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See detailObjective drowsiness monitoring to assess fitness for duty
François, Clémentine ULg; Hoyoux, Thomas ULg; Langohr, Thomas ULg et al

Poster (2016, September 16)

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See detailMetabolites from media supplemented with 3’-sialyllactose and fermented by bifidobacteria have an antivirulent effect against intestinal pathogens
Bondue, Pauline ULg

Poster (2016, September 16)

Introduction Complex oligosaccharides from human milk (HMO) promote growth of bifidobacteria such as Bifidobacterium bifidum [1]. Whey, a by-product of dairy-industry, contents complex oligosaccharides ... [more ▼]

Introduction Complex oligosaccharides from human milk (HMO) promote growth of bifidobacteria such as Bifidobacterium bifidum [1]. Whey, a by-product of dairy-industry, contents complex oligosaccharides (BMO) similar to HMO, which are mainly represented in colostrum by 3’-sialyllactose (3’SL) [2]. Bifidobacterium crudilactis, a species of bovine origin, encodes for β galactosidases and α-glucosidases and could therefore be able to metabolise those BMO [3; 4; 5]. In addition, fermentation products from bifidobacteria can produce antivirulent activity against intestinal pathogenic bacteria [6; 7]. This study focused on capacity of bifidobacteria to metabolise BMO, more particularly 3’SL, and on potential antivirulent effect of cell-free spent media (CFSM) against virulence gene expression of pathogenic bacteria. Material and methods B. bifidum BBA1 and B. crudilactis FR/62/B/3 isolated respectively from breastfed children feces and from cow raw milk cheese were grown on media supplemented with BMO or 3’SL, as sole source of carbon. The CFSM were harvested after centrifugation of cells culture, freeze-dried and concentrated 10 fold. Next, their effects were tested against virulence gene expression using ler and hilA promoter activity of luminescent constructs of Escherichia coli 0157:H7 ATCC 43888 and Salmonella Typhimurium SA 941256, respectively. The effect was confirmed on wild type strains of E. coli O157:H7 ATCC 43890 and S. Typhimurium ATCC 14028 using RT-qPCR. Results Both strains were able to grow in presence of whey or 3’SL, but B. crudilactis showed the best growth compared to B. bifidum. The highest cell concentrations were observed with media containing whey (8.9 ± 0.6 log cfu/ml and 8.1 ± 0.3 log cfu/ml, respectively). CFSM from fermented media supplemented with 3’SL resulted in under-expression of hilA and ler genes for the luminescent constructs and in under-expression of ler (ratios of -15.4 and -8.1) and qseA (ratios of -2.1 and -3.1) genes for the wild type strain of E. coli O157:H7. No effect was observed for the wild type strain of S. Typhimurium. Discussion B. crudilactis presented the best growth potential probably because its genome encodes the enzymatic machinery to use BMO (β galactosidases and α-glucosidases) [3; 4; 5]. The positive effect of media supplemented with milk products on growth of probiotics has been demonstrated previously [8]. CFSM obtained from media supplemented with 3’SL down-regulate several virulence genes of E. coli O157:H7 and potentially S. Typhimurium. This effect has been observed previously with CFSM obtained from fermentation of lactic acid bacteria or bifidobacteria, by production of antivirulent metabolites [2; 3]. BMO combined with some bifidobacteria strains of bovine or human origin could therefore be an interesting synbiotic to maintain or restore the intestinal health of young children. These effects observed in vitro will be further investigated regarding the exact nature of the active molecules. References 1. Garrido D. et al. (2013). Microbiology 159: 649-664. 2. Urashima T. et al. (2013). Biosci Biotechnol Biochem 77: 455-466. 3. Sela D. A. (2011). Int J Food Microbiol 149: 58-64. 4. Milani C. et al. (2014). Appl Environ Microbiol 80: 6290-6302. 5. Bondue P. & Delcenserie V. (2015). Korean J Food Sci Anim Resour 35: 1-9. 6. Medellin-Pena M. J. et al. (2007). Appl Environ Microbiol 73: 4259-4267. 7. Bayoumi M. A. & Griffiths M. W. (2012). Int J Food Microbiol 156: 255-263. 8. Champagne C. P. et al. (2014). Can J Microbiol 60: 287-295. [less ▲]

