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See detailChitin-glucan complex production by Komagataella pastoris: downstream optimization and product characterization
Farinha, Inês; Duarte, Paulo; Pimentel, Ana et al

in Carbohydrate Polymers (in press)

Purified chitin-glucan complex (CGCpure) was extracted from Komagataella pastoris biomass using a hot alkaline treatment, followed by neutralization and repeated washing with deionised water. The co ... [more ▼]

Purified chitin-glucan complex (CGCpure) was extracted from Komagataella pastoris biomass using a hot alkaline treatment, followed by neutralization and repeated washing with deionised water. The co-polymer thus obtained had a glucan:chitin molar ratio of 75:25 and low protein and inorganic salts contents (3.0 and 0.9 wt%, respectively). CGCpure had an average molecular weight of 4.9 × 105 Da with a polydispersity index of 1.7, and a crystallinity index of 50%. Solid-state NMR provided structural insight at the co-polymer. X-ray diffraction and FTIR analysis suggest that CGCpure has b-chitin in its structure. CGCpure presented an endothermic decomposition peak at 315 oC, assigned to the degradation of the saccharide structures. This study revealed that K. pastoris CGC has properties similar to other chitinous biopolymers and may represent an attractive alternative to crustacean chitin derived-products, being a reliable raw material for the development of new/improved pharmaceutical, cosmetic or food products. [less ▲]

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See detailA radio-map of the colliding winds in the very massive binary system HD93129A
Benaglia, Paula; Marcote, Benito; Moldon, Javier et al

in Astronomy and Astrophysics (in press)

Radio observations are an effective tool to discover particle acceleration regions in colliding-wind binaries, through detection of synchrotron radiation; these regions are natural laboratories for the ... [more ▼]

Radio observations are an effective tool to discover particle acceleration regions in colliding-wind binaries, through detection of synchrotron radiation; these regions are natural laboratories for the study of relativistic particles.Wind-collision region (WCR) models can reproduce the radio continuum spectra of massive binaries that contain both thermal and non-thermal radio emission; however, key constraints for models come from high-resolution imaging. Only five WCRs have been resolved to date at radio frequencies at milliarcsec (mas) angular scales. The source HD93129A, prototype of the very few known O2 I stars, is a romising target for study: recently, a second massive, early-type star about 50 mas away was discovered, and a non-thermal radio source detected in the region. Preliminary long-baseline array data suggest that a significant fraction of the radio emission from the system comes from a putative WCR. We sought evidence that HD93129A is a massive binary system with colliding stellar winds that produce non-thermal radiation, through spatially resolved images of the radio emitting regions. Methods.We completed observations with the Australian Long Baseline Array (LBA) to resolve the system at mas angular resolutions and reduced archival Australia Telescope Compact Array (ATCA) data to derive the total radio emission. We also compiled optical astrometric data of the system in a homogeneous way. We reduced historical Hubble Space Telescope data and obtained absolute and relative astrometry with milliarcsec accuracy. The astrometric analysis leads us to conclude that the two stars in HD93129A form a gravitationally bound system. The LBA data reveal an extended arc-shaped non-thermal source between the two stars, indicative of a WCR. The wind momentum-rate ratio of the two stellar winds is estimated. The ATCA data show a point source with a change in flux level between 2003-4 and 2008-9, that is modeled with a non-thermal power-law spectrum with spectral indices of - 1:03 +/- 0:09 and -1:21 +/- 0:03 respectively. The mass-loss rates derived from the deduced thermal radio emission and from the characteristics of the WCR are consistent with estimates derived by other authors. [less ▲]

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See detailArticle "Hippocrate"
Ricciardetto, Antonio ULg

in Zawieja, Philippe (Ed.) Dictionnaire de la fatigue (in press)

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See detailArticle "Galien"
Ricciardetto, Antonio ULg

in Zawieja, Philippe (Ed.) Dictionnaire de la fatigue (in press)

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See detailKin recognition and inbreeding avoidance in a butterfly
Fischer; Karl, Isabell; Heuskin, Stéphanie ULg et al

in Ethology (in press)

