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See detailOpinions, attitudes et comportements politiques. Tome II
Grandjean, Geoffrey ULg

Learning material (2016)

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See detail10 years of advances in nonlinear system identification in structural dynamics: A review
Noël, Jean-Philippe ULg; Kerschen, Gaëtan ULg

in Proceedings of ISMA 2016 - International Conference on Noise and Vibration Engineering (2016, September)

Nonlinear system identification is a vast research field, today attracting a great deal of attention in the structural dynamics community. Ten years ago, an MSSP paper reviewing the progress achieved ... [more ▼]

Nonlinear system identification is a vast research field, today attracting a great deal of attention in the structural dynamics community. Ten years ago, an MSSP paper reviewing the progress achieved until then concluded that the identification of simple continuous structures with localised nonlinearities was within reach. The past decade witnessed a shift in emphasis, accommodating the growing industrial need for a first generation of tools capable of addressing complex nonlinearities in larger-scale structures. The objective of the present paper is to survey the key developments which arose in the field since 2006 towards developing these tools. [less ▲]

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See detailDiurnal variations of hormonal secretion, alertness and cognition in extreme chronotypes under different lighting conditions.
Maierova, L.; Borisuit, A.; Scartezzini, L et al

in Scientific Reports (2016)

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See detailOpinions, attitudes et comportements politiques. Tome III
Grandjean, Geoffrey ULg

Learning material (2016)

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See detailLes usages des manuels dans les préparations des enseignants stagiaires
Scheepers, Caroline ULg

in Français Aujourd'Hui (Le) (2016), 194

La présente contribution se propose d’analyser la façon dont les manuels sont convoqués par les stagiaires dans leurs préparations de cours de français. Dès lors, la focalisation se place non sur les ... [more ▼]

La présente contribution se propose d’analyser la façon dont les manuels sont convoqués par les stagiaires dans leurs préparations de cours de français. Dès lors, la focalisation se place non sur les manuels en tant quel tels, mais sur leur réception et leur réinvestissement dans les pratiques des étudiants. Ont été analysées une centaine de fiches de préparation élaborées par de futurs enseignants belges, qu’ils se destinent à enseigner au primaire, au secondaire inférieur (collège) ou supérieur (lycée). Les usages du manuel révèlent et construisent des différences notables en terme de compétences enseignées, de gestes pédagogiques mobilisés, de rapport au savoir, de culture professionnelle, de formation… Étudier la réception et l’utilisation du manuel revient à problématiser des pratiques et des représentations fondamentales qui touchent à ce que veulent dire enseigner, (faire) apprendre ou construire une leçon. Cette recherche éclaire une facette du processus de planification des apprentissages. [less ▲]

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See detailGait pattern of healthy old people for dual task walking condition
GILLAIN, Sophie ULg; Boutaayamou, Mohamed ULg; Schwartz, Cédric ULg et al

in Gerontechnology (2016, September)

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See detailGenetics of pituitary adenomas
Rostomyan, Liliya ULg; Beckers, Albert ULg

Scientific conference (2016, September)

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See detailLe Cifen actuel en cinq points
Simons, Germain ULg

Article for general public (2016)

Dans le cadre de la table ronde sur les 20 ans du Cifen, G. Simons, président actuel du Centre interfacultaire de formation des enseignants (Cifen) de l'ULg, a présenté le centre en 5 grands points.

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See detailGait pattern of healthy old people for fast walking condition
GILLAIN, Sophie ULg; Boutaayamou, Mohamed ULg; Schwartz, Cédric ULg et al

in Gerontechnology (2016, September)

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See detailL'EBM peut-elle encore enthousiasmer ?
Henrard, Gilles ULg

in Minerva: Tijdschrift Voor Evidence Based Medicine (2016)

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See detailValidation of Prediction Models for Near Adult Height in Children with Idiopathic Growth Hormone Deficiency Treated with Growth Hormone A Belgian Registry Study
Straetemans, Saartje; De Schepper, Jean; THOMAS, Muriel et al

in Hormone Research in Paediatrics (2016), 86

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See detailGalileo Cycle-Slip Detection. How Four Frequencies Help When the Ionosphere Is Disturbed.
Van de Vyvere, Laura; Warnant, René ULg

in GPS World (2016), 27(9), 50-56

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See detailProduction of word stress by French speaking CLIL and non-CLIL learners of Dutch
Hiligsmann, Philippe; Rasier, Laurent ULg; Degrave, Pauline

