Last 7 days
Bookmark and Share    
Full Text
See detailMyoferlin, a new autophagy player in pancreatic cancer cells
Rademaker, Gilles ULg; Hennequière, Vincent ULg; Peixoto, Paul et al

Poster (2017, February 01)

Despite intensive research, Pancreatic Ductal Adenocarcinoma (PDAC) remains one of the deadliest forms of cancer. Early-stage of the disease is clinically silent and the diagnosis of the disease is mostly ... [more ▼]

Despite intensive research, Pancreatic Ductal Adenocarcinoma (PDAC) remains one of the deadliest forms of cancer. Early-stage of the disease is clinically silent and the diagnosis of the disease is mostly made at an advanced stage. This late diagnosis contributes to one of the lowest 5-year survival rates (<5%). Today, PDAC are treated by surgery and/or adjuvant therapy, increasing only slightly the median survival of the patients. There is therefore an urgent need to develop new effective therapies for PDAC patients. PDAC are characterized by a high autophagic activity involved in its chemoresistance. Recently, key regulatory proteins controlling the metabolic reprogramming of PDAC cells were identified. By governing both autophagic flux and lysosomal catabolism, these proteins support the efficient processing of cargo from autophagy, providing PDAC cells with access to critical sources of nutrients. Interestingly, the high autophagy level in PDAC correlates with a poor patient outcome. Myoferlin, a member of the ferlin family overexpressed at protein level in different cancer types including PDAC, is a transmembrane protein able to bound to phospholipids and described to play an important function in membrane fusion. This characteristic invited us to investigate whether myoferlin could participate to autophagy, a process involving membrane fusion. Panc-1 cell line was used as a model of PDAC basal autophagy. Myoferlin expression was silenced using interfering RNA technology. Autophagosome abundance was evaluated by LC3-II western-blot and flow cytometry. Results indicated a significant increase in autophagosome abundance 48 h after myoferlin-silencing. This increase could arise from an increase of autophagy initiation or from an inhibition of autolysosome degradation. Using autophagy inhibitors, autophagic flux was evaluated by LC3-II and p62 western-blot after myoferlin-silencing. Results suggested a blockade in the autophagic process, impairing termination and autophagosome degradation by lysosome activity. Knowing the affinity of myoferlin for phospholipids, we wonder if this protein could interact with the phosphatidylethanolamine-conjugated LC3-II protein. Proximity-ligation assay suggested a close interaction between myoferlin and LC3. These results evoke an unexplored and undescribed role for myoferlin in autophagy. Understanding the involvement of myoferlin in this rediscovered biological process could give new clues in the development of new therapeutic strategy. [less ▲]

Detailed reference viewed: 26 (6 ULg)
See detailCellular stress and β-lactamase BlaP induction in Bacillus licheniformis
Dauvin, Marjorie ULg; Joris, Bernard ULg

Poster (2017, February 01)

In presence of β-lactam antibiotic in its environment, B. licheniformis produces the β-lactamase BlaP, an enzyme hydrolyzing β-lactam antibiotic, conferring on the bacteria phenotypic resistance. To ... [more ▼]

In presence of β-lactam antibiotic in its environment, B. licheniformis produces the β-lactamase BlaP, an enzyme hydrolyzing β-lactam antibiotic, conferring on the bacteria phenotypic resistance. To induce BlaP, two conditions must be fulfilled. The first one is the acylation of the membrane receptor BlaR1 by the antibiotic. The second one is a cellular stress due to the presence of the antibiotic which acylate PBP1. The nature of this signal remains unknown. In this study we postulate that the extracytoplasmic function sigma factors (ECFs) σM and σX act together with the stringent response as a secondary and redundant layer of stress upon which the BlaP induction pathway relies. [less ▲]

Detailed reference viewed: 16 (4 ULg)
Full Text
Peer Reviewed
See detailStrong XUV irradiation of the Earth-sized exoplanets orbiting the ultracool dwarf TRAPPIST-1
Wheatley, Peter J.; Louden, Tom; Bourrier, Vincent et al

in Monthly Notices of the Royal Astronomical Society (2017), 465

We present an XMM-Newton X-ray observation of TRAPPIST-1, which is an ultracool dwarf star recently discovered to host three transiting and temperate Earth-sized planets. We find the star is a relatively ... [more ▼]

