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See detailDifferential effects of context on psychomotor sensitization to ethanol and cocaine
Didone, Vincent ULg; Quoilin, Caroline; Dieupart, Julie et al

in Behavioural Pharmacology (2016), 27(2 & 3), 173-181

Repeated drug injections lead to sensitization of their stimulant effects in mice, a phenomenon sometimes referred to as drug psychomotor sensitization. Previous studies showed that sensitization to ... [more ▼]

Repeated drug injections lead to sensitization of their stimulant effects in mice, a phenomenon sometimes referred to as drug psychomotor sensitization. Previous studies showed that sensitization to cocaine is context dependent as its expression is reduced in an environment that was not paired with cocaine administration. In contrast, the effects of the test context on ethanol sensitization remain unclear. In the present study, female OF1 mice were repeatedly injected with 1.5 g/kg ethanol to test for both the effects of context novelty/familiarity and association on ethanol sensitization. A first group of mice was extensively pre-exposed to the test context before ethanol sensitization and ethanol injections were paired with the test context (familiar and paired group). A second group was not pre-exposed to the test context, but ethanol injections were paired with the test context (nonfamiliar and paired group). Finally, a third group of mice was not pre-exposed to the test context and ethanol was repeatedly injected in the home cage (unpaired group). Control groups were similarly exposed to the test context, but were injected with saline. In a second experiment, cocaine was used as a positive control. The same behavioral procedure was used, except that mice were injected with 10 mg/kg cocaine instead of ethanol. The results show a differential involvement of the test context in the sensitization to ethanol and cocaine. Cocaine sensitization is strongly context dependent and is not expressed in the unpaired group. In contrast, the expression of ethanol sensitization is independent of the context in which it was administered, but is strongly affected by the relative novelty/familiarity of the environment. Extensive pre-exposure to the test context prevented the expression of ethanol sensitization. One possible explanation is that expression of ethanol sensitization requires an arousing environment. [less ▲]

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See detailProtoporphyrin IX Functionalised AgSiO2 Core-shell Nanoparticle: Plasmonic Enhancement of Fluorescence and Singlet Oxygen Production
Lismont, Marjorie ULg; Dreesen, Laurent ULg; Heinrichs, Benoît ULg et al

in Photochemistry & Photobiology (2016), 92

Metal-enhanced processes arising from the coupling of a dye with metallic nanoparticles (NPs) have been widely reported. However, few studies have simultaneously investigated these mechanisms from the ... [more ▼]

Metal-enhanced processes arising from the coupling of a dye with metallic nanoparticles (NPs) have been widely reported. However, few studies have simultaneously investigated these mechanisms from the viewpoint of dye fluorescence and photoactivity. Herein, protoporphyrin IX (PpIX) is grafted onto the surface of silver core silica shell NPs in order to investigate the effect of silver (Ag) localized surface plasmon resonance (LSPR) on PpIX fluorescence and PpIX singlet oxygen (1O2) production. Using two Ag core sizes, we report a systematic study of these photophysical processes as a function of silica (SiO2) spacer thickness, LSPR band position and excitation wavelength. The excitation of Ag NP LSPR, which overlaps the PpIX absorption band, leads to the concomitant enhancement of PpIX fluorescence and 1O2 production independently of the Ag core size, but in a more pronounced way for larger Ag cores. These enhancements result from the increase in the PpIX excitation rate through the LSPR excitation and decrease when the distance between PpIX and Ag NPs increases. A maximum fluorescence enhancement of up to 14-fold, together with an increase in photogenerated 1O2 production of up to five times are obtained using 100 nm Ag cores coated with a 5 nm thick silica coating. [less ▲]

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See detailEvaluation of a New Method for the Treatment of Invasive, Diffuse, and Unexcisable Lymphangiomas of the Oral Cavity with Defocus CO2 Laser Beam: A 20-Year Follow-Up
Namour, Samir ULg; VAN HEUSDEN, Alain ULg; Namour, Amaury et al

in Photomedicine and Laser Surgery (2016), 34(2), 82-87

Abstract Objective: The aim of our study is to evaluate the efficiency of a new therapeutic method using CO2 laser to avoid mutilation or total excision for the treatment of invasive, large and ... [more ▼]

