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See detailComparison of xenogeneic graft-versus-host reactions in humanized NSG and NSG-HLA-A2/HHD mice
Ehx, Grégory ULg; SOMJA, Joan ULg; Delens, Loïc ULg et al

Conference (2017, February 01)

Background. So far, the most commonly used mouse model of xenogeneic GVHD is NSG mice injected with human peripheral blood mononuclear cells (PBMCs). However, this model is somewhat limited by the fact ... [more ▼]

Background. So far, the most commonly used mouse model of xenogeneic GVHD is NSG mice injected with human peripheral blood mononuclear cells (PBMCs). However, this model is somewhat limited by the fact that the immunologic reaction of human PBMC is directed exclusively towards murine antigens. Aim. Here, we compared xGVHD induced by injection of human PBMCs in NSG and in NSG mice expressing the HDD construct designed for the expression of human HLA-A0201 convalently bound to human b2 microglobuline, enabling the expression of both HLA-A2 and b2 microglobuline. Methods. NSG and NSG-HLA-A2/HHD (NSG-HLA) mice were transplanted with either HLA-A2+ or HLA-A2− human PBMCs from healthy donors. Survival and xGVHD scores were monitored for the remaining days of the experiments or mice were sacrificed at day 14 to perform various analyzes on their organs. Results. NSG-HLA mice presented overall lower survival rates and higher xGVHD scores than NSG mice (median survival: 28 vs 21 days, p<0.0001). Survival difference between NSG and NSG-HLA mice was higher when they were transplanted with HLA-A2− than with HLA-A2+ donors (HLA-A2−, median survival: 35 vs 25 days, p=0.0001; HLA-A2+, median survival: 23 vs 19 days, p=0.001). Flow cytometry experiments showed higher human CD45+ cells engraftment in NSG-HLA mice in blood, spleen, bone marrow, lungs and liver. Further, lower CD4+/CD8+ T cells ratio were observed in these organs in NSG-HLA mice, highlighting a higher frequency of cytotoxic T cells. These differences of engraftment or ratio could not be attributed to proliferation or activation differences since no variation of either KI67 or HLA-DR were observed between the two mouse models. Supporting the promotion of cytotoxic T cells in NSG-HLA mice, lower frequency of CD45RA+ and higher frequency of TNF-α+ among CD8+ T cells were observed in NSG-HLA in comparison with NSG mice. Finally, preliminary results suggest a specific cytotoxicity toward HLA-A2+ cells in NSG-HLA mice. Conclusion. NSG-HLA-A2/HHD mice develop more severe xGVHD than NSG, characterized by a higher human cell engraftment and higher frequency of cytotoxic T cells, probably reacting specifically toward human HLA-A2 antigen. Therefore these mice may be a better model to study human acute GVHD. [less ▲]

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See detailL'hétérogénéité des individus en terme de longévité et l'influence de celle-ci sur le caractère redistributif des pensions
Denomerenge, Jonathan ULg

Scientific conference (2017, February 01)

Le système belge de pension comporte des éléments de redistribution en faveur des bas revenus. Cependant, l'espérance de vie d'un individu est corrélée positivement avec ses revenus. Par conséquent, les ... [more ▼]

Le système belge de pension comporte des éléments de redistribution en faveur des bas revenus. Cependant, l'espérance de vie d'un individu est corrélée positivement avec ses revenus. Par conséquent, les gens avec un revenu important bénéficient généralement de leur pension de retraite pendant un nombre d'année plus important, ce qui remet potentiellement en cause le caractère redistributif du système. [less ▲]

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See detailIdentification of proteins discriminating inflammation induced dysplasia from simple inflammation in ulcerative colitis by laser capture microdissection and label free proteomics – a pilot study
Merli, Angela-Maria ULg; QUESADA-CALVO, Florence ULg; MASSOT, Charlotte ULg et al

Poster (2017, February 01)

Chronic colonic inflammation in ulcerative colitis (UC) may induce dysplasia, which can itself progress and transform into neoplasia. Diagnosis of dysplasia in UC remains difficult particularly when ... [more ▼]

