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See detailNew mass spectrometry based methodology to sequence a whole snake venom
Echterbille, Julien ULg; Boulanger, Madeleine; Degueldre, Michel ULg et al

Poster (2014, February 10)

Animal venoms are complex chemical cocktails, comprising wide ranges of biologically active reticulated peptides that target with high selectivity and efficacy varieties of membrane receptors. Assuming ... [more ▼]

Animal venoms are complex chemical cocktails, comprising wide ranges of biologically active reticulated peptides that target with high selectivity and efficacy varieties of membrane receptors. Assuming the fact that each of the 170,000 venomous species reported can produce more than 250 bioactive toxins, at least 40,000,000 bioactive peptides and proteins may be discovered. Among the four described species of mambas, Eastern Jameson’s mamba (Dendroaspis jamesonii kaimosae) venom is the less characterized since only 9 peptides are referenced in database. This work aims at developing a new strategy devoted to the deep analysis of animal venoms. Our approach consists in a first separation of the venom using cation exchange chromatography. Each primary fraction is then purified a second time by classical RP-HPLC. A total of 328 fractions, containing amongst 1 and 4 toxins, are finally collected. MALDI-MS analysis of each fraction is done in order (1) to obtain information about masses and (2) to obtain sequences of toxins thanks to MALDI-In Source Decay (ISD) dissociation coupled with on MALDI target plate reduction of the peptides. ISD has already been demonstrated efficient for toxin sequencing, and especially when using 1,5-DAN as reducing matrix. ISD yields to sequences that cover more than 50% of peptide sequences by series of singly charged c-type ions. Thanks to this methodology, we were able to obtain 85% of satisfactory results i.e. spectra giving quite long tags of amino acids (up to 20 residues). As a way to validate our method, a tag coming from ISD spectrum interpretation has found a match in database for an Eastern Jameson’s mamba toxin. The global sequence has then been obtained by extrapolation on the ISD spectrum. Since ISD spectra are simpler than classical MS/MS spectra, automation of spectra interpretation, difficult with other fragmentation techniques (CID, ETD…), is implementable. In the near future, sequences obtained with this approach will be used to direct tests of biological activity through sequence homologies with already known ligands for different kinds of membrane receptors. [less ▲]

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See detailMolecular dynamic simulation of the cyclic lipodepsipeptide Pseudodesmin A self-assembly
Crowet, Jean-Marc ULg; Sinnaeve, Davy; Fehér, Krisztina et al

Conference (2014, February 10)

Pseudodesmine A is a cyclic lipodepsipeptide of nine residues which presents a moderate antibacterial activity and whose structure has been resolved by X-ray and NMR1,2. In acetonitrile, Pseudodesmine A ... [more ▼]

Pseudodesmine A is a cyclic lipodepsipeptide of nine residues which presents a moderate antibacterial activity and whose structure has been resolved by X-ray and NMR1,2. In acetonitrile, Pseudodesmine A is monomeric while in chloroform it has the same structure but assemble in a supramolecular complex. This structure could associate with membranes and be responsible of the biological activity of this peptide. Comparison of the NMR data between the two solvents has given indications on the intermolecular contacts that arise in chloroform and a model for the self association was proposed2,3. To study in more details this assembly, molecular dynamics have been carried on. In acetonitrile, the peptide show transient interactions while in chlorofom interactions between monomers was always observed. As stated in Sinnaeve et al. in 2009, these interactions arise mainly between the backbone protons of the LEU1 and the GLN2, the GLN2 sidechain and the loop located on the opposite end of the monomer structure. From 10 simulations of dimerization, hydrogen bonds were followed and specific interaction patterns were identified regarding the hydrogen bonds formed. The peptide interactions are mainly described by 13 interaction patterns; 8 with the peptides in a linear configuration, 1 perpendicular and 4 with peptides side by side. The patterns are characterized by 2 to 4 hydrogen bonds. From the linear dimer, it is possible to reconstruct filaments and, by combining a linear and a lateral dimer, it is possible to build fibrils with multi filaments, as expected in the NMR derived model. Besides, the perpendicular dimer can gives peptide rings that can also explain the potential ability of this peptide to form ion pores in membranes. 1. Sinnaeve, D., Michaux, C., Van hemel, J., Vandenkerckhove, J., Peys, E., Borremans, F. a. M., Sas, B., Wouters, J. and Martins, J. C. Tetrahedron 2009, 65, 4173–4181. 2. Sinnaeve, D., Hendrickx, P. M. S., Van Hemel, J., Peys, E., Kieffer, B. and Martins, J. C. Chemistry (Weinheim an der Bergstrasse, Germany) 2009, 15, 12653–62. 3. Sinnaeve, D., Delsuc, M.-A., Martins, J. C. and Kieffer, B. Chemical Science 2012, 3, 1284. [less ▲]