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See detailSe faire « manger par l’autre ». Stratégie féminines d’affirmation de soi en contexte public.
Mescoli, Elsa ULg

in Etrangers au Maghreb. Maghrébins à l’étranger : politiques publiques à l’épreuve de la société civile : XIXe-XXIe siècles. Actes du colloque. (in press)

Cette communication vise à étudier la participation des femmes migrantes marocaines à la vie sociale du « pays d’accueil » ; participation qui se réalise grâce aux activités de la vie quotidienne. En ... [more ▼]

Cette communication vise à étudier la participation des femmes migrantes marocaines à la vie sociale du « pays d’accueil » ; participation qui se réalise grâce aux activités de la vie quotidienne. En particulier, la performance publique des pratiques culinaires, observée dans une ville de la province de Milan, permet aux femmes considérées d’exercer une agency à l’intérieur des espaces institutionnellement concédés à l’expression des migrants. D’un côté, la consommation des « recettes de l’Autre » de la part de la population majoritaire détermine une incorporation de la diversité afin de la contrôler. De l’autre côté, la préparation de ces recettes consent aux migrantes d’exprimer leur subjectivité (individuelle et collective) de façon active et en présentant des aspects qui vont au-delà de la culture alimentaire. Une participation sociale fondée sur le partage de nourriture donne la possibilité aux femmes d’assumer un rôle actif dans la communauté locale, tout en affirmant ses propres spécificités. Des sujets tels que la liberté religieuse sont abordés, et certains droits peuvent être réclamés par des individus qui ont ainsi acquis une position politiquement importante au sein de la société locale. [less ▲]

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See detailCountries of origin as organisers of emigration: Moroccans and Turks in Belgium.
Gsir, Sonia ULg; Mandin, Jérémy ULg; Mescoli, Elsa ULg

in Fargues, P.; Weinar, A.; Di Bartolomeo, A. (Eds.) et al From Home to Home? How countries of origin impact immigrant integration outcomes, volume 2 (empirical findings), (in press)

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See detailHow to manage an isolated elevated PTH?
Souberbielle, Jean-Claude; CAVALIER, Etienne ULg; Cormier, Catherine

in Annales d'Endocrinologie (in press)

The aim of this article is to discuss the diagnostic approach of an increased serum PTH concentration in a normocalcemic, normophosphatemicpatient. Detection of this biological presentation is frequent in ... [more ▼]

The aim of this article is to discuss the diagnostic approach of an increased serum PTH concentration in a normocalcemic, normophosphatemicpatient. Detection of this biological presentation is frequent in routine practice all the more that PTH reference values established in vitamin Dreplete subjects with a normal renal function are used by the clinical laboratories. The first step in this diagnostic approach will be to rule out acause of secondary hyperparathyroidism (SHPT). Among these, the most frequent are vitamin D deficiency, very low calcium intake, impairedrenal function, malabsorptions, drugs interfering with calcium/bone metabolism, such as lithium salts and antiresorptive osteoporosis therapies,hypercalciuria due to a renal calcium leak. If no cause of SHPT are evidenced, the diagnosis of normocalcemic primary hyperparathyroidism(PHPT) should be considered. A calcium load test is a very useful tool for this diagnosis if it shows that serum PTH is not sufficiently decreasedwhen calcemia rises frankly above the upper normal limit. In a normocalcemic patient with hypercalciuria and a high serum PTH concentration,a thiazide challenge test may help to differentiate SHPT due to a renal calcium leak from normocalcemic PHPT. Beyond the discussion of thisdiagnostic [less ▲]

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See detailIMISCOE Research Network, Training and Research Inventory 2015
Mescoli, Elsa ULg

Report (in press)

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See detailStéphane Mallarmé (1842-1898)
Durand, Pascal ULg

in Sapiro, Gisèle (Ed.) Dictionnaire international Bourdieu (in press)

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See detailAbelian bordered factors and periodicity
Charlier, Emilie ULg; Harju, Tero; Puzynina, Svetlana et al

in European Journal of Combinatorics (in press)

