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See detailNew carbohydrate-active enzymes identified by screening two metagenomic libraries derived from the soil of a winter wheat field
Stroobants, Aurore ULg; Portetelle, Daniel ULg; Vandenbol, Micheline ULg

in Journal of Applied Microbiology (2014)

Aims Soils are rich, diversified environments where β-glucosidases abound because of their importance in organic matter degradation. The aim of this work was to discover new β-glucosidases by constructing ... [more ▼]

Aims Soils are rich, diversified environments where β-glucosidases abound because of their importance in organic matter degradation. The aim of this work was to discover new β-glucosidases by constructing two metagenomic DNA libraries from soil samples collected in winter and spring from a field of winter wheat. Methods and Results Both libraries were screened on esculin-supplemented medium so as to isolate candidates showing β-glucosidase activity. Candidate analysis revealed seven putative β-glycosidases and two putative glycosyltransferases, displaying 25 to 82% identity to known enzymes. The putative β-glycosidases belong to families GH1, GH3 and GH20 and the two putative glycosyltransferases, probably, to new families. In characterization tests performed on bacteria in suspension or spread on agar plates, some candidates appeared to hydrolyse several natural and synthetic substrates. These tests also highlighted interesting industrial characteristics, such as the activity of four β-glycosidases under alkaline conditions and the esculin-hydrolysing activity of a β-glucosidase candidate in the presence of glucose. Conclusions Seven putative β-glycosidases and two putative glycosyltransferases were found by functional screening of two metagenomic DNA libraries derived from agricultural soil. Significance and Impact of the Study This study has identified β-glycosidases and putative glycosyltransferases that have or may have interesting industrial characteristics. [less ▲]

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See detailJamming Signal Immunity Tests on GSM-R Communications Compared to EMC Basic Standards
Beauvois, Véronique ULg; Fontana, Michele; Canavero, Flavio et al

Conference (2014, July 29)

This paper presents the work being done in SECRET project (European Program FP7/2007-2013 under grant agreement number “285136”) to strengthen the European rail network against the potential jamming of ... [more ▼]

This paper presents the work being done in SECRET project (European Program FP7/2007-2013 under grant agreement number “285136”) to strengthen the European rail network against the potential jamming of the railway communications. Standardized immunity tests, currently conducted on communication equipment in the presence of electromagnetic (EM) interferences are presented. It then presents the tests carried out in SECRET to assess the resistance of railway communication systems face the signals generated by telecommunication jammers. [less ▲]

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See detailPhotographier le travail, représenter le futur. Iconographie du chantier des Halles à Paris
Dondero, Maria Giulia ULg

in Communication et Langages (2014), 180

This article investigates the semiotic strategies of visual representation in the urban space, as exemplified by the renovation project of the Forum des Halles in Paris. The corpus of images, taken from ... [more ▼]

This article investigates the semiotic strategies of visual representation in the urban space, as exemplified by the renovation project of the Forum des Halles in Paris. The corpus of images, taken from the brochure of the construction site and from the website www.parisleshalles.fr, includes photographic documentation and illustrations of the future buildings. The analysis of the visual enunciation marks out the relative simulacra of the producers and consumers of these images, and a semiotic study of the modes of representation of the buildings (virtualisation, actualisation, realisation, etc.) helps understanding how the present and the future are articulated in the iconography of a construction work in progress. [less ▲]

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See detailEuropean Union Public Diplomacy through the EEAS: the cases of Argentina, Brazil and Mexico
Azpíroz Manero, María Luisa ULg

Conference (2014, July 25)

Public diplomacy is a promising activity for international political actors to promote a positive image abroad. It also provides opportunities for dialogue, understanding and mutual benefit when ... [more ▼]

Public diplomacy is a promising activity for international political actors to promote a positive image abroad. It also provides opportunities for dialogue, understanding and mutual benefit when communicating and addressing challenges. Within the European Union (EU), the Lisbon Treaty and the European External Action Service (EEAS) have given a new strength to foreign policy. The EEAS, launched in December 2010, is the EU's main institution for public diplomacy. Argentina, Brazil and Mexico are relevant Latin American partners for the EU because of their size, population and political and economic potency in the region. This paper aims to answer the following questions: What is the context of EU relations with Argentina, Brazil and Mexico during the period of study (2011-2013)? What have been the EU's foreign policy and strategies towards these countries? How have public diplomacy and the work of the EEAS in these countries supported the objectives and strategies? This research includes information gained through the consultation of publications, official documents and interviews with personnel from the EEAS, both in Brussels and in the EU delegations in Latin America. [less ▲]

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See detailStreamable Laguerre-Voronoi Tessellation Model for Tomographic Images
Leblanc, Christophe ULg; Nguyen, Van Dung ULg; Wan, Fangyi et al

