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See detailSibylle Lewitscharoff, Apostoloff
Houscheid, Karin ULg

Article for general public (2016)

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See detailEtude de cas, cathédrale de Beauvais (Oise), histoire de la construction et datations grâce à l'archéologie du bois
Hoffsummer, Patrick ULg

in Monumental : Revue Scientifique et Technique des Monuments Historiques (2016), 2016 1

L'archéologie des charpentes de la cathédrale de Beauvais combinée à la dendrochronologie et aux analyses de la couverture en plomb a largement contribué à l'enrichissement des connaissances à propos de l ... [more ▼]

L'archéologie des charpentes de la cathédrale de Beauvais combinée à la dendrochronologie et aux analyses de la couverture en plomb a largement contribué à l'enrichissement des connaissances à propos de l'histoire mouvementée du plus haut chœur gothique. [less ▲]

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See detailUn outil e-Health pour tous ? Le cas de l'informatisation du dossier infirmier au sein d'un hôpital belge
Slomian, Cynthia ULg; Schoenaers, Frédéric ULg

in Lovis, Christian; Séroussi, Brigitte; Ugon, Adrien (Eds.) et al eSanté pour tous (2016, June)

Contexte. Les soins de santé en Belgique et ailleurs sont confrontés à deux réalités : l’augmentation de la demande de soins, d’une part, et la volonté de l’Etat de diminuer les dépenses de santé, d’autre ... [more ▼]

Contexte. Les soins de santé en Belgique et ailleurs sont confrontés à deux réalités : l’augmentation de la demande de soins, d’une part, et la volonté de l’Etat de diminuer les dépenses de santé, d’autre part. Dans ce contexte, l’e-Health est souvent présentée comme la solution qui permettra de rationaliser les moyens, mais également d’augmenter l’efficience du système. Cet article se propose d’étudier une facette de l’e-Health : l’informatisation du dossier infirmier au sein d’un hôpital belge. Méthodes. Le matériau empirique rassemble des entretiens semi-directifs (n=30), des heures d’observation du travail infirmier (n=25) ainsi que l’étude de documents organisationnels. L’analyse du matériau repose sur la sociologie de l’action organisée. Résultats. L’introduction de l’ordinateur provoque des changements, mais également la persistance de routines organisationnelles. Discussion. Les infirmières s’approprient le dossier infirmier informatisé et tentent de maximiser leur pouvoir au sein de l’organisation. Conclusion. Un outil informatique n’est jamais totalement contraignant. Les acteurs se saisissent des marges de manœuvre laissée par celui-ci pour maximiser leur pouvoir. L’appropriation contingente du dispositif est au cœur de cette démarche de changement. [less ▲]

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See detailLaboratory identification of anaerobic bacteria isolated on Clostridium difficile selective medium
Rodriguez Diaz, Cristina ULg; Warszawski, Nathalie; Korsak Koulagenko, Nicolas ULg et al

in Acta Microbiologica et Immunologica Hungarica (2016)

Despite increasing interest in the bacterium, the methodology for Clostridium difficile recovery has not yet been standardised. Cycloserine cefoxitin fructose taurocholate (CCFT) has historically been the ... [more ▼]

Despite increasing interest in the bacterium, the methodology for Clostridium difficile recovery has not yet been standardised. Cycloserine cefoxitin fructose taurocholate (CCFT) has historically been the most used medium for C. difficile isolation from human, animal, environmental and food samples, and presumptive identification is usually based on colony morphologies. However, CCFT is not totally selective. This study describes the recovery of 24 bacteria species belonging to 10 different genera other than C. difficile, present in the environment and foods of a retirement establishment that were not inhibited in the C. difficile selective medium. These findings provide insight for further environmental and food studies as well as for isolation of C. difficile on supplemented CCFT. [less ▲]

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See detailUpdating Failure Probability Of A Welded Joint In Offshore Wind Turbine Substructures
Mai, Anh Quang ULg; Sørensen, John D.; Rigo, Philippe ULg

in The 35th International Conference on Ocean, Offshore and Arctic Engineering (OMAE) (2016, June)

For offshore wind turbines, the support structure contributes with a significant part to the Levelized Cost Of Energy (LCOE). LCOE is the total cost to build and operate a whole offshore wind turbine ... [more ▼]

