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See detailAIRE polymorphism, melanoma antigen-specific T cell immunity, and susceptibility to melanoma
Conteduca, Giuseppina ULg

in Oncotarget (2016)

AIRE is involved in susceptibility to melanoma perhaps regulating T cell immunity against melanoma antigens (MA). To address this issue, AIRE and MAGEB2 expressions were measured by real time PCR in ... [more ▼]

AIRE is involved in susceptibility to melanoma perhaps regulating T cell immunity against melanoma antigens (MA). To address this issue, AIRE and MAGEB2 expressions were measured by real time PCR in medullary thymic epithelial cells (mTECs) from two strains of C57BL/6 mice bearing either T or C allelic variant of the rs1800522 AIRE SNP. Moreover, the extent of apoptosis induced by mTECs in MAGEB2-specific T cells and the susceptibility to in vivo melanoma B16F10 cell challenge were compared in the two mouse strains. The C allelic variant, protective in humans against melanoma, induced lower AIRE and MAGEB2 expression in C57BL/6 mouse mTECs than the T allele. Moreover, mTECs expressing the C allelic variant induced lower extent of apoptosis in MAGEB2-specific syngeneic T cells than mTECs bearing the T allelic variant (p < 0.05). Vaccination against MAGEB2 induced higher frequency of MAGEB2-specific CTL and exerted higher protective effect against melanoma development in mice bearing the CC AIRE genotype than in those bearing the TT one (p < 0.05). These findings show that allelic variants of one AIRE SNP may differentially shape the MA-specific T cell repertoire potentially influencing susceptibility to melanoma. [less ▲]

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See detailNew role of osteopontin in DNA repair and impact on human glioblastoma radiosensitivity
Henry, Aurélie ULg; Nokin, Marie-Julie ULg; Leroi, Natacha ULg et al

in Oncotarget (2016)

Glioblastoma (GBM) represents the most aggressive and common solid human brain tumor. We have recently demonstrated the importance of osteopontin (OPN) in the acquisition/maintenance of stemness ... [more ▼]

Glioblastoma (GBM) represents the most aggressive and common solid human brain tumor. We have recently demonstrated the importance of osteopontin (OPN) in the acquisition/maintenance of stemness characters and tumorigenicity of glioma initiating cells. Consultation of publicly available TCGA database indicated that high OPN expression correlated with poor survival in GBM patients. In this study, we explored the role of OPN in GBM radioresistance using an OPN-depletion strategy in U87-MG, U87-MG vIII and U251-MG human GBM cell lines. Clonogenic experiments showed that OPN-depleted GBM cells were sensitized to irradiation. In comet assays, these cells displayed higher amounts of unrepaired DNA fragments post-irradiation when compared to control. We next evaluated the phosphorylation of key markers of DNA double-strand break repair pathway. Activating phosphorylation of H2AX, ATM and 53BP1 was signi cantly decreased in OPN-de cient cells. The addition of recombinant OPN prior to irradiation rescued phospho-H2AX foci formation thus establishing a new link between DNA repair and OPN expression in GBM cells. Finally, OPN knockdown improved mice survival and induced a signi cant reduction of heterotopic human GBM xenograft when combined with radiotherapy. This study reveals a new function of OPN in DNA damage repair process post-irradiation thus further con rming its major role in GBM aggressive disease. [less ▲]

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See detailANALYSE DE LA DEMANDE EN CHARBON DE BOIS PAR LES MÉNAGES URBAINS DE BUJUMBURA AU BURUNDI
Sabuhungu, Emery Gaspard ULg

Doctoral thesis (2016)

The objective of this study is to evaluate the demand for charcoal by urban households in Bujumbura in order to make recommendations ensuring the security of household energy and environmental protection ... [more ▼]