Conference (2014, July 25)

Introduction. Nowadays, the interest in foam materials is growing in several engineering fields [1]. Foams can exhibit a nonlinear mechanical behavior [2], which is highly depen- dent on their ... [more ▼]

Introduction. Nowadays, the interest in foam materials is growing in several engineering fields [1]. Foams can exhibit a nonlinear mechanical behavior [2], which is highly depen- dent on their microstructure [3]. Thus designing foams with specific mechanical properties can be very challenging. The present contribution is part of the ARC-Bridging project [4], whose objective is to predict the mechanical behavior of complex microstructured mate- rials via numerical simulations. A possible classification of foam models into two groups is: random models and deterministic models [5]. The random models frequently require statistical estimations of their parameters [6], whereas the deterministic models generally require numerically expensive image analyse. Indeed, classical analysis steps involve a distance tranform, a watershed and, optionally, a h-minima transform [5, p. 22], which can be computationally demanding [7, 8, 9]. Contribution. In the present Laguerre–Voronoi tessellation model, the image analysis part neither involes the watershed transform, nor the h-minima transform. Instead, fol- lowing the original idea of A.M. Lopez-Reina et E. Béchet [10], these two transforms are respectively replaced by a Hessian-based removal of spurious extrema and a clustering of the remaining maxima. This substitution allows the processing of large 3D-images by slices, i.e. “streaming”. The only limitation is enforced by the distance transform: the “feature” voxel of a given voxel should belong to the same slice. For foam images, this condition is fulfilled as long as the slice’s thickness is larger than the maximal foam cell’s size. Conclusion and perspectives. The aim of this contribution is to provide an efficient tessellation model for tomographic images of foams. From input tomographic images, this model provides a geometry model which will be used as an input for finite element simulations under the ARC-Briding project [4]. Simulation results will be compared with experimental measures. [less ▲]

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See detailSeasonal variability of meiofauna, especially harpacticoid copepods, in Posidonia oceanica macrophytodetritus accumulations
Mascart, Thibaud ULg; Lepoint, Gilles ULg; Deschoemaeker, Silke et al

in Journal of Sea Research (2014)

The overall aim of this study was (1) to assess the diversity and density of meiofauna taxa, especially harpacticoid copepod species, present within accumulated seagrass macrophytodetritus on unvegetated ... [more ▼]

The overall aim of this study was (1) to assess the diversity and density of meiofauna taxa, especially harpacticoid copepod species, present within accumulated seagrass macrophytodetritus on unvegetated sand patches and (2) to elucidate the community structure of detritus-associated harpacticoid copepods in relation to natural temporal variability of physico-chemical characteristics of accumulations. This was investigated in a Posidonia oceanica (L.) Delile seagrass ecosystem in the northwest Mediterranean Sea (Bay of Calvi, Corsica, 42°35’N, 8°43’E) using a triplicate macrophytodetritus core field sampling in two contrasting sites over the four seasons of 2011. Meiofauna higher taxa consisted of 50% Copepoda, which 87% belonged to the Harpacticoida order. Nematoda was the second most abundant taxa. The copepod community displayed a wide variety of morphologically similar and ecologically different species (i.e. mesopsammic, phytal, phytal-swimmers, planktonic and parasitic). The harpacticoid copepod community followed a strong seasonal pattern with highest abundances and species diversity in May-August, revealing a link with the leaf litter epiphyte primary production cycle. Aside from the important role in sheltering, housing and feeding potential of macrophytodetritus, a harpacticoid community BEST analysis demonstrated a positive correlation with habitat complexity and a negative correlation with water movements and P. oceanica leaf litter accumulation. [less ▲]

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See detailQuelle histoire pour les forêts d'Afrique centrale ?
Morin-Rivat, Julie ULg

Scientific conference (2014, July 24)

Conférence grand public sur les indicateurs de perturbations climatiques et anthropiques dans les forêts d'Afrique centrale.

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See detailGEOMETRICAL VALIDITY OF HIGH-ORDER PYRAMIDAL FINITE ELEMENTS
Johnen, Amaury ULg; Geuzaine, Christophe ULg

Conference (2014, July 24)

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See detailSur les quantifications équivariantes en supergéométrie de contact
Nibirantiza, Aboubacar ULg

Doctoral thesis (2014)

Detailed reference viewed: 29 (11 ULg)
See detailBrokers and intermediaries in environmental governance, Southern Sri Lanka
Rosillon, Caroline ULg

Conference (2014, July 23)

Since the end of the civil war in 2009, rural Southern Sri Lanka is facing growing tensions between fast economic development and the conservation of biodiversity, which contributes to the notoriety of ... [more ▼]