Abstract Objective: The aim of our study is to evaluate the efficiency of a new therapeutic method using CO2 laser to avoid mutilation or total excision for the treatment of invasive, large and unexcisable oral lymphangiomas. Background data: Cystic lymphatic malformations, or lymphangiomas, are mature lymphatic malformations, consisting of abnormal lymphatic vessels. Surgical excision is considered by most surgeons to be the treatment of choice for lymphangioma. Laser beam has been used to treat superficial or small lymphatic malformations by excision or by photocoagulation, and to excise or photocoagulate small lymphangiomas. Patients and methods: Seventeen patients with diffuse and unexcisable lymphangiomas were treated using a CO2 laser with specific settings: beam was 2W in noncontact and defocus mode, power density was 0.63W/cm2 for irradiation time in the range of 3– 5min, and the estimated energy density range was 114.65–191.02 J/cm2. The beam diameter at the tissular impact point was –2 cm. The laser beam was defocused to provoke a deep heat generation that would dry up the lymphatic tissues and induce deep tissue necrosis that heals by fibrous tissue process. Results: The results were satisfactory and stable, with localized recurrence in three cases. The recurrent areas were re-treated using the same technique successfully and with no further recurrence. Conclusions: The use of CO2 laser under the suggested irradiation conditions can be considered a useful technique for the treatment of invasive lymphangiomas. [less ▲]

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See detailBovine Herpesvirus 4 Modulates Its beta-1,6-N-Acetylglucosaminyltransferase Activity through Alternative Splicing.
Lété, Céline ULg; Markine-Goriaynoff, Nicolas; Machiels, Bénédicte ULg et al

in Journal of virology (2016), 90(4), 2039-51

Carbohydrates play major roles in host-virus interactions. It is therefore not surprising that, during coevolution with their hosts, viruses have developed sophisticated mechanisms to hijack for their ... [more ▼]

Carbohydrates play major roles in host-virus interactions. It is therefore not surprising that, during coevolution with their hosts, viruses have developed sophisticated mechanisms to hijack for their profit different pathways of glycan synthesis. Thus, the Bo17 gene of Bovine herpesvirus 4 (BoHV-4) encodes a homologue of the cellular core 2 protein beta-1,6-N-acetylglucosaminyltransferase-mucin type (C2GnT-M), which is a key player for the synthesis of complex O-glycans. Surprisingly, we show in this study that, as opposed to what is observed for the cellular enzyme, two different mRNAs are encoded by the Bo17 gene of all available BoHV-4 strains. While the first one corresponds to the entire coding sequence of the Bo17 gene, the second results from the splicing of a 138-bp intron encoding critical residues of the enzyme. Antibodies generated against the Bo17 C terminus showed that the two forms of Bo17 are expressed in BoHV-4 infected cells, but enzymatic assays revealed that the spliced form is not active. In order to reveal the function of these two forms, we then generated recombinant strains expressing only the long or the short form of Bo17. Although we did not highlight replication differences between these strains, glycomic analyses and lectin neutralization assays confirmed that the splicing of the Bo17 gene gives the potential to BoHV-4 to fine-tune the global level of core 2 branching activity in the infected cell. Altogether, these results suggest the existence of new mechanisms to regulate the activity of glycosyltransferases from the Golgi apparatus. IMPORTANCE: Viruses are masters of adaptation that hijack cellular pathways to allow their growth. Glycans play a central role in many biological processes, and several studies have highlighted mechanisms by which viruses can affect glycosylation. Glycan synthesis is a nontemplate process regulated by the availability of key glycosyltransferases. Interestingly, bovine herpesvirus 4 encodes one such enzyme which is a key enzyme for the synthesis of complex O-glycans. In this study, we show that, in contrast to cellular homologues, this virus has evolved to alternatively express two proteins from this gene. While the first one is enzymatically active, the second results from the alternative splicing of the region encoding the catalytic site of the enzyme. We postulate that this regulatory mechanism could allow the virus to modulate the synthesis of some particular glycans for function at the location and/or the moment of infection. [less ▲]

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See detailStructural Proteomics of Herpesviruses.
Leroy, Baptiste; Gillet, Laurent ULg; Vanderplasschen, Alain ULg et al

in Viruses (2016), 8(2),

Herpesviruses are highly prevalent viruses associated with numerous pathologies both in animal and human populations. Until now, most of the strategies used to prevent or to cure these infections have ... [more ▼]

Herpesviruses are highly prevalent viruses associated with numerous pathologies both in animal and human populations. Until now, most of the strategies used to prevent or to cure these infections have been unsuccessful because these viruses have developed numerous immune evasion mechanisms. Therefore, a better understanding of their complex lifecycle is needed. In particular, while the genome of numerous herpesviruses has been sequenced, the exact composition of virions remains unknown for most of them. Mass spectrometry has recently emerged as a central method and has permitted fundamental discoveries in virology. Here, we review mass spectrometry-based approaches that have recently allowed a better understanding of the composition of the herpesvirus virion. In particular, we describe strategies commonly used for proper sample preparation and fractionation to allow protein localization inside the particle but also to avoid contamination by nonstructural proteins. A collection of other important data regarding post-translational modifications or the relative abundance of structural proteins is also described. This review also discusses the poorly studied importance of host proteins in herpesvirus structural proteins and the necessity to develop a quantitative workflow to better understand the dynamics of the structural proteome. In the future, we hope that this collaborative effort will assist in the development of new strategies to fight these infections. [less ▲]

