community characterization using next-gen sequencing techniques. [less ▲]

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See detailBioinformatic Processing of Amplicon Sequencing Datasets
Sweetlove, Maxime; Obbels, Dagmar; Verleyen, Elie et al

in Kurmayer, Rainer; Sivonen, Kaarina; Wilmotte, Annick (Eds.) et al Molecular Tools for the Detection and Quantification of Toxigenic Cyanobacteria (2017)

Amplicon sequencing can be a very powerful approach for detecting toxic cyanobacteria or any other kind of microorganism during monitoring programs. However, owing to the huge size of next-generation ... [more ▼]

Amplicon sequencing can be a very powerful approach for detecting toxic cyanobacteria or any other kind of microorganism during monitoring programs. However, owing to the huge size of next-generation sequencing (NGS) datasets (up to several Gb), there is an obvious need for semi-automatic data processing and statistical analysis, as well as visualization of the patterns found. Importantly, raw NGS data contain errors, some of which are easily detected (e.g. too short or low-quality reads), while others remain hidden even after the most stringent quality controls (e.g. chimeras, contaminations, reads with large insertions or deletions, referred to as “indels”). As a consequence, NGS data need to be interpreted with caution, and bioinformatics analysis implementing poor error identification can easily lead to erroneous conclusions. Hence, a crucial step in the analysis of NGS data is the detection and removal of as many erroneous reads as possible. Moreover, bioinformatics involve additional preprocessing steps, including demultiplexing (i.e. grouping reads to samples according to the barcode sequence), deleting non-biological tags together with the adaptors and primer sequences, and removing chimeric sequences. In addition, the bioinformatics pipelines enable the quality-filtered sequences to be clustered into biologically relevant operational taxonomic units (OTUs), which form the basis of the statistical analysis, including the calculation of alpha- and beta-diversity. [less ▲]

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See detailTaxonomic Identification of Cyanobacteria by a Polyphasic Approach
Wilmotte, Annick ULg; Laughinghouse, Dail Haywood IV; Capelli, Camilla et al

in Kurmayer, Rainer; Sivonen, Kaarina; Wilmotte, Annick (Eds.) et al Molecular Tools for the Detection and Quantification of Toxigenic Cyanobacteria (2017)

In this chapter, we shall discuss the criteria and methods to be adopted for the taxonomic identification and classification of cyanobacteria. This includes a brief introduction of the two Codes of ... [more ▼]

In this chapter, we shall discuss the criteria and methods to be adopted for the taxonomic identification and classification of cyanobacteria. This includes a brief introduction of the two Codes of Nomenclature ruling in parallel on the valid naming of these organisms. We shall then present the major steps important for cyanobacterial taxa identification and their nomenclature. These include: 1) determination of morphology by light microscopy; 2) genetic characterization by single and/or multilocus sequence typing; 3) the assignment of the organism to a taxonomic entity (genus, species, eco- and/or genotype within a species) by reference to, and phylogenetic analysis of, cyanobacterial nucleotide sequences available in public repositories. Ideally, these methods should be accompanied by the determination of other relevant properties (ultrastructural, physiological, biochemical, and ecological characteristics) that may help to define/redefine and circumscribe the cyanobacterial taxon under study. [less ▲]

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See detailIsolation, Purification, and Cultivation of Toxigenic Cyanobacteria
Haande, Sigrid; Jasser, Iwona; Gugger, Muriel et al

in Kurmayer, Rainer; Sivonen, Kaarina; Wilmotte, Annick (Eds.) et al Molecular Tools for the Detection and Quantification of Toxigenic Cyanobacteria (2017)

This chapter summarizes the most commonly used methods for the isolation, purification, and cultivation of toxic cyanobacteria. The aim is to give general advice on how to isolate and maintain clonal ... [more ▼]

This chapter summarizes the most commonly used methods for the isolation, purification, and cultivation of toxic cyanobacteria. The aim is to give general advice on how to isolate and maintain clonal cyanobacterial cultures in order to use them in genetic studies. The traditional methods for the isolation of cyanobacteria into culture are well established and described and there are several excellent reviews with detailed information on culturing techniques [less ▲]

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See detailDNA (Diagnostic) and cDNA Microarray
Rantala-Yilinen, Anne; Sivonen, Kaarina; Wilmotte, Annick ULg

in Kurmayer, Rainer; Sivonen, Kaarina; Wilmotte, Annick (Eds.) et al Molecular Tools for the Detection and Quantification of Toxigenic Cyanobacteria (2017)

Diagnostic microarrays have been used to study cyanobacterial diversity in environmental samples. They usually include sequences (probes) for only one or a few genes allowing a number of genera/species to ... [more ▼]

