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See detailThe Authority of Strasbourg Jurisprudence from the Perspective of the International Criminal Court
Deprez, Christophe ULg

in Journal Européen des Droits de l'Homme = European Journal of Human Rights (2015), 3

Although the case law of the European Court of Human Rights (Eur. Ct. H.R.) exerts a critical influence on the development of the practice of the International Criminal Court (ICC), it remains unclear ... [more ▼]

Although the case law of the European Court of Human Rights (Eur. Ct. H.R.) exerts a critical influence on the development of the practice of the International Criminal Court (ICC), it remains unclear what the exact authority of such external jurisprudence is vis-à-vis the international criminal judge. This article addresses the question whether ICC judges have used Strasbourg jurisprudence in a consistent manner, in particular as regards the authority which they decided to vest the latter with. It demonstrates that the Court’s chambers have relied on European human rights practice, alternatively, as a mere source of inspiration, as binding interpretation, as a corpus having ambivalent authority, or as a source whose value it simply left unspecified. It therefore concludes that the ICC has been mostly inconsistent regarding the authority of the case law of the Eur. Ct. H.R. in the context of international criminal proceedings. Such inconsistency, it is argued, amounts to a discretionary use of Strasbourg jurisprudence that further reinforces the ICC’s paradoxical position as the ultimate authority for defining the boundaries of its own human rights constraint. [less ▲]

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See detailExtracellular peptidase hunting for improvement of protein production in plant cells and roots
Lallemand, Jérôme ULg; Bouché, Frédéric ULg; Desiron, Carole et al

Poster (2015, June)

Plant-based biopharmaceuticals have gained a lot of interest in the past decade due to their reduced cost and relative safety compared to mammalian cell cultures. While the first plant-made recombinant ... [more ▼]

Plant-based biopharmaceuticals have gained a lot of interest in the past decade due to their reduced cost and relative safety compared to mammalian cell cultures. While the first plant-made recombinant proteins are now reaching the market, the production systems still need improvements to maximize their competitiveness, proteolysis being one of the main factors limiting the yields. Identifying and inhibiting in vivo endogenous proteases involved in the degradation of recombinant proteins could then lead to a significant increase in production yields. In this study, we focused on two different production systems in Arabidopsis thaliana: rhizosecretion and cell suspensions. Extracellular proteases of both systems were used in vitro to study the conditions of target protein degradation (Bovine Serum Albumine, BSA). First, proteases from both systems degrade BSA at both acidic and neutral-to-basic pH conditions. Then, serine and metallopeptidases were shown to be the main protease classes responsible for BSA degradation by rhizosecreted proteomes or extracellular cell culture media, respectively. Finally, the biochemical tests were coupled to a bioinformatics analysis of publicly available transcriptomic data, in order to reduce the number of the proteases most likely involved in BSA degradation. Using this method, only five serine proteases and two metallopeptidases remain candidates for an amiRNA-mediated in vivo inhibition. [less ▲]

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See detailDe novo sequencing using MELD proteolysis coupled to a "sequence assembly" algorithm
Mazzucchelli, Gabriel ULg; Zimmerman, Tyler; Smargiasso, Nicolas ULg et al

in 63rd ASMS Conference Proceedings, May 30 - June 4 2015, St. Louis, MO (2015, June)

Introduction Protein de novo sequencing requires a method that combines extensive MSMS fragmentation and an appropriate data processing. This can be applied either on intact protein or on its proteolytic ... [more ▼]