For offshore wind turbines, the support structure contributes with a significant part to the Levelized Cost Of Energy (LCOE). LCOE is the total cost to build and operate a whole offshore wind turbine structure over its lifetime divided by the total energy output of the wind turbine over that lifetime. That total expected cost of operation & maintenance may be lowered by application of reliability- and risk-based maintenance strategies and updating of the reliability based on e.g. inspections performed during the design lifetime. Updating the reliability (or alternatively the failure probability) of a welded joint can theoretically be done using Bayesian updating. However, for tubular joints in offshore wind turbine substructure when considering a two dimensional crack growth and a failure criterion combined brittle fracture and material strength, the updating is quite complex due to the wind turbine loading obtained during operation. This paper considers how the reliability (or the probability of failure) of welded steel details can be updated in the case where the fatigue failure is modelled by a fracture mechanics approach and a Failure Assessment Diagram (FAD) is used to define a limit state equation. Besides, it is shown how the probability of failure of fatigue critical joints can be updated for various inspection scenarios. A two dimensional bi-linear model is considered for the crack growth. Calculation of the crack depth and the crack length are coupled. The stress intensity factor is calculated following the sophisticated procedure in BS 7910:2005. The initial crack size, the yield and ultimate strengths of steel, the fracture toughness, the stress intensity factor, and the stress-ranges are considered as uncertain and modelled by random variables. The probability of detection is used to account for uncertainty in crack inspections. By using Monte Carlo simulations, stress-range histories are generated randomly and together with the calculated stress intensity factors used to check the limit state condition using the FAD approach. The probability of failure updating is done for three inspection scenarios: No crack detected; Crack detected and repaired; Crack detected but not repaired. The paper presents illustrative results for offshore wind applications for the three scenarios, including comparisons of application of the FAD approach for modelling the limit state equation with a ‘conventional’ approach where it is assumed that failure happens when crack-depth reaches the plate thickness or the stress intensity factor is equal to or larger than the fracture toughness of the material. Finally, it is discussed how application of the updating procedure can be used for inspection planning for offshore wind turbine support structures, and thus also for reducing the required safety factors at the design stage. [less ▲]

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See detailPLANET TOPERS: Planets, Tracing the Transfer, Origin, Preservation, and Evolution of their ReservoirS
Dehant, Véronique ULg; Kabamba Baludikay, Blaise ULg; Beghin, Jérémie ULg et al

in Origins of Life & Evolution of the Biosphere (2016), DOI 10.1007/s11084-016-9488

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See detailInfluence of the inter‐stage coupling flexibility on the dynamics of multi‐stage rotors
Nyssen, Florence ULg; Golinval, Jean-Claude ULg

in Proceedings of the ASME Turbo Expo 2016 (2016, June)

In this work, a characterization of the inter-stage coupling in a one-piece multi-stage bladed structure is performed. More particularly, the effect of the inter-stage coupling flexibility on the mode ... [more ▼]

In this work, a characterization of the inter-stage coupling in a one-piece multi-stage bladed structure is performed. More particularly, the effect of the inter-stage coupling flexibility on the mode-shapes is evaluated. To this purpose, the MAC matrix between the mono-stage and multi-stage modes is computed for different drum Young’s modulus of the inter-stage coupling. In parallel, the strain energy located in the blades and in the connecting structure is computed for different levels of inter-stage coupling. This enables to establish a criterion to determine when a multi-stage finite element analysis is necessary instead of only computing the different mono-stage models separately. This criterion is based on the localization of the energy in the structure. Numerical analyses are performed on a two-stages academic bladed structure. [less ▲]

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See detailShedding Light on the Dark Side of Customer Participation: Investigating Customer Participation Stress
Treger, Stephanie; Büttgen, Marion; Schumann, Jan Hendrik et al

Conference (2016, June)

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See detailL'importance de l'appartenance ethnique en RDC
Kabamba, Bob ULg

Scientific conference (2016, June)

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See detailDefects do Catalysis: CO Monolayer Oxidation and Oxygen Reduction Reaction on Hollow PtNi/C Nanoparticles
Dubau, Laetitia; Nelayah, Jaysen; Moldovan, Simona et al

in ACS Catalysis (2016)

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See detailStein's method, many interacting worlds and quantum mechanics
McKeague, Ian; Peköz, Erol; Swan, Yvik ULg

E-print/Working paper (2016)

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See detailShedding Light on the Dark Side of Customer Participation: Investigating Customer Participation Stress
Treger, Stephanie; Büttgen, Marion; Schumann, Jan Hendrik et al

Conference (2016, June)

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See detailUnderstanding Usage Behaviour in a Peer-to-Peer Task Community
Deliège, Fanny ULg; Ates, Zelal ULg; Benoit, Sabine et al

Conference (2016, June)

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See detailCareer development for international researchers
Halleux, Isabelle ULg

Diverse speeche and writing (2016)

This talk as invited speaker aims to express how and why the stakeholders opinions converge on the competences required from researchers and the needs for their career assessment. Inspiring tools and very ... [more ▼]

This talk as invited speaker aims to express how and why the stakeholders opinions converge on the competences required from researchers and the needs for their career assessment. Inspiring tools and very good practices exist that be shared to help employers as well as the researchers themselves. [less ▲]

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See detailDental ceramics: classification and indications
Mainjot, Amélie ULg

in Esthetics in Dentistry (2016)

