Last 7 days
Bookmark and Share    
Full Text
See detailEU and Brazil : partners or competitors ?
Santander, Sébastian ULg

Scientific conference (2014, April 03)

Detailed reference viewed: 10 (0 ULg)
Full Text
Peer Reviewed
See detailRates of CTL Killing in Persistent Viral Infection In Vivo
Elemans, Marjet; Florins, Arnaud; Willems, Luc ULg et al

in PLoS Computational Biology (2014), 4

The CD8+ cytotoxic T lymphocyte (CTL) response is an important defence against viral invasion. Although CTL-mediated cytotoxicity has been widely studied for many years, the rate at which virus-infected ... [more ▼]

The CD8+ cytotoxic T lymphocyte (CTL) response is an important defence against viral invasion. Although CTL-mediated cytotoxicity has been widely studied for many years, the rate at which virus-infected cells are killed in vivo by the CTL response is poorly understood. To date the rate of CTL killing in vivo has been estimated for three virus infections but the estimates differ considerably, and killing of HIV-1-infected cells was unexpectedly low. This raises questions about the typical anti-viral capability of CTL and whether CTL killing is abnormally low in HIV-1. We estimated the rate of killing of infected cells by CD8+ T cells in two distinct persistent virus infections: sheep infected with Bovine Leukemia Virus (BLV) and humans infected with Human T Lymphotropic Virus type 1 (HTLV-1) which together with existing data allows us to study a total of five viruses in parallel. Although both BLV and HTLV-1 infection are characterised by large expansions of chronically activated CTL with immediate effector function ex vivo and no evidence of overt immune suppression, our estimates are at the lower end of the reported range. This enables us to put current estimates into perspective and shows that CTL killing of HIV-infected cells may not be atypically low. The estimates at the higher end of the range are obtained in more manipulated systems and may thus represent the potential rather than the realised CTL efficiency. [less ▲]

Detailed reference viewed: 4 (0 ULg)
Full Text
Peer Reviewed
See detailUse of on-line flow cytometry for the characterization of microbial stress dynamics during the bioprocess
Brognaux, Alison ULg; Han, Shanshan; Sorensen, Soren et al

Conference (2014, April 03)

Microbial cell population heterogeneity is now recognized as a major source of issues for the development and optimization of bioprocesses. Flow cytometry is a very powerful tool for the follow up of ... [more ▼]

Microbial cell population heterogeneity is now recognized as a major source of issues for the development and optimization of bioprocesses. Flow cytometry is a very powerful tool for the follow up of physiological properties of microbial cells in process-related conditions at the single cell level, and can be used to study the dynamics of segregation directly in bioreactors. In this context, specific interfaces have been developed in order to connect flow cytometer (FC) directly on bioreactor for automated analyses. In this work, we propose a simplified version of such interface and demonstrated its usefulness for multiplexed experiments. This automated FC system has been tested for the follow up of the dynamics of an E. coli pfis::gfpAAV fluorescent bio-reporter and its PI uptake, correlated with membrane permeability. This bioreporter is composed of a fis promoter, a growth dependent promoter-indicator of the nutrient status of cells, fused to a gene expressing an unstable variant of GFP. The results obtained showed that the dynamics of the GFP synthesis is complex and can be attributed to a complex set of biological parameters. Segregation in the membrane permeability has been noticed. This work demonstrates that a simplified version of on-line FC can be used at the process level for the investigation of the dynamics of complex physiological mechanisms. [less ▲]

Detailed reference viewed: 27 (9 ULg)
Peer Reviewed
See detailNo simian Plasmodium detected in populations living in the equatorial rainy forest of the Democratic Republic of Congo
Mvumbi makaba, Dieudonné; Bobanga Lengu, Thierry; Kayembe Ntumba, Jean-Marie et al

Poster (2014, April 03)

Background Malaria remains the most deadly parasitic disease to date, especially in sub-Saharan Africa, which comprises the majority of cases collected per year. It has long been accepted that four ... [more ▼]

