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See detailExpression study of FRD3 in Arabidopsis relatives
Scheepers, Maxime ULg; Charlier, Jean-Benoit ULg; Spielmann, Julien ULg et al

Poster (2015, June 08)

Transcriptomic studies identified genes which are constitutively over-expressed in A. halleri compared to A. thaliana and which may have a role in metal tolerance or accumulation (1-3). A candidate gene ... [more ▼]

Transcriptomic studies identified genes which are constitutively over-expressed in A. halleri compared to A. thaliana and which may have a role in metal tolerance or accumulation (1-3). A candidate gene encodes FRD3, a member of the MATE family of membrane transporters (56 members in A. thaliana). It is a citrate transporter involved in iron homeostasis (4-6) and playing a role in zinc tolerance in A. thaliana (7). We are aiming to analyse the FRD3 high expression in A. halleri and the FRD3 function in zinc and iron homeostasis in A. thaliana. [less ▲]

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See detailTowards the identification of mechanisms underlying the high expression of HMA4, an essential gene for zinc hyperaccumulation in Arabidopsis halleri.
Spielmann, Julien ULg; Nouet, Cécile ULg; Scheepers, Maxime ULg et al

Poster (2015, June 08)

To maintain metal homeostasis within physiological limits independently of metal concentrations present in soil, plants developed a complex network including metal uptake, chelation, trafficking, and ... [more ▼]

To maintain metal homeostasis within physiological limits independently of metal concentrations present in soil, plants developed a complex network including metal uptake, chelation, trafficking, and storage processes. In this network, transporters have a central role. To study the molecular basis of zinc homeostasis in plants, we are using A. halleri, a zinc- and cadmium-tolerant and zinc-hyperaccumulating species representing the extreme end of natural variation in terms of metal homeostasis. Indeed, A. halleri stores high amount of metals in vacuoles of above-ground tissues (>1% Zn or 0.01% Cd in shoot dry weight). Cross-species transcriptomic studies identified about thirty genes which are constitutively over-expressed in A. halleri compared to its non-tolerant and non-hyperaccumulator sister species, Arabidopsis thaliana (1-3). HMA4 is one of these genes. It encodes a Zn/Cd plasmamembrane pump involved in xylem loading/unloading which is a key step in the hyperaccumulation process (4-5). The HMA4 overexpression is determined by a combination of gene triplication and cis-regulatory changes increasing transcription levels of each three HMA4 copies (5). We aim to identify the cis-elements underlying high expression of HMA4 in A. halleri using promoter-reporter constructs. [less ▲]

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See detailA lysine cluster in domain II of Bacillus subtilis PBP4a plays a role in the membrane attachment of this C1 PBP.
Vanden Broeck, Arnaud; Dauvin, Marjorie ULg; Sauvage, Eric et al

Poster (2015, June 08)

Bacillus subtilis PBP4a belongs to the class-C1 PBPs characterized by two internal additional domains of unknown function. Seven lysine residues (K) are protruding from domain II. Four of them: K86, K114 ... [more ▼]

Bacillus subtilis PBP4a belongs to the class-C1 PBPs characterized by two internal additional domains of unknown function. Seven lysine residues (K) are protruding from domain II. Four of them: K86, K114, K119 and K265 have been mutated in glutamine residues (Q). Both proteins (WT and Mut4KQ PBP4a) have been produced without signal peptide in E. coli and their sub-cellular localizations determined by measuring the DD-carboxypeptidase activities in the different compartments (cytoplasmic vs membrane attached proteins). After purification, their binding to B. subtilis membranes has been compared: WT PBP4a interacts in vitro with membranes isolated from this organism in contrast to Mut4KQ PBP4a that remains entirely unbound. In absence of any amphiphilic peptide in PBP4a, the crown of positive charges on the surface of domain II is likely responsible for the PBP cellular localization in interaction with the cytoplasmic membrane. [less ▲]

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See detailFluorinated alcohols as activators for the solvent-free chemical fixation of carbon dioxide onto epoxides
Gennen, Sandro ULg; Alves, Margot ULg; Méreau, Raphaël et al

in ChemSusChem (2015), 8(11), 1845-1849

The addition of fluorinated alcohols to onium salts provides highly efficient organocatalysts for the chemical fixation of CO2 onto epoxides under mild experimental conditions. The combination of online ... [more ▼]