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See detailMyoclonin-1 modulates the post-translational modification of microtubules
Medard, Laurie ULg; Coumans, Bernard ULg; Lakaye, Bernard ULg et al

Poster (2016, June)

Juvenile myoclonic epilepsy is one of the most common forms of generalized genetic epilepsy. Genetic studies have shown that heterozygous mutations in Myoclonin1 are responsible for 3-9% of clinical cases ... [more ▼]

Juvenile myoclonic epilepsy is one of the most common forms of generalized genetic epilepsy. Genetic studies have shown that heterozygous mutations in Myoclonin1 are responsible for 3-9% of clinical cases worldwide. This protein contains three DM10 domains of unknown function and an EF-hand domain. We have previously demonstrated that Myoclonin1 is a microtubule-associated protein involved in cell division and radial migration during neocortex development. In cells, this protein co-localized with specific structures rich in microtubules (MTs) such as the centrosome, the poles of the mitotic spindle or the motile cilia but not with cytoplasmic MTs. This suggests post-translational modifications (PTM) of MTs may be important for the interaction between Myoclonin1 and MTs. We have co-express the different enzymes catalyzing PTM of MTs with Myoclonin1 in U2OS cell line. With one of these enzymes, we observed a strong increase in PTM in the presence of Myoclonin-1. This suggests that Myoclonin1 may interact with and modulate the activity of this enzyme. By using luciferase complementation assay and pull down experiments, we could demonstrate that it is indeed the case. Interestingly, the effect is observed even when a DM10 domain alone is co-expressed with the enzyme, suggesting for the first time a role for this domain. In conclusion our data suggest myoclonin-1 modulates specific PTM of MTs. This is of prime importance for microtubule dynamic and notably for neuroblast precursor migration during neocortex development. This could be the mechanism that explains why pathological forms of myoclonin-1 affect brain development. [less ▲]

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See detailGuidance Document on the Estimation of LOD and LOQ for Measurements in the Field of Contaminants in Food and Feed
Wenzl, Thomas; Haedrich, Johannes; Schaechtele, Alexander et al

Report (2016)

The European Union Reference Laboratory (EURL) for Polycyclic Aromatic Hydrocarbons (EURL PAH), the EURL for Heavy Metals in Feed and Food (EURL HM), the EURL for Mycotoxins (EURL Mycotoxins), and EURL ... [more ▼]

The European Union Reference Laboratory (EURL) for Polycyclic Aromatic Hydrocarbons (EURL PAH), the EURL for Heavy Metals in Feed and Food (EURL HM), the EURL for Mycotoxins (EURL Mycotoxins), and EURL for Dioxins and PCBs in Feed and Food aim to provide with this document guidance to official food control in the EU on the estimation of the limit of quantification of analytical methods for the determination of individual substances in the field of contaminants in feed and food. The document focusses on estimation of the limit of detection (LOD) and/or limit of quantification (LOQ) of polycyclic aromatic hydrocarbons (PAHs), heavy metals (HM), mycotoxins, and polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and polychlorinated biphenyls (PCBs). The concept presented here consists of two major routes: The first route responds to the requirements for determination of PAHs, HMs and Mycotoxins. The second route corresponds to PCDD/F and PCB analysis, where results are calculated as sumparameters and expressed in toxic equivalents (TEQs), converting thereby congener concentrations together with estimated LOQs using toxic equivalency factor (TEFs) into TEQs. Due to the nature of this more complex procedure, emphasis is put in the area of PCDD/F and PCB analysis on the LOQs, while LODs are of minor consequence. Terminology in this guidance document was adapted to the chemical analysis of contaminants in feed and food; hence, some of the general terms defined and used in international standards were replaced by more specific terms applicable to analytical chemistry. The presented statistical-mathematical approach is based on elements taken mainly from DIN 32645:2008-11 (DIN 2008) and ISO 11843-2:2000 (ISO 2000). This document covers only quantitative methods of analysis. The authors believe that LOD and LOQ values derived from the application of the presented experimental methodologies converge to a certain degree. Estimation of LOD/LOQ values based on blank measurements, and from calibration data is described. Signal-to-noise ratios are applied in the area of PCDD/Fs and PCBs. Mathematical terms and statistical background are presented as well. This guide document shall be applied systematically if measurement results are used for monitoring purposes and exposure modelling. However, authors are aware that precise knowledge of LOD/LOQ may not be required when assessing compliance with maximum levels exceeding LOD/LOQ. This guidance document shall be applied if maximum levels and analysis results are close to the expected LOQ. The authors acknowledge that the presented approach has its limitations and may not be generally applicable to all cases. [less ▲]

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See detail“The Sweet Smell of Chemistry” - Characterization of volatile mixtures from Life Sciences
Stefanuto, Pierre-Hugues ULg

Doctoral thesis (2016)