Background Malaria remains the most deadly parasitic disease to date, especially in sub-Saharan Africa, which comprises the majority of cases collected per year. It has long been accepted that four species of Plasmodium (P. falciparum, P. vivax, P. malariae and P. ovale) were responsible for the disease in humans. But quite recently, a fifth species, Plasmodium Knowlesi, has been identified as naturally infecting humans. Indeed, known for decades as naturally parasitizing the monkey Macaca fascicularis, P. knowlesi has long been confused, in terms of its evolutionary stage, with P. malariae or P. falciparum, which it resembles morphologically and it was not possible to properly differentiate them until the advent of molecular biology. To date, P. Knowlesi has only been identified in Southeast Asia and a similar phenomenon of natural transmission of simian plasmodium to humans has not been reported elsewhere. We therefore conducted this study to investigate the possible transmission of simian plasmodium to humans in populations living near the rainforest of the Democratic Republic of Congo (DRC) where several species of primates lives. Methods & Materials Three villages (Wenji-Secli, Bongonde, and Bolenge) in the Province of Ecuador (North-eastern DRC) were selected because of their geographical location. Blood samples spotted on filter paper were collected from 100 people randomly taken in each village. Two successive RT- PCR were performed. A first one using a single probe able to diagnose all plasmodium spp. and a second using four species-specific probes for the diagnosis of the four conventional human plasmodium species. Positivity in the first RT- PCR with negativity in the second RT- PCR would suggest the presence of plasmodium species other than the four conventional. Results P. falciparum was correctly identified in 44.6 % of samples. No other species of human plasmodium or not has been identified. Conclusion This preliminary study did not detect the presence of simian plasmodium in human populations living in the rainforest of the DRC. Studies with larger samples and with more advanced techniques should still be conducted. Keywords: Malaria, simian plasmodium, DR Congo [less ▲]

Detailed reference viewed: 47 (2 ULg)
See detailLa confiance comme processus relationnel. Une tentative de modélisation
Dethier, Baptiste ULg

Scientific conference (2014, April 03)

Detailed reference viewed: 20 (1 ULg)
Full Text
See detailCaractérisation minéralogique, géochimique et géotechnique des matières premières utilisées en poterie dans le site d’Agafay, Marrakech, Maroc.
El Boudour El Idrissi, Hicham; Daoudi, Lahcen; El Ouahabi, Meriam ULg et al

Conference (2014, April 03)

Le site potier d’Agafay est localisé à 20 km au S-W de la ville de Marrakech, à l’ouest de l’oued N’Fis. L’activité potière y a débuté par transfert du savoir-faire à partir du village de Tamsloht vers ... [more ▼]