Introduction Protein de novo sequencing requires a method that combines extensive MSMS fragmentation and an appropriate data processing. This can be applied either on intact protein or on its proteolytic peptides. Peptide analysis has the advantage to be compatible with well-known workflows. Nevertheless the connectivity between peptides is lost. Taking these considerations into account, a specific digestion method and a sequence assembly software were developed. The MELD method relies on a combination of MultiEnzymatic AND Limited proteolytic Digestions. The MELD generates in a single experiment numerous different peptides with miss-cleavages that overlap when aligned on the matching protein sequence. The two major benefices are an increased probability to obtain the entire protein sequence and a redundancy in the protein sequence matches. Methods The MELD consists in two parallel 2h digestions both using an optimized protease mixture. The mixtures are composed of the same proteases but in different relative quantities. Analyses were performed by UPLC-Orbitrap (IClass, Waters, QExactive, Thermo). Data were processed with PEAKS (BSI) to generate de novo sequence candidates. After data importation into our software, a seed sequence is set or can be found automatically. The software extends the seed sequence in both directions with moving windows of three amino acids, plus a fourth one to be added. The added amino acid is validated in several ways, including by the total frequency of occurrence, by larger windows of aa, by the local spectrum-derived confidence, and by combinations of these factors. Preliminary Data The MELD protocol was first validated by applying a traditional database search workflow on several commercial proteins with an inter-day and inter-individual procedure. These experiments showed 100% sequence coverage for each protein analyzed, involving information on peptide identity and modifications localization. Strong confident identification was obtained due to multiple overlapping peptides matches with the given sequence and with a high number of overlapping peptides assignments. The analysis of the four proteins provided the following results: HSA, Myoglobin and Lysozyme were identified with 100% sequence coverage with respectively 890, 300 and 120 unique peptides (CV<10%, peptide FDR<0.1%). The variable region of each heavy and light chains of Adalimumab antibody were identified with 100% sequence coverage. With the MELD, an average of 10 different peptides covering each sequence stretch of the protein could be obtained. The combinatory effect of the multiple enzymes used and the limited digestions leads to an increased robustness, very high confidence identifications and allows clear localization of PTMs. The MELD protocol as presented here and tested on several pure proteins digested in solution, certainly improves the general "bottom-up" strategy applied for highly confident protein identification and would allow better protein characterization, even for those having PTMs. In addition, in our analysis, each fragment position of the entire protein sequence was evidenced either by a "y" or a "b" fragment ion. This high and confident amount of information enables extensive de novo sequencing using PEAKS software, followed by application of our “sequence assembly” algorithm. The first version of our assembling tool on MELD experimental data generated long sequence tags, up to 90 amino acids long. [less ▲]

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See detailPanorama des incitants fiscaux destinés à stimuler l’investissement privé dans le secteur cinématographique
Vandenbulke, Antoine ULg

in Agefi Luxembourg (2015)

The film industry in Europe is largely funded through States support mechanisms. Among the many instruments set up by the various national laws, particular attention should be given to tax incentives ... [more ▼]

The film industry in Europe is largely funded through States support mechanisms. Among the many instruments set up by the various national laws, particular attention should be given to tax incentives aimed to stimulate private capital investments in this sector particularly risky. This short paper aims to outline the main features of tax breaks for the private financing of an audiovisual work in three States in particular: France, Belgium and Luxembourg. [less ▲]

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See detailCROSSROADS le magazine sonore des musiques africaines-américaines
Sacré, Robert ULg

Diverse speeche and writing (2015)

Magazine sonore des musiques africaines-américaines - - - - - page Facebook www.facebook.com/robert.sacre.9 --------- podcast www.mixcloud.com/robertsacre9

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See detailLe financement des entités décentralisées dans la nouvelle Belgique fédérale
Bayenet, Benoît ULg; Husson, Jean-François ULg

in La dotation globale de fonctionnement en question, éléments d'évaluation et perspectives : contributions pour un débat d'actualité (2015)

This publication debates on the current general grant scheme to French municipalities. Our contribution brings a comparison with the Belgian schemes for financing sub-national entities (Regions ... [more ▼]

This publication debates on the current general grant scheme to French municipalities. Our contribution brings a comparison with the Belgian schemes for financing sub-national entities (Regions/Communities and local government). [less ▲]

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See detailNo interest for a second closely-timed infiltration of platelet-rich plasma (PRP) to treat jumper’s knees
Kaux, Jean-François ULg; CROISIER, Jean-Louis ULg; Forthomme, Bénédicte ULg et al

in Radmann, A; Hedenborg, S; Tsolakidis, E (Eds.) 20th annual Congress of the EUROPEAN COLLEGE OF SPORT SCIENCE - BOOK OF ABSTRACTS (2015, June)

Introduction: Some clinical series have evaluated the effect of platelet-rich plasma (PRP) in the treatment of jumper’s knee. Although it is possible that a single infiltrative administration may prove to ... [more ▼]

