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See detailMultidisciplinary approaches and fractionations to study lower termite symbiotic system and ligno-cellulose digestion
Bauwens, Julien ULg; Brasseur, Catherine ULg; Tarayre, Cédric ULg et al

Poster (2014, August)

Wood-feeding termites are a considerable source of enzymes active on ligno-cellulosic compounds. These enzymes are produced by the termite host and some representatives of its symbiotic microbial ... [more ▼]

Wood-feeding termites are a considerable source of enzymes active on ligno-cellulosic compounds. These enzymes are produced by the termite host and some representatives of its symbiotic microbial community, and are of particular interest in regard second generation biofuel. However, the complexity of microbial interactions renders micro-organisms isolation very difficult. Culture-independent methods permitted to gather a large amount of data and to understand a little more the role of each microbial population, particularly the prokaryotes. Proteomics allows working on the final product of gene expression, and corresponds more to the real operation of the digestive system. In order to investigate such a complex system, it is necessary to use multidisciplinary approaches and to fractionate this system. Zymography or affinity chromatography are used in parallel of routine proteomics techniques such as two-dimensional gel electrophoresis associated to MALDI-TOF mass spectrometry and nano-LC ESI-MS/MS. We used an artificial-diet based rearing to induce changes in microbial population balance. We performed preliminary assay to investigate the glycosylated proteome in the hindgut of a lower termite, using Multi-Lectin Affinity Chromatography (M-LAC) and enzymatic activity of harvested fractions was assessed on cellulosic substrates. [less ▲]

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See detailReliabilität und Validität des "Voice Handicap Index (VHI) adaptiert an die Gesangsstimme"
Morsomme, Dominique ULg; Barsties, B.; Kropp, J. et al

in Laryngo Rhino Otologie (2014)

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See detailPhytosanitary treatment of European pallets by microwave: developing a program to ensure compliance with ISPM 15 and monitoring its efficacy on the house longhorn beetle (Hylotrupes bajulus L.)
Henin, Jean-Marc; Leyman, Michael; Bauduin, Aline et al

in European Journal of Wood and Wood Products (2014)

Microwave heating was recently approved by the FAO as a significantly effective phytosanitary treatment for wood packaging material. According to ISPM 15 (FAO 2009), the target organisms are eradicated if ... [more ▼]

Microwave heating was recently approved by the FAO as a significantly effective phytosanitary treatment for wood packaging material. According to ISPM 15 (FAO 2009), the target organisms are eradicated if a temperature higher than 60 °C is maintained for 60 s across the entire profile of the board (i.e. 60 °C/60 s). A study using pallet boards was carried out in order to set up a treatment program that would meet ISPM 15 requirements in terms of wood temperature and insect mortality. A 4 m-long industrial tunnel oven (maximum power of 28.8 kW) was used to carry out the experiments. Temperature was measured by means of a VarioCAM® infrared camera. The most relevant results we found were: (i) achieving a temperature of 63.2 °C (Populus sp.) or 64.8 °C (Pinus sylvestris L.) on the upper surface of 22 mm-thick boards enabled compliance with FAO requirements (i.e. 60 °C/60 s), whatever the moisture content, basic density and initial temperature of the wood (provided the latter exceeded 0 °C); (ii) larvae >150 mg represented the most microwave-resistant life stage of Hylotrupes bajulus L.; (iii) the mortality rate of the larvae was influenced by the moisture content of the boards. Using the Gompertz model, we estimated the upper surface temperature that would be needed to achieve a 99.99683% mortality rate (the Probit 9 mortality level of efficacy) for the most microwave-resistant life stage of H. bajulus. This temperature was estimated to be 46.8 °C and 57 °C for wood with >50 and <25% moisture content, respectively. [less ▲]

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See detailComparison of patient's voice quality for T1A glottis carcinoma after endoscopic laser surgery or radiotherapy
Poncelet, Mélanie; DEMEZ, Pierre ULg; Moreau, Pierre ULg et al

Conference (2014, July 30)

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See detailAlgorithmic and Computational Advances for Fast Power System Dynamic Simulations
Aristidou, Petros ULg; Van Cutsem, Thierry ULg

in Proceedings of the IEEE PES General Meeting (2014, July 30)

In this paper, some algorithmic and computational advances are presented for power system dynamic simulations. The heart is a Schur-complement-based solution algorithm, stemming from domain decomposition ... [more ▼]