Le site potier d’Agafay est localisé à 20 km au S-W de la ville de Marrakech, à l’ouest de l’oued N’Fis. L’activité potière y a débuté par transfert du savoir-faire à partir du village de Tamsloht vers 1940 apr. J.-C. Le site comporte environ 90 ateliers où sont actifs 240 artisans. Les principales pièces produites sont les gasriyas (i.e., plats traditionnels de couscous et de modelage de pâtes de pains) et les briques des grandes murailles traditionnelles. Les matières premières utilisées, brutes ou en mélange, sont issues des terres agricoles locales (Ag1 et Ag5) pour les gasriyas et des dépôts actuels du oued N’Fis (Ag7) pour les briques. Les problématiques rencontrées dans ce site concernent les deux types de produits, gasriyas et briques. Dans le premier cas, des grains de la chaux gonflent dans les pièces après le contact prolongé des pièces produites avec l’air. Ce gonflement engendre des décollements dans les tessons et nuit à la qualité du produit final. Dans le deuxième cas, la distribution non homogène de la chaleur à l’intérieur des fours traditionnels utilisés engendre une très grande variation de couleur dans les briques, elles sont généralement tordues et certaines fondent totalement. Notre projet vise à une caractérisation scientifique des différentes phases de fabrication afin de remédier à ces problèmes. Les résultats montrent que la composition chimique est surtout silico-alumineuse (55 à 61 % de SiO2 ; 16 à 20% d’Al2O3), la teneur en Fe2O3 se situe entre 6 et 8 %, celle de CaO ne dépasse pas 6% pour les pâtes des gasriyas et atteint 10 % pour les pâtes des briques. La teneur en carbonates mesurée par le calcimètre de Bernard varie entre 5 et 14 %. La teneur en matière organique varie entre 5 et 7,5 %. L’indice de plasticité oscille entre 16 et 17,5. Pour des teneurs en eau proches de la limite de plasticité le degré de retrait au séchage des gasriyas est de l’ordre de 5 %, il est plus élevé pour les briques (8%) ce qui nécessite une plus grande maitrise du processus de séchage pour éviter que les pièces ne se tordent pas. La granulométrie des pâtes utilisées pour les gasriyas a montré une teneur moyenne de 50 % pour les argiles, 32 % pour les limons et 18 % pour les sables. Les briques comportent une proportion égale des trois classes granulométriques. La minéralogie de l’ensemble des pâtes est formée de quartz (< 27%), plagioclase (13%), calcite (7 à 9%), dolomite (5%) et argile (50%, 34% et 34% pour Ag1, Ag5 et Ag7, respectivement). Le feldspath potassique est présent uniquement dans l’échantillon Ag7. Concernant la fraction argileuse, elle est surtout constituée d’illite pour l’ensemble des pâtes, associée à des interstratifiés irréguliers 14c-14s (13 %) pour la pâte des briques, et une teneur de 11 % de smectite pour la pâte des gasriyas (Ag5). La mesure de la vitesse sonique a montré que les pièces formées par pression aboutissent à une porosité moindre que celle des pièces produites par boudinage. La densité des pièces produites reste semblable pour les cuissons à 950°C et 1050°C. Le plus bas degré de porosité ainsi que les plus grandes valeurs de résistance mécanique à la compression ont été enregistrés sur les pièces cuites à 1050 °C. Les contraintes maximales atteintes se situent entre 51 et 59 MPa pour les gasriyas et 24,5 MPa pour les briques. Quant à la résistance mécanique à la flexion, elle varie entre 17,4 et 20,3 N/mm² pour les gasriyas et 9,4 N/mm² pour les briques. L’analyse DRX des pièces cuites à 1050 °C a montré la disparition totale des carbonates et des argiles à cette température, accompagnée de l’apparition de la géhelénite, cristobalite, anorthoclase, diopside, spinelle et hématite. A l’issue de cette étude, nous recommandons un tamisage humide à 1 mm pour réduire l’effet des gros grains de la chaux, une cuisson par palier jusqu’à 1050°C est également nécessaire pour comprendre les transformations des phases minéralogiques et vitreuses. Finalement, l’utilisation des fours équipés d’une sonde pour garantir une meilleure distribution de la chaleur. [less ▲]

Detailed reference viewed: 40 (8 ULg)
Full Text
Peer Reviewed
See detailNest grouping patterns of bonobos (Pan paniscus) in relation to fruit availability in a forest-savannah mosaic
Serckx, Adeline ULg; Huynen, Marie-Claude ULg; Bastin, Jean-François ULg et al

in PLoS ONE (2014)

A topic of major interest in socio-ecology is the comparison of chimpanzees and bonobos’ grouping patterns. Numerous studies have highlighted the impact of social and environmental factors on the ... [more ▼]

A topic of major interest in socio-ecology is the comparison of chimpanzees and bonobos’ grouping patterns. Numerous studies have highlighted the impact of social and environmental factors on the different evolution in group cohesion seen in these sister species. We are still lacking, however, key information about bonobo social traits across their habitat range, in order to make accurate inter-species comparisons. In this study we investigated bonobo social cohesiveness at nesting sites depending on fruit availability in the forest-savannah mosaic of western Democratic Republic of Congo (DRC), a bonobo habitat which has received little attention from researchers and is characterized by high food resource variation within years. We collected data on two bonobo communities. Nest counts at nesting sites were used as a proxy for night grouping patterns and were analysed with regard to fruit availability. We also modelled bonobo population density at the site in order to investigate yearly variation. We found that one community density varied across the three years of surveys, suggesting that this bonobo community has significant variability in use of its home range. This finding highlights the importance of forest connectivity, a likely prerequisite for the ability of bonobos to adapt their ranging patterns to fruit availability changes. We found no influence of overall fruit availability on bonobo cohesiveness. Only fruit availability at the nesting sites showed a positive influence, indicating that bonobos favour food ‘hot spots’ as sleeping sites. Our findings have confirmed the results obtained from previous studies carried out in the dense tropical forests of DRC. Nevertheless, in order to clarify the impact of environmental variability on bonobo social cohesiveness, we will need to make direct observations of the apes in the forest-savannah mosaic as well as make comparisons across the entirety of the bonobos’ range using systematic methodology. [less ▲]