Conference (2016, September)

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See detailIdentification of bovine and porcine colistin-resistant mcr1-positive Escherichia coli.
Mainil, Jacques ULg; Muylaert, Adeline ULg; Saulmont, Marc et al

Conference (2016, September)

OBJECTIVE Polymyxins, especially colistin, have been used for years in veterinary medicine and were rediscovered a few years ago as last resort antibiotics in human medicine against multi-resistant Gram ... [more ▼]

OBJECTIVE Polymyxins, especially colistin, have been used for years in veterinary medicine and were rediscovered a few years ago as last resort antibiotics in human medicine against multi-resistant Gram negative bacterial pathogens. For years, only chromosome-mediated resistance to colistin was identified as a consequence of mutation(s) in lipid A-encoding genes. Recently, however, a plasmid-located gene (mcr1) was identified in Gram-negative enterobacteria and has since been found by PCR in several, but not all, bovine, human, porcine and poultry colistin-resistant Escherichia coli (Liu YY et al. Lancet Infect Dis, 2016, 16(2), 161-168; Nordmann P and Poirel L. Clin Microbiol Infect, 2016, 22, 398-400 ; Schwarz S and Johnson AP. J Antimicrob Chemother, 2016, in press, doi: 10.1093/jac/dkw274). The purpose of this study was to compare phenotypic and genetic for the detection of resistance to colistin and of the mcr1 gene in a collection of Escherichia coli isolated from different animal species and from humans. METHODS More than 3000 E. coli isolates from cattle, pigs, dogs, cats, horses, rabbits, chickens ducks and humans were tested for resistance to colistin by growing them on agar plates with 1g/ml of colistin. The Minimal Inhibitory Concentrations (MIC) of and the presence of the mcr1 gene in all growing isolates were determined using the E test® and colony hybridization assay with a mcr1 specific gene probe, respectively. The probe-positive isolates were further tested with the mcr1 gene specific PCR. RESULTS A total of 410 E. coli isolated grew on 1g/ml colistin-containing agar plates. The majority of isolates grew well, but several grew sparsely with only few isolated colonies. As determined by the E test®, MIC of 273 isolates (67%) was 1g/ml of colistin and higher; conversely, MIC of 137 isolates (33%) was lower than 1g/ml of colistin. Of those 410 E. coli isolates, 34 from pigs and bovines (9% of isolates growing on colistin-containing agar plates; 25% of isolates with MIC higher than 1g/ml) hybridized with the mcr1 gene-derived probe: 5 from pigs and 11 from bovines gave black spots (including five from the same calf), while 18 from pigs and one from bovine gave grey spots. All but one pig isolate had a MIC between 1.5 and 16 g/ml of colistin. Fifteen “black spot” probe-positive isolates tested positive with the mcr1 gene specific PCR as did 3 porcine “grey spot” probe-positive isolates, while the remaining 16 isolates repeatedly tested negative even after lowering the annealing temperature. CONCLUSION This study confirms that (i) the results of phenotypic assays for the detection of colistin resistance can not be always trusted; (ii) the mcr1 gene is not the only one mechanism of resistance to colistin; (iii) mcr1 variants may exist that can not be detected by the classical PCR. Phenotypic assays like growth on colistin-containing agar plates can still represent a first base screening assay, although the MIC determination using the E test® confirms a >1g/ml MIC for only 2 out of 3 growing isolates. Presence of mcr1 gene and putative variants (like the most recently described mcr2 gene; Xavier BB et al., Eurosurveillance, 21, 7 July 2016) in all probe-positive isolates will be confirmed after Whole Genome Sequencing that will also allow comparing the mcr1-positive plasmids and isolates from pigs and cattle to similar human E. coli isolates. Further studies should also be performed to identify the colistin resistance mechanism in mec-negative isolates. [less ▲]

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See detailQuelles perspectives d'avenir pour le Cifen ?
Simons, Germain ULg

Article for general public (2016)

Dans le cadre de la table ronde portant sur les 20 ans du Cifen (28 août 2015), le président actuel du Centre, G. Simons, a évoqué quelques perspectives pour le centre.