The addition of fluorinated alcohols to onium salts provides highly efficient organocatalysts for the chemical fixation of CO2 onto epoxides under mild experimental conditions. The combination of online kinetic studies, NMR titrations and DFT calculations allows understanding this synergistic effect that provides an active organocatalyst for CO2/epoxides coupling. [less ▲]

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See detailA new design of organic radical batteries (ORBs): carbon nanotube buckypaper electrode functionalized by electrografting
Aqil, Abdelhafid ULg; Vlad, Alexandru; Piedboeuf, Marie-Laure ULg et al

in Chemical Communications (2015), 51(45), 9301-9304

A novel hybridmaterial displaying a fast and reversible charge storage capability is prepared by electrografting of an alkoxyamine-bearing acrylate onto a carbon nanotube buckypaper, followed by the quan ... [more ▼]

A novel hybridmaterial displaying a fast and reversible charge storage capability is prepared by electrografting of an alkoxyamine-bearing acrylate onto a carbon nanotube buckypaper, followed by the quan- titative generation of an electroactive polynitroxide. [less ▲]

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See detailUNRAVELLING THE ROLES OF LYSINE ACETYLATION BY ELP3 DURING INNER EAR DEVELOPMENT
Mateo Sanchez, Susana ULg; Delacroix, Laurence ULg; Freeman, Stephen ULg et al

Poster (2015, June 06)

Given the importance of acetylation homeostasis in controlling developmental processes [1-3], we planned to investigate its role in inner ear formation and focused our attention on Elp3 acetyl-transferase ... [more ▼]

Given the importance of acetylation homeostasis in controlling developmental processes [1-3], we planned to investigate its role in inner ear formation and focused our attention on Elp3 acetyl-transferase, a member of the Elongator complex recently implicated in neurogenesis [4]. To determine the role of Elp3 in the inner ear, we first analysed the spatio-temporal pattern of ELp3 mRNA expression and showed that it was expressed in the entire early otocyst at E11.5 and persisted later in the sensory epithelium of the cochlea (the organ of Corti), in the spiral ganglion, in the stria vascularis and in the vestibule. To unravel in vivo functions of Elp3 in the inner ear, we used conditional knock-out mice in which Elp3 gene is deleted from early otocyst (Elp3 cKO). We submitted these mice to a battery of vestibular testing (i.e. stereotyped circling ambulation, head bobbing, retropulsion, and absence of reaching response in the tail-hanging test) and found significant abnormalities. Besides, the auditory brain stem response of Elp3 cKO indicated that these mice are severely deaf. At the cellular level, we did not find any structural abnormalities nor cell patterning defects that could explain deafness or balance dysfunction in Elp3 cKO mice. However, we detected some defaults in the planar orientation of their auditory hair cell bundle. We were also able to demonstrate an increased level of apoptosis in the Elp3 cKO spiral ganglion at E14.5 leading to a reduced number of neurons and fibers innervating the cochlear hair cells as well as a reduced number of their synaptic ribbons at P15. Moreover, the remaining spiral ganglion neurons extend processes showing clearly defects regarding hair cells innervation (misorientation of fibers). In conclusion, our results clearly show a role for Elp3 both in hearing and balance. We plan to go deeper in the mechanisms involved through the identification of the proteins that are targeted for acetylation by Elp3. [less ▲]

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See detailLa relation UE-CELAC. A la recherche d'un nouveau souffle
Santander, Sébastian ULg

Article for general public (2015)

Detailed reference viewed: 27 (10 ULg)
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See detailDesign and synthesis of PET-probes targeting AMPA-subtype receptors
Deverdenne, François ULg; Claes, Giselle ULg; Goffin, Eric ULg et al

Poster (2015, June 05)

The AMPA subtype of glutamatergic receptors is the main actor in the excitatory neurotransmission in the mammalian central nervous system. These receptors are involved in the expression and the ... [more ▼]

The AMPA subtype of glutamatergic receptors is the main actor in the excitatory neurotransmission in the mammalian central nervous system. These receptors are involved in the expression and the maintenance of the long-term potentiation, a phenomenon closely linked to cognitive and memorization processes. Based on experimental data collected in recent years, the use of AMPA potentiators seems to be an interesting approach in the treatment of cognitive deficits (e.g. Alzheimer disease), schizophrenia or depression. Such AMPA signal potentiation could be mediated by positive allosteric modulators (PAMs) of the AMPA receptors, a class of compounds able to produce a fine signal tuning in the presence of the endogenous ligand in the synapse, providing less toxicity than direct agonists. With this approach, the laboratory of Medicinal Chemistry of Liège university developed many series of AMPA potentiators , among which 1,2,4-benzothiadiazine 1,1-dioxides (BTDs). In order to better understand the in vivo mapping of AMPA receptors and its evolution in neurological diseases, the present work aims at developing the design and the synthesis of BTDs positive allosteric modulators radiolabeled with a fluorine-18 atom. Based on previously synthesized series in this field, we investigate the synthesis of a new class of high-affinity AMPA potentiators characterized by the presence of a fluorine atom at selected positions on the structure of the AMPA potentiators. Thanks to in vitro pharmacological evaluations, we will further determine the best candidates for their fluorine-18 radiolabeling. [less ▲]