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See detailWhat’s the difference? Results of a functional study of Aterian and Mousterian tools from the site of Ifri n’Ammar (Morocco)
Tomasso, Sonja ULg; Rots, Veerle ULg

Conference (2016, September 16)

Until today, the definition of the North African Mousterian has been based on a systematic comparison with the European Mousterian. Particularly the “Aterian” and its tanged tools have been widely ... [more ▼]

Until today, the definition of the North African Mousterian has been based on a systematic comparison with the European Mousterian. Particularly the “Aterian” and its tanged tools have been widely discussed. Researchers considered the tanged Aterian tools as early indications of the existence of hafting techniques [1]. It is currently not entirely understood how the Aterian relates to the Mousterian in North Africa, whether tanged tools can indeed be linked with hafting, and whether non-tanged tools were also hafted, which could indicate that a variety in hafting techniques existed. The site of Ifri n’Ammar presents an ideal chance to compare Aterian and Mousterian technocomplexes. The rock shelter is located in the eastern Moroccan Rif and has a rich and well preserved stratigraphy where Middle Paleolithic tools are abundantly represented [2]. At Ifri n’Ammar, the Aterian and Mousterian assemblages are inter-stratified, which means that the relationship of these industries cannot simply be explained in terms of chronological succession [2,3]. The density of retouched artefacts differs between the Aterian and the Mousterian levels and tanged tools are present in the denser Aterian levels only. These levels also show a higher overall tool frequency. We present the results of a functional study focusing on the artefacts from the upper levels (“Occupation supérieure”) of Ifri n’Ammar, dated between 83 ± 6 ka and 130 ± 8 ka [3]. The functional study was combined with a specific experimental program designed to address questions raised during the analysis of the archaeological material, with a specific focus on hafting. Diagnostic microscopic wear patterns confirm that the tanged tools were used while hafted. Tanged tools did not prove to be related to hunting activities only, but various tool uses could be identified. They all fit, however, within the context of hunting and animal processing activities. The reuse of hafted armatures for other activities is not evident in the present sample. [less ▲]

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See detailLe centre monumental de Baelo Claudia. Rapport 2015-2016
Brassous, Laurent; Deru, Xavier ULg; Rodríguez Gutiérrez, Oliva et al

Report (2016)

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See detailWhat are the optimal walking tests to assess disability progression?
Pierard, Sébastien ULg; Van Droogenbroeck, Marc ULg

in Multiple Sclerosis Journal (2016, September 16), 22(S3), 452-453

Background. Therapy success is assumed when there is no evidence of disease activity. Clues to show it include an MRI, the relapses history, questionnaires, and clinical measures to assess the disability ... [more ▼]

