Inverse soil parameter identification of an earth and rockfill dam by genetic algorithm optimization

in Sharing Experience for Safe and Sustainable Water Storage (2013)

Ion-Mobility mass spectrometry as a potential tool to assign disulfide bonds arrangements in peptides with multiple disulfide bridges.

in Analytical Chemistry (2013)

Disulfide bridges play a major role in defining the structural properties of peptides and proteins. However, the determination of the cysteine pairing is still challenging. Peptide sequences are usually ... [more ▼]

Disulfide bridges play a major role in defining the structural properties of peptides and proteins. However, the determination of the cysteine pairing is still challenging. Peptide sequences are usually achieved using MS/MS spectra of the totally reduced unfolded species but the cysteine pairing information is lost. On the other hand, MS/MS experiments performed on native folded species show complex spectra composed of non-classical ions. MS/MS alone does not allow the cysteine pairing nor the full sequence of an unknown peptide to be determined. The major goal of this work is to set up a strategy for the full structural characterization of peptides including disulfide bridges annotation in the sequence. This strategy was developed by combining Ion Mobility Spectrometry (IMS)and Collision Induced Dissociation(CID). It is assumed that the opening of one S-S bridges in a peptide leads to a structural evolution which results in a modification of IMS drift time. In the presence of multiple S-S bridges, the shift in arrival time will depend on which disulfide(s) has (have) been reduced and on the shape adopted by the generated species. Due to specific fragmentations observed for each species, CID experiments performed after the mobility separation could provide not only information on peptide sequence, but also on the localization of the disulfide bridges. To achieve this goal, synthetic peptides containing two disulfides were studied. The openings of the bridges were carried out following different experimental conditions such as reduction, reduction/alkylation or oxidation. Due to disulfide scrambling highlighted with the reduction approaches, oxidation of S-S bonds into cysteic acids appeared to be the best strategy. Cysteines connectivity was then unambiguously determined for the two peptides, without any disulfide scrambling interference. [less ▲]

L'organisation du pouvoir dans les groupes de sociétés

in Dal, Georges-Albert (Ed.) Droit des groupes de sociétés questions pratiques (2013)

La prévention des assuétudes : ce qu'en pensent les jeunes ? + DVD

Learning material (2013)

Impact of hemodynamic load on exercise capacity in aortic stenosis

in International Journal of Cardiology (2013)

EuroEcho and other imaging modalities: highlights

in European Journal of Echocardiography (2013)

Patterns of genomic aberrations suggest that Burkitt lymphomas with complex karyotype are distinct from other aggressive B-cell lymphomas with MYC rearrangement.

in Genes, Chromosomes & Cancer (2013), 52(1), 81-92

We previously showed that complex karyotypes (CK) and chromosome 13q abnormalities have an adverse prognostic impact in childhood Burkitt lymphomas/leukemias (BL) and diffuse large B-cell lymphomas (DLBCL ... [more ▼]

We previously showed that complex karyotypes (CK) and chromosome 13q abnormalities have an adverse prognostic impact in childhood Burkitt lymphomas/leukemias (BL) and diffuse large B-cell lymphomas (DLBCL). The aim of our study was to identify recurrent alterations associated with MYC rearrangements in aggressive B-cell lymphomas with CK. Multicolor fluorescence in situ hybridization (M-FISH) was performed in 84 patient samples (59 adults and 25 children), including 37 BL (13 lymphomas and 24 acute leukemias), 12 DLBCL, 28 B-cell lymphomas with intermediate features (DLBCL/BL), 4 B-cell precursor acute lymphoblastic leukemias (BCP-ALL), and 3 unclassifiable B-cell lymphomas. New (cytogenetically undetected) abnormalities were identified in 80% of patients. We also refined one-third of the chromosomal aberrations detected by karyotyping. M-FISH proved to be more useful in identifying chromosomal partners involved in unbalanced translocations and in revealing greater complexity of 13q rearrangements. Most of the newly identified or refined recurrent alterations involved 1q, 13q and 3q (gains/losses), 7q and 18q (gains), or 6q (losses), suggesting that these secondary aberrations may play a role in lymphomagenesis. Several patterns of genomic aberrations were identified: 1q gains in BL, trisomies 7 in DLBCL, and 18q-translocations in adult non-BL. BCP-ALL usually displayed an 18q21 rearrangement. BL karyotypes were less complex and aneuploid than those of other MYC-rearranged lymphomas. BCP-ALL and DLBCL/BL were associated with a higher rate of early death than BL and DLBCL. These findings support the categorization of DLBCL/BL as a distinct entity and suggest that BL with CK are indeed different from other aggressive MYC-rearranged lymphomas, which usually show greater genetic complexity. (c) 2012 Wiley Periodicals, Inc. [less ▲]