Chronic colonic inflammation in ulcerative colitis (UC) may induce dysplasia, which can itself progress and transform into neoplasia. Diagnosis of dysplasia in UC remains difficult particularly when tissue inflammation is present. The aim of this retrospective pilot study was to highlight proteins specifically associated with inflammation induced dysplasia in UC. We performed a pilot experiment on 15 Formalin-Fixed, Paraffin-Embedded (FFPE) samples isolated from 5 cases of UC patients with a Polypoïd Pedunculated dysplasia (UC-PP). We compared the proteomes of the UC-PP, the inflammatory (UC-I) and the normal (UC-NL) tissues of each patient. We performed Laser Capture Microdissection (LCM) in order to collect only epithelial cells, avoiding inflammatory infiltrating ones. Label free proteomic analysis using a 2D-nanoUPLC coupled with a hybrid Quadrupole-Orbitrap was applied, as well as differential analysis on the paired samples. Immunohistochemistry (IHC) characterisation of one of the selected proteins of interest was used for validation. Out of 985 quantified proteins, 7 were found significantly more abundant in UC-PP compared to UC-I tissues, with 6 being only detected in UC-PP using proteomics. One of these is Solute Carrier Family 12 member 2 (SLC12A2), also known as Na-K-2Cl co-transporter 1 (NKCC1), a protein involved in ionic balance, in T-cell migration promotion and in some features involved in cancer development like proliferation, migration or invasion. IHC results obtained were in correlation with proteomic results and showed that SLC12A2 was more abundant in UC-PP tissue than in UC-I and UC-NL tissues, with a signal clearly delimiting the dysplastic region from the surrounding inflammatory tissue. This pilot experiment shows a different proteomic profile in inflammation-associated dysplasia and simple inflammation. This should be replicated using other types of dysplasia in IBD. SLC12A2 could be a potential biomarker of inflammation-associated dysplasia. [less ▲]

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See detail“Don't pretend to be my friend!” When an informal brand communication style backfires on social media
Gretry, Anaïs ULg; Horváth, Csilla; Belei, Nina et al

in Journal of Business Research (2017), 74

Social media are now essential platforms for marketing communications, and the volume of consumer-brand in- teractions on these platforms is exploding. Even so, it remains unclear how brands should ... [more ▼]

Social media are now essential platforms for marketing communications, and the volume of consumer-brand in- teractions on these platforms is exploding. Even so, it remains unclear how brands should communicate with consumers to foster relationships and, in particular, to gain their trust. A fundamental decision in this regard is the choice of a communication style, specifically, whether an informal or a formal style should be used in social media communications. In this paper, we investigate how adopting an informal (vs. formal) communication style affects brand trust and demonstrate that using an informal style can either have a positive or negative effect on brand trust, depending on whether consumers are familiar with the brand or not. We further show that these ef- fects occur because consumers expect brands to behave according to social norms, such that the use of an infor- mal style is perceived to be appropriate for familiar brands and inappropriate for unfamiliar ones. [less ▲]

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See detailZinc, manganese and iron interaction within the frd3 Arabidopsis mutant
Scheepers, Maxime ULg; Spielmann, Julien ULg; Goormaghtigh, Erik et al

Poster (2017, February 01)

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See detailImproved Blood Glucose Forecasting Models using Changes in Insulin Sensitivity in Intensive Care Patients
Uyttendaele, Vincent ULg; Dickson, Jennifer; Shaw, Geoff et al

Poster (2017, February 01)

Introduction: Hyperglycaemia, hypoglycaemia and glycaemic variability are associated with worsened outcomes and increased mortality in intensive care units. Glycaemic control (GC) using insulin therapy ... [more ▼]