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See detailAllo- and autoinhibition in barley and great brome: a laboratory study
Bouhaouel, Imen ULg; Gfeller, Aurélie; Fauconnier, Marie-Laure ULg et al

Conference (2014, February 08)

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See detailAccumulation primitive et dictature du prolétariat. Une lecture conjoncturelle du dernier Althusser
Mancuso, Eva ULg; Bruschi, Fabio

Scientific conference (2014, February 08)

Detailed reference viewed: 13 (1 ULg)
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See detailINFLUENCE OF STEAM EXPLOSION ON THECRYSTALLINITY OF CELLULOSE FIBER
Jacquet, Nicolas ULg; Vanderghem, Caroline ULg; Danthine, Sabine ULg et al

Poster (2014, February 07)

The aim of the present study is to compare the effect of different steam explosion treatments on crystallinity properties of a pure bleached cellulose. Steam explosion process is composed of two distinct ... [more ▼]

The aim of the present study is to compare the effect of different steam explosion treatments on crystallinity properties of a pure bleached cellulose. Steam explosion process is composed of two distinct stages: vapocracking and explosive decompression. The treatment intensities is determined by a severity factor, established by a correlation between temperature process and retention time. The results show that steam explosion treatment has an impact on the crystallinity properties of pure cellulose fiber. When the severity factor is below 5.2, an increase of the overall crystallinity of the samples is observed with the treatment intensities. For higher intensities, a significant thermal degradation of cellulose lead to an important change in substrate composition, which lead to a further decrease of cellulose crystallinity. [less ▲]

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See detailProfiles of the volatile organic compounds emitted by the masses of Abies nordmanniana somatic embryos at maintenance and maturation stages
Druart, Philippe; Michels, Franck ULg; Misson, Jean-Pierre et al

Poster (2014, February 07)

Detailed reference viewed: 23 (1 ULg)
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See detailPrerequisites for a Black locust genomic selection program
Verdu, Cindy ULg; Mengal, Coralie ULg; Henrotay, Frédéric ULg et al

Poster (2014, February 07)

The use of renewable resources as an alternative to fossil fuels has become a priority. Efficient use of forest as a resource for energy and green chemistry purposes require the development of suitable ... [more ▼]

The use of renewable resources as an alternative to fossil fuels has become a priority. Efficient use of forest as a resource for energy and green chemistry purposes require the development of suitable selected genotypes that are competitive and ready to meet the challenges of global change. In this context, the black locust, Robinia pseudoacacia L., is a very promising species which has many advantages in the context of current global change: high phenotypic plasticity, drought resistance, high biomass production and fixation of atmospheric nitrogen. The genetic improvement of woody species using traditional methods can take between 20 to 30 years. These times can be greatly reduced with the development of new selection methods such as genomic selection. Before starting a genomic selection program, it is necessary to 1) develop new molecular markers to achieve a very dense genetic map for genomic selection, 2) study the genetic diversity of the species present in Belgium and compare it with that of the native area, 3) study the structure and the relatedness of different populations, 4) establish a core collection gathering the most genetically diverse individuals, and 5) as black locust is an exotic species, verify its invasiveness in Belgium. These 5 steps will be detailed and the first results obtained will be discussed. [less ▲]

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See detailUse of Near Infrared Hyperspectral Imaging (NIR-HSI) and chemometric tools to dicriminate wheat roots and straws in soil
Eylenbosch, Damien ULg; Fernandez Pierna, Juan Antonio; Baeten, Vincent et al

Poster (2014, February 07)