From a Separation Science standpoint, Life Sciences represent a broad source of complex compound mixtures that require powerful analytical strategies to be considered when characterization is aimed. The ... [more ▼]

From a Separation Science standpoint, Life Sciences represent a broad source of complex compound mixtures that require powerful analytical strategies to be considered when characterization is aimed. The depiction of the complex volatile organic compound (VOC) component of these mixtures is an important part of such characterization. The aim of this doctoral work was to develop a versatile analytical approach to resolve such VOC samples. For the characterization of complex VOC mixtures, comprehensive two-dimensional gas chromatography (GC×GC) is the method of choice. The combination of two GC separation columns offers higher peak capacity than single dimension GC (1D GC). Moreover, the hyphenation of GC×GC to a mass spectrometer provides an extra dimension of identification, especially when high-resolution (HR) time-of-flight mass spectrometers (TOFMS) is used. However, the optimization of the GC×GC conditions is not straightforward. Currently, there are no defined strategies to obtain the best capabilities these instruments, not only in terms of the optimization of the separation itself, but also mainly in terms of data processing efficiency. The first section of this work was devoted to the evaluation of theoretical aspects and the definition of a specific optimization strategy for GC×GC separation. The first part dealt with the heavily debated concept of ‘orthogonality’ was specifically investigated by means of a metric calculation, the Orthogonality Index (OI), linked to a new nomenclature describing the separation space usage. The second part was focused on the development of an optimization strategy for the sampling and the chromatographic separation of VOCs, as well as the processing of large sets of GC×GC data. This was conducted on complex beer aroma headspace replicates for sampling method selection, peak dispersion optimization, and robust multivariate statistical treatment. The third part of this theoretical investigation relied on the implementation of fast-GC conditions for GC×GC separation. The idea was to evaluate the effect of combining short columns, fast modulation, high temperature ramping, and high flow on GC×GC efficiency for explosive headspace analyses. In the second section, the focus was directed to challenging applications in the area of thanatochemistry, i.e. the chemistry of death, especially considering the volatile fraction of cadaveric decomposition, a complex chemical process releasing numerous VOCs. In this context, soil surrounding decomposing remains, headspace of cadavers, and more “specific” matrices (i.e. synthetic solutions and internal gas from decomposition) were analyzed by GC×GC TOFMS. Major attention was dedicated to the development of an analytical and data mining procedures for these complex VOC samples with emphasis on the study of the impact of different parameters on the cadaveric decomposition process. [less ▲]

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See detailNovel application assigned to toluquinol: inhibition of lymphangiogenesis by interfering with VEGF-C/VEGFR-3 signaling pathway.
Garcia-Caballero, Melissa; BLACHER, Silvia ULg; Paupert, J. et al

in British Journal of Pharmacology (2016), 173(12), 1966-87

BACKGROUND AND PURPOSE: Lymphangiogenesis is an important biological process associated with the pathogenesis of several diseases, including metastatic dissemination, graft rejection, lymphedema and other ... [more ▼]

BACKGROUND AND PURPOSE: Lymphangiogenesis is an important biological process associated with the pathogenesis of several diseases, including metastatic dissemination, graft rejection, lymphedema and other inflammatory disorders. The development of new drugs blocking lymphangiogenesis has become a promising therapeutic strategy. In this study, we aim at investigating the ability of toluquinol, a 2-methyl-hydroquinone isolated from the culture broth of the marine fungus Penicillium sp. HL-85-ALS5-R004, to inhibit lymphangiogenesis in vitro, ex vivo and in vivo. EXPERIMENTAL APPROACH: We used human lymphatic endothelial cells (LEC) to analyze the effect of toluquinol in 2D and 3D in vitro cultures, and in the ex vivo mouse lymphatic ring assay. For in vivo approaches, the transgenic Fli1:eGFPy1 zebrafish, the mouse ear sponges and cornea models were used. Western-blotting and apoptosis analyses were carried out to search for drug targets. KEY RESULTS: Toluquinol inhibited LEC proliferation, migration, tubulogenesis and sprouting of new lymphatic vessels. Furthermore, toluquinol induced LEC apoptosis after 14 h of treatment in vitro, blocked the thoracic duct development in zebrafish, and reduced the VEGF-C-induced lymphatic vessel formation and corneal neovascularization in mice. Mechanistically, we are providing evidence that this drug abrogates the VEGF-C-induced VEGFR-3 phosphorylation in a dose-dependent manner, and represses Akt and ERK1/2 phosphorylations. CONCLUSIONS AND IMPLICATIONS: Based on these findings, we propose toluquinol as a new candidate with pharmacological potential for the treatment of lymphangiogenesis-related pathologies. Notably, its ability to suppress corneal neovascularization paves the way for applications in vascular ocular pathologies. This article is protected by copyright. All rights reserved. [less ▲]

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