Since the end of the civil war in 2009, rural Southern Sri Lanka is facing growing tensions between fast economic development and the conservation of biodiversity, which contributes to the notoriety of the island. In order to preserve the green areas of the country, both the government and international institutions have made great efforts to implement projects combining biodiversity protection and poverty reduction as their objectives. Within that context, this paper focuses on a specific category of stakeholders, emerging from the implementation of the aforementioned projects: a wide range of intermediaries between local communities and the State, or international institutions. Based on an ethnographic study of two protected areas in Southern Sri Lanka, the paper will examine the role of those intermediaries in the implementation of environmental governance models and ideologies in local arenas. I will also analyze the complex relationships between those brokers and other categories of individuals or groups within the community: people living in protected areas, traditional figures of power, social and political elites, etc. Using fieldwork illustrations, I will stress how the emergence of brokers and intermediaries in the context of nature conservation may lead to the reorganization of social and political relationships at the local scale. [less ▲]

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See detailRécits de vie d’adolescents. Humeur dépressive et figures parentales
Poudat, Céline; Boulard, Aurore ULg

in 4ème Congrès Mondial de Linguistique Française (2014, July 22)

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See detailDetection of differential item functioning using the lasso approach
Magis, David ULg; Tuerlinckx, Francis; De Boeck, Paul

Conference (2014, July 22)

The purpose of this talk is to present a novel approach to detect differential item functioning (DIF) among dichotomously scored items. Unlike standard DIF methods that perform an item-by-item analysis ... [more ▼]

The purpose of this talk is to present a novel approach to detect differential item functioning (DIF) among dichotomously scored items. Unlike standard DIF methods that perform an item-by-item analysis, we consider a logistic regression model including item-group interaction (i.e. DIF) effects of all items simultaneously. The method is based on penalized maximum likelihood estimation of a model with a lasso penalty on all possible DIF parameters. Optimal penalty parameter selection is investigated through several known information criteria (such as AIC and BIC) as well as a newly developed weighted alternative. A simulation study was conducted to compare the global performance of the suggested “lasso DIF” method to the logistic regression and Mantel-Haenszel methods, and to evaluate the different optimal penalty parameter selection methods. It is concluded that for small samples the lasso DIF approach globally outperforms the logistic regression method, and also the Mantel-Haenszel method, especially in the presence of item impact, while it yields similar results with larger samples. [less ▲]

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See detailThe performance of mobile devices' inertial measurement unit for the detection of cattle's behaviors on pasture
Andriamandroso, Andriamasinoro ULg; Dumont, Benjamin ULg; Lebeau, Frédéric ULg et al

Conference (2014, July 21)

Over the past decade, the Precision Livestock Farming (PLF) concept has taken a considerable place in the development of accurate methods for a better management of farm animals, as Precision Agriculture ... [more ▼]

Over the past decade, the Precision Livestock Farming (PLF) concept has taken a considerable place in the development of accurate methods for a better management of farm animals, as Precision Agriculture has done for crop production. Mass consumption mobile devices have nowadays the possibility to record accurately user movements with their Inertial Measurement Unit (IMU). We used iPhone 4S to detect accurately cattle behaviors such as grazing and ruminating with the aim of performing a precision grazing management on the near future. Results showed accuracies ranging between 84% and 100% when detecting these two major behaviors by analyzing recorded raw signals in the time-domain. Ongoing research tries to link these behaviors to different pasture characteristics and performs a refined signal processing analysis for a better monitoring of some possible behavioral changes. [less ▲]

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See detailThe vegetative state/unresponsive wakefulness syndrome: a systematic review of prevalence studies
Van Erp, WS; Lavrijsen, JC; Van De Laar, FA et al

in European Journal of Neurology (2014)

One of the worst outcomes of acquired brain injury is the vegetative state, recently renamed ‘unresponsive wakefulness syndrome’ (VS/UWS). A patient in VS/UWS shows reflexive behaviour such as spontaneous ... [more ▼]

One of the worst outcomes of acquired brain injury is the vegetative state, recently renamed ‘unresponsive wakefulness syndrome’ (VS/UWS). A patient in VS/UWS shows reflexive behaviour such as spontaneous eye opening and breathing, but no signs of awareness of the self or the environment. We performed a systematic review of VS/UWS prevalence studies and assessed their reliability. Medline, Embase, the Cochrane Library, CINAHL and PsycINFO were searched in April 2013 for cross-sectional point or period prevalence studies explicitly stating the prevalence of VS/UWS due to acute causes within the general population. We additionally checked bibliographies and consulted experts in the field to obtain ‘grey data’ like government reports. Relevant publications underwent quality assessment and data-extraction. We retrieved 1032 papers out of which 14 met the inclusion criteria. Prevalence figures varied from 0.2 to 6.1 VS/UWS patients per 100 000 members of the population. However, the publications’ methodological quality differed substantially, in particular with regards to inclusion criteria and diagnosis verification. The reliability of VS/UWS prevalence figures is poor. Methodological flaws in available prevalence studies, the fact that 5/14 of the studies predate the identification of the minimally conscious state (MCS) as a distinct entity in 2002, and insufficient verification of included cases may lead to both overestimation and underestimation of the actual number of patients in VS/UWS. [less ▲]