Background. Therapy success is assumed when there is no evidence of disease activity. Clues to show it include an MRI, the relapses history, questionnaires, and clinical measures to assess the disability progression. Especially gait analysis plays a major role as gait impairment is considered by patients as the most disabling symptom. Too often only the walking speed is measured. New technologies (eg GAIMS, see ECTRIMS 2012-15) measure many spatiotemporal gait parameters, even during long tests (\eg 6min, 500m), without equipping patients with markers or sensors. Moreover, various tests can be done, depending on the length and type of walk (comfortable pace --C--, as fast as possible --F--, tandem gait --T--). Objective. Determine if there is an advantage to perform various walking tests, and which test or combination of tests brings the higher amount of information about the patient state in a reasonable amount of acquisition time. Methods. The system GAIMS provided 434 recordings of the gait parameters of healthy people and 60 recordings of MS patients with EDSS<= 4. They performed 12 tests (25ft C+F+T each twice, 20m C+F+T, 100m C+F, 500m F). To assess the ability of these clinical outcome measures to detect disability progression, we evaluate the possibility of differentiating the persons below a given EDSS threshold (0.25) from those above it based only on the measured gait parameters. For individual tests, we use the classifier of Azrour (ESANN 2014). All subsets of the tests are also considered, by combining the individual classifiers and determining automatically the optimal relative importance of the tests with the linear support vector machine (SVM) technique. The ability to detect the disability progression is quantified by the performance (area under the ROC curve --AUC-- and the maximum achievable balanced accuracy --MBA--) of the corresponding classifiers. Results. The best test alone is the 500m F (note that the walking speed measured during it is the gait parameter best correlated with the EDSS). Combining several tests leads to a better performance. A performance (MBA=95.7%, AUC=0.983) close to the best achievable one can be obtained with 6 tests only (25ft C twice, 25tf F twice, 20m C, 20m T). Conclusions. The clinical gait analysis can help to detect disability progression. While considering different types of walking tests improves the ability of taking decisions, we showed that performing 6 tests for a total of 70.48m suffices. [less ▲]

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See detailLes assemblées médiévales et leur héritage dans le droit constitutionnel contemporain
Behrendt, Christian ULg; Vandenbosch, Sofia ULg

Scientific conference (2016, September 16)

Conférence donnée dans le cadre du colloque international organisé à l'occasion du 700e anniversaire de la Paix de Fexhe (1316-2016), Liège, Palais provincial, 16 septembre 2016.

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See detailOn-farm Evolution of Genetic Diversity of Four Old Varieties of Phaseolus vulgaris L.
Caproni, Leonardo; Raggi, Lorenzo; Klaedtke, Stephanie ULg et al

Poster (2016, September 16)

On-farm evolution of crop genetic diversity has a key role in adaptation of agricultural systems to changes. The aim of this work was to understand how four different old common bean varieties, which ... [more ▼]

On-farm evolution of crop genetic diversity has a key role in adaptation of agricultural systems to changes. The aim of this work was to understand how four different old common bean varieties, which being made of different genotypes can be referred to as populations, evolved on-farm during three years of multiplication (from 2012 to 2014) in two different experimental sites (Brittany and Luxembourg) under organic farming conditions. The four populations (Flageolet Chevrier Vert, Rognon de Coq, Roi des Belges, Saint Esprit à Œil Rouge) were chosen among those grown by two small-scale organic seed farmers while the commercial variety (Calima) was included as control. In 2015 the initial and the evolved populations were grown in a common field and characterized with morpho-phenological traits. In the same year, young leaf tissues were collected from 94 samples per population (total of 470 samples) for DNA extraction and molecular characterization. Genotyping was carried out using 22 SSR loci and fluorescent PCR amplicons analysed on an ABI3130xl sequencer. Molecular data were then used for multivariate analysis, analysis of molecular variance and pair-wise Fst statistics calculation. Genotyping resulted in the production of about 20K data points. A total of 83 different alleles were identified in 16 polymorphic loci ranging from two (BMb293, BM156, BMd-44) to 18 (BMd-43). The first two axis of the Principal Coordinates analysis (PCoA) explained 76,13% of total diversity and clearly distinguished the five groups of populations. Analysis of Molecular Variance (AMOVA) showed that 76, 20 and 4% of total diversity is among populations, individuals and within individuals, respectively. Only for multiplications carried out in Luxemburg significant differentiation between the original populations and final populations were found in two old varieties (Flageolet Chevrier Vert and Rognon de Coq, Fst = 0.117 and 0.143, respectively, P≤0.001). The results showed that in Luxemburg farmer practices and pedo-climatic conditions significantly shaped the diversity of the two populations. This is confirmed by phenotypic observations, as these multiplications also significantly differed (p<0.05) for flowering date for all varieties except 'Roi des Belges'. [less ▲]