Introduction: Hyperglycaemia, hypoglycaemia and glycaemic variability are associated with worsened outcomes and increased mortality in intensive care units. Glycaemic control (GC) using insulin therapy has shown improved outcomes, but have been proven difficult to repeat or achieve safely. STAR (Stochastic TARgeted) is a model-based glycaemic control protocol using a stochastic model to forecast distributions of likely future changes in insulin sensitivity (SI) based on its current value. This can be used to determine likely future blood glucose (BG) levels for a given intervention, enabling the most optimal dose selection that best overlaps a clinically defined BG target band. This study presents a novel 3D model capable to predict likely future distribution of SI using both current SI and its prior variability (%ΔSI). Methods: Metabolic data from 3 clinical ICU cohorts totalling 819 episodes and 68629 hours of treatment under STAR and SPRINT protocols are used in this study. Data triplets (%ΔSIn, SIn, SIn+1) are created and binned together in a range of %ΔSI = [-100%, 200%] and SIn = [1.0e-7, 2.1e-3] in bin sizes of %ΔSI = 10% and SIn = 0.5e-4. The 5th, 50th, and 95th percentile of SIn+1 are determined for each bin where data density is high enough (>100 triplets) and compared to the previous stochastic model. The predictive power of the two models are compared by computing median [IQR] per-patient percentage prediction of SI within the 5th-95th and 25th-75th percentile ranges of model predictions. Results: Results show the previous model is over-conservative for ~77% of the data, mainly where %ΔSI is within an absolute 25% change. The percentage change in the 90% CI width in this region is reduced by ~25-40%. Conversely, non-conservative regions are also identified, with 90% CI width increased up to ~80%. Predictive power is similar for both model (60.3% [47.8%, 71.5%] vs. 51.2 [42.9%, 59.2%] within 25th-75th and 93.6% [85.7%, 97.3%] vs. 90.7% [84.4%, 94.6%] within 5th-95th range). Conclusions: The new 3D model achieved similar predictive power as the previous model by reducing the 5th-95th percentile prediction range for 77% of the data, predominantly where SI is stable. If the conservatism of the previous model reduces risk of hypoglycaemia, it also inhibits the controller’s ability to reduce BG to the normal range by safely using more aggressive dosing. The 3D new model thus better characterises patient-specific response to insulin, and allows more optimal dosing, increasing performance and safety. [less ▲]

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See detailMicroalgues: du laboratoire au pilote industriel pour la production de molécules bioactives
Franck, Fabrice ULg; Gerards, Thomas ULg

Scientific conference (2017, February 01)

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See detailStrong XUV irradiation of the Earth-sized exoplanets orbiting the ultracool dwarf TRAPPIST-1
Wheatley, Peter J.; Louden, Tom; Bourrier, Vincent et al

in Monthly Notices of the Royal Astronomical Society (2017), 465

We present an XMM-Newton X-ray observation of TRAPPIST-1, which is an ultracool dwarf star recently discovered to host three transiting and temperate Earth-sized planets. We find the star is a relatively ... [more ▼]

We present an XMM-Newton X-ray observation of TRAPPIST-1, which is an ultracool dwarf star recently discovered to host three transiting and temperate Earth-sized planets. We find the star is a relatively strong and variable coronal X-ray source with an X-ray luminosity similar to that of the quiet Sun, despite its much lower bolometric luminosity. We find L_x/L_bol=2-4x10^-4, with the total XUV emission in the range L_xuv/L_bol=6-9x10^-4. Using a simple energy-limited model we show that the relatively close-in Earth-sized planets, which span the classical habitable zone of the star, are subject to sufficient X-ray and EUV irradiation to significantly alter their primary and perhaps secondary atmospheres. Understanding whether this high-energy irradiation makes the planets more or less habitable is a complex question, but our measured fluxes will be an important input to the necessary models of atmospheric evolution. [less ▲]

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See detailXMM-Newton : en route vers une autre décennie de découvertes
Rauw, Grégor ULg

Article for general public (2017)

Au mois de décembre 2016, cela faisait 17 ans que XMM-Newton fut lancé par une fusée Ariane V. Equipé de ses trois modules miroirs comportant chacun 58 miroirs imbriqués les uns dans les autres, XMM ... [more ▼]

