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See detailInfluence of terroir on the fungal assemblages associated to common bean seed
Klaedtke, Stephanie ULg; Jacques, Marie-Agnès; Chable, Véronique et al

Conference (2015, June 25)

Plants have evolved in association with microbial assemblages, also known as microbiota, which can affect plant growth and health. In contrast with other plant habitats, microbiota associated to seeds ... [more ▼]

Plants have evolved in association with microbial assemblages, also known as microbiota, which can affect plant growth and health. In contrast with other plant habitats, microbiota associated to seeds have only recently become an object of research. First surveys reveal that seed-associated microbial assemblages are composed of 50 to 1000 bacterial and fungal operational taxonomic units (OTUs) (Barret et al., 2015), including plant pathogens. Common bean (Phaseolus vulgaris L.) is affected by several seed-borne pathogens representing a phytosanitary challenge for common bean seed production. In a participatory research project (Farm Seed Opportunities, a European FP6 project), small scale organic seed producers emitted the hypothesis that seed microbiota in their integrity, including pathogens, play a role in shaping the crop as it adapts to local environments, leading to more resilience (Döring et al., 2014). As a first step to investigating this hypothesis, we analyzed 27 bean seed samples to assess whether the seed microbiota are indeed site-dependant or in contrary transmitted from on plant generation to the next within each cultivar. [less ▲]

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See detailIs western lowland gorilla a good gardener? Effect of fecal matrix and deposition at nest sites on seedling growth
Haurez, Barbara ULg; Brostaux, Yves ULg; Petre, Charles-Albert ULg et al

Conference (2015, June 25)

The Western lowland gorilla is a key dispersal agent, which disperses viable seeds of various size. In consequence of its nesting behavior, gorilla deposits half of its feces, and therefore about half of ... [more ▼]

The Western lowland gorilla is a key dispersal agent, which disperses viable seeds of various size. In consequence of its nesting behavior, gorilla deposits half of its feces, and therefore about half of dispersed seeds, at nest sites. This study was conducted in a logging concession of southeast Gabon where 78 % of the observed nest sites (n=183) were installed in open canopy terra firma forest. The aim of this research was to assess if seed deposition by gorillas (i) in fecal matrix and (ii) at nest sites is advantageous for seedling growth. To assess the effect of fecal matrix, seeds of Santiria trimera (Burseraceae), Chrysophyllum lacourtianum (Sapotaceae) and Plagiostyles africana (Euphorbiaceae) collected in gorilla feces were sown with and without fecal matrix in a nursery at the study site. The impact of seed deposition at nest sites on seedling growth was evaluated in situ. Seedlings of Santiria trimera and Dacryodes normandii (Burseraceae) were installed at nest sites and at closed canopy terra firma forest sites. For both type of trials, seedling growth was monitored. [less ▲]

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See detailDéveloppement et analyse d’un modèle in vitro d’infection épidermique par dermatophytes anthropophiles
Faway, E; Cambier, Ludivine ULg; Lambert de Rouvroit, C et al

in Proceedings du 33e Congrès Annuel de Recherche Dermatologique (CARD) (2015, June 25)

Detailed reference viewed: 22 (0 ULg)
See detailRobustifier nos analyses de données: un must!
Aerts, Stéphanie ULg

Conference given outside the academic context (2015)

Financiers, biologistes, psychologues, économistes ou chimistes,… tous utilisent des outils statistiques pour prédire, expliquer, modéliser, comprendre des processus spécifiques à leur domaine. Néanmoins ... [more ▼]