Detailed reference viewed: 36 (8 ULg)
Full Text
Peer Reviewed
See detailBayesian inference of a dynamic vegetation model for grassland
Minet, Julien ULg; Laloy, Eric; Tychon, Bernard ULg et al

Conference (2014, April 02)

As a part of the MACSUR task L2.4, we probabilistically calibrated the CARAIB dynamic vegetation model by Markov chain Monte Carlo (MCMC) simulation with the DREAMZS sampler.. CARAIB is a mechanistic ... [more ▼]

As a part of the MACSUR task L2.4, we probabilistically calibrated the CARAIB dynamic vegetation model by Markov chain Monte Carlo (MCMC) simulation with the DREAMZS sampler.. CARAIB is a mechanistic model that calculates the carbon assimilation of the vegetation as a function of the soil and climatic conditions, and can thus be used for simulating grassland production under cutting or grazing management. Bayesian model inversion was performed at 4 grassland sites across Europe: Oensingen, CH; Grillenburg, DE; Laqueuille, FR and Monte-Bodone, IT. Four daily measured variables from these sites: the Gross Primary Productivity (GPP), Net Ecosystem Exchange (NEE), Evapotranspiration (ET) and Soil Water Content (SWC) were used to sample 10 parameters related to rooting depth, stomatal conductance, specific leaf area, carbon-nitrogen ratio and water stresses. The maximized likelihood function therefore involved four objectives, whereas the applied Bayesian framework allowed for assessing the so called parameter posterior probability density function (pdf), which quantifies model parameter uncertainty caused by measurement and model errors. Sampling trials were performed using merged data from all sites (all-sites-sampling) and for each site (site-specific sampling) separately. The derived posterior parameter pdfs from the all-sites sampling and site-specific sampling runs showed differences in relation with the specificities of each site. Analysis of these distributions also revealed model sensitivity to parameters conditioned on the measured data, as well as parameter correlations. [less ▲]

Detailed reference viewed: 21 (2 ULg)
Full Text
See detailWill top-down interactions be affacted by climate change?
Boullis, Antoine ULg; Verheggen, François ULg; Francis, Frédéric ULg

Poster (2014, April 02)

Greenhouse gas emissions are rising since the beginning of the industrial era: atmospheric carbon dioxide now exceeds 400 ppm (30% increase since the mid-1800s) and tropospheric ozone has reached 40 ppb ... [more ▼]

Greenhouse gas emissions are rising since the beginning of the industrial era: atmospheric carbon dioxide now exceeds 400 ppm (30% increase since the mid-1800s) and tropospheric ozone has reached 40 ppb in industrialized areas. According to forecasting models, these concentrations are expected to double by the end of the 21st century. To date, impacts of elevated greenhouse gases have been extensively studied in plant-insect interactions, whereas interactions between herbivores and their natural enemies have been neglected. Because most interactions between herbivorous insects and their natural enemies are orchestrated by natural molecules produced as semiochemicals, we hypothesize that modification in atmospheric gas composition will alter chemical communication in insects, and affect the performance of natural enemies to locate their prey. In this project, we shall attempt to analyze the effect of increase in greenhouse gas concentrations on insect communication, particularly in top-down interactions. The study model will consist in the interactions between the pea aphids (Acyrthosiphon pisum (Harris)) and their natural enemies Harmonia axyridis (Pallas) and Episyrphus balteatus (DeGeer). Under elevated CO2 and/or O3 concentrations, we will quantify the production of several aphid semiochemicals that are currently used as kairomones by natural enemies, including the aphid alarm pheromone E-β-farnesene and honeydew volatile compounds. Olfactometry assays will then be set up to examine the ability of natural enemies to locate their prey in the predicted atmospheric conditions of the end of the 21st century. Finally, using an Ecotron structure to be built in Gembloux Agro-Bio Tech, we will simulate a future ecosystem with the possibility to control additional biotic and abiotic parameters. [less ▲]

Detailed reference viewed: 20 (7 ULg)
Full Text
See detailBiological control of aphids in wheat and vegetable crops : a multi-approach case study in Shandong province (China)
Chevalier Mendes Lopes, Thomas ULg; Bosquée, Emilie ULg; Chen, Julian et al