The objective of this study is to evaluate the demand for charcoal by urban households in Bujumbura in order to make recommendations ensuring the security of household energy and environmental protection. To achieve this objective, the analysis of the related documentation and collection of secondary data were carried out in order to formulate specific objectives, hypotheses and the methodology used in the course of this study. The specific objectives are: to analyze the structure, operation and market performance of the charcoal market in the city of Bujumbura and to compare this market to the cities of Lubumbashi and Kigali; to determine the charcoal consumption pattern by urban households in Bujumbura; to identify the factors which influence the demand for charcoal by urban households in Bujumbura; to evaluate the demand for charcoal by urban households in Bujumbura. The assumptions made in this study are: the charcoal market in the city of Bujumbura is a competitive and effective market; charcoal is the dominating cooking fuel used by urban households in Bujumbura and is consumed exclusively or in combination with others; an urban household’s demand for charcoal in Bujumbura is essentially based on household income, the size of household, eating habits (preparation of food that requires a long cooking time), the frequency of use of carbon and the possession or not of improved cooking stoves; the demand for charcoal by urban households in Bujumbura is high and this leads to significant annual deforestation. Three surveys were conducted to collect primary data. The first survey, with the specific objective of analysing the charcoal market in Bujumbura, was conducted among the key players of the charcoal value chain produced in the communes of Mugamba and Muramvya and consumed in the city of Bujumbura. The remaining two surveys, with the specific objective of analysing the consumption of charcoal by households, were conducted among households-users of charcoal of different standards of living in three areas of the city of Bujumbura (areas Buterere, Cibitoke and Rohero). The results indicate that the charcoal market is competitive. The production, the commercialisation and the consumption of charcoal allow the creation of positive value added all along the charcoal value chain. The use of cooking fuels by households in Bujumbura shows that they have not yet achieved an energy transition. In fact, 83% of those surveyed only use charcoal; 12% combine charcoal and electricity and 5% combine charcoal and firewood. The results revealed that the main factors influencing households demand for charcoal are: household income, the price of charcoal, household size, number of cooking sessions per day, frequency of preparation of cassava leaves and the possession or not of improved braziers. To solve the energy and environmental problem, a rehabilitation of woodland, an implementation of the best techniques of woodland planting with the most adaptable species on productive land, a greater penetration of substitutable energy to wood fuel such as gas or electricity, a growth in the use of improved cooking stoves in households and greater efficiency in wood carbonisation will help reduce the number of hectares of deforestation for wood energy needs. [less ▲]

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See detailDesign of microstructures using stress-based topology optimization
Collet, Maxime ULg; Bruggi, Matteo; Noël, Lise ULg et al

Conference (2016, August 22)

This paper aims at designing microstructures using stress-based topology optimization. Most of the developments so far have been made for compliance design in various field of applications as reflected in ... [more ▼]

This paper aims at designing microstructures using stress-based topology optimization. Most of the developments so far have been made for compliance design in various field of applications as reflected in the literature. The emergence of the new additive manufacturing techniques allows to consider porous material, such as lattice structures for instance, which ca be used for the design of structural components subject to various solicitations. Those components must account for the stress level to prevent failure everywhere in the microstructures and by extension the whole structure itself. This work proposes to design such microstructures using topology optimization with limitation on the stress level within the microstructures before printing the result. The homogenization technique is used to determine the equivalent material properties. The issues and perspectives are also discussed. [less ▲]

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See detailAu-delà des querelles d'écoles, quels fondements pour un enseignement efficace et de qualité ?
Lafontaine, Dominique ULg

Conference given outside the academic context (2016)

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See detailIs the observer effect significant in vegetation assessment of restored metallicolous grassland?
Boisson, Sylvain ULg; Mahy, Grégory ULg

Conference (2016, August 22)

Projects that prospects habitat restoration require protocols of vegetation assessment to monitor the evolution of the restored ecosystems over time. In most cases, multiple assessors are needed and some ... [more ▼]