In this paper, some algorithmic and computational advances are presented for power system dynamic simulations. The heart is a Schur-complement-based solution algorithm, stemming from domain decomposition methods, applied to the differential-algebraic equation model. This algorithm is then accelerated computationally, by employing parallel computing techniques, and numerically, by exploiting time-scale decomposition and localization. Models of a real medium-scale system and a realistic large-scale test system are used for the performance evaluation of the proposed methods. [less ▲]

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See detailSound production to electric discharge: sonic muscle evolution in progress in Synodontis spp. catfishes (Mochokidae)
Boyle, Kelly; Colleye, Orphal ULg; Parmentier, Eric ULg

in Proceedings of the Royal Society B : Biological Sciences (2014), 281

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See detailNeoadjuvant chemotherapy in breast cancer induces miR-34a and miR-122 expression
FRERES, Pierre ULg; JOSSE, Claire ULg; Bovy, Nicolas ULg et al

in Journal of Cellular Physiology (2014)

Circulating microRNAs (miRNAs) have been extensively studied in cancer as biomarkers but little is known regarding the influence of anti-cancer drugs on their expression levels. In this article, we ... [more ▼]

Circulating microRNAs (miRNAs) have been extensively studied in cancer as biomarkers but little is known regarding the influence of anti-cancer drugs on their expression levels. In this article, we describe the modifications of circulating miRNAs profile after neoadjuvant chemotherapy (NAC) for breast cancer. The expression of 188 circulating miRNAs was assessed in the plasma of 25 patients before and after NAC by RT-qPCR. Two miRNAs, miR- 34a and miR-122, that were significantly increased after NAC, were measured in tumor tissue before and after chemotherapy in 7 patients with pathological partial response (pPR) to NAC. These 2 chemotherapy-induced miRNAs were further studied in the plasma of 22 patients with adjuvant chemotherapy (AC) as well as in 12 patients who did not receive any chemotherapy. Twenty-five plasma miRNAs were modified by NAC. Among these miRNAs, miR-34a and miR-122 were highly upregulated, notably in pPR patients with aggressive breast cancer. Furthermore, miR-34a level was elevated in the remaining tumor tissue after NAC treatment. Studying the kinetics of circulating miR-34a and miR-122 expression during NAC revealed that their levels were especially increased after anthracycline-based chemotherapy. Comparisons of the plasma miRNA profiles after NAC and AC suggested that chemotherapy-induced miRNAs originated from both tumoral and non-tumoral compartments. This study is the first to demonstrate that NAC specifically induces miRNA expression in plasma and tumor tissue, which might be involved in the anti-tumor effects of chemotherapy in breast cancer patients. [less ▲]

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See detailNew carbohydrate-active enzymes identified by screening two metagenomic libraries derived from the soil of a winter wheat field
Stroobants, Aurore ULg; Portetelle, Daniel ULg; Vandenbol, Micheline ULg

in Journal of Applied Microbiology (2014)

Aims Soils are rich, diversified environments where β-glucosidases abound because of their importance in organic matter degradation. The aim of this work was to discover new β-glucosidases by constructing ... [more ▼]

Aims Soils are rich, diversified environments where β-glucosidases abound because of their importance in organic matter degradation. The aim of this work was to discover new β-glucosidases by constructing two metagenomic DNA libraries from soil samples collected in winter and spring from a field of winter wheat. Methods and Results Both libraries were screened on esculin-supplemented medium so as to isolate candidates showing β-glucosidase activity. Candidate analysis revealed seven putative β-glycosidases and two putative glycosyltransferases, displaying 25 to 82% identity to known enzymes. The putative β-glycosidases belong to families GH1, GH3 and GH20 and the two putative glycosyltransferases, probably, to new families. In characterization tests performed on bacteria in suspension or spread on agar plates, some candidates appeared to hydrolyse several natural and synthetic substrates. These tests also highlighted interesting industrial characteristics, such as the activity of four β-glycosidases under alkaline conditions and the esculin-hydrolysing activity of a β-glucosidase candidate in the presence of glucose. Conclusions Seven putative β-glycosidases and two putative glycosyltransferases were found by functional screening of two metagenomic DNA libraries derived from agricultural soil. Significance and Impact of the Study This study has identified β-glycosidases and putative glycosyltransferases that have or may have interesting industrial characteristics. [less ▲]