Diagnostic microarrays have been used to study cyanobacterial diversity in environmental samples. They usually include sequences (probes) for only one or a few genes allowing a number of genera/species to be detected. In contrast, cDNA microarrays are used for screening genome-wide changes in gene expression and include probes for all or most genes in one strain . The purpose of this sub-chapter is to introduce the principle of a diagnostic microarray (= DNA chip) that uses a ligation detection reaction (LDR) and universal microarray to simultaneously detect and identify all potential microcystin and nodularin producers present in a sample. This is especially useful when monitoring environmental samples that can contain many cyanobacterial genera, including toxin-producing strains. [less ▲]

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See detailAssessing quality of life using structural equation modeling
Dardenne, Nadia ULg; Pétré, Benoît ULg; Husson, Eddy ULg et al

Poster (2017, August)

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See detailSimilarity of the Jovian satellite footprints: spots multiplicity and dynamics
Bonfond, Bertrand ULg; Grodent, Denis ULg; Badman, S. V. et al

in Icarus (2017), 292(2017), 208217

In the magnetospheres of Jupiter and Saturn, the intense interaction of the satellites Io, Europa, Ganymede and Enceladus with their surrounding plasma environment leaves a signature in the aurora of the ... [more ▼]

In the magnetospheres of Jupiter and Saturn, the intense interaction of the satellites Io, Europa, Ganymede and Enceladus with their surrounding plasma environment leaves a signature in the aurora of the planet. Called satellite footprints, these auroral features appear either as a single spot (Europa and Enceladus) or as multiple spots (Io and Ganymede). Moreover, they can be followed by extended trailing tails in the case of Io and Europa, while no tail has been reported for Ganymede and Enceladus, yet. Here we show that all Jovian footprints can be made of several spots. Furthermore, the footprints all experience brightness variations on timescale of 2-3 minutes. We also demonstrate that the satellite location relative to the plasma sheet is not the only driver for the footprint brightness, but that the plasma environment and the magnetic field strength also play a role. These new findings demonstrate that the Europa and Ganymede footprints are very similar to the Io footprint. As a consequence, the processes expected to take place at Io, such as the bi-directional electron acceleration by Alfvén waves or the partial reflection of these waves on plasma density gradients, can most likely be extended to the other footprints, suggesting that they are indeed universal processes. [less ▲]

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See detailInfluence of urban patterns on flooding
Bruwier, Martin ULg; Mustafa, Ahmed; Aliaga, Daniel et al

in E-proceedings of the 37th IAHR World Congress (2017, August)

The goal of this paper is to identify the respective influence of different characteristics of urban patterns on urban flooding. A set of 2,290 alternate urban patterns was generated randomly using an ... [more ▼]

The goal of this paper is to identify the respective influence of different characteristics of urban patterns on urban flooding. A set of 2,290 alternate urban patterns was generated randomly using an urban generator tool providing the geometry of buildings and their relative location to the ground, over a square area of 1 km². Steady 2-D hydraulic computations were performed for these 2,290 different urban patterns with identical hydraulic boundary conditions. The computational time was reduced by using an anisotropic porosity model. This model uses relatively coarse computational cells; but preserves information from the detailed topographic data through the use of porosity parameters. Based on the computed maps of waterdepths for the 2,290 urban patterns, a sensitivity analysis based on a multiple linear regression was performed to outline the most influential urban characteristics. [less ▲]

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See detailVoice use among music theory teachers
Schiller, Isabel ULg; Morsomme, Dominique ULg; Sfez, Lou et al

Conference (2017, August)

In Belgium, there is a particular group of music teachers referred to as music theory teachers. Working at music schools, they convey theoretical knowledge and practical musical skills to groups of ... [more ▼]

In Belgium, there is a particular group of music teachers referred to as music theory teachers. Working at music schools, they convey theoretical knowledge and practical musical skills to groups of individuals who learn music during their free-time. Even though music theory teachers use both speaking and singing voice intensively at work, little is known about their voice use profiles. This study investigated the vocal loading among French-speaking music theory teachers. Objectives were (1) to describe their professional and extra-professional vocal loading, (2) to determine the relationship between vocal loading and background noise level and (3) to investigate whether objectively measured vocal loading is reflected in music theory teachers’ auto-evaluation of their voice. Using voice dosimetry, 13 music theory teachers were monitored for one workweek. Parameters analysed were F0, voice sound pressure level (SPL), phonation time, vocal loading index (VLI) and background noise SPL. At the end of each monitoring day, subjects self-assessed their voice use by means of visual analogue scales. Results revealed (1) significantly higher vocal loading in the professional context than in the extra-professional context, (2) significant positive correlations between background noise level and the parameters F0, voice SPL and phonation time and (3) significant correlations between the VLI and auto-evaluation data (e.g. voice quality and vocal fatigue). These results highlight that teaching music theory is a profession with high vocal demands. At work, music theory teachers are exposed to high background noise, which seems to influence their voice use and may potentially contribute to the development of voice problems among this population. Visual analogue scales provide a promising tool to subjectively investigate vocal loading among music theory teachers. [less ▲]