Introduction: Some clinical series have evaluated the effect of platelet-rich plasma (PRP) in the treatment of jumper’s knee. Although it is possible that a single infiltrative administration may prove to be an effective treatment for this indication, most of the existing studies evaluated the effects of 2 or 3 successive infiltrations. The aim of this study was to evaluate whether 2 infiltrations of PRP proves moreeffective than a single treatment. Methods: Twenty patients suffering from chronic jumper’s knee were enrolled into our prospective, randomized and comparative study of level 2, and split into two randomized groups (1 or 2 infiltrations of PRP, respectively). The 3-month follow-up evaluation consisted of VAS, IKDC and VISA-P scores, along with algometer, isokinetic and ultrasounds evaluations. After 1 year, subjects were contacted to define their functional evolution. Results: The concentration of the PRP used for each infiltration was similar in both groups, and contained no red or white cells. Results revealed no difference in treatment efficacy between the groups.Discussion: The comparison between 1 or 2 infiltrations of PRP did not reveal any difference between the 2 groups at short to mid term. A second closely-timed infiltration of PRP to treat jumper’s knees is not necessary to improve the efficacy of this treatment in the short term. References: Kaux JF, Croisier JL, Bruyere O, Rodriguez de la Cruz C, Forthomme B, Brabant G et al. One injection of platelet-rich plasma associated to a submaximal eccentric protocol to treat chronic jumper’s knee. J Sports Med Phys Fitness. 2014 Jun 19. [Epub ahead of print]. Kaux JF, Croisier JL, Forthomme B, Le Goff C, Buhler F, Savanier C et al. Using platelet-rich plasma to treat jumper’s knees: exploring the effect of a second closely-timed infiltration. J Sci Med Sport. Submitted. Kaux JF, Bruyère O, Croisier JL, Forthomme N, Le Goff C, Rohr N et al. One-year follow-up of platelet-rich plasma infiltration to treat chronic upper patellar tendinopathies. Acta Orthop Belg. In press. [less ▲]

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See detailMélanges en l'honneur de Roger Marijnissen
Verbeeck, Muriel ULg

Book published by CeROArt (2015)

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See detailProceedings of the 12th Workshop on Models and Algorithms for Planning and Scheduling Problems
Marchetti-Spaccamela, Alberto; Crama, Yves ULg; Goossens, Dries et al

Book published by KU Leuven (2015)

This volume contains abstracts of talks presented at the 12th Workshop on Models and Algorithms for Planning and Scheduling Problems (MAPSP 2015), held from June 8 to June 12, 2015, in La Roche-en-Ardenne ... [more ▼]

This volume contains abstracts of talks presented at the 12th Workshop on Models and Algorithms for Planning and Scheduling Problems (MAPSP 2015), held from June 8 to June 12, 2015, in La Roche-en-Ardenne, Belgium. MAPSP is a biennial workshop dedicated to all theoretical and practical aspects of scheduling, planning, and timetabling. The abstracts in this volume include 5 invited talks by Onno Boxma, Michel Goemans, Willem-Jan van Hoeve, Rolf Niedermeier, and Stephan Westphal, plus 88 contributed talks. [less ▲]

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See detailThe ICE protocol for ecological continuity: a new tool to evaluate the upstream fish passage success at physical barriers
Ovidio, Michaël ULg; Larinier, Michel; Burgun, Vincent et al

Conference (2015, June)

The recording and classification of obstacles that can interfere with the movements and migrations of fish is critical information to plan river restoration program. To date a major problem is to ... [more ▼]

The recording and classification of obstacles that can interfere with the movements and migrations of fish is critical information to plan river restoration program. To date a major problem is to determine the potential effect of each obstacle and to select the problematic sites that should be improved to restore longitudinal connectivity (construction of fish passage facilities, removal or modification of the obstacles). Fish passage success about an obstacle is complex and depends on the hydraulic conditions over and at the foot of the obstacle in relation to swimming and leaping capabilities of the fish species concerned. This selection and classification is too often biased because managers lack information’s on the fish capabilities to leap physical obstacles. The need for a simple, reliable and standardised assessment method for use by a wide range of environmental stakeholders rapidly became evident. We responded to the challenge and developed the ICE protocol as the basis for the required ecological-continuity assessment method, particularly in the context of the European Water Framework Directive implementation. The ICE protocol coordinated by ONEMA is based on an integration of the topographical and hydraulic characteristics of obstacles with the physical capabilities (swimming, jumping or crawling) of the fish species analysed. It requires the gathering of standardised descriptive variables on each obstacle. The result of the analysis is an indication on the risks of a structure constituting a more or less severe obstacle (5 classes) for a given fish species (n=47) or group of species. Particular attention was paid to the practical aspects of the method (time required and necessary human resources) to facilitate its use in a wide variety of situations and areas. Each procedure is presented as a flow chart to assist in decision-making, thus making the protocol easy to use for a wide range of people. [less ▲]