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See detailJamming Signal Immunity Tests on GSM-R Communications Compared to EMC Basic Standards
Beauvois, Véronique ULg; Fontana, Michele; Canavero, Flavio et al

Conference (2014, July 29)

This paper presents the work being done in SECRET project (European Program FP7/2007-2013 under grant agreement number “285136”) to strengthen the European rail network against the potential jamming of ... [more ▼]

This paper presents the work being done in SECRET project (European Program FP7/2007-2013 under grant agreement number “285136”) to strengthen the European rail network against the potential jamming of the railway communications. Standardized immunity tests, currently conducted on communication equipment in the presence of electromagnetic (EM) interferences are presented. It then presents the tests carried out in SECRET to assess the resistance of railway communication systems face the signals generated by telecommunication jammers. [less ▲]

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See detailPhotographier le travail, représenter le futur. Iconographie du chantier des Halles à Paris
Dondero, Maria Giulia ULg

in Communication et Langages (2014), 180

This article investigates the semiotic strategies of visual representation in the urban space, as exemplified by the renovation project of the Forum des Halles in Paris. The corpus of images, taken from ... [more ▼]

This article investigates the semiotic strategies of visual representation in the urban space, as exemplified by the renovation project of the Forum des Halles in Paris. The corpus of images, taken from the brochure of the construction site and from the website www.parisleshalles.fr, includes photographic documentation and illustrations of the future buildings. The analysis of the visual enunciation marks out the relative simulacra of the producers and consumers of these images, and a semiotic study of the modes of representation of the buildings (virtualisation, actualisation, realisation, etc.) helps understanding how the present and the future are articulated in the iconography of a construction work in progress. [less ▲]

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See detailEuropean Union Public Diplomacy through the EEAS: the cases of Argentina, Brazil and Mexico
Azpíroz Manero, María Luisa ULg

Conference (2014, July 25)

Public diplomacy is a promising activity for international political actors to promote a positive image abroad. It also provides opportunities for dialogue, understanding and mutual benefit when ... [more ▼]

Public diplomacy is a promising activity for international political actors to promote a positive image abroad. It also provides opportunities for dialogue, understanding and mutual benefit when communicating and addressing challenges. Within the European Union (EU), the Lisbon Treaty and the European External Action Service (EEAS) have given a new strength to foreign policy. The EEAS, launched in December 2010, is the EU's main institution for public diplomacy. Argentina, Brazil and Mexico are relevant Latin American partners for the EU because of their size, population and political and economic potency in the region. This paper aims to answer the following questions: What is the context of EU relations with Argentina, Brazil and Mexico during the period of study (2011-2013)? What have been the EU's foreign policy and strategies towards these countries? How have public diplomacy and the work of the EEAS in these countries supported the objectives and strategies? This research includes information gained through the consultation of publications, official documents and interviews with personnel from the EEAS, both in Brussels and in the EU delegations in Latin America. [less ▲]

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See detailStreamable Laguerre-Voronoi Tessellation Model for Tomographic Images
Leblanc, Christophe ULg; Nguyen, Van Dung ULg; Wan, Fangyi et al

Conference (2014, July 25)

Introduction. Nowadays, the interest in foam materials is growing in several engineering fields [1]. Foams can exhibit a nonlinear mechanical behavior [2], which is highly depen- dent on their ... [more ▼]

Introduction. Nowadays, the interest in foam materials is growing in several engineering fields [1]. Foams can exhibit a nonlinear mechanical behavior [2], which is highly depen- dent on their microstructure [3]. Thus designing foams with specific mechanical properties can be very challenging. The present contribution is part of the ARC-Bridging project [4], whose objective is to predict the mechanical behavior of complex microstructured mate- rials via numerical simulations. A possible classification of foam models into two groups is: random models and deterministic models [5]. The random models frequently require statistical estimations of their parameters [6], whereas the deterministic models generally require numerically expensive image analyse. Indeed, classical analysis steps involve a distance tranform, a watershed and, optionally, a h-minima transform [5, p. 22], which can be computationally demanding [7, 8, 9]. Contribution. In the present Laguerre–Voronoi tessellation model, the image analysis part neither involes the watershed transform, nor the h-minima transform. Instead, fol- lowing the original idea of A.M. Lopez-Reina et E. Béchet [10], these two transforms are respectively replaced by a Hessian-based removal of spurious extrema and a clustering of the remaining maxima. This substitution allows the processing of large 3D-images by slices, i.e. “streaming”. The only limitation is enforced by the distance transform: the “feature” voxel of a given voxel should belong to the same slice. For foam images, this condition is fulfilled as long as the slice’s thickness is larger than the maximal foam cell’s size. Conclusion and perspectives. The aim of this contribution is to provide an efficient tessellation model for tomographic images of foams. From input tomographic images, this model provides a geometry model which will be used as an input for finite element simulations under the ARC-Briding project [4]. Simulation results will be compared with experimental measures. [less ▲]