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See detailEarly eukaryotes: insights from microanalyses of proterozoic microfossils
Cornet, Yohan ULg; Beghin, Jérémie ULg; Kabamba Baludikay, Blaise ULg et al

in Goldschmidt Abstracts, 2017 (2017, August)

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See detailAn efficient algorithm to decide periodicity of b-recognisable sets using MSDF convention
Boigelot, Bernard ULg; Mainz, Isabelle ULg; Marsault, Victor ULg et al

in Leibniz International Proceedings in Informatics (2017, August), 80

Given an integer base b>1, a set of integers is represented in base b by a language over {0,1,...,b-1}. The set is said to be b-recognisable if its representation is a regular language. It is known that ... [more ▼]

Given an integer base b>1, a set of integers is represented in base b by a language over {0,1,...,b-1}. The set is said to be b-recognisable if its representation is a regular language. It is known that eventually periodic sets are b-recognisable in every base b, and Cobham's theorem implies the converse: no other set is b-recognisable in every base b. We are interested in deciding whether a $b$-recognisable set of integers (given as a finite automaton) is eventually periodic. Honkala showed that this problem is decidable in 1986 and recent developments give efficient decision algorithms. However, they only work when the integers are written with the least significant digit first. In this work, we consider the natural order of digits (Most Significant Digit First) and give a quasi-linear algorithm to solve the problem in this case. [less ▲]

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See detailAs teorias semióticas face à fotografia
Dondero, Maria Giulia ULg

in Calavari, Suzi; Rodrigues, Angelica; Rocha, Nildicéia (Eds.) Proceedings VI SELIN – NOVAS PRÁTICAS EM PESQUISA SOBRE A LINGUAGEM: ROMPENDO FRONTEIRAS (2017, August)

Dans ce texte, je me propose de revenir sur les travaux en sémiotique qui ont marqué les études du champ interdisciplinaire de la photographie et de les poursuivre; l’objectif principal est de fournir ... [more ▼]

Dans ce texte, je me propose de revenir sur les travaux en sémiotique qui ont marqué les études du champ interdisciplinaire de la photographie et de les poursuivre; l’objectif principal est de fournir quelques pistes pour étudier, d’un point de vue sémiotique, l’image photographique en tant qu’objet pris dans des pratiques d’usage et d’interprétation diversifiées au sein des statuts sociaux de l’art, de la science, de la religion. [less ▲]

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See detailNETPerfTrace – Predicting Internet Path Dynamics and Performance with Machine Learning
Wassermann, Sarah ULg; Casas, Pedro; Cuvelier, Thibaut ULg et al

in Proceedings of Big-DAMA ’17 (2017, August)

We study the problem of predicting Internet path changes and path performance using traceroute measurements and machine learning models. Path changes are frequently linked to path inflation and ... [more ▼]

We study the problem of predicting Internet path changes and path performance using traceroute measurements and machine learning models. Path changes are frequently linked to path inflation and performance degradation, therefore the relevance of the problem. We introduce NETPerfTrace, an Internet Path Tracking system to forecast path changes and path latency variations. By relying on decision trees and using empirical distribution-based input features, we show that NETPerfTrace can predict (i) the remaining life time of a path before it actually changes and (ii) the number of path changes in a certain time period with relatively high accuracy. Through extensive evaluation, we demonstrate that NETPerfTrace highly outperforms DTRACK, a previous system with the same prediction targets. NETPerfTrace also offers path performance forecasting capabilities. In particular, our tool can predict path latency metrics, providing a system which can not only predict path changes, but also forecast their impact in terms of performance variations. We release NETPerfTrace as open software to the networking community, as well as all evaluation datasets. [less ▲]

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See detailGiving an account of fictional discourse. Meinongian logics versus modal logics
Leclercq, Bruno ULg

Conference (2017, August)

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See detailRegiospecific radiolabelling of Nanofitin on Ni Magnetic Beads with [18F]FBEM and in vivo PET studies
Dammicco, Sylvestre ULg; Goux, Marine; Lemaire, Christian ULg et al

in Nuclear Medicine & Biology (2017)

Introduction: Nanofitins are low molecular weight, single chain and cysteine-free protein scaffolds able to selectively bind a defined biological target. They derive from Sac7d bacterial protein family ... [more ▼]