We present an XMM-Newton X-ray observation of TRAPPIST-1, which is an ultracool dwarf star recently discovered to host three transiting and temperate Earth-sized planets. We find the star is a relatively strong and variable coronal X-ray source with an X-ray luminosity similar to that of the quiet Sun, despite its much lower bolometric luminosity. We find L_x/L_bol=2-4x10^-4, with the total XUV emission in the range L_xuv/L_bol=6-9x10^-4. Using a simple energy-limited model we show that the relatively close-in Earth-sized planets, which span the classical habitable zone of the star, are subject to sufficient X-ray and EUV irradiation to significantly alter their primary and perhaps secondary atmospheres. Understanding whether this high-energy irradiation makes the planets more or less habitable is a complex question, but our measured fluxes will be an important input to the necessary models of atmospheric evolution. [less ▲]

Detailed reference viewed: 69 (1 ULg)
Full Text
See detailXMM-Newton : en route vers une autre décennie de découvertes
Rauw, Grégor ULg

Article for general public (2017)

Au mois de décembre 2016, cela faisait 17 ans que XMM-Newton fut lancé par une fusée Ariane V. Equipé de ses trois modules miroirs comportant chacun 58 miroirs imbriqués les uns dans les autres, XMM ... [more ▼]

Au mois de décembre 2016, cela faisait 17 ans que XMM-Newton fut lancé par une fusée Ariane V. Equipé de ses trois modules miroirs comportant chacun 58 miroirs imbriqués les uns dans les autres, XMM-Newton est à l’affût de l’émission X de sources cosmiques de tout genre. Malgré sa longévité, XMM-Newton reste à ce jour un des observatoires les plus sollicités au monde. Chaque année, les astrophysiciens proposent des programmes d’observation qui totalisent à peu près 6 fois le temps d’observation disponible! La compétition pour les observations avec XMM est donc très rude. Et a priori cela pourrait continuer encore comme cela pendant quelques années. De fait, les réserves de carburant et la performance des panneaux solaires indiquent que la mission pourrait encore fonctionner au moins 10 ans si les budgets le permettent1! [less ▲]

Detailed reference viewed: 23 (0 ULg)
Full Text
Peer Reviewed
See detailGlomerular filtration rate in healthy living potential kidney donors: a meta-analysis supporting the construction of the the Full Age Spectrum equation.
Pottel, H; Hoste, L; Yayo, E et al

in Nephron (2017), 135(2), 105-119

Detailed reference viewed: 31 (1 ULg)
See detailAttack-Prevention and Damage-Control Investments in Cybersecurity
Lam, Wing Man Wynne ULg

Scientific conference (2017, February)

Detailed reference viewed: 18 (1 ULg)
Full Text
Peer Reviewed
See detailLa réforme de l'aide juridique : suivez le guide !
Michiels, Olivier ULg; Falque, Géraldine ULg

in Les droits du justiciable face à la justice pénale (2017)

Detailed reference viewed: 34 (4 ULg)
Full Text
Peer Reviewed
See detailIntention, image et singularité selon le premier Sartre : réflexion sur les propriétés et les fonctions de l'acte d'imagination
Recchia, Fabio ULg

in Bulletin d'Analyse Phénoménologique (2017, February)

Partant d’une problématisation de la méthode phénoménologique mise en jeu par "L’Imaginaire", nous proposons de relire les développements de ce texte à partir de la perspective offerte par la théorie de ... [more ▼]

Partant d’une problématisation de la méthode phénoménologique mise en jeu par "L’Imaginaire", nous proposons de relire les développements de ce texte à partir de la perspective offerte par la théorie de l’intentionnalité du premier Sartre. Nous tenterons ainsi de dégager l’unité systématique du projet sartrien d’une « phénoménologie de l’image » en le rapportant aux propositions philosophiques posées de manière décisive par "l’article sur l’intentionnalité". Par ce biais, il s’agira également de fixer les propriétés de la conscience imageante, ainsi que les fonctions jouées par l’acte d’imagination dans l’économie générale de la conscience, en particulier dans l’événement de sa personnalisation. De sorte que nous parcourons les tensions reliant l’imagination de la conscience à son ipséité ; tandis que, du point de vue de l’histoire de la philosophie, nous examinerons certaines des torsions qu’appliqua Sartre sur l’analyse husserlienne de l’image afin d’approfondir celle-ci dans la direction d’une philosophie de la liberté personnelle du sujet. [less ▲]