A finite word u is said to be bordered if u has a proper prefix which is also a suffix of u, and unbordered otherwise. Ehrenfeucht and Silberger proved that an infinite word is purely periodic if and only ... [more ▼]

A finite word u is said to be bordered if u has a proper prefix which is also a suffix of u, and unbordered otherwise. Ehrenfeucht and Silberger proved that an infinite word is purely periodic if and only if it contains only finitely many unbordered factors. We are interested in abelian and weak abelian analogues of this result; namely, we investigate the following question(s): Let w be an infinite word such that all sufficiently long factors are (weakly) abelian bordered; is w (weakly) abelian periodic? In the process we answer a question of Avgustinovich et al. concerning the abelian critical factorization theorem. [less ▲]

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See detailUPLC®-MS/MS, an analytical tool for the accurate and rapid quantification of phytoestrogen metabolites in milk
Daems, Frédéric ULg; Jasselette, Christophe; Romnee, Jean-Michel et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (in press)

The term ‘phytoestrogen’ is used to define a wide variety of nonsteroidal compounds that occur naturally in many plants. When they are absorbed by cows, these polyphenolic compounds undergo ... [more ▼]

The term ‘phytoestrogen’ is used to define a wide variety of nonsteroidal compounds that occur naturally in many plants. When they are absorbed by cows, these polyphenolic compounds undergo biotransformation by specific bacteria present in the gastrointestinal tract. The resulting metabolites are absorbed in the organism and some are excreted in milk. The impact of these compounds on human health divides opinion. Some scientists believe that they might have adverse health effects. Others believe the opposite, especially with regard to their microbial metabolites, such as equol. Whatever one's view on the subject, scientists need accurate, sensitive and rapid analytical methods in order to continue the research on clarifying the phytoestrogen issue. Cow's milk is an animal product that is common in the human diet, and it is therefore important to evaluate its content of phytoestrogen metabolites. In order to study the human intake of equol via commercial milk, an analytical method was developed and validated following EMA/CVMP/VICH/463202/2009 guidelines. Enzymatic hydrolysis was used to release the equol. It was then extracted using double liquid/liquid extraction and analyzed using UPLC®-MS/MS, with an analysis runtime of only 5 min. This analytical method produced a linear calibration curve with a high correlation coefficient (R2≥0.996) between 5 and 1,000 ng mL−1. Good intra- and inter-day precision (RSDs≤5.3% and ≤5.2%, respectively) and accuracy (bias≤8.6%) were achieved. The recovery rate differed slightly among the different types of milk, ranging between 60.6±1.09% and 82.3±5.21%. Good method repeatability was observed (RSDs<15%). There was neither a matrix effect nor a carry-over effect, and the sample extracts were stable during storage for at least 7 days at −21°C and 5°C. In order to apply the proposed method and obtain an initial estimate of equol concentration in cow’s milk in Belgium, 44 samples of various brands found in several supermarkets and 5 raw milk samples from Walloon farms were analyzed. Equol was found in all the samples analyzed, with a concentration ranging from 10 to 50 ng mL−1 for conventional milk and from 70 to 130 ng mL−1 for organic milk. These results were compared with other European studies and the same trend was observed. The results obtained show the specificity, sensitivity and precision of this method for analyzing oestrogenic metabolite-equol in cow’s milk. This study should be extended to include a greater number of samples and be implemented over a longer period to see if there is a correlation between livestock practice and/or sample origins with the equol content in milk. The addition of other phytoestrogen compounds with this method, using UPLC®-MS/MS technology, could also lead to more reliable monitoring of these compounds in dairy production. [less ▲]

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See detailThe Perceived Value of Passive Animal Health Surveillance: The Case of Highly Pathogenic Avian Influenza in Vietnam
Delabouglise, Alexis; Antoine-Moussiaux, Nicolas ULg; Phan, T.D et al

in Zoonoses and Public Health (in press)

Economic evaluations are critical for the assessment of the efficiency and sustain- ability of animal health surveillance systems and the improvement of their effi- ciency. Methods identifying and ... [more ▼]