Financiers, biologistes, psychologues, économistes ou chimistes,… tous utilisent des outils statistiques pour prédire, expliquer, modéliser, comprendre des processus spécifiques à leur domaine. Néanmoins, les résultats de toute analyse statistique, de la plus simple à la plus complexe, peuvent être fortement influencés, voire renversés, par la présence d’une seule valeur atypique. S’il est assez aisé de surmonter ce problème pour de simples ensembles de données, lorsque plusieurs caractéristiques sont mesurées simultanément, disposer de méthodes robustes nécessite l’emploi d’algorithmes alliant à la puissance actuelle de nos ordinateurs, des théorèmes mathématiques parfois vieux de plus de 50 ans. A l’heure où on dispose de bases de données de plus en plus grandes mais de qualité inégale, il est indispensable que les praticiens se familiarisent avec les statistiques robustes afin de fiabiliser leurs conclusions. [less ▲]

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See detailThe microbiome from the Lyme disease principal reservoir host in southern Quebec (Peromyscus leucopus)
André, Adrien ULg; Mouton, Alice ULg; Millien, Virginie et al

Poster (2015, June 25)

The emergence of the Lyme disease in Southern Quebec appears directly linked to the recent arrival in the region of the rodent Peromyscus leucopus. Indeed, this species is considered to be the principal ... [more ▼]

The emergence of the Lyme disease in Southern Quebec appears directly linked to the recent arrival in the region of the rodent Peromyscus leucopus. Indeed, this species is considered to be the principal reservoir of the bacteria Borrelia Burgdorferei, responsible of the Lyme disease and the recent climatic warming has allowed the mice to colonize higher latitude territories. Other factors, like the regulation made by pathogens might as well play an important role in the dynamic of expansion of P. leucopus. In our project, we sampled several populations of P. leucopus from the North American border, where the species is thought to be present for 30 years, to the most recently colonized zones, situated approximatively 150km inside of the Quebec province. A characterisation of their microbiome was then performed from their liver, spleen and lungs. Our objectives are threefold: First, we settled a protocol based on NGS methods for the detection of Borrelia Burgdorferei in micro-mammal internal organs. Second, we plan to identify the Borreliosis infection zones and to study their spatio-temporal evolution. Third, we aim to test which of the central-marginal hypothesis or the enemy release hypothesis is best describing the scenario presently happening in southern Quebec concerning the distribution’s expansion of P. leucopus. These information will be of great interest to understand the dynamic of emergence of the Borreliosis and to predict the current and future distribution of this disease in order to inform the Canadian health authorities. [less ▲]

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See detail'En fonction du futur': la fonction publique régionale wallonne sous contrainte d'innovation
Claisse, Frédéric ULg; Guyot, Jean-Luc

Conference (2015, June 25)

Au départ d'une réflexion prospective entamée par l'IWEPS, la communication s'interroge sur les futurs possibles de la fonction publique régionale wallonne, considérée comme un environnement en ... [more ▼]

Au départ d'une réflexion prospective entamée par l'IWEPS, la communication s'interroge sur les futurs possibles de la fonction publique régionale wallonne, considérée comme un environnement en transformation soumis à de fortes pressions (budgétaires, institutionnelles, démographiques), mais aussi à des contraintes d'innovations multiples: recomposition du métier de fonctionnaire, modes de gestion à réinventer, e-government, concurrence de nouveaux offreurs de services au public, changements dans les attentes des usagers. [less ▲]

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See detailLes complications rénales du diabète
Krzesinski, Jean-Marie ULg

Conference (2015, June 24)

Detailed reference viewed: 34 (3 ULg)
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See detailGalectin-3 and suppression of tumorignenicity 2 measurment in participat at the "Tor des Géants"
LE GOFF, Caroline ULg

in Final programme European College of sport Science (2015)

Detailed reference viewed: 21 (4 ULg)
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See detailDUSP3 Phosphatase Deficiency or Inhibition Limits Platelet Activation and Arterial Thrombosis
Rahmouni, Souad ULg; MUSUMECI, Lucia ULg; Kuijpers, Marijke J et al

Conference (2015, June 24)

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See detailA hydrochemical - isotopic approach for assessing factors controlling the regional pollution of an urban aquifer
Gesels, Julie ULg; Orban, Philippe ULg; Popescu, Ileana-Cristina et al

Poster (2015, June 24)