Introduction: Nanofitins are low molecular weight, single chain and cysteine-free protein scaffolds able to selectively bind a defined biological target. They derive from Sac7d bacterial protein family and are highly stable over a wide range of pH (0-13) and temperature (Tm ~80°C). Their extreme stability, low cost of production and high tolerability for chemical coupling make Nanofitins a very interesting alternative to antibodies and their fragments. Here, a hexahistidine tagged model Nanofitin (H4) directed against hen egg white lysozyme was radiolabelled and injected in mice to provide a baseline biodistribution and pharmacokinetic profiles to support future Nanofitin development programs. Method: A single cysteine residue has been genetically inserted in a model Nanofitin and its regioselective radiolabelling has been performed with 4-[18F]fluorobenzamido-N-ethylamino-maleimide ([18F]FBEM). The synthesis of [18F]FBEM has been completely implemented on a radiosynthesis unit (FastLab) including HPLC purification and formulation. Coupling with the [18F]FBEM has been achieved on a solid support (Ni magnetic beads) allowing rapid purification at room temperature without organic solvent. PET-MRI studies on C57BL/6 mice were conducted after injection of [18F]FBEM-Cys-H4 in order to access the biodistribution of this Nanofitin model. Results: Radiochemical yield (decay corrected) of 54±7% (n=4) was obtained after optimization for coupling the [18F]FBEM to Nanofitin. Pharmacokinetics results of [18F]FBEM-Cys-H4 revealed a fast clearance through the liver and the kidneys. Conclusion: An efficient new method on Ni magnetic beads was developed to radiolabelled his-tagged biomolecules with [18F]FBEM. This procedure was applied on a Nanofitin model Cys-H4 and biodistribution kinetic studies were achieved to evaluate the potential use of Nanofitin for diagnostic imaging. Fast clearance indicates that Nanofitins represent very interesting tools for diagnostic imaging. [less ▲]

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See detailForeseeing New Control Challenges in Electricity Prosumer Communities
Olivier, Frédéric ULg; Marulli, Daniele; Ernst, Damien ULg et al

in Proc. of the 10th Bulk Power Systems Dynamics and Control Symposium – IREP’2017 (2017, August)

This paper is dedicated to electricity prosumer communities, which are groups of people producing, sharing and consuming electricity locally. This paper focuses on building a rigorous mathematical ... [more ▼]

This paper is dedicated to electricity prosumer communities, which are groups of people producing, sharing and consuming electricity locally. This paper focuses on building a rigorous mathematical framework in order to formalise sequen- tial decision making problems that may soon be encountered within electricity prosumer communities. After introducing our formalism, we propose a set of optimisation problems reflecting several types of theoretically optimal behaviours for energy exchanges between prosumers. We then discuss the advantages and disadvantages of centralised and decentralised schemes and provide illustrations of decision making strategies, allowing a prosumer community to generate more distributed electricity (compared to commonly applied strategies) by mitigating over- voltages over a low-voltage feeder. We finally investigate how to design distributed control schemes that may contribute reaching (at least partially) the objectives of the community, by resort in to machine learning techniques to extract, from centralised solution(s), decision making patterns to be applied locally. First empirical results show that, even after a post-processing phase so that it satisfies physical constraints, the learning approach still performs better than predetermined strategies targeting safety first, then cost minimisation. [less ▲]

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See detailPrevalence of voice disorders among teachers at the beginning of their career
Schiller, Isabel ULg

Poster (2017, August)

Due to the high vocal demands associated with their profession, teachers face an increased risk of developing voice disorders. Research suggests that up to 50 % of experienced teachers are affected. Even ... [more ▼]

Due to the high vocal demands associated with their profession, teachers face an increased risk of developing voice disorders. Research suggests that up to 50 % of experienced teachers are affected. Even student teachers, whose vocal load is still relatively low, report voice problems with a frequency of 20 % (Simberg, Laine, Sala, & Rönnemaa, 2000). Little is known about the prevalence of voice disorders among teachers at the very beginning of their career. In Germany, teachers must complete a two-year teaching practice as part of their professional training after they graduate from university, consisting of attending specialized courses and giving lessons. The aim of this symptom-based study was to determine the prevalence of voice problems among this population. A self-administered questionnaire was answered by 73 German teachers in teaching practice. The prevalence of a voice problem was defined based on the presence of two symptoms that persisted for a minimum of two weeks. Results revealed that 37 % of teachers had voice problems during their teaching practice. Among the most frequent symptoms were hoarseness, an urge to clear one’s throat and problems speaking at a loud voice. Considering that the participants had not even begun teaching full-time, the prevalence of reported voice problems is worrisome. It could be associated with their lacking experience in how to use their voice effectively. The findings highlight the necessity of early intervention programs focusing on vocal hygiene and effective voice use in classroom situations. [less ▲]

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