Poster (2014, April 02)

This multi-approach study was conducted in the Shandong province (China) to assess the effectiveness of different biological control methods against aphids in wheat and vegetable crops. Three approaches ... [more ▼]

This multi-approach study was conducted in the Shandong province (China) to assess the effectiveness of different biological control methods against aphids in wheat and vegetable crops. Three approaches were tested: (1) wheat/oilseed rape and wheat/pea associations, (2) potatoes/peas association, and (3) E-β-farnesene (aphid alarm pheromone) releasers in squashes under plastic tunnels. Aphids and aphidophagous beneficials were monitored by observations on plants. Wheat associations and E-β-farnesene reduced aphid infestations compared with control treatments, while no effect was observed in the potatoes/peas association. Moreover, aphidophagous beneficials were significantly more abundant in the three kinds of associations compared with control treatments. Few individuals were observed on squashes. Additionally, adult aphidophagous beneficials were monitored using yellow pan traps. The parasitoid species Aphidius gifuensis (Ashmead) was prevalent, while Propylea japonica (Thunberg) and Harmonia axyridis (Pallas) were the most abundant species among predators. Globally, wheat associations were more efficient to reduce aphid infestations and attract aphidophagous beneficials to promote biological control. The trapping of natural enemies allowed to assess the diversity and abundance of aphidophagous species in this region. This study shows a beneficial effect of crop associations and E-β-farnesene on the control of aphid populations and contributes to assess the potentialities of these alternative methods to the use of insecticides. [less ▲]

Detailed reference viewed: 21 (4 ULg)
Peer Reviewed
See detailConservatoire entomologique de Gembloux Agro-Bio Tech: les coccinelles de Wallonie et de Bruxelles de 2001 à 2009
Vandereycken, Axel ULg; Fassotte, Bérénice ULg; Durieux, Delphine ULg et al

Poster (2014, April 02)

Un inventaire des Coccinellidae présents au sein de la collection de l’unité d’Entomologie fonctionnelle et évolutive (ULg - Gembloux Agro-Bio Tech) a été réalisé sur la période s’étendant de 2001 à 2009 ... [more ▼]

Un inventaire des Coccinellidae présents au sein de la collection de l’unité d’Entomologie fonctionnelle et évolutive (ULg - Gembloux Agro-Bio Tech) a été réalisé sur la période s’étendant de 2001 à 2009. Cette collection est essentiellement constituée par les récoltes des étudiants de première Bachelier de Gembloux Agro-Bio Tech. A travers ce recensement, nous avons étudié l’évolution des espèces récoltées au sein de cette famille en termes d’effectif relatif et de richesse spécifique. Notre étude s’est focalisée sur les données issues de Wallonie et de la Région de Bruxelles-Capitale, trop peu de collectes ayant été réalisées en Flandre. Parmi les individus identifiés, 27 espèces ont été observées, dont 21 appartiennent à la sous-famille des Coccinellinae, 2 à celle des Epilachninae et 4 à celle des Chilocorinae. La plupart des espèces sont communes à la Belgique. Cependant, Harmonia axyridis (Pallas 1773), espèce originaire du sud-est de l’Asie, a été introduite sur le territoire belge en 1997. Cette coccinelle exotique fait son apparition dans les récoltes de 2002 et son effectif ne cesse d’augmenter au fil des années prospectées. En parallèle à cette augmentation, une diminution de la richesse spécifique, ainsi que de l’effectif relatif d’Adalia bipunctata (L. 1758), de Propylea quatuordecimpunctata (L. 1758) et de Psyllobora vigintiduopunctata (L. 1758), est observée. [less ▲]

Detailed reference viewed: 12 (0 ULg)
Peer Reviewed
See detailImages fixes, cadre mobile. Fantômes de cinéma dans la bande dessinée expérimentale contemporaine
Belloi, Livio ULg

Conference (2014, April 02)

La présente communication porte sur la bande dessinée expérimentale contemporaine et se focalise plus particulièrement sur "Travelling Square District" (2010) du jeune dessinateur belge Greg Shaw. Objet ... [more ▼]