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See detailThe syntax-semantics Interface in Earlier Egyptian: A Case study in Verbs of Cognition
Winand, Jean ULg

in Coping with Obscurity: The Brown Workshop on Earlier Egyptian Grammar (2016)

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See detailCOMPARISON OF ULTRASOUND-GUIDED VS. STANDARD LANDMARK TECHNIQUES FOR TRAINING NOVICE OPERATORS IN PLACING NEEDLES INTO THE LUMBAR SUBARACHNOID SPACE OF CANINE CADAVERS
Etienne, Anne-Laure ULg; Delguste, Catherine ULg; Busoni, Valeria ULg

in Veterinary Radiology & Ultrasound (2016)

The standard technique for placing a needle into the canine lumbar subarachnoid space is primarily based on palpation of anatomic landmarks and use of probing movements of the needle, however, this ... [more ▼]

The standard technique for placing a needle into the canine lumbar subarachnoid space is primarily based on palpation of anatomic landmarks and use of probing movements of the needle, however, this technique can be challenging for novice operators. The aim of the current observational, prospective, ex vivo, feasibility study was to compare ultrasound-guided vs. standard anatomic landmark approaches for novices performing needle placement into the lumbar subarachnoid space using dog cadavers. Eight experienced operators validated the canine cadaver model as usable for training landmark and ultrasound-guided needle placement into the lumbar subarachnoid space based on realistic anatomy and tissue consistency. With informed consent, 67 final year veterinary students were prospectively enrolled in the study. Students had no prior experience in needle placement into the lumbar subarachnoid space or use of ultrasound. Each student received a short theoretical training about each technique before the trial and then attempted blind landmark-guided and ultrasound-guided techniques on randomized canine cadavers. After having performed both procedures, the operators completed a self-evaluation questionnaire about their performance and self-confidence. Total success rates for students were 48% and 77% for the landmark- and ultrasound-guided techniques, respectively. Ultrasound guidance significantly increased total success rate when compared to the landmark-guided technique and significantly reduced the number of attempts. With ultrasound guidance self-confidence was improved, without bringing any significant change in duration of the needle placement procedure. Findings indicated that use of ultrasound guidance and cadavers are feasible methods for training novice operators in needle placement into the canine lumbar subarachnoid space. [less ▲]

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See detailContext-dependent roles for lymphotoxin-beta receptor signaling in cancer development.
Fernandes, Monica T.; Dejardin, Emmanuel ULg; Santos, Nuno R. Dos

in Biochimica et biophysica acta (2016)

The LTalpha1beta2 and LIGHT TNF superfamily cytokines exert pleiotropic physiological functions through the activation of their cognate lymphotoxin-beta receptor (LTbetaR). Interestingly, since the ... [more ▼]

The LTalpha1beta2 and LIGHT TNF superfamily cytokines exert pleiotropic physiological functions through the activation of their cognate lymphotoxin-beta receptor (LTbetaR). Interestingly, since the discovery of these proteins, accumulating evidence has pinpointed a role for LTbetaR signaling in carcinogenesis. Early studies have shown a potential anti-tumoral role in a subset of solid cancers either by triggering apoptosis in malignant cells or by eliciting an anti-tumor immune response. However, more recent studies provided robust evidence that LTbetaR signaling is also involved in diverse cell-intrinsic and microenvironment-dependent pro-oncogenic mechanisms, affecting several solid and hematological malignancies. Consequently, the usefulness of LTbetaR signaling axis blockade has been investigated as a potential therapeutic approach for cancer. Considering the seemingly opposite roles of LTbetaR signaling in diverse cancer types and their key implications for therapy, we here extensively review the different mechanisms by which LTbetaR activation affects carcinogenesis, focusing on the diverse contexts and different models assessed. [less ▲]

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See detailInfluence of crossed fields in structures combining large grain, bulk (RE)BCO superconductors and soft ferromagnetic discs
Philippe, Matthieu ULg; Fagnard, Jean-François ULg; Wera, Laurent ULg et al

in Journal of Physics: Conference Series (2016), 695

Bulk (RE)BCO superconductors are able to trap record magnetic fields and can be used as powerful permanent magnets in various engineering applications such as rotating machines and magnetic bearings. When ... [more ▼]