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See detailAssessing the passage of obstacles by fish. Concepts, design and application
Baudoin, Jean-Marc; Burgun, Vincent; Chanseau, Matthieu et al

Book published by Onema - Editeur: Véronique Barre (2015)

The fragmentation of habitats has been acknowledged for 30 years as one of the five main factors of biodiversity loss, in conjunction with pollution, overuse of natural resources, invasive species and ... [more ▼]

The fragmentation of habitats has been acknowledged for 30 years as one of the five main factors of biodiversity loss, in conjunction with pollution, overuse of natural resources, invasive species and climate change. Since then, public environmental policies have strived to restore the connectivity of natural habitats. That is particularly the case for water policy, which has made the ecological continuity of rivers a central element in policy planning, a quality element for monitoring programmes and one of the basic guidelines for river-basin management plans (RBMP). The time has thus come for decisions, with the resulting controversy given that any attempt to modify existing discontinuities impacts our perception of landscapes and various uses of aquatic environments. Given that there is, on average, at least one obstacle for every five kilometres of river in continental France, this issue concerns the entire country, its population and all water managers. That explains why it was necessary to have a single set of standardised procedures for selecting the work to be done on the basis of objective and comparable data. For the ecological continuity of fish, we now have the ICE method presented in this book, which describes obstacles in rivers and assesses the capacity of fish to overcome those obstacles during their upstream migration. The design, development and national deployment of this method required five years of intense, collective effort on the part of numerous scientists and the Onema local and regional offices. The method is the result of an outstanding multi-disciplinary approach involving both hydraulics and ecology, two disciplines that some people might see as irreconcilable, but that must work together synergistically in the effort to restore environments. The publication of this book in the Knowledge for action series marks the transition from the team that developed the method to the people who will use it to acquire better understanding of ecological continuity in rivers and as an operational tool in implementing water policy and enhancing biodiversity. [less ▲]

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See detailOn n'est pas à une seconde près, si?
Van Der Beken, Emeline ULg

Article for general public (2015)

L'année 2015 comptera une seconde supplémentaire. Le passage du 30 juin 2015 au 1 juillet 2015 suivra la séquence temporelle suivante: 23 h 59 min 59 s, 23 h 59 min 60 s, 0 h 0 min 0 s. L'ajout de cette ... [more ▼]

L'année 2015 comptera une seconde supplémentaire. Le passage du 30 juin 2015 au 1 juillet 2015 suivra la séquence temporelle suivante: 23 h 59 min 59 s, 23 h 59 min 60 s, 0 h 0 min 0 s. L'ajout de cette seconde, dite seconde intercalaire, a pour objectif de conserver le temps légal en synchronisation avec le mouvement de rotation de la Terre sur elle-même. [less ▲]

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See detailEGFR and NF-kB: partners in cancer
Shostak, Kateryna ULg; Chariot, Alain ULg

in Trends in Molecular Medicine (2015), 21

Oncogenic proteins cooperate to promote tumor development and progression by sustaining cell proliferation, survival and invasiveness. Constitutive EGFR and NF-kappaB activities are seen in multiple solid ... [more ▼]

Oncogenic proteins cooperate to promote tumor development and progression by sustaining cell proliferation, survival and invasiveness. Constitutive EGFR and NF-kappaB activities are seen in multiple solid tumors and combine to provide oncogenic signals to cancer cells. Understanding how these oncogenic pathways are connected is critical, given their role in intrinsic or acquired resistance to targeted anti-cancer therapies. We review molecular mechanisms by which both EGFR- and NF-kappaB-dependent pathways establish positive loops to increase their oncogenic potential. We also describe how NF-kappaB promotes resistance to EGFR inhibitors. [less ▲]

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See detailSmall-Angle Scattering in Porous Solids, an overview highlighting data analysis challenges
Gommes, Cédric ULg

Conference (2015, June)

Countless technologies and chemical processes make use of nanoporous materials: heterogeneous catalysis, including electrochemical reactions in fuel-cell electrodes, adsorption separation processes ... [more ▼]