La présente communication porte sur la bande dessinée expérimentale contemporaine et se focalise plus particulièrement sur "Travelling Square District" (2010) du jeune dessinateur belge Greg Shaw. Objet très singulier, cet album se présente comme une expérimentation systématique autour du cadre mobile, dans un art fondé sur des successions d'images fixes. En cela, cette œuvre peut être considérée comme relevant d'une para-histoire du cinéma. [less ▲]

Detailed reference viewed: 15 (3 ULg)
See detailApport des examens biologiques en pratique allergologique
GADISSEUR, Romy ULg

Conference (2014, April 02)

Apport des examens biologiques en pratique allergologique

Detailed reference viewed: 13 (1 ULg)
Full Text
Peer Reviewed
See detailInvolved PhD research – a case study between agronomy and social sciences
Klaedtke, Stephanie ULg; Chable, Véronique; Stassart, Pierre M ULg

Conference (2014, April 02)

In research projects based on questions which emerge from practice and from interactions with different stakeholders, researchers engage with research partners in a cooperative and interdisciplinary ... [more ▼]

In research projects based on questions which emerge from practice and from interactions with different stakeholders, researchers engage with research partners in a cooperative and interdisciplinary approach. In PhD projects, including a diverse network of partners and interdisciplinarity may be challenged by the short duration of the projects and by the positivist and reductionist approaches in which students are initially trained. Based on a concrete PhD research project on small-scale organic seed production, the objective of this work is to elucidate how interdisciplinarity and an action-base can nevertheless gradually be integrated by PhD students into an approach best termed as involved research. Reflection on the roles of partners, their involvement, motivation and points of view and on the evolution of questions posed during interactions between students and stakeholders contributes to recognizing the role of each partner, situating research postures, and identifying the types of knowledge and transformations produced. While PhD students and their research projects can evolve towards more holistic and constructivist approaches, it is crucial to maintain opportunities for reflection during their research in order to realize the potentials of involving all those involved in the projects and produce effective social learning processes. [less ▲]

Detailed reference viewed: 15 (1 ULg)
Peer Reviewed
See detailDiversité et abondance entomologique en cultures maraîchères à Libreville et à Owendo (Gabon)
Bayendi-Loudit, Sandrine ULg; Poligui, René Noël ULg; Ndoutoume Ndong, Auguste et al

Conference (2014, April 02)

In order to improve knowledge of the diversity and abundances of the entomofauna occurring within market gardenings in Gabon, this study was conducted in three localities in Libreville and Owendo, from ... [more ▼]

In order to improve knowledge of the diversity and abundances of the entomofauna occurring within market gardenings in Gabon, this study was conducted in three localities in Libreville and Owendo, from July1st to August 31st, 2012. Insect collections from yellow traps and visual observations were performed weekly within plots in which grow Amaranth (Amaranthus sp., Amaranthaceae), red sorrel (Hibiscus sabdariffa L, Malavaceae), Tomato (Lycopersicon esculentum Mill, Solanaceae) and Cabbage (Brassica oleracea L, Brassicaceae). A total of 7119 insects were assessed and taxonomically classified in 8 distinct orders comprising 81 families. The study highlighted some entomologic families with agronomic interest. The main pests were represented by Aphididae (31,0%), Cecidomyiidae (5,1%), Chrysomelidae (4,0%) and Thripidae (2,0%), the useful insects (pollinators and predators) by Dolichopodidae (5,4%), Andrenidae (2,6%) Coccinellidae (1,7%), Syrphidae (0,6%), Braconidae (0,5%) and Hemerobiidae (0,1%) and the associated insects were Psychodidae (7,4%), Muscidae (5,9%), Formicidae (4,4%) and Anthomyiidae (4,2%). Entomological diversity is discussed according to the local conditions and potential for sustainable pest control encountered. [less ▲]

Detailed reference viewed: 38 (3 ULg)
Peer Reviewed
See detailA pheromone-based formulation against phytophagous pests
Fassotte, Bérénice ULg; Vandereycken, Axel ULg; Heuskin, Stéphanie ULg et al

Poster (2014, April 02)

Innovative integrated pest management methods are needed to overcome market withdrawal of synthetic pesticides. Therefore, the identification of environment-friendly bio-products carrying direct or ... [more ▼]