Bulk (RE)BCO superconductors are able to trap record magnetic fields and can be used as powerful permanent magnets in various engineering applications such as rotating machines and magnetic bearings. When such superconducting (SC) “trapped field magnets” are combined to a ferromagnetic (FM) disc, the total magnetic moment is increased with respect to that of the superconductor alone. In the present work, we study experimentally the magnetic behaviour of such hybrid FM/SC structures when they are subjected to cycles of applied field that are orthogonal to their permanent magnetization, i.e. a “crossed-field” configuration. Experimental results show that the usual “crossed-field demagnetization” caused by the cycles of transverse field is strongly reduced in the presence of the ferromagnet. [less ▲]

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See detailDefining multiplication in some additive expansions of polynomial rings
Point, Françoise; Rigo, Michel ULg; Waxweiler, Laurent

in Communications in Algebra (2016), 44

Adapting a result of R. Villemaire on expansions of Presburger arithmetic, we show how to define multiplication in some expansions of the additive reduct of certain Euclidean rings. In particular, this ... [more ▼]

Adapting a result of R. Villemaire on expansions of Presburger arithmetic, we show how to define multiplication in some expansions of the additive reduct of certain Euclidean rings. In particular, this applies to polynomial rings over a finite field. [less ▲]

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See detailScreening for GPR101 defects in pediatric pituitary corticotropinomas.
Trivellin, Giampaolo; Correa, Ricardo R.; Batsis, Maria et al

in Endocrine-related cancer (2016)

Cushing disease (CD) in children is caused by adrenocorticotropic hormone (ACTH)-secreting pituitary adenomas. Germline or somatic mutations in genes such as MEN1, CDKIs, AIP, and USP8 have been ... [more ▼]

Cushing disease (CD) in children is caused by adrenocorticotropic hormone (ACTH)-secreting pituitary adenomas. Germline or somatic mutations in genes such as MEN1, CDKIs, AIP, and USP8 have been identified in pediatric CD, but the genetic defects in a significant percentage of cases are still unknown. We investigated the orphan G protein-coupled receptor GPR101, a gene known to be involved in somatotropinomas, for its possible involvement in corticotropinomas. We performed GPR101 sequencing, expression analyses by RT-qPCR and immunostaining, and functional studies (cell proliferation, pituitary hormones secretion, and cAMP measurement) in a series of patients with sporadic CD secondary to ACTH-secreting adenomas in whom we had peripheral and tumor DNA (N=36). No increased GPR101 expression was observed in tumors compared to normal pituitary (NP) tissues, nor did we find a correlation between GPR101 and ACTH expression levels. Sequence analysis revealed a very rare germline heterozygous GPR101 variant (p.G31S) in one patient with CD. Overexpression of the p.G31S variant did not lead to increased growth and proliferation, although modest effects on cAMP signaling were seen. GPR101 is not overexpressed in ACTH-secreting tumors compared to NPs. A rare germline GPR101 variant was found in one patient with CD but in vitro studies did not support a consistent pathogenic effect. GPR101 is unlikely to be involved in the pathogenesis of CD. [less ▲]

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See detailLa science (humaine) des chiens
Servais, Véronique ULg

Book published by Le bord de l'eau (2016)

L'ouvrage est divisé en deux parties. La première confronte différentes perspectives théoriques permettant de faire entrer le chien en sciences sociales. La seconde partie présente des études empiriques ... [more ▼]

L'ouvrage est divisé en deux parties. La première confronte différentes perspectives théoriques permettant de faire entrer le chien en sciences sociales. La seconde partie présente des études empiriques documentant la présence des chiens auprès des humains. [less ▲]

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See detailCritical analysis of the paramagnetic to ferromagnetic phase transition in Pr0.55K0.05Sr0.4MnO3
Thaljaoui, R.; Pękała, M.; Fagnard, Jean-François ULg et al

in Physica B: Condensed Matter (2016), 482

The critical properties of monovalent doped manganite Pr0.55K0.05Sr0.4MnO3 around the paramagnetic to ferromagnetic phase transition were investigated through various methods: the modified Arrott plots ... [more ▼]