Detailed reference viewed: 23 (4 ULg)
Full Text
Peer Reviewed
See detailClinical settings in knee osteoarthritis: Pathophysiology guides treatment
Herrero-Beaumont, Gabriel; Roman-Blas, Jorge A; Bruyère, Olivier ULg et al

in Maturitas (2017), 96

Osteoarthritis (OA) is the most common chronic joint disorder and its prevalence increases rapidly during midlife. Complex interactions of genetic alterations, sex hormone deficit, and aging with ... [more ▼]

Osteoarthritis (OA) is the most common chronic joint disorder and its prevalence increases rapidly during midlife. Complex interactions of genetic alterations, sex hormone deficit, and aging with mechanical factors and systemic inflammation-associated metabolic syndrome lead to joint damage. Thus, the expression of a clinical phenotype in the early stages of OA relies on the main underlying pathway and predominant joint tissue involved at a given time. Moreover, OA often coexists with other morbidities in the same patient, which in turn condition the OA process. In this scenario, an appropriate identification of clinical phenotypes, especially in the early stages of the disease, may optimize the design of individualized treatments in OA. An ESCEO-EUGMS (European Union Geriatric Medicine Society) working group has recently suggested possible patient profiles in OA. Hereby, we propose the existence of 4 clinical phenotypes – biomechanical, osteoporotic, metabolic and inflammatory – whose characterization would help to properly stratify patients with OA in clinical trials or studies. Further research in this field is warranted. [less ▲]

Detailed reference viewed: 32 (6 ULg)
Full Text
Peer Reviewed
See detailQuantitative determination of salbutamol sulfate impurities using achiral supercritical fluid chromatography
Dispas, Amandine ULg; Desfontaine, Vincent; Andri, Bertyl ULg et al

in Journal of Pharmaceutical & Biomedical Analysis (2017), 134

In the last years, supercritical fluid chromatography has largely been acknowledged as a singular and performing technique in the field of separation sciences. Recent studies highlighted the interest of ... [more ▼]

In the last years, supercritical fluid chromatography has largely been acknowledged as a singular and performing technique in the field of separation sciences. Recent studies highlighted the interest of SFC for the quality control of pharmaceuticals, especially in the case of the determination of the active pharmaceutical ingredient (API).Nevertheless, quality control requires also the determination of impurities. The objectives of the present work were to i) demonstrate the interest of SFC as a reference technique for the determination of impurities in salbutamol sulfate API and ii) to propose an alternative to a reference HPLC method from the European Pharmacopeia (EP) involving ionpairing reagent. Firstly, a screening was carried out to select the most adequate and selective stationary phase. Secondly, in the context of robust optimization strategy, the method was developed using design space methodology. The separation of salbutamol sulfate and related impurities was achieved in 7 minutes, which is seven times faster than the LC-UV method proposed by European Pharmacopeia (total run time of 50 minutes). Finally, full validation using accuracy profile approach was successfully achieved for the determination of impurities B, D, F and G in salbutamol sulfate raw material. The validated dosing range covered 50 to 150 % of the targeted concentration (corresponding to 0.3 % concentration level), LODs close to 0.5 μg/mL were estimated. The SFC method proposed in this study could be presented as a suitable fast alternative to EP LC method for the quantitative determination of salbutamol impurities. [less ▲]

Detailed reference viewed: 24 (6 ULg)
Full Text
Peer Reviewed
See detailLes acteurs: introduction
Dubois, Christophe ULg

in Hendrick, Aude; Musin, Aude; Rousseaux, Xavier (Eds.) et al Les mots de la justice (2017)

Detailed reference viewed: 13 (0 ULg)
Full Text
Peer Reviewed
See detailVictime
Dubois, Christophe ULg; Martin, Daniel

in Hendrick, Aude; Musin, Aude; Rousseaux, Xavier (Eds.) et al Les mots de la justice (2017)

Detailed reference viewed: 18 (3 ULg)