The alluvial aquifer of the Meuse River is contaminated at regional scale in the urbanized and industrialized area of Liège in Belgium, in particular inorganics pollutants such as sulfate, nitrate and ... [more ▼]

The alluvial aquifer of the Meuse River is contaminated at regional scale in the urbanized and industrialized area of Liège in Belgium, in particular inorganics pollutants such as sulfate, nitrate and ammonium. The sources of those contaminants are numerous: brownfields, urban waste water, subsurface acid mine drainage from former coal mines, atmospheric deposits related to former pollutants emissions in the atmosphere ... Sulfate, nitrate and ammonium are both typical pollutants of the aquifer and tracers of the possible pollution sources. In the Water Framework Directive context, a detailed hydrogeochemical characterization of groundwater was performed. The aim is to determine the origin of the inorganic contaminations, the main processes contributing to poor groundwater quality and the spatial extent of the contaminations. A large hydrochemical sampling campaign was performed, based on 71 selected representative sampling locations, to better characterize the different vectors (end-members) of contamination of the alluvial aquifer and their respective contribution to groundwater contamination in the area. Groundwater samples were collected and analyzed for major and minor compounds and metallic trace elements. The analyses also include stable isotopes in water, sulfate, nitrate, ammonium, dissolved inorganic carbon, boron and strontium. Different hydrogeochemical approaches are combined to obtain a global understanding of the hydrogeochemical processes at regional scale. Hydrochemical interpretations are based on classical diagrams, spatial distribution maps, geochemical equations, multivariate statistics such as self-organizing maps and isotopic analyses. With this combined approach, the location of the contaminant sources and most contaminated sectors of the alluvial aquifer together with a better understanding of geochemical processes involved are obtained. Redox processes strongly influence the composition of groundwater, specifically for compounds degrading the quality of groundwater in the area (sulfate, nitrate and ammonium). The highest concentrations of sulfate can be associated with the post-mining stage in the acid mine drainage process. Various reactions involving nitrogen compounds have been identified and allow a better understanding of causes of high concentrations of ammonium and nitrate. Denitrification and sulphate reduction are also demonstrated based on isotopic ratios. [less ▲]

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See detailAging mechanisms in amorphous phase-change materials
Raty, Jean-Yves ULg

Conference (2015, June 23)

Aging phenomena are common to all amorphous structures, but of special importance in phase change materials (PCM) since it impedes the realization of multi-level memories. Different interpretations have ... [more ▼]

Aging phenomena are common to all amorphous structures, but of special importance in phase change materials (PCM) since it impedes the realization of multi-level memories. Different interpretations have been proposed, but we focus here on the structural relaxation of amorphous GeTe, chosen because it is the simplest system that is representative of the wider class of GST alloys, lying along the GeTe-Sb2Te3 composition line of the GeSbTe phase diagram. The direct melt-and-quench DFT based Molecular Dynamics approach leads to models with a few hundred atoms, and, hence a small number of atomic environments. Here we sample a large number of local atomic environments, and bonding schemes, by chemically substituting different alloys to favor different local atomic structures. This enables spanning a larger fraction of the configuration space relevant to aging. GST alloys are known to display complex bonding that does not follow the chemist’s “octet-rule”. This lead to many controversies, especially concerning the local structure around Ge atoms. We overcome this problem by using state of the art non local DFT-MD, including the so-called van der Waals corrections. This leads to more clearly defined environments that are thoroughly analyzed. We can then identify their fingerprints in the available structural experimental data and assess their stability to find the driving forces leading to the structural relaxation. The calculated electronic properties nicely match the most recent photothermal deflection spectroscopy experiments. Our results support a model of the amorphous phase and its time evolution that involves an evolution of the local (chemical) order towards that of the crystal (by getting rid of homopolar bonds), and an evolution of its electronic properties that drift away from those of the crystal, driven by an increase of the Peierls-like distortion of the local environments in the amorphous [1]. [1] J.Y Raty, W. Zhang, J. Luckas, C. Chen, R. Mazzarello, C. Bichara and M. Wuttig, Nature Comm. To appear. [less ▲]