Projects that prospects habitat restoration require protocols of vegetation assessment to monitor the evolution of the restored ecosystems over time. In most cases, multiple assessors are needed and some studies have shown that the variability among observer can be significant. In the context of tropical grassland, few studies quantified the impact of the observer on the results, reducing the capacity to replicate conclusions, and, therefore to plan restoration strategies at large scale. This study aims to quantify the variation among observers using two phytosociological methods on restored metallicolous grassland and to test whether variation in the assessment could involve the conclusions of the vegetation assessment. Four observers were selected to perform monitoring of quadrats in two distinct habitats identified in four restored ecosystems. All observers sampled seven metrics based on phytosociological methods and two of them also performed a complete vegetation sampling of the quadrat, including species identification and cover in quadrat. For metrics based on cover, variation of measure exists among the observers. For metrics based on counting, no variation is highlighted and the observers are able to distinguish and identify species. These results emphasize that a same method could lead to distinct results according to observer and, in restoration projects, it is crucial to ensure a correct transfer of protocols of vegetation assessment in order to reproduce equally the method. [less ▲]

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See detailOne pot synthesis by plasma treatment of Pt-based catalysts for PEM fuel cells
Busby, Yan; Gulas, Michal; Laurent-Brocq, Mathilde et al

Conference (2016, August 22)

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See detailAbility of Tuta absoluta (Lepidoptera: Gelechiidae) to develop on alternative host plant species
Bawin, Thomas ULg; Dujeu, David; De Backer, Lara ULg et al

in Canadian Entomologist (2016), 148(4), 434-442

The tomato leafminer, Tuta absoluta Meyrick (Lepidoptera: Gelechiidae), is a widespread devastating pest reported to develop on economically important solanaceous crops. The characterization of its host ... [more ▼]

The tomato leafminer, Tuta absoluta Meyrick (Lepidoptera: Gelechiidae), is a widespread devastating pest reported to develop on economically important solanaceous crops. The characterization of its host range could help to understand and prevent the dispersion behavior of the insect in the environment. In this study, the ability of T. absoluta to develop on 12 cultivated or non-cultivated plants including Solanaceae, Chenopodiaceae, Convolvulaceae, Fabaceae and Malvaceae species under laboratory conditions was assessed. For each plant species, we monitored the development times of immature stages, survival, sex ratios and adult fecundity rates. All the 6 tested non-solanaceous plants, including Chenopodium, Convolvulus and Malva species, were not able to sustain (i.e. allow growth and development) T. absoluta larvae. Among Solanaceae, Solanum species were the most suitable host plants for the pest, but others could be opportunistically colonized with fewer incidences. T. absoluta appears to be strongly related to solanaceous plants which would predominantly support self-sustaining field populations. Preventing crop infestation by removing potential host plants in the immediate field vicinity and culture rotations with non-solanaceous crops is of primary importance. [less ▲]

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See detailBicycle tour in the city and site visit
Frankignoulle, Pierre ULg

Diverse speeche and writing (2016)

Visite de ville et des lieux que les participants à la Summer school vont avoir à traiter.

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See detailEditing Thomas More’s Utopia (Louvain, T. Martinus, 1516): an example of Erasmian networks in action
Adam, Renaud ULg

Conference (2016, August 19)

Theodoricus Martinus Alostensis published the editio princeps of Thomas More’s Utopia at the end of the year 1516 in Louvain. Why did the English humanist give his text to this printer and not to someone ... [more ▼]

Theodoricus Martinus Alostensis published the editio princeps of Thomas More’s Utopia at the end of the year 1516 in Louvain. Why did the English humanist give his text to this printer and not to someone like Johann Froben in Basel or Jodocus Badius Ascensius in Paris? In fact, Thomas More didn’t choose Theodoricus Martinus randomly, but had been guided by his friend Erasmus, who was living in Brabant at that time and was working directly with this printer. The humanist from Rotterdam then instructed his circle to monitor closely the printing of the text, to do the proofreading and to direct the publishing. He involved in this process his friend Petrus Aegidius – clerk of city of Antwerp and close friend of both More and Erasmus – and Gerardus Geldenhower. By studying the correspondence of Erasmus, we will first rebuild the process of printing the first edition of the Utopia. This will also be an opportunity to discover the role and the importance of a network of humanists within the workshop of a printer. Without this, Theodoricus Martinus would never have been able to build his identity as a “printer humanist” and to transform his workshop into one of the leading places for the diffusion of humanism in the Low Countries in the first decades of the 16th century. [less ▲]