The critical properties of monovalent doped manganite Pr0.55K0.05Sr0.4MnO3 around the paramagnetic to ferromagnetic phase transition were investigated through various methods: the modified Arrott plots (MAP), the Kouvel-Fisher method and the critical isotherm analysis. Data obtained near Tc were examined in the framework of the mean field theory, the 3D–Heisenberg model, the 3D–Ising model, and tricritical mean field. The deduced critical exponents values obtained using MAP method were found to be β = 0.44(4) with TC ~ 303 K and γ = 1.04(1) with TC ~ 302 K. Kouvel-Fisher method supplies the critical values to be β = 0.41(2) with TC ~ 302 K and γ = 1.09(1) with TC ~ 302 K. The obtained critical parameters show a tendency towards the mean-field behavior, suggesting the existence of long-range ferromagnetic order in the compound studied. The exponent δ deduced separately from isotherm analysis at T= 303 K was found to obey to the Widom scaling relation δ = 1+ γ/ β. The reliability of obtained exponents was confirmed by using the universal scaling hypothesis. The itinerant character of ferromagnetism in the present system was also tested by using Rhodes-Wohlfarth’s criterion. [less ▲]

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See detailNa2FePO4F/multi-walled carbon nanotubes for lithium-ion batteries: Operando Mössbauer study of spray-dried composites
Brisbois, Magali; Caes, Sébastien ULg; Sougrati, M.T. et al

in Solar Energy Materials & Solar Cells (2016), 148

In order to favor electronic conductivity in sodium iron fluorophosphate electrodes for lithium- or sodium-ion batteries, composites of Na2FePO4F with multi-walled carbon nanotubes (CNTs) were prepared by ... [more ▼]

In order to favor electronic conductivity in sodium iron fluorophosphate electrodes for lithium- or sodium-ion batteries, composites of Na2FePO4F with multi-walled carbon nanotubes (CNTs) were prepared by pilot-scale spray drying. Addition of multi-walled CNTs in the solution results in an excellent dispersion of the CNTs within the volume of Na2FePO4F and not only at the surface of the particles. Following a heat treatment at 600°C in argon in order to reach crystallization, X-ray diffraction and ex situ Mössbauer spectroscopy revealed the presence of significant amounts of Fe(III) and maghemite (γ-Fe2O3) in the powder. However, Na2FePO4F/CNTs composites exhibit good electrochemical performance when cycling against lithium, with a discharge capacity of 104mAhg-1 at C/10 rate and 90mAhg-1 at 1C rate. Therefore, operando 57Fe transmission Mössbauer spectroscopy analyses were carried out in order to investigate the evolution of the iron oxidation state during cycling. During the first discharge, all the Fe(III) is reduced to Fe(II), explaining the good electrochemical performance. [less ▲]

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See detailPas d'obstacle constitutionnel pour les sanctions administratives communales
Michiels, Olivier ULg

in Revue de la Faculté de Droit de l'Université de Liège (2016), (2016/1), 79-99

Le législateur, lors de l’instauration du système des sanctions administratives communales, a entendu combattre les incivilités. Cette notiond’«incivilité» vise des comportements dans l’espace public qui ... [more ▼]

Le législateur, lors de l’instauration du système des sanctions administratives communales, a entendu combattre les incivilités. Cette notiond’«incivilité» vise des comportements dans l’espace public qui s’écartent de ce qui est convenable dans la vie en société. En veillant à définir les «incivilités» comme les formes les plus légères de trouble de l’ordre public, de la sécurité, de la santé et de la propreté, qui sont dégradants ou incommodants et qui dépassent les contraintes normales de la vie sociale, le législateur a satisfait aux exigences du principe de légalité.Le droit à une bonne administration de la justice tel qu’il est garanti par l’article 6 de la Convention européenne des droits de l’homme, n’exclut pas qu’une sanction administrative soit infligée par un fonctionnaire, à condition qu’un juge indépendant et impartial puisse exercer un contrôle de pleine juridic-tion sur cette décision administrative. [less ▲]

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See detailShizuka Murayama (1918-2013)
De Marco, Rosa ULg

in Allgemeines Künstlerlexikon (AKL) (2016)

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See detailLa pensée future épisodique : entre simulation et contexte autobiographique
D'Argembeau, Arnaud ULg

in Revue de Neuropsychologie (2016), 8

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See detailIn vitro and in vivo evaluation of drug-eluting microspheres designed for transarterial chemoembolization therapy
Wang, Y.; Molin, D.; Sevrin, Chantal ULg et al

in International Journal of Pharmaceutics (2016), 503

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See detailThe role of personal goals in autonoetic experience when imagining future events
Lehner, Edith; D'Argembeau, Arnaud ULg

in Consciousness & Cognition (2016), 42

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See detailSynthesis of hydrophilic CuInS2/ZnS quantum dots with different polymeric shells and study of their cytotoxicity and hemocompatibility
Speranskaya, Elena Sergeevna; Sevrin, Chantal ULg; De Saeger, Sarah et al

in ACS Applied Materials and Interfaces (2016)

Detailed reference viewed: 16 (1 ULg)