Economic evaluations are critical for the assessment of the efficiency and sustain- ability of animal health surveillance systems and the improvement of their effi- ciency. Methods identifying and quantifying costs and benefits incurred by public and private actors of passive surveillance systems (i.e. actors of veterinary author- ities and private actors who may report clinical signs) are needed. This study pre- sents the evaluation of perceived costs and benefits of highly pathogenic avian influenza (HPAI) passive surveillance in Vietnam. Surveys based on participatory epidemiology methods were conducted in three provinces in Vietnam to collect data on costs and benefits resulting from the reporting of HPAI suspicions to vet- erinary authorities. A quantitative tool based on stated preference methods and participatory techniques was developed and applied to assess the non-monetary costs and benefits. The study showed that poultry farmers are facing several options regarding the management of HPAI suspicions, besides reporting the fol- lowing: treatment, sale or destruction of animals. The option of reporting was associated with uncertain outcome and transaction costs. Besides, actors antici- pated the release of health information to cause a drop of markets prices. This cost was relevant at all levels, including farmers, veterinary authorities and private actors of the upstream sector (feed, chicks and medicine supply). One benefit associated with passive surveillance was the intervention of public services to clean farms and the environment to limit the disease spread. Private actors of the poultry sector valued information on HPAI suspicions (perceived as a non-mon- etary benefit) which was mainly obtained from other private actors and media. [less ▲]

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See detailQuantify ing cereal - reaping microwear on flint tools: an experimental approach
Linton, Jimmy ULg; Monna, Fabrice; Martineau, Rémi et al

in Archaeometry (in press)

From the earliest Neolithic in the Near East to the last Chalcolithic cultures in Western Europe, certain flint tools have been used as sickles to harvest cereals. Such harvesting tools can be identified ... [more ▼]

From the earliest Neolithic in the Near East to the last Chalcolithic cultures in Western Europe, certain flint tools have been used as sickles to harvest cereals. Such harvesting tools can be identified through use-wear analyses, because cutting herbaceous plants produces specific wear-traces on the working edge of flint blades. The aim of this work is to explore harvesting-driven microwear variability and, more particularly, intensity of use as a governing factor. To achieve this objective, an experiment was designed consisting in the production of flint replicas to be used as harvesting tools, in various controlled conditions. A simple, cost-effective method of quantifying wear-traces by meas uring polish extent is developed, requiring only classical use-wear observation equipment. Polish extent shows monotonic correlation with the amount of work, expressed either in terms of time or as the quantity of cereals harvested. Polish extent measurement is therefore proposed as a reliable and powerful descriptor of use intensity. [less ▲]

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See detailPolitique agricole commune. Analyse transversale de la conditionnalité environnementale
Habran, Maxime ULg

Book published by Bruylant (in press)

Pierre angulaire de la construction européenne, la politique agricole commune (PAC) est à l’origine mise en place dans le but d’assurer l’autosuffisance alimentaire de la Communauté européenne. L’objectif ... [more ▼]

Pierre angulaire de la construction européenne, la politique agricole commune (PAC) est à l’origine mise en place dans le but d’assurer l’autosuffisance alimentaire de la Communauté européenne. L’objectif, rapidement atteint, laisse dans son sillage des stocks et excédents considérables ainsi que des dépenses communautaires importantes. Le mode de production agricole basé sur un modèle productiviste montre rapidement ses limites, obligeant les États membres à une réorientation de la PAC. Cette politique sera modifiée et réformée à de nombreuses reprises. La reconnaissance de la protection de l’environnement comme politique transversale contraint les États membres à l’adapter. D’une politique productiviste, elle devient attentive aux considérations environnementales, intégrant ces dernières dans chacune des réformes, allant même jusqu’à lier l’octroi de paiement au respect de règles essentiellement environnementales. Cet ouvrage présente la manière dont s’articule la relation entre les deux politiques autour de la notion de conditionnalité environnementale. À travers une étude minutieuse de chaque réforme de la PAC, une analyse de transversalité permet de saisir les enjeux et les motivations de l’intégration environnementale. [less ▲]

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