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See detailAspects fonciers de l'exil des religieux français en Belgique (1901-1914)
Murez, Jean-Baptiste ULg

Conference (2016, August 19)

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See detailLes enquêtes de terrain du Musée de la Vie wallonne : Un outil au service de la collecte de la mémoire, des savoirs et savoir-faire régionaux
Postula, Jean-Louis ULg

Conference (2016, August 19)

Débutées dans les années 1920, la réalisation de reportages sur le terrain puis la diffusion régulière des résultats de ces recherches ont été pendant longtemps l’un des traits distinctifs de la fonction ... [more ▼]

Débutées dans les années 1920, la réalisation de reportages sur le terrain puis la diffusion régulière des résultats de ces recherches ont été pendant longtemps l’un des traits distinctifs de la fonction scientifique mise en œuvre au Musée de la Vie wallonne. Jusqu’à la fin des années 1980 environ, les activités du Service des enquêtes ont permis au Musée de devenir le conservatoire d’une collection unique en son genre de documents d’archives multimédia – photographies, films et sons. La rénovation du Musée à partir de 2004, assortie de la réorientation de son projet muséal vers le modèle du « musée de société », a été l’occasion d’investir à nouveau du temps et des moyens dans la relance de cette riche tradition d’enquêtes ethnographiques. Grâce à de nombreux exemples, cette communication ambitionne de montrer l’évolution des terrains d’enquêtes du Musée, qui privilégie désormais l’exploration de nouvelles thématiques liées aux enjeux contemporains de nos sociétés urbaines, industrielles et post-industrielles. Oscillant entre continuité et renouvellement, les enquêtes du Musée de la Vie wallonne permettent à l’institution de se profiler comme un contributeur influent à la préservation du patrimoine culturel immatériel de notre région. [less ▲]

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See detailAggiornamento historiographique sur Godefroid Kurth (XIXème siècle-2016)
Genin, Vincent ULg

Conference (2016, August 19)

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See detailTwo-Parameter Kinematic Approach for Shear Strength of Deep Concrete Beams with Internal FRP Reinforcement
Mihaylov, Boyan ULg

in Journal of Composites for Construction (2016), DOI: 10.1061/(ASCE)CC.1943-5614 .0000747

Tests of deep concrete beams with internal fiber-reinforced polymer (FRP) reinforcement have shown that such members can exhibit lower shear strength than members with conventional steel reinforcement. To ... [more ▼]

Tests of deep concrete beams with internal fiber-reinforced polymer (FRP) reinforcement have shown that such members can exhibit lower shear strength than members with conventional steel reinforcement. To model this effect, the current paper proposes an approach based on a two-parameter kinematic theory (2PKT) for conventional deep beams. The 2PKT is built on a kinematic model with two degrees of freedom that describes the deformation patterns of cracked beams. Using this theory shows that large strains in FRP longitudinal reinforcement result in reduced shear resistance of the critical loading zones (CLZ) of deep beams. The original 2PKT is therefore modified by introducing a reduction factor for the shear carried by the CLZ. The extended 2PKT approach is then applied to a database of 39 tests of FRP-reinforced deep beams from the literature, resulting in an average shear strength experimental-to-predicted ratio of 1.06 and a coefficient of variation of 18.3%. The results show that the 2PKT adequately captures the effects of the stiffness of the reinforcement, section depth, concrete strength, and shear-span-to-depth ratio on the shear strength of FRP-reinforced deep beams. [less ▲]

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See detailBIG NOISE - Un orchestre belge- Un nouvel hommage, en live, au JAZZ style NEW ORLEANS
Sacré, Robert ULg

E-print/Working paper (2016)

Compte Rendu du 3è opus du groupe BIG NOISE, un orchestre belge qui remet à l'honneur le jazz traditionnel style NEW ORLEANS ( album IGLOO RECORDS)

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See detailLe projet EpistolART à la lumière de certaines lettres évoquant Léonard de Vinci
Delfosse, Annick ULg; Fagnart, Laure ULg

Conference (2016, August 19)