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See detailAnalyzing relationships between cattle grazing behavior and pasture attributes using the inertial measurement unit of a mobile phone.
Andriamandroso, Andriamasinoro ULg; Lebeau, Frédéric ULg; Bindelle, Jérôme ULg

Poster (2014, February 07)

The recent technological developments are boosting the opportunities of accurate method to monitor resource use efficiency in agriculture and in their wake, precision livestock farming (PLF) has ... [more ▼]

The recent technological developments are boosting the opportunities of accurate method to monitor resource use efficiency in agriculture and in their wake, precision livestock farming (PLF) has experienced huge developments over the past decade. These developments focus on the optimization of individual performances of farm animals as opposed to herd management. The aim of this paper is to explore a method to detect accurately and to analyze changes in cattle's behaviors on pasture during grazing time using signals from the inertial measurement unit (IMU) of mobile devices as a possible tool to manage individual grazing behavior. Commercial iPhones or iPods, which include a 3-axis accelerometer, a gyroscope and a GPS sensor, are fitted on a halter and placed on the neck of grazing cows. The acquired IMU data are recovered using an open source application (Sensor Data, Wavefrontlabs) and analyzed in a “white-box” model of the cows’ movements. First results using time-domain analysis allowed the detection of grazing behaviors showed accuracies ranging between 84% and 96%, attesting the relevancy of the method. Refined signal processing method will improve the detection but will also inform more about the relative link between the behaviors and the pasture attributes such as sward height, composition and nutritive value. [less ▲]

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See detailRobot weed killers - no pain more gain
Krishna Moorthy Parvathi, Sruthi Moorthy ULg; Mercatoris, Benoît ULg; Boigelot, Bernard ULg

Poster (2014, February 07)

Weed destruction plays a significant role in crop production, and its automation has both economic and environmental benefits by minimizing the usage of chemicals in the fields. Our aim is to design a ... [more ▼]

Weed destruction plays a significant role in crop production, and its automation has both economic and environmental benefits by minimizing the usage of chemicals in the fields. Our aim is to design a small low-cost versatile robot allowing the destruction of weeds that lie between the crop rows by navigating in the field autonomously. Major challenges foreseen are: mapping the unknown geometry of the field, high-level planning of efficient and complete coverage of the field, and controlling the low-level operations of the robot. Traditionally, sensors like odometer have been used for localisation of robots but without much success in real-world scenarios. Specialized sensors like cameras will therefore be investigated and the plethora of image recognition algorithms will be explored and fine-tuned to enable Simultaneous Localisation And Mapping (SLAM) even on resource constrained robotic platforms. Vision-based localisation is not always viable because of the varying weather conditions of the environment and to overcome that, intelligent stochastic data fusion and machine learning algorithms will be utilized to combine data from heterogenous sensor. The image sensors for localisation will be re-used to differentiate crop rows from the weeds, which are cut when they grow. Finally, logics and reinforcement learning techniques will be explored, to exploit the generated map of the field and other sensorial information, to efficiently plan and execute weed elimination. [less ▲]

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See detailImpact of abiotic stresses on volatile organic compound production of field crops and grasslands
Digrado, Anthony ULg; Mozaffar, Ahsan ULg; Bachy, Aurélie ULg et al

Poster (2014, February 07)

Abiotic and biotic stresses are known to alter biogenic volatile organic compound (BVOC) emission from plants. With the climate and global change, BVOC emissions are likely to increase. This increase on ... [more ▼]

Abiotic and biotic stresses are known to alter biogenic volatile organic compound (BVOC) emission from plants. With the climate and global change, BVOC emissions are likely to increase. This increase on BVOC emissions could be driven by many environmental parameters like temperature, ozone and light availability for photosynthesis although it is still difficult to predict the impact of some environmental parameters, environmental controls on BVOC emission being species and BVOC-dependent. These BVOC are involved in a wide range of interactions of plants with their environment and these interactions could be affected by the global change. Moreover, BVOC also play a key role in the atmospheric chemistry and may contribute to ozone formation and an increase in methane lifetime, strengthening the global change. Yet, due to technical limitation, there are few studies examining the impact of multiple co-occurring stresses on BVOC emission at the ecosystem level although stress combination is probably more ecologically realistic in field. In the CROSTVOC (for CROp STress VOC) project, the impact of abiotic stresses (e.g. heat, drought, ozone and grazing) on BVOC emission will be investigated for field crops (maize and wheat) and grassland both at the ecosystem and plant scale. [less ▲]

