SAHBEN, an accessible surface-based elastic network to insert a protein in a complex lipid membrane

Poster (2013, February 26)

Study of membrane proteins have become one of the most challenging fields in biology. Solving their structure is one important step toward the understanding of their physiological activity but despite the ... [more ▼]

Study of membrane proteins have become one of the most challenging fields in biology. Solving their structure is one important step toward the understanding of their physiological activity but despite the recent advances in membrane protein crystallization, it represents less than 1 % of the entries in the Protein Data Bank. Therefore, calculation methods to study membrane proteins are helpful to complement experimental studies and fill the gap between the information obtained from the sequence and/or structure, the experimental results and the biological activity. Molecular Dynamics (MD) is a method of choice for membrane simulations and the rising of coarse-grained forcefields has opened the way to longer simulations with reduced calculations times. However, these approaches have two main drawbacks, the preparation of the membrane system and the preservation of the 3D protein structure, which is not trivial in CG approach. To circumvent these problems, we propose to use a modified version of the Gromacs tool genbox to easily insert lipids and a network based on hydrogen bonds and accessible surface to maintain the protein 3D structure. This protocol is available through a website (gcgs.gembloux.ulg.ac.be). [less ▲]

Structural and functional characterization of the Arabidopsis halleri HMA4 N-terminal domain

Poster (2013, February 26)

Characterization of monoclonal antibodies against Hemolysin Alpha of Staphylococcus aureus

Poster (2013, February 26)

In this study, vaccine-based targeting of Hemolysin Alpha (HLA) provides protection against lethal staphylococcal infection in a murine model system, suggesting that a monoclonal antibody-based therapy ... [more ▼]

In this study, vaccine-based targeting of Hemolysin Alpha (HLA) provides protection against lethal staphylococcal infection in a murine model system, suggesting that a monoclonal antibody-based therapy may likewise prove to be efficacious for prevention and treatment of this infection. We report the generation of four distinct anti-α-hemolysin monoclonal antibodies (MAbs) that antagonize toxin activity in vitro and protecting experimental animals against lethal S. aureus infection. [less ▲]

Les problèmes cutanés liés aux médicaments

Conference (2013, February 25)

Applications de l’échographie à la reproduction des ruminants

Learning material (2013)

Cette présentatiion illustre les prerequis physiques et anatomiques nécessaires à la compréhension des champs d'application de l'échographie en reproduction bovine.

Interplay between KLF4 and ZEB2/SIP1 in the regulation of E-cadherin expression.

in Biochemical and Biophysical Research Communications (2013), 431(4), 652

E-cadherin expression is repressed by ZEB2/SIP1 while it is induced by KLF4. Independent data from the literature indicate that these two transcription factors could bind close to each other in the ... [more ▼]

E-cadherin expression is repressed by ZEB2/SIP1 while it is induced by KLF4. Independent data from the literature indicate that these two transcription factors could bind close to each other in the proximal region of the E-cadherin gene promoter. We have here explored a potential competition between ZEB2 and KLF4 for the binding to the E-cadherin promoter. We show an inverse correlation between ZEB2 expression levels and KLF4 recruitment on the E-cadherin promoter in three breast cancer cell lines and in A431/HA.ZEB2 cells in which ZEB2 expression is induced by doxycycline (DOX). We identified a region of the E-cadherin promoter bound by KLF4 which is necessary for the activation of the E-cadherin promoter activity after KLF4 overexpression. This region is localized between positions -28 and -10 and thus overlaps with one of the ZEB2 binding sites. Deleting the bipartite ZEB2 binding site results in increased KLF4 induced E-cadherin promoter activity. Taken together, our results suggest that E-cadherin expression in cancer cells is controlled by a balance between ZEB2 and KLF4 expression levels. [less ▲]

Débats européens sur les formes de la vie marine au Siècle des Lumières

Conference (2013, February 22)

An occupational therapist as a new professional at home: which profiles of clients are reached ?

Poster (2013, February 22)

Quantitative gait assessment using an accelerometer technology as a predictive tool of falls among nursing home residents: a 6-month prospective study

in Proceedings of the meeting (2013, February 22)

Group size of a permanent large group of agile mangabeys (Cercocebus agilis) at Bai Hokou, Central African Republic

in Folia Primatologica : International Journal of Primatology = Internationale Zeitschrift für Primatologie = Journal international de Primatologie (2013), 84