Innovative integrated pest management methods are needed to overcome market withdrawal of synthetic pesticides. Therefore, the identification of environment-friendly bio-products carrying direct or indirect biocide activity is one promising alternative option. Our researches focus on the identification of appropriate formulations releasing volatile organic compounds that are attractive for natural enemies of insect pests. However, the elaboration of slow-release devices that ensure stable and controlled release of active volatile compounds is quite challenging. Here, we developed a formulation based on E-β-farnesene and (–)-β-caryophyllene, these two semiochemicals having strong attractive potential on aphid natural enemies including ladybeetles and hoverflies. Both compounds were encapsulated together in alginate gel beads. The blend efficiency is currently being evaluated through laboratory and field assays. [less ▲]

Detailed reference viewed: 22 (3 ULg)
Peer Reviewed
See detailFirst evidence of a volatile sex pheromone in the invasive Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae)
Fassotte, Bérénice ULg; Fischer, Christophe ULg; Durieux, Delphine ULg et al

Conference (2014, April 02)

Data about sex pheromones, or any semiochemicals that play a role in Coccinellid mating, remain limited. Since years, various studies and behavioral observations have hypothesized that such molecules are ... [more ▼]

Data about sex pheromones, or any semiochemicals that play a role in Coccinellid mating, remain limited. Since years, various studies and behavioral observations have hypothesized that such molecules are involved in sexual communication of ladybeetles. In this study, we collected volatile organic compounds released by virgin females of the multicolored Asian ladybeetle, Harmonia axyridis (Pallas), which were either allowed or not allowed to feed on aphids. In the presence of aphids, virgin females exhibited “calling behavior”, which has been associated with the emission of a sex pheromone in several Coleoptera species. Bioassays showed that these females released a blend of volatile compounds that is involved in the attraction of males. Gas Chromatography-Mass Spectrometry analyses highlighted specific volatile cues emanating from females, whereas males did not produce these compounds. Five components were identified: (–)-β-caryophyllene, β elemene, methyl-eugenol, α humulene, and α bulnesene. All compounds were produced after virgin females were fed aphids, and their quantity increased across the experimental period. The results confirm that female H. axyridis produce a volatile sex pheromone. Therefore, this study provides important biological information that could promote the development of efficient pest control management methods to manipulate the movements of this invasive ladybeetle, and to reduce its negative impacts on biodiversity. [less ▲]

Detailed reference viewed: 24 (4 ULg)
Peer Reviewed
See detailStudy of sex ratio and morphotypes of the Multicoloured Asian Ladybird, Harmonia axyridis Pallas in Belgian corn
Vandereycken, Axel ULg; Fassotte, Bérénice ULg; Barsics, Fanny ULg et al

Poster (2014, April 02)

Several insect species are known to have different morphotypes, an adaptation to heterogeneities in changing environments. This phenotypic plasticity could be a factor used by the Multicoloured Asian ... [more ▼]

Several insect species are known to have different morphotypes, an adaptation to heterogeneities in changing environments. This phenotypic plasticity could be a factor used by the Multicoloured Asian Ladybird, which is considered as an invasive species in Europe since its introduction in the 80’s. Harmonia axyridis has four major morphotypes: 2 melanic forms, conspicua and spectabilis; and 2 non-melanic, succinea and axyridis. In Belgium, only the three first are observed. Literature reports variations in morphotype frequencies, across native and introduced strains. These variations seem to be linked to climate (geographical and seasonal variation), with non-melanic forms being the most abundant in hot and arid climate. This study focused on the variation of morphotype abundance in H. axyridis according to season, field and gender. Adult ladybirds were caught from mid-July to mid-October 2012 into 5 corn fields. The proportions of observed morphotypes were compared according to the sampling season. The abundance of morphotypes was not significantly different through the fields; the observed ratios were 81.6%, 15% and 3.4% for succinea, spectabilis and conspicua respectively. Succinea was the most observed morphotype (χ2=755.8; P<0.001). The total number of collected males and females was the same with ratios of 48.2% and 51.8%: (χ2=0.93; P=0.33). The sex ratio was also not significantly different for each morphotype. There was no evolution of melanic and non-melanic form ratio according to the season, with 25.6% of melanic form in summer and 20.6% in autumn (χ2=1.22; P=0.27). Our results seem to show that morphotype adaptation according to the climate is not observed for Belgian ladybirds. We discuss these results according to all environmental data available. [less ▲]

Detailed reference viewed: 15 (1 ULg)