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See detailNonlinear vibration analysis of the SmallSat spacecraft: From identification to design
Detroux, Thibaut ULg; Noël, Jean-Philippe ULg; Masset, Luc ULg et al

Conference (2016, September)

The objective of the present contribution is to introduce nonlinear experimental and numerical tools and methods applicable to real-life structures. The study is illustrated using the SmallSat spacecraft ... [more ▼]

The objective of the present contribution is to introduce nonlinear experimental and numerical tools and methods applicable to real-life structures. The study is illustrated using the SmallSat spacecraft developed by Airbus Defence and Space, which possesses several localized nonlinearities. The computation of nonlinear normal modes and bifurcations reveals that the satellite possesses complex dynamics including modal interactions, quasiperiodic oscillations and isolated resonances. [less ▲]

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See detailIsolation of bacteriophages against Klebsiella pneumoniae and in vivo activity
Thiry, Damien ULg; Passet, Virginie; Dufour, Nicolas et al

Poster (2016, September)

Klebsiella pneumoniae is a bacterial pathogen able to induce severe healthcare-associated or community-acquired infections in humans and animals. The constant emergence of antibiotic resistant strains ... [more ▼]

Klebsiella pneumoniae is a bacterial pathogen able to induce severe healthcare-associated or community-acquired infections in humans and animals. The constant emergence of antibiotic resistant strains reinforces the need to find alternatives to antibiotic treatments. The use of bacteriophages is a promising approach. The aim of this study was to isolate bacteriophages directed against K. pneumoniae strains and to test their efficacy in a murine model. Bacteriophages against five different K. pneumoniae (2 of capsular type K1 and K2 and 1 undetermined) were isolated and purified from waste water collected in Paris area. The morphology of plaques (zones of bacterial killing) was recorded and several of them were purified three times by successive replating. Phage titers were determined by serial dilutions on their respective hosts as well as on 18 other Klebsiella strains to identify their host range. Kinetics of bacterial lysis were monitored during 15h at 3 multiplicities of infection, in triplicates. For in vivo experiment, a total of 10 mice were inoculated with 200 µl of K. pneumoniae (4.6E+07 CFU) by oral gavage and the level of K. pneumoniae in fecal samples was monitored for 10 days. Five mice did not receive any treatment and 5 other mice received a cocktail of three bacteriophages (8E+07 PFU) at day 4 post-inoculation. A total of 54 bacteriophages were isolated and purified with titers ranging from 2E+5 to 3.6E+10 PFU/ml. The host range study showed that bacteriophages against K. pneumoniae have a specificity related to the capsular type of their bacterial host. Lysis kinetics of bacteria suggested that different phages were isolated. Despite difficulties with the murine intestinal model, evidence was obtained that bacteriophages are able to reduce intestinal carriage. Our results show that bacteriophages isolated against K. pneumoniae are specific for a given capsular type, although further studies are necessary to provide more details on this capsular specificity and its molecular determinants. To fully address the in vivo potential of phages, a reliable mouse model of intestinal carriage of K. pneumoniae strains needs to be established. [less ▲]

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See detailIdentification of bovine methicillin resistant staphylococci from Europe, Africa and North America by colony hybridization, PCR and antibiotic sensitivity.
Ngassam Tchamba, Cyrille ULg; Thiry, Damien ULg; Bardiau, Marjorie et al

Conference (2016, September)

Mastitis is the costliest pathology in dairy cattle and staphylococci are the most prevalent bacterial mastitis pathogens worldwide. Antimicrobial treatment of mastitis has led to the selection of ... [more ▼]