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See detailStrategy for financing foundations: how do they create value?
Mernier, Amélie ULg

Conference (2015, June 05)

Traditionally, the research on strategy has rather focused on profit-seeking organizations whose primary objective is to generate revenues through the price mechanism. Nevertheless, all organizations ... [more ▼]

Traditionally, the research on strategy has rather focused on profit-seeking organizations whose primary objective is to generate revenues through the price mechanism. Nevertheless, all organizations benefit from developing a strategic approach (Moore 2000). This paper focuses on financing foundations, non-profit organizations that are private, non-membership based, self-governing and serving a public purpose (Anheier 2001) and that make grants, give loans or hold equity. The core activity of a financing foundation is to finance operating intermediaries, for example non-profit association, NGOs or social enterprises, which will then implement projects and programs directly in line with the mission of the financing foundation. During the last decades, these organizations have made a breakthrough by establishing themselves as private actors that will look after the public interest. For long, strategic thinking has been impeded in foundations because the essence of the philanthropic action was giving without any expectation in terms of social impact. Today, the legitimacy of these organizations is challenged and there is a stronger demand for efficiency reinforced by the emergence of a new philanthropy inspired by corporate management principles (e.g. venture philanthropy considered as the more advocated strategic approach (Gautier et Pache 2014) ) and the question of strategy really matters. This paper aims at designing a conceptual strategic framework for financing foundations whose ultimate goal is to create value for society. To justify the development of a specific strategic framework for financing foundations, I first compare financing foundations with other financing organization according to six dimensions: ultimate goal, sources of resources, nature of the contribution, means, logic of action and accountability. Then, I review the academic literature in the field of philanthropy having a strategy focus in order to highlight the current gaps in the literature. Only few authors give a comprehensive view of the strategic question for this type of organization. Existing papers focus only on fragments of strategy and tackle two strategic issues: the mission statement and the evaluation. The decision-making processes by which the initial objectives are achieved are ignored; the strategic choices which philanthropic organizations make to fulfill their mission have not yet been identified. Based on the identification of key strategic commitments (in terms of scope and mechanisms) underlying the implementation of the predefined social mission and two crucial trade-offs (impact expectation and level of mission objectivity), the strategic dimensions of a financing foundations, leading to value creation for the society, are conceptualized. Two processes of value creation are distinguished: direct and indirect value creation. The course of actions chosen by a financing foundation is closely connected to its ultimate goal that is, increase the utility of a target group of beneficiaries (i.e. direct value creation). In addition, even if the very objective of the financing foundation is not to increase the utility of its donors and founders, it is effectively what happens (i.e. indirect value creation). The empirical research that will be conducted based on the strategic conceptual framework is then described and additional research questions are highlighted. [less ▲]

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See detailStatistical analysis and multi-instrument overview of the quasi-periodic 1-hour pulsations in the Saturn's outer magnetosphere
Palmaerts, Benjamin ULg; Roussos, Elias; Krupp, Norbert et al

Conference (2015, June 05)

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See detailApports de l’analyse d’unités médiatrices sociocognitives tels que les schémas précoces inadaptés sur la compréhension et la prédiction de comportements d’agressions sexuelles.
Garcet, Serge ULg

Conference (2015, June 05)

Face à la complexité de la problématique de l’agression sexuelle, la compréhension du phénomène s’appuie pour certains modèles explicatifs sur l’agresseur sexuel et ses caractéristiques propres tant ... [more ▼]