Au mois de décembre 2016, cela faisait 17 ans que XMM-Newton fut lancé par une fusée Ariane V. Equipé de ses trois modules miroirs comportant chacun 58 miroirs imbriqués les uns dans les autres, XMM-Newton est à l’affût de l’émission X de sources cosmiques de tout genre. Malgré sa longévité, XMM-Newton reste à ce jour un des observatoires les plus sollicités au monde. Chaque année, les astrophysiciens proposent des programmes d’observation qui totalisent à peu près 6 fois le temps d’observation disponible! La compétition pour les observations avec XMM est donc très rude. Et a priori cela pourrait continuer encore comme cela pendant quelques années. De fait, les réserves de carburant et la performance des panneaux solaires indiquent que la mission pourrait encore fonctionner au moins 10 ans si les budgets le permettent1! [less ▲]

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See detailGlomerular filtration rate in healthy living potential kidney donors: a meta-analysis supporting the construction of the the Full Age Spectrum equation.
Pottel, H; Hoste, L; Yayo, E et al

in Nephron (2017), 135(2), 105-119

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See detailAttack-Prevention and Damage-Control Investments in Cybersecurity
Lam, Wing Man Wynne ULg

Scientific conference (2017, February)

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See detailLa réforme de l'aide juridique : suivez le guide !
Michiels, Olivier ULg; Falque, Géraldine ULg

in Les droits du justiciable face à la justice pénale (2017)

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See detailIntention, image et singularité selon le premier Sartre : réflexion sur les propriétés et les fonctions de l'acte d'imagination
Recchia, Fabio ULg

in Bulletin d'Analyse Phénoménologique (2017, February)

Partant d’une problématisation de la méthode phénoménologique mise en jeu par "L’Imaginaire", nous proposons de relire les développements de ce texte à partir de la perspective offerte par la théorie de ... [more ▼]

Partant d’une problématisation de la méthode phénoménologique mise en jeu par "L’Imaginaire", nous proposons de relire les développements de ce texte à partir de la perspective offerte par la théorie de l’intentionnalité du premier Sartre. Nous tenterons ainsi de dégager l’unité systématique du projet sartrien d’une « phénoménologie de l’image » en le rapportant aux propositions philosophiques posées de manière décisive par "l’article sur l’intentionnalité". Par ce biais, il s’agira également de fixer les propriétés de la conscience imageante, ainsi que les fonctions jouées par l’acte d’imagination dans l’économie générale de la conscience, en particulier dans l’événement de sa personnalisation. De sorte que nous parcourons les tensions reliant l’imagination de la conscience à son ipséité ; tandis que, du point de vue de l’histoire de la philosophie, nous examinerons certaines des torsions qu’appliqua Sartre sur l’analyse husserlienne de l’image afin d’approfondir celle-ci dans la direction d’une philosophie de la liberté personnelle du sujet. [less ▲]

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See detailClinical settings in knee osteoarthritis: Pathophysiology guides treatment
Herrero-Beaumont, Gabriel; Roman-Blas, Jorge A; Bruyère, Olivier ULg et al

in Maturitas (2017), 96

Osteoarthritis (OA) is the most common chronic joint disorder and its prevalence increases rapidly during midlife. Complex interactions of genetic alterations, sex hormone deficit, and aging with ... [more ▼]

Osteoarthritis (OA) is the most common chronic joint disorder and its prevalence increases rapidly during midlife. Complex interactions of genetic alterations, sex hormone deficit, and aging with mechanical factors and systemic inflammation-associated metabolic syndrome lead to joint damage. Thus, the expression of a clinical phenotype in the early stages of OA relies on the main underlying pathway and predominant joint tissue involved at a given time. Moreover, OA often coexists with other morbidities in the same patient, which in turn condition the OA process. In this scenario, an appropriate identification of clinical phenotypes, especially in the early stages of the disease, may optimize the design of individualized treatments in OA. An ESCEO-EUGMS (European Union Geriatric Medicine Society) working group has recently suggested possible patient profiles in OA. Hereby, we propose the existence of 4 clinical phenotypes – biomechanical, osteoporotic, metabolic and inflammatory – whose characterization would help to properly stratify patients with OA in clinical trials or studies. Further research in this field is warranted. [less ▲]

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