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See detailIMISCOE Research Network, Training and Research Inventory 2015
Mescoli, Elsa ULg

Report (in press)

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See detailShort communication: Evaluation of the microbiota of kefir samples using metagenetic analysis targeting the 16S and 26S ribosomal DNA fragments
Korsak Koulagenko, Nicolas ULg; Taminiau, Bernard ULg; Leclercq, Mathilde et al

in Journal of Dairy Science (in press)

Milk kefir is produced by fermenting milk in the presence of kefir grains. This beverage has several benefits for human health. The aim of this experiment was to analyze 5 kefir grains (and their products ... [more ▼]

Milk kefir is produced by fermenting milk in the presence of kefir grains. This beverage has several benefits for human health. The aim of this experiment was to analyze 5 kefir grains (and their products) using a targeted metagenetic approach. Of the 5 kefir grains analyzed, 1 was purchased in a supermarket, 2 were provided by the Ministry of Agriculture (Namur, Belgium), and 2 were provided by individuals. The metagenetic approach targeted the V1-V3 fragment of the 16S ribosomal (r)DNA for the grains and the resulting beverages at 2 levels of grain incorporation (5 and 10%) to identify the bacterial species population. In contrast, the 26S rDNA pyrosequencing was performed only on kefir grains with the aim of assessing the yeast populations. In parallel, pH measurements were performed on the kefir obtained from the kefir grains using 2 incorporation rates. Regarding the bacterial population, 16S pyrosequencing revealed the presence of 20 main bacterial species, with a dominance of the following: Lactobacillus kefiranofaciens, Lactococcus lactis ssp. cremoris, Gluconobacter frateurii, Lactobacillus kefiri, Acetobacter orientalis, and Acetobacter lovaniensis. An important difference was noticed between the kefir samples: kefir grain purchased from a supermarket (sample E) harbored a much higher proportion of several operational taxonomic units of Lactococcus lactis and Leuconostoc mesenteroides. This sample of grain was macroscopically different from the others in terms of size, apparent cohesion of the grains, structure, and texture, probably associated with a lower level of Lactobacillus kefiranofaciens. The kefir (at an incorporation rate of 5%) produced from this sample of grain was characterized by a lower pH value (4.5) than the others. The other 4 samples of kefir (5%) had pH values above 5. Comparing the kefir grain and the kefir, an increase in the population of Gluconobacter in grain sample B was observed. This was also the case for Acetobacter orientalis in sample D. In relation to 26S pyrosequencing, our study revealed the presence of 3 main yeast species: Naumovozymaspp., Kluyveromyces marxianus, and Kazachastania khefir. For Naumovozyma, further studies are needed to assess the isolation of new species. In conclusion, this study has proved that it is possible to establish the patterns of bacterial and yeast composition of kefir and kefir grain. This was only achieved with the use of high-throughput sequencing techniques. [less ▲]

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See detailStéphane Mallarmé (1842-1898)
Durand, Pascal ULg

in Sapiro, Gisèle (Ed.) Dictionnaire international Bourdieu (in press)

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See detailUPLC®-MS/MS, an analytical tool for the accurate and rapid quantification of phytoestrogen metabolites in milk
Daems, Frédéric ULg; Jasselette, Christophe; Romnee, Jean-Michel et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (in press)

The term ‘phytoestrogen’ is used to define a wide variety of nonsteroidal compounds that occur naturally in many plants. When they are absorbed by cows, these polyphenolic compounds undergo ... [more ▼]

The term ‘phytoestrogen’ is used to define a wide variety of nonsteroidal compounds that occur naturally in many plants. When they are absorbed by cows, these polyphenolic compounds undergo biotransformation by specific bacteria present in the gastrointestinal tract. The resulting metabolites are absorbed in the organism and some are excreted in milk. The impact of these compounds on human health divides opinion. Some scientists believe that they might have adverse health effects. Others believe the opposite, especially with regard to their microbial metabolites, such as equol. Whatever one's view on the subject, scientists need accurate, sensitive and rapid analytical methods in order to continue the research on clarifying the phytoestrogen issue. Cow's milk is an animal product that is common in the human diet, and it is therefore important to evaluate its content of phytoestrogen metabolites. In order to study the human intake of equol via commercial milk, an analytical method was developed and validated following EMA/CVMP/VICH/463202/2009 guidelines. Enzymatic hydrolysis was used to release the equol. It was then extracted using double liquid/liquid extraction and analyzed using UPLC®-MS/MS, with an analysis runtime of only 5 min. This analytical method produced a linear calibration curve with a high correlation coefficient (R2≥0.996) between 5 and 1,000 ng mL−1. Good intra- and inter-day precision (RSDs≤5.3% and ≤5.2%, respectively) and accuracy (bias≤8.6%) were achieved. The recovery rate differed slightly among the different types of milk, ranging between 60.6±1.09% and 82.3±5.21%. Good method repeatability was observed (RSDs<15%). There was neither a matrix effect nor a carry-over effect, and the sample extracts were stable during storage for at least 7 days at −21°C and 5°C. In order to apply the proposed method and obtain an initial estimate of equol concentration in cow’s milk in Belgium, 44 samples of various brands found in several supermarkets and 5 raw milk samples from Walloon farms were analyzed. Equol was found in all the samples analyzed, with a concentration ranging from 10 to 50 ng mL−1 for conventional milk and from 70 to 130 ng mL−1 for organic milk. These results were compared with other European studies and the same trend was observed. The results obtained show the specificity, sensitivity and precision of this method for analyzing oestrogenic metabolite-equol in cow’s milk. This study should be extended to include a greater number of samples and be implemented over a longer period to see if there is a correlation between livestock practice and/or sample origins with the equol content in milk. The addition of other phytoestrogen compounds with this method, using UPLC®-MS/MS technology, could also lead to more reliable monitoring of these compounds in dairy production. [less ▲]