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See detailSeasonal variability of meiofauna, especially harpacticoid copepods, in Posidonia oceanica macrophytodetritus accumulations
Mascart, Thibaud ULg; Lepoint, Gilles ULg; Deschoemaeker, Silke et al

in Journal of Sea Research (2014)

The overall aim of this study was (1) to assess the diversity and density of meiofauna taxa, especially harpacticoid copepod species, present within accumulated seagrass macrophytodetritus on unvegetated ... [more ▼]

The overall aim of this study was (1) to assess the diversity and density of meiofauna taxa, especially harpacticoid copepod species, present within accumulated seagrass macrophytodetritus on unvegetated sand patches and (2) to elucidate the community structure of detritus-associated harpacticoid copepods in relation to natural temporal variability of physico-chemical characteristics of accumulations. This was investigated in a Posidonia oceanica (L.) Delile seagrass ecosystem in the northwest Mediterranean Sea (Bay of Calvi, Corsica, 42°35’N, 8°43’E) using a triplicate macrophytodetritus core field sampling in two contrasting sites over the four seasons of 2011. Meiofauna higher taxa consisted of 50% Copepoda, which 87% belonged to the Harpacticoida order. Nematoda was the second most abundant taxa. The copepod community displayed a wide variety of morphologically similar and ecologically different species (i.e. mesopsammic, phytal, phytal-swimmers, planktonic and parasitic). The harpacticoid copepod community followed a strong seasonal pattern with highest abundances and species diversity in May-August, revealing a link with the leaf litter epiphyte primary production cycle. Aside from the important role in sheltering, housing and feeding potential of macrophytodetritus, a harpacticoid community BEST analysis demonstrated a positive correlation with habitat complexity and a negative correlation with water movements and P. oceanica leaf litter accumulation. [less ▲]

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See detailQuelle histoire pour les forêts d'Afrique centrale ?
Morin-Rivat, Julie ULg

Scientific conference (2014, July 24)

Conférence grand public sur les indicateurs de perturbations climatiques et anthropiques dans les forêts d'Afrique centrale.

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See detailGEOMETRICAL VALIDITY OF HIGH-ORDER PYRAMIDAL FINITE ELEMENTS
Johnen, Amaury ULg; Geuzaine, Christophe ULg

Conference (2014, July 24)

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See detailSur les quantifications équivariantes en supergéométrie de contact
Nibirantiza, Aboubacar ULg

Doctoral thesis (2014)

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See detailBrokers and intermediaries in environmental governance, Southern Sri Lanka
Rosillon, Caroline ULg

Conference (2014, July 23)

Since the end of the civil war in 2009, rural Southern Sri Lanka is facing growing tensions between fast economic development and the conservation of biodiversity, which contributes to the notoriety of ... [more ▼]

Since the end of the civil war in 2009, rural Southern Sri Lanka is facing growing tensions between fast economic development and the conservation of biodiversity, which contributes to the notoriety of the island. In order to preserve the green areas of the country, both the government and international institutions have made great efforts to implement projects combining biodiversity protection and poverty reduction as their objectives. Within that context, this paper focuses on a specific category of stakeholders, emerging from the implementation of the aforementioned projects: a wide range of intermediaries between local communities and the State, or international institutions. Based on an ethnographic study of two protected areas in Southern Sri Lanka, the paper will examine the role of those intermediaries in the implementation of environmental governance models and ideologies in local arenas. I will also analyze the complex relationships between those brokers and other categories of individuals or groups within the community: people living in protected areas, traditional figures of power, social and political elites, etc. Using fieldwork illustrations, I will stress how the emergence of brokers and intermediaries in the context of nature conservation may lead to the reorganization of social and political relationships at the local scale. [less ▲]

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