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See detailDistributive contact lattices with nontangential part-of relations
Raskin, Julien ULg

Conference (2015, June 23)

In a Boolean algebra, the "contact" and "nontangential part-of" relations are linked and interdefinable thanks to the complement. These relations yield on the Stone dual of the algebra two closed ... [more ▼]

In a Boolean algebra, the "contact" and "nontangential part-of" relations are linked and interdefinable thanks to the complement. These relations yield on the Stone dual of the algebra two closed relations that appear to be the same. In a distributive lattice, the contact and nontangential part-of relations also yield dual closed relations on the Priestley dual. However, as the link between them is lost, these relations are not equal anymore. We explore the conditions linking the contact and nontangential part-of relations one should add in order to recover these relations knowing the intersection of their dual. [less ▲]

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See detailFULLY AUTOMATED ELECTROPHORETICALLY MEDIATED MICROANALYSIS SYSTEM FOR CYP1A1 ACTIVITY MONITORING
Farcas, Elena ULg; Servais, Anne-Catherine ULg; Lamalle, Caroline ULg et al

Poster (2015, June 23)

Introduction Since the efficacy and toxicity of drugs are closely related to their pharmacokinetics, a good understanding of metabolic pathways is important at an early stage of development. The ... [more ▼]

Introduction Since the efficacy and toxicity of drugs are closely related to their pharmacokinetics, a good understanding of metabolic pathways is important at an early stage of development. The identification of the enzymes involved in drug metabolism is thus of critical importance for the design of further clinical studies. The availability of specifically expressed human CYPs, namely supersomes, allows the investigation of the contribution of a single metabolic enzyme to the biotransformation pathway of the compound under investigation. CYP1A1, a member of the cytochrome P450 superfamily, was studied in this project. Interestingly, it has been described to be over expressed in various types of cancer. Consequently, CYP1A1 has emerged as a particularly interesting target for cancer therapy. Methods All the experiments were carried out on a HP3DCE system equipped with an on-column DAD. The EMMA procedure was performed by injecting a plug containing CYP1A1 supersomes, followed by a plug that contained the co-factor and the substrate, then another plug of CYP1A1 supersomes. The reaction was triggered by the application of a voltage switch. The voltage was then turned off to allow the metabolic reaction to occur. The separation of the components was then performed at -25 kV. Results The present study describes the development of a fully automatized in-capillary method to follow metabolization of 7-hydroxycoumarin and screen CYP1A1 inhibitors. After preliminary studies, satisfying results were obtained using CYP1A1 at a concentration of 200 pmol/mL, while the incubation time was settled to 15 min. Equal reactant plugs were injected at -50 mbar for 6 sec. The short-end injection performed gave rise to a baseline separation of the molecules (substrate, product, CYP1A1 and NADPH) in less than 2 minutes. Adequate plugs overlap was obtained using electrophoretic mixing. The DoE performed highlighted that the voltage switch has a great impact on the metabolite formation. The amount of product obtained in the optimal conditions was found to be comparable to the one detected after conventional off-line metabolization. Besides the interest of developing an automatized CE approach for metabolisation studies, we also wanted to investigate the potentiality of this approach to screen CYP1A1 inhibitors. The ability of our system to monitor CYP1A1 inhibition was undertaken with apigenin, a well-known inhibitor. It is noteworthy that the compatibility of our system with MEKC ensures its applicability to a large variety of molecules. Novel aspect Monitoring CYP1A1 activity using a rapid and fully automated EMMA method that could be used for new anticancer agents screening. [less ▲]

Detailed reference viewed: 63 (10 ULg)
See detailRoles and Challenges of Mutuals as Actors of the Social Economy: a Social Enterprise Perspective
Defourny, Jacques ULg

Conference given outside the academic context (2015)

Detailed reference viewed: 14 (1 ULg)