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See detailPrise en compte de la charge vocale dans l’évaluation et le traitement de la dysphonie
REMACLE, Angélique ULg

Scientific conference (2014, February 07)

Detailed reference viewed: 12 (0 ULg)
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See detailIs the behaviour of grazing cattle influenced by the presence of a hedge in the pasture?
Vandermeulen, Sophie ULg; Yando, Emile; Marche, Christian et al

Poster (2014, February 07)

The interest in using shrubs and trees as forage for cattle is raising in temperate regions, along with the environmental measures promoting hedges along pasture. This study aimed at determining the ... [more ▼]

The interest in using shrubs and trees as forage for cattle is raising in temperate regions, along with the environmental measures promoting hedges along pasture. This study aimed at determining the influence of the presence of a woody hedge on grazing cattle behavior as well as the evolution of this behavior during the grazing season. Twelve heifers were set to graze a standard ryegrass and clover meadow during the grazing season of 2013. The animals were divided in 2 groups : one with a free access to a hedge composed by 10 temperate shrub and tree species, the other with no access to a hedge (control). Their behavior (grazing, browsing and other activities) was monitored during 14h d-1 replicated 3d week-1 during 3 periods (May, July and September). Results suggest that both season and presence of a hedge have an influence on the behavior of heifers. Grazing, resting and rumination varied between the two groups in each period (P<0.05). The time spent browsing was influenced by the season (P<0.001); the heifers with an access to the hedge could browse 19.3% of the total time in May against 5.9 and 5.4% in July and September. Both control and experimental heifers grazed more with the progressing season, but the animals without the hedge grazed always more than the heifers that could graze and browse (49.6 vs 43.0%). In conclusion, browsing woody species represents a significant part of the time spent for forage ingestion, and variation in this behavior over the whole grazing season requires to investigate the influence of both pasture and browse availability and composition. [less ▲]

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See detailSreening of essential oils against rice pathogens isolated from Madagascar
Mamiharisoa Razanakoto, Léa ULg; De Clerck, Caroline ULg; Massart, Sébastien ULg et al

Poster (2014, February 07)

Four predominant isolated pathogens have been identified during survey of rice diseases in three regions in Madagascar. These pathogens were the bacteria Xanthomonas oryzae pv. oryzae and Pseudomonas ... [more ▼]

Four predominant isolated pathogens have been identified during survey of rice diseases in three regions in Madagascar. These pathogens were the bacteria Xanthomonas oryzae pv. oryzae and Pseudomonas fuscovaginae and the fungi Alternaria sp. and Curvularia lunata. These pathogens are causing significant yield reduction on rice, which is the staple food in this country. After the identification of those pathogens, the objective of this work was to identify appropriate control measures against them. Plants extracts has been traditionally used to control diseases in other plant species. Nowadays, the problem of residues of phytopharmaceutical product, reinforce the need for research on the development of natural plant extracts to control plant diseases. Madagascar has many endemic plant of interest and we have evaluated the antimicrobial properties of essential oils from Malagasy plants. The main object of this study is the in-vitro screening of essential oils to control the pathogens isolated on rice in Madagascar. Thirty nine essentials oils from Madagascar had been tested. Activity of essentials oils on Pseudomonas fuscovaginae, Alternaria sp. and Curvularia lunata had been tested using broth media and on agar medium for Xanthomonas oryzae pv. oryzae. The growth of the pathogen (bacteria and conidia) in the presence of the essential oil was evaluated and compared to a control. Nine of the 39 essentials oils showed interesting antimicrobial activity with an efficiency more than 70% against at least one bacterial pathogen. One essential oil inhibited the growth of the two pathogens tested. For fungal pathogens, three essential oils presented an efficiency of more than 70% against at least one fungal pathogen. These results confirm that some essential oils present an interesting antimicrobial activity. The in-situ confirmation on plant of this activity is currently ongoing. [less ▲]

Detailed reference viewed: 14 (0 ULg)