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See detailCoefficients binomiaux de mots
Rigo, Michel ULg

Scientific conference (2016, September 15)

Le coefficient binomial (u,v) de deux mots u et v est défini comme le nombre de fois que v apparaît comme sous-suite du mot u. Par exemple, (abbab,ab)=4. Il étend de manière naturelle le coefficient ... [more ▼]

Le coefficient binomial (u,v) de deux mots u et v est défini comme le nombre de fois que v apparaît comme sous-suite du mot u. Par exemple, (abbab,ab)=4. Il étend de manière naturelle le coefficient binomial de deux entiers. Ce concept a été largement étudié depuis plus d'une trentaine d'années (cf. par exemple, Simon et Sakarovitch). Dans cet exposé, je passerai tout d'abord en revue quelques résultats combinatoires classiques pour ensuite m'attarder sur l'équivalence k-binomiale. A l'instar de l'équivalence k-abélienne étudiée par Karhumäki et al., deux mots x et y sont k-binomialement équivalents si leurs coefficients binomiaux (x,v) et (y,v) coïncident pour les mots v de longueur au plus k. En fin d'exposé, j'évoquerai l'extension récente des triangles de Pascal et de Sierpinski à ces coefficients. [less ▲]

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See detailAcoustic indices provide information on the status of coral reefs: an example from Moorea Island in the South Pacific
Bertucci, Frédéric; Parmentier, Eric ULg; Lecellier, Gaël et al

in Scientific Reports (2016), 6(33326), 1-9

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See detailWhich role for State intervention in Collective bargaining? Measures to moderate wages in times of crisis : the belgian example
Kéfer, Fabienne ULg

Scientific conference (2016, September 15)

The belgian collective bargaining model has resisted since the crisis of 2008 and adapted. The mesures of moderating wages are negociated by de representative organisations. Since 2010, however ... [more ▼]

The belgian collective bargaining model has resisted since the crisis of 2008 and adapted. The mesures of moderating wages are negociated by de representative organisations. Since 2010, however, interprofessionnal bergaining is blocked. Interventions of public authority in the evolution of wages are subsidiary but reccurrent since. [less ▲]

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See detailDevelopment of a semiochemical-based control method against the walnut husk fly, Rhagoletis completa Cresson
Sarles, Landry ULg; Lognay, Georges ULg; Verhaeghe, Agnès et al

Poster (2016, September 15)

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See detailLa Paix de Fexhe : un texte, des acteurs
Masson, Christophe ULg

Conference (2016, September 15)

La Paix de Fexhe fut le résultat d’un compromis entre les cinq différents « partis » politiques liégeois de l’époque : le prince-évêque, le chapitre cathédral de Saint-Lambert, le « patriciat » urbain, la ... [more ▼]

La Paix de Fexhe fut le résultat d’un compromis entre les cinq différents « partis » politiques liégeois de l’époque : le prince-évêque, le chapitre cathédral de Saint-Lambert, le « patriciat » urbain, la noblesse et les artisans. Nous étudierons donc dans le cadre de cette contribution l’identité de ces partis, ainsi que celles des délégués qu’ils envoyèrent à Fexhe, leur « agenda politique » et leurs moyens d’action. Nous reviendrons ensuite sur la part que chacun prit à la rédaction du texte du 18 juin 1316 et de ses deux compléments ratifiés le même jour et le lendemain, ce qui nous permettra de mesurer la place que chacun put faire tenir à ses revendications, nuançant, pour le moins, la lecture « démocratique » ou « ecclésio-paternaliste » en cours depuis le xixe siècle. De façon complémentaire, nous mettrons en évidence les champs d’application et les limites de ces textes, en les articulant avec ceux qui les précédèrent afin de mieux en percevoir les implications sur la longue durée de l’histoire liégeoise. [less ▲]