Face à la complexité de la problématique de l’agression sexuelle, la compréhension du phénomène s’appuie pour certains modèles explicatifs sur l’agresseur sexuel et ses caractéristiques propres tant internes au niveau d’éventuels troubles de personnalité, de distorsions cognitives, de fantaisies imaginatives sous-jacentes notamment, qu’externes en envisageant par exemple l’environnement familial, l’adaptation sociale ou encore d’éventuels traumatismes précoces,... D’autres modèles encore proposent une analyse catégorielle basée sur une lecture criminologique structurée autour des modes d’agressions et des caractéristiques de l’acte (violence, désinhibition,…). Ces différentes caractéristiques sont régulièrement envisagées conjointement lors de l’évaluation en expertise, d’une éventuelle dangerosité et du risque de récidive mais trop souvent au travers d’une lecture statique des interactions. Il est cependant maintenant scientifiquement bien établi que nos comportements résultent d’un déterminisme réciproque entre nos actions, nos processus de traitements sociocognitifs et notre environnement. Il est également bien étayé dans la littérature que ce que nous appelons « la personnalité » implique différents mécanismes autoréflexifs et autorégulateurs qui déterminent autant notre façon de nous représenter le monde qui nous entoure que nos façons d’interagir au travers de nos comportements. C’est pourquoi il nous paraît important de prendre en compte ces unités médiatrices de traitement sociocognitif lorsque nous sommes confrontés à l’évaluation d’agresseurs sexuels. Dans une perspective théorique de compréhension des mécanismes sous-jacents aux actes d’agression sexuelle et expertale autant que clinique d’appréhender la réalité singulière du délinquant sexuel dans ses interactions avec le monde qui l’entoure, notre communication vise à montrer l’intérêt d’une approche psychocriminologique qui prenne en compte ces processus de traitement de l’information des auteurs pour comprendre les comportements d’agressions sexuelles et prédire un éventuel risque de récidive. Nous considérons qu’une analyse basée sur des unités médiatrices sociocognitives plus réduites, impliquées dans la construction de schémas ou de scripts comportementaux permet d’apporter un surcroit d’information sur les auteurs. En effet, les unités médiatrices sociocognitives impliquées dans les différents processus d’autorégulation se rapprochent davantage de la signature idiosyncrasique de la personne et affinent la compréhension que nous pouvons avoir des modes comportementaux spécifiques de l’individu dans son interaction avec son environnement. Dans un premier temps, nous avons intégré le concept de schémas selon la classification de YOUNG au modèle du Cognitive-affective personality system (CAPS) développé par MISCHEL & SHODA . Dans un effort d’intégration théorique nous avons comparé cette lecture sociocognitive que nous avons élargie avec différents modèles théoriques en matière d’agression sexuelle. Dans un second temps, nous avons analysé les profils individuels d’auteurs d’infractions à caractères sexuels rencontrés dans un cadre d’expertises et une population témoin. Après avoir analysé les caractéristiques sociodémographiques propres à chaque groupe expérimental, nous avons étudié la prévalence de différentes représentations précoces inadaptées (schémas) ainsi que le caractère dysfonctionnel de ceux-ci parmi les populations étudiées. Au côté de l’approche des schémas, nous avons également évalué les manifestations de personnalités selon les classifications DSM-IV/V. Nous avons comparé les groupes expérimentaux sur ces deux dimensions, des schémas et des caractéristiques de personnalité. Nous avons également observé les éventuels modes préférentiels de schémas et de personnalités selon le type d’infraction à caractère sexuelle. Nous avons évalué les liens existants entre les unités médiatrices sociocognitives que sont les schémas, les troubles de personnalité et les types de comportements sexuels manifestés afin d’évaluer la valeur descriptive et diagnostique de ces différents critères. Enfin, nous avons cherché à voir si la spécificité de ces unités médiatrices, pouvait contribuer à l’évaluation du risque de dangerosité et de récidive par une mise en perspective de nos résultats avec les prédictions du risque obtenues au moyen de la statique-99 de HANSON & THORNTON . [less ▲]

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See detailSpatio-temporal dynamics of β-tubulin isotypes and acetyl-alpha tubulin during the development of the sensory auditory organ in rodents.
Renauld, Justine ULg; Thelen, Nicolas ULg; Johnen, Nicolas ULg et al

Poster (2015, June 05)

The auditory organ is a highly specialized structure composed by specific cellular types. The sensory cells are characterized by stereocilia at their apex and are necessary for the sound perception. These ... [more ▼]