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See detailLiver proteomic response to hypertriglyceridemia in human-apolipoprotein C-III transgenic mice at cellular and mitochondrial compartment levels
Ehx, Grégory ULg; Gerin, Stéphanie ULg; Mathy, Grégory et al

in Lipids in Health and Disease (2014), 13

Background: Hypertriglyceridemia (HTG) is defined as a triglyceride (TG) plasma level exceeding 150 mg/dl and is tightly associated with atherosclerosis, metabolic syndrome, obesity, diabetes and acute ... [more ▼]

Background: Hypertriglyceridemia (HTG) is defined as a triglyceride (TG) plasma level exceeding 150 mg/dl and is tightly associated with atherosclerosis, metabolic syndrome, obesity, diabetes and acute pancreatitis. The present study was undertaken to investigate the impact of hypertriglyceridemia on the mitochondrial, sub-mitochondrial and cellular proteomes in the hepatocytes of a hypertriglyceridemic transgenic mouse model overexpressing the human apolipoproteinC-III. Methods: Quantitative comparative proteomics (2D-DIGE) was carried out in both “low-expressor” (LE) and “high-expressor” (HE) mice, respectively exhibiting moderate and severe HTG, to characterize the effect of the TG plasma level on the proteomic response. Results: The mitoproteome analysis revealed the occurrence of a large-scale adaptation in transgenic mice consisting of a general down-regulation of matricial proteins and up-regulation of inner membrane proteins. Remarkably, the magnitude of these proteomic changes appears to strongly depend on the TG plasma level. Altogether, our different analyses indicate that, in HE mice, the capacity of several metabolic pathways is altered to promote the availability of acetyl-CoA, glycerol-3-phosphate, ATP and NADPH for de novo TG biosynthesis. The up-regulation of several cytosolic ROS detoxifying enzymes also tend to confirm that the cytoplasm of HTG mice is subjected to oxidative stress as previously stated. The up-regulation of cytosolic ferritin indicates that iron over-accumulation could take place in the cytosol of HE mice hepatocytes and contribute to (i) enhance oxidative stress and (ii) promote cellular proliferation. Conclusions: The present analyses demonstrate that important TG dose-responsive metabolic adaptations are set up in human apolipoproteinC-III-overexpressing mice. Our results indicate that these adaptations could support the higher TG production rates which have been previously reported in this HTG model, and also suggest that cytosolic oxidative stress may result from FFA over-accumulation, iron overload and enhanced activity of some ROS-producing catabolic enzymes. [less ▲]

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See detailSeed dispersal by western lowland gorillas: what about gut passage effect?
Haurez, Barbara ULg; Petre, Charles-Albert ULg; Doucet, Jean-Louis ULg

Conference (2014, July 21)

Animal-mediated seed dispersal is an essential ecological process in the tropics. Among African primates, the critically endangered western lowland gorilla (WLG) seems to fulfill many characteristics of ... [more ▼]

Animal-mediated seed dispersal is an essential ecological process in the tropics. Among African primates, the critically endangered western lowland gorilla (WLG) seems to fulfill many characteristics of an effective seed disperser. WLG preferentially disperses species of various seed-size into open canopy habitats suitable for seedling growth. However, few studies have addressed the effects of the passage through the digestive tract on germination. Thus, the effectiveness of WLG in ecological processes governing forest dynamics and regeneration is still poorly known. Seed dispersal by WLG was studied in Central Gabon and Southeast Cameroon. Dispersed seed were identified from fresh fecal samples. Comparative germination trials based on 5 treatments (seeds from fresh fruits, seeds from fresh fruits surrounded by pulp, seeds from fresh fruits with fecal matrix, seeds from feces and seeds from feces with fecal matrix) were realized for 13 species. We recorded germination success for all species and germination delay for 6 species. Our results suggest a neutral gut passage effect for 54% and positive effect for 40% of tested species. Germination success enhancement was linked to another factor than pulp suppression. For two species, pulp suppression enhanced germination success, but not gut passage. The presence of pulp or fecal matrix surrounding the seed seemed to longer germination delay, while gut passage would not fasten germination. Globally, we conclude that WLG dispersed seeds are not damaged by gut passage. Consequently, WLG is thought to provide effective ecological services that could be essential to the maintenance and the recovery of forest ecosystems. [less ▲]

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