L'habitat-biblioteca di Francesco Appesi. Un confronto tra Il Miracolo e Tre Croci

in Intervalles (2013), 6

As Above, so Below: Narrative Salience and Side Effects of National Innovation Systems

in European Political Science (2013), accepté à publication

Evaluation of Electronic Nose Technology for Early-warning of Bio-methanation Process Disturbance

Doctoral thesis (2013)

An electronic nose (e-nose) is an instrument composed of an array of non specific gas sensors inspired by the biological olfactory system for the detection and recognition of odours. In this work, this ... [more ▼]

An electronic nose (e-nose) is an instrument composed of an array of non specific gas sensors inspired by the biological olfactory system for the detection and recognition of odours. In this work, this technology is evaluated to predict process disorders in agricultural anaerobic digestion reactors. Response of the sensor array is used to recognize a gas mixture by the use of multivariate data analysis techniques. In our case, an e-nose developed in our laboratory and composed of metal oxide semi-conductor gas sensors was equipped with a biogas dilution system to realize the measurements. When employed on small-scale digesters fed with simple feeding substrates (sucrose, lipids, mixture lipids and sucrose), the e-nose presented enthusiastic results for the detection of organic overload disorders. Multivariate process control charts were employed with the e-nose data to obtain a simple state indicator represented by the Hotelling's T² statistics. This indicator resumes the general state of the digesters when its value exceeds statistically defined control limit. Instability periods and recovery of the anaerobic digestion process when exposed to organic overfeeding could be thus precisely assessed. In addition, the use of a variety of substrates had no significant effect on the performance of the e-nose monitoring. Variations of methane content in biogas, as a result of use of substrates of different compositions, had neither any influence on the e-nose monitoring quality. Thereafter, an e-nose was placed online on continuously stirred tank reactor of 100 L over a period of 300 days. A multivariate monitoring method using recursive and adaptive principal component analysis (PCA) was applied to the e-nose data. This enabled to very efficiently compensate system drift and notably sensor drift. Moreover, adaptive PCA model could also adapt to environmental variations and to reactor slow shifts. T² statistics and its squared prediction error (SPE) obtained from the PCA model are very competitive state indicators compared to pH, alkalinity, gas production rate and methane and carbon dioxide content. Two electronic noses were also evaluated in real-scale agricultural anaerobic digestion plants. The low process state monitoring in both plants does not enable to conclude to the relevance of disorders observed by the e-nose apparatus and demonstrates the real need for online anaerobic digestion process state monitoring and its subsequent optimization in agricultural biogas plants. Even though, the performance of the recursive PCA monitoring is much superior to that of static PCA. Adaptive PCA proves to be a potential automatic drift and environmental variations correction method for online and real-time e-nose measures, notably for field applications. In conclusion, it was demonstrated in this work that e-nose technology has a real potential as an application of online monitoring of anaerobic digester state. Moreover, this research demonstrated that it was possible to realize a monitoring of the state of anaerobic digesters using only gas phase measurements. Another contribution is the use of multivariate process control techniques on e-nose data to obtain a simple state indicator as well as the use of adaptive PCA to automatically compensate drift and environmental effects on e-nose measurements. [less ▲]