Countless technologies and chemical processes make use of nanoporous materials: heterogeneous catalysis, including electrochemical reactions in fuel-cell electrodes, adsorption separation processes, kinetically selective membrane processes, are but a few examples. Nanopores are also relevant to natural processes as diverse as the weathering of rocks and ion transport through biological membranes.[1] Small-angle scattering of x-rays (SAXS) or neutrons (SANS) is one of the few experimental methods currently available for the in situ analysis of phenomena in this type of materials at the mesoscopic scale.[e.g. 2,3] In this presentation, we briefly review some recent applications of small-angle scattering to the in situ analysis of phenomena inside mesoporous solids. A particular focus is put on the data analysis challenges, whereby the scattered intensity is converted to real-space structures with nanometer resolution. [less ▲]

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See detailQuaternary prevention: first, do not harm - Editorial
Jamoulle, Marc ULg

in Revista Brasileira de Medicina de Família e Comunidade (2015), 10(35), 1-3

Clinical prevention, under the influence of public health, has been organised in a chronological manner since the middle of the 20th century. A paradigm shift from a chronological to a constructivist ... [more ▼]

Clinical prevention, under the influence of public health, has been organised in a chronological manner since the middle of the 20th century. A paradigm shift from a chronological to a constructivist relationship-based preventive pattern of care offers new insights into the practice of doctors. This paradigm shift brings to light the concept of quaternary prevention, a critical look at medical activities with an emphasis on the need not to harm. Quaternary prevention addresses the fundamental question of what constitutes too much or too little medicine. It is the fourth form of disease prevention, but also the fourth frame of action for family doctors [less ▲]

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See detailA comparative study of triaxial and uniaxial magnetic shields made out of YBCO coated conductors
Wera, Laurent ULg; Fagnard, Jean-François ULg; Levin, George et al

in Superconductor Science and Technology (2015), 28

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See detailPrevenção quaternária: primeiro não causar dano - Editorial
Jamoulle, Marc ULg

in Revista Brasileira de Medicina de Família e Comunidade (2015), 10(35), 1-3

A prevenção clínica, sob a influência da saúde pública, foi organizada de forma cronológica, desde meados do século XX. No entanto, uma mudança de paradigma de uma ordem cronológica para um padrão ... [more ▼]

A prevenção clínica, sob a influência da saúde pública, foi organizada de forma cronológica, desde meados do século XX. No entanto, uma mudança de paradigma de uma ordem cronológica para um padrão construtivista fundamentado em uma relação preventiva de atendimento, oferece novas,reflexões à prática dos médicos. Essa mudança de paradigma traz à tona o conceito de prevenção quaternária, um olhar crítico sobre as atividades médicas, com ênfase na necessidade de não causar danos. A prevenção quaternária aborda a questão principal do que se constitui como ‘muita’ ou ‘pouca’ medicina. Ela é a quarta forma de prevenção de doenças, e também a quarta etapa de ação dos médicos de família [less ▲]

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See detailThe strength of multi-row models
Louveaux, Quentin ULg; Poirrier, Laurent; Salvagnin, Domenico

in Mathematical Programming Computation (2015), 7(2), 113-148

We develop a method for computing facet-defining valid inequalities for any mixed-integer set PJ. Our practical implementation does not return only facet- defining inequalities, but it is able to find a ... [more ▼]

We develop a method for computing facet-defining valid inequalities for any mixed-integer set PJ. Our practical implementation does not return only facet- defining inequalities, but it is able to find a separating cut whenever one exists. The separator is not comparable in speed with the specific cutting-plane generators used in branch-and-cut solvers, but it is general-purpose. We can thus use it to compute cuts derived from any reasonably small relaxation PJ of a general mixed- integer problem, even when there exists no specific implementation for computing cuts with PJ. Exploiting this, we evaluate, from a computational perspective, the usefulness of cuts derived from several types of multi-row relaxations. In particular, we present results with four different strengthenings of the two-row intersection cut model, and multi-row models with up to fifteen rows. We conclude that only fully-strengthened two-row cuts seem to offer a significant advantage over two-row intersection cuts. Our results also indicate that the improvement obtained by going from models with very few rows to models with up to fifteen rows may not be worth the increased computing cost. [less ▲]

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