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See detailX-linked acrogigantism (X-LAG) syndrome : Clinical Profile and Therapeutic responses
Beckers, Albert ULg; Lodish, MB; Trivellin, G et al

in Endocrine-Related Cancer (in press)

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See detailRisques d'inégalités liés à certaines caractéristiques des manuels contemporains de langues modernes
Simons, Germain ULg; Delbrassine, Daniel ULg; Van Hoof, Florence ULg

in Recherche en Education (2016), 25

Cet article épingle huit caractéristiques de trois manuels de langues contemporains (anglais, allemand, espagnol), qui présentent des risques d'inégalités pour les apprenants. Chacune des caractéristiques ... [more ▼]

Cet article épingle huit caractéristiques de trois manuels de langues contemporains (anglais, allemand, espagnol), qui présentent des risques d'inégalités pour les apprenants. Chacune des caractéristiques est illustrée par des exemples issus des trois manuels. [less ▲]

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See detailBrentano, la théorie des catégories et la définition de la substance
Boccaccini, Federico ULg

in Etudes Philosophiques (2016)

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See detailIntroduction
Englebert, Jérôme ULg; Follet, Valérie

in Englebert, Jérôme; Follet, Valérie (Eds.) Adaptation : Essai collectif à partir des paradigmes éthologiques et évolutionnistes (2016)

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See detailConclusion : Anormalité ou anomalie ?
Englebert, Jérôme ULg; Follet, Valérie

in Englebert, Jérôme; Follet, Valérie (Eds.) Adaptation : Essai collectif à partir des paradigmes éthologiques et évolutionnistes (2016)

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See detailPressure-impulse diagram of a beam under developing non-linear membrane action under
Hamra, Lotfi ULg; Demonceau, Jean-François ULg; Denoël, Vincent ULg

in International Journal of Impact Engineering (2016)

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See detailAdaptation : Essai collectif à partir des paradigmes éthologiques et évolutionnistes
Englebert, Jérôme ULg; follet, Valérie

Book published by Hermann (2016)

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See detailDépasser le paradoxe évolutionniste de la schizophrénie : psychopathologie éthologique et adaptation perpétuelle
Englebert, Jérôme ULg

in Englebert, Jérôme; Follet, Valérie (Eds.) Adaptation : Essai collectif à partir des paradigmes éthologiques et évolutionnistes (2016)

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See detailProvenance of Ichthyosaura alpestris (Caudata: Salamandridae) introductions to France and New Zealand assessed by mitochondrial DNA analysis
Arntzen, Jan W.; King, Tania M.; Denoël, Mathieu ULg et al

in The Herpetological Journal (2016), 26

The last century has seen an unparalleled movement of species around the planet as a direct result of human activity, which has been a major contributor to the biodiversity crisis. Amphibians represent a ... [more ▼]

The last century has seen an unparalleled movement of species around the planet as a direct result of human activity, which has been a major contributor to the biodiversity crisis. Amphibians represent a particularly vulnerable group, exacerbated by the devastating effects of chytrid fungi. We report the malicious translocation and establishment of the alpine newt (Ichthyosaura alpestris) to its virtual antipode in North Island of New Zealand. We use network analysis of mitochondrial DNA haplotypes to identify the original source population as I. a. apuana from Tuscany, Italy. Additionally, a population in southern France, presumed to be introduced, is identified as I. a. alpestris from western Europe. However, the presence of two differentiated haplotypes suggests a mixed origin. This type of analysis is made possible by the recent availability of a phylogenetic analysis of the species throughout its natural range. We discuss the particulars of both introductions. [less ▲]

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See detailReconnaissance et parentification: des mots qui vont si bien ensemble?
Haxhe, Stéphanie ULg

Conference given outside the academic context (2015)

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See detailCahiers d'Etudes Germaniques : Lecteurs/spectateurs d'Alexander Kluge
Cormann, Grégory ULg; Hamers, Jérémy ULg; Letawe, Céline ULg

Book published by Université de Provence (2015)

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See detailLe pouvoir des sentiments : Kluge, Adorno, Ferenczi
Hamers, Jérémy ULg; Cormann, Grégory ULg

in Cahiers d'Etudes Germaniques (2015)

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See detailDerivation and Application of Sensitivities to Assess Transient Voltage Sags caused by Rotor Swings
Weckesser, Tilman; Johannsson, Hjortur; Ostergaard, Jacob et al

in International Journal of Electrical Power & Energy Systems (2015), 72

The paper introduces an approach to investigate voltage sags, which are caused by large generator rotor swings following a transient disturbance. Therefore, the method exploits sensitivities derived from ... [more ▼]

The paper introduces an approach to investigate voltage sags, which are caused by large generator rotor swings following a transient disturbance. Therefore, the method exploits sensitivities derived from the algebraic network equations. These provide information on the impact of a generator on the voltage magnitude at a load bus and the e ffect of load variation on the generator's power injection. It is shown that these sensitivities give valuable information to identify critical generator-load pairs and locations for applying preventive control measures. [less ▲]

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