Mastitis is the costliest pathology in dairy cattle and staphylococci are the most prevalent bacterial mastitis pathogens worldwide. Antimicrobial treatment of mastitis has led to the selection of resistant staphylococci, of which the Methicillin Resistant S. aureus (MRSA) are the most studied ones. Still, MR has also been described for non-aureus staphylococci (MRS) species. Bovine MRS(A) represent not only a problem in the treatment of mastitis, but also a potential hazard in public health via the inter-Staphylococcus transferability of the mobile “Staphylococcal Cassette Chromosome” (SCC) carrying the mec genes encoding MR and the zoonotic potential of some Staphylococcus species. The aim of this study is the comparison of genetic and phenotypic methods for the identification of MRS(A) isolated from bovine mastitis in European, African and North-American countries. A total of 1168 mastitis-associated staphylococci were isolated between 2005 and 2014 in Belgium, Italy, Switzerland, Senegal, Niger and Canada, and kept at -80°C until further use. Out of them, 867 isolates were identified to S. aureus while 301 isolates were non aureus staphylococci. All 1168 staphylococci were tested genetically by the dot blot hybridization assay on positively charged nylon membranes (Roche) after DNA extraction with 32P-radioactively labelled probes derived from the mecA and mecC genes and phenotypically by growth on “Chrom MRSA ID®” agar plates. Isolates positive at both or either tests were further studied by PCR targeting the same two genes and by the disk diffusion assay to oxacillin and cefoxitin. A total of 265 isolates (23%) were positive at both or either tests. Out of them, 27 S. aureus (10%) but no non-aureus (0%) tested positive both for DNA hybridization with the mecA probe and for growth on “Chrom MRSA ID®” plates. No isolate tested positive with the mecC probe. In addition, 32 S. aureus (12%) and 15 non aureus (6%) were positive with the mecA probe only and 169 S. aureus (64%) and 22 non aureus (8%) grew on “Chrom MRSA ID®” plates only. The S. aureus originate from Belgium (105), Italy (6), Canada (31), Senegal (38) and Niger (48) whereas the non-aureus originate from Belgium (25), Italy (1) and Niger (11). All of them are being tested with the PCR targeting the mecA gene and by the disk diffusion assay to oxacillin and cefoxitin. Most isolates (72%) grew on “Chrom MRSA ID®” plates only while few (18%) were positive to the hybridization with the mecA probe only. This high difference between the results of both tests could be explained by the weak specificity of phenotypic tests comparing to genetic tests. The others 10% of the isolates (S. aureus) which are positive with the two methods (dot blot hybridization and “Chrom MRSA ID®”) can be considered as MRSA mediated by the mecA gene. However, results of PCR and disk diffusion assay will confirm respectively the presence of mec genes and which of the two methods is the most suitable for identifying MRS from mastitis cases in cattle. Comparison of the results of phenotypic and genetic assays will indicate whether other variant(s) than mecA and mecC may be present in MRS. Further genetic and phenotypic studies are needed to (i) identify the non-aureus isolates to the species level; (ii) compare the MRS(A) isolated in the different countries by their biotypes, serotypes, lysotypes, and virulotypes, without forgetting their SCCmec and their clonal complex; and (iii) identify the mec gene variant present in hybridization-positive PCR-negative isolates, if any. [less ▲]

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See detailDu processus aux soins intégrés: Expérience de gestion de projet bottom-up
ERPICUM, Marie ULg; BASSLEER, Bernard ULg; DEFRAIGNE, Jean ULg et al

in Gestions Hospitalieres (2016), 558(Août/septembre 2016),

Le service de chirurgie cardiaque du CHU de Liège a soutenu des recherches visant à développer un programme d’épargne sanguine, enjeu actuel de taille dans ce secteur des soins de santé. Ce projet a ... [more ▼]

Le service de chirurgie cardiaque du CHU de Liège a soutenu des recherches visant à développer un programme d’épargne sanguine, enjeu actuel de taille dans ce secteur des soins de santé. Ce projet a évolué vers la création d’un itinéraire clinique chirurgical cardiaque et d’un modèle institutionnel pour le développement d’autres itinéraires cliniques. Une évolution qui permet de déterminer les missions spécifiques de l’institution et ses objectifs stratégiques, et de s’associer aux projets nationaux. L’adhésion multidisciplinaire, soutenue par un leadership médical et infirmier, ainsi que la reconnaissance institutionnelle sont les déterminants de la pérennité de cette démarche bottom-up. [less ▲]

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