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See detailIntroduction
Masson, Christophe ULg

Conference (2016, September 15)

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See detailPerformance de l'indicateur Azote Potentiellement Lessivable pour l'évaluation de la gestion du nitrate en agriculture et du risque pour la qualité de l’eau
Vandenberghe, Christophe ULg

Doctoral thesis (2016)

Since the end of last century, the nitrate concentration in groundwater has steadily increased in Wallonia as in other parts of Europe, particularly in regions where agriculture is intensively practised ... [more ▼]

Since the end of last century, the nitrate concentration in groundwater has steadily increased in Wallonia as in other parts of Europe, particularly in regions where agriculture is intensively practised. In response to this, Europe developed a ' Nitrates ' directive in 1991 that each member state had to transcribe in its legislation. In the Walloon region, the transcription took the form of a Sustainable Nitrogen Management Program (PGDA). This program provides among others control of farms through the analysis of nitrate nitrogen soil residue, in autumn, the beginning of the leaching period of nitrate. This measure is commonly referred to as APL for ‘Potentially Leachable Nitrogen’. The results are assessed by comparison to references established annually based on observations in plots where PGDA is applied and where fertilization recommendations are based on nitrogen balance. In case of non -compliance, the farmer has to follow an observation program, or even to pay a fine. The aim of the thesis is to evaluate the performance of the APL as agronomic (nitrogen management) and environmental indicator (risk to groundwater quality). On the agronomic side, through experimentations or treatment of field APL controls, it has been demonstrated that the indicator was well correlated with fertilization practices and intercrop management. The performance of the indicator has been validated. Some uncertainty factors (intra-plot variability, temporal variability, bulk density, stone content) were quantified. Finally, the influence of soil physicochemical properties on the APL was studied and discussed. The performance of the APL control was also discussed and improvements to regulation have been proposed. On the environmental side, through observations made during a decade in plots equipped with a lysimiter or in a small watershed, the dependence of the nitrate concentration in water (at the base of the root zone or in aquifer) to the APL has been demonstrated. It therefore appears that the APL can be used to firstly assess the reality of the sustainable nitrogen management in controlled farms and also to validate or, if necessary, complete the PGDA to make it more efficient for water quality preservation / restoration. [less ▲]

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See detailTHERMO-ECONOMIC OPTIMIZATION OF ORGANIC RANKINE CYCLE SYSTEMS FOR WASTE HEAT RECOVERY FROM EXHAUST AND RECIRCULATED GASES OF HEAVY DUTY TRUCKS
Guillaume, Ludovic ULg; Legros, Arnaud; Lemort, Vincent ULg

Conference (2016, September 14)

Waste heat recovery (WHR) ORC is a very promising technology for reducing fuel consumption and consequently the CO2 emissions of future heavy-duty trucks (HDT). Nonetheless, the adoption of this ... [more ▼]

Waste heat recovery (WHR) ORC is a very promising technology for reducing fuel consumption and consequently the CO2 emissions of future heavy-duty trucks (HDT). Nonetheless, the adoption of this technology in the automotive domain requires specific R&D activities going from the system definition to the on-board integration. This study focuses on the preliminary design phase of ORC systems recovering the heat wasted from two of the sources available on a HDT: the exhaust and recirculated gases. From these heat sources and their combinations, 6 possible architectures are identified. On the other hand, 4 volumetric expansion machine technologies are considered (Scroll, Screw, Piston and Vane Expanders). At the end, 24 topologies are modelled considering only the main components (Pump, Heat exchangers, Expansion machines). A three-step optimization method is proposed to identify the most promising system. First, the most suitable design conditions are identified using a simple model of expansion machine. In a second step, the design phase, using more detailed models for the expansion machines, a thermodynamic and economic optimizations are performed. Finally, in a third step, the output power of the latter system models is maximized in off-design conditions. [less ▲]

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