Le projet EpistolART – dont nous sommes co-promotrices, avec Paola Moreno et Dominique Allart – explore le matériau épistolaire artistique de la première modernité. Dans ce cadre, le "Carteggio inedito ... [more ▼]

Le projet EpistolART – dont nous sommes co-promotrices, avec Paola Moreno et Dominique Allart – explore le matériau épistolaire artistique de la première modernité. Dans ce cadre, le "Carteggio inedito d’artisti dei secoli XIV, XV, XVI" que Johann Wilhelm Gaye a publié en 1839-1840 constitue l’un de nos corpus privilégiés. Méritoire, le travail de Gaye n’en demeure pas moins dépassé : aussi l’équipe EpistolART a-t-elle entrepris d'éditer à nouveaux frais ce corpus, en suivant les critères de la philologie, de la codicologie et de la paléographie moderne, et de reverser cette édition dans une base de données en ligne. Durant notre intervention, nous voudrions d’abord vous présenter la base de données EpistolART (travail éditorial, heuristique, conception de la base de données) puis nous arrêter sur quelques lettres évoquant Léonard de Vinci dont les membres du projet EpistolART proposent une nouvelle édition. Ces missives constituent des documents extrêmement précieux pour écrire l’histoire des relations entre le maître florentin et la France, spécialement avec le roi Louis XII, qui appelle l’artiste à son service en 1506. Si les historiens qui se sont penchés sur la vie et l’œuvre de Léonard de Vinci connaissent ces correspondances, rares sont ceux qui ont recouru aux documents épistolaires originaux. Or, ce n’est qu’au départ d’un texte sûr, appréhendé tant dans sa matérialité que dans ses contenus, que peuvent se déployer des analyses scientifiquement fondées. [less ▲]

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See detailMME mutation in dominant spinocerebellar ataxia with neuropathy (SCA43)
Depondt; Donatello, Simona; Rai, Myriam et al

in Neurology genetics (2016)

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See detailIncorporation of iron and organic matter into young Antarctic sea ice during its initial growth stages
Janssens, Julie; Meiners, Klaus M.; Tison, Jean-Louis et al

in Elementa: Science of the Anthropocene (2016), 4(1), 000123

This study reports concentrations of iron (Fe) and organic matter in young Antarctic pack ice and during its initial growth stages in situ. Although the importance of sea ice as an Fe reservoir for ... [more ▼]

This study reports concentrations of iron (Fe) and organic matter in young Antarctic pack ice and during its initial growth stages in situ. Although the importance of sea ice as an Fe reservoir for oceanic waters of the Southern Ocean has been clearly established, the processes leading to the enrichment of Fe in sea ice have yet to be investigated and quantified. We conducted two in situ sea-ice growth experiments during a winter cruise in the Weddell Sea. Our aim was to improve the understanding of the processes responsible for the accumulation of dissolved Fe (DFe) and particulate Fe (PFe) in sea ice, and of particulate organic carbon and nitrogen, dissolved organic carbon, extracellular polymeric substances, inorganic macro-nutrients (silicic acid, nitrate and nitrite, phosphate and ammonium), chlorophyll a and bacteria. Enrichment indices, calculated for natural young ice and ice newly formed in situ, indicate that during Antarctic winter all of the measured forms of particulate matter were enriched in sea ice compared to underlying seawater, and that enrichment started from the initial stages of sea-ice formation. Some dissolved material (DFe and ammonium) was also enriched in the ice but at lower enrichment indices than the particulate phase, suggesting that size is a key factor for the incorporation of impurities in sea ice. Low chlorophyll a concentrations and the fit of the macro-nutrients (with the exception of ammonium) with their theoretical dilution lines indicated low biological activity in the ice. From these and additional results we conclude that physical processes are the dominant mechanisms leading to the enrichment of DFe, PFe, organic matter and bacteria in young sea ice, and that PFe and DFe are decoupled during sea-ice formation. Our study thus provides unique quantitative insight into the initial incorporation of impurities, in particular DFe and PFe, into Antarctic sea ice. [less ▲]

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