The auditory organ is a highly specialized structure composed by specific cellular types. The sensory cells are characterized by stereocilia at their apex and are necessary for the sound perception. These cells are supported by supporting cells. Supporting cells possess a characteristic cytoskeleton in direct relation with their morphological features and their development. There are different β-tubulin isoforms in microtubules of vertebrate tissues. However, their functional significance is still largely unknown. In the present study, we investigated the localization of five β-tubulin isotypes (β 1 to 5) as well as acetyl-α-tubulin within the hearing organ during development in rodents. By using confocal microscopy, we showed that with the exception of the β3-tubulin isoform that was specific to nerve fibres, all the different β-tubulin isoforms and acetyl-α-tubulin were mainly present in the supporting cells. Contrary to β1-4-tubulins, we also found that the β5-tubulin isoform appeared only at a key stage of the postnatal development in specific cell types (pillar cells and Deiters’ cells). By using transmission electron microscopy, we revealed further that this developmental stage coincided with the formation of two separate bundles of microtubules from a unique one in these supporting cells. In conclusion, these results suggest that the β5-tubulin isoform might be involved in the generation of new microtubule bundles from a pre-existing one. [less ▲]

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See detail"'Only Questions, No Answers': Chris Abani's Dog Woman"
Tunca, Daria ULg

Conference (2015, June 05)

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Peer Reviewed
See detailDe la victimisation à l’agression sexuelle à l’adolescence : dynamiques des Trans-Missions intra et inter-générationnelles
Glowacz, Fabienne ULg

Conference (2015, June 05)

Parmi les facteurs étiologiques du passage à l’acte sexuel, la clinque et de nombreuses études mettent en exergue l’expérience antérieure de victimisation sexuelle chez les auteurs d’infraction à ... [more ▼]

Parmi les facteurs étiologiques du passage à l’acte sexuel, la clinque et de nombreuses études mettent en exergue l’expérience antérieure de victimisation sexuelle chez les auteurs d’infraction à caractère sexuel (AICS). Même si le taux de victimisation sexuelle chez les adolescents AICS est extrêmement variable d’une étude à l’autre, un vécu de victimisation, sans pour autant être le seul facteur étiologique, pourrait être considéré comme un des facteurs de risque dans une constellation plurifactorielle, et ce dans un contexte affectif et social particulier. La méta-analyse de Paolucci, Genuis et Violato (2001) révélait qu’un antécédent d’AS augmentait seulement de 8% le risque de perpétuer le cycle de victime à agresseur. Plutôt que de se centrer sur le débat portant sur les taux de prévalence existant, il nous a semblé intéressant de comprendre comment s’activent les dynamiques transmissives inter et intra-générationnelles. Le concept de cycle intergénérationnel, englobant toute forme de victimisation vécue dans l’enfance du parent et de l’enfant, a surtout été étudié chez l’enfant qui subit un abus sexuel ; notre exposé réactualisera ce concept dans le contexte de l’agression sexuelle commise par l’adolescent. Nous aborderons le cycle et la transmission de l’abus sexuel, d’une part au travers de la victimisation sexuelle d’un parent de l’adolescent commettant une agression sexuelle, et d’autre part, au travers d’un vécu de victimisation sexuelle par l’adolescent qui prend sens au niveau des agressions qu’il va commettre. C’est à partir de trois cas d’adolescent de 14 ans ayant commis des agressions sexuelles sur des enfants de jeune âge (en intra et/ou extrafamilial) que les dynamiques de transmission vont être décodées à partir d’hypothèses psycho-développementales et systémiques. Une première situation se caractérise par la levée du secret de la victimisation de la mère lors du dévoilement de l’agression sexuelle que son adolescent a commise, qui mène à envisager les dynamiques de trans-missions indirectes et de délégation par la mère, s’appuyant sur son incapacité à se connecter et à verbaliser son vécu d’abus et la rage associée, se révélant comme un des médiateurs du processus de transmission. La deuxième situation concerne un adolescent qui, tout comme sa sœur, a subi des abus sexuels par son père, et qui, à son tour, a commis de multiples abus sexuels sur sa sœur. Les dynamiques de transmission par répétition au travers du processus d’identification à l’agresseur et de légitimité destructrice seront développées. Une troisième situation d’un adolescent ayant subi des abus sexuels par un tiers extrafamilial et agissant à son tour à 14 ans des agressions sexuelles sur tous les enfants de la fratrie, nous permettra d’envisager le contexte psycho-affectif familial et de fratrie comme facilitateur de transmission intra-générationnelle. Ces trois situations illustreront la vulnérabilité de l’adolescent qui a été confronté directement ou indirectement dans son histoire personnelle et familiale à un vécu de victimisation, durant cette étape développementale qu’est l’adolescence caractérisée par des enjeux identitaires, relationnels et sexuels. [less ▲]

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