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See detailAn Alternative to Semantics?
Gauvry, Charlotte ULg

Conference (2015, February 12)

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See detailNarration et jeu vidéo
Barnabé, Fanny ULg

Scientific conference (2015, February 12)

Cours sur la narration dans le jeu vidéo et sur la place qu'occupe cette problématique dans le champ des "game studies".

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See detailVolatile Organic Chemicals in the Rhizosphere of Barley, and their Role on the Foraging Behavior of Wireworms (Coleoptera: Elateridae)
Barsics, Fanny ULg

Doctoral thesis (2015)

Many species of wireworms, the larvae of click beetles (Coleoptera: Elateridae), are known as worldwide belowground pests of a large range of crops including cereals. Pesticide based agricultural ... [more ▼]

Many species of wireworms, the larvae of click beetles (Coleoptera: Elateridae), are known as worldwide belowground pests of a large range of crops including cereals. Pesticide based agricultural practices seem to have allowed significant population reduction in the past, but there is an increasing need for alternative control methods. In the first Chapter of this work, we review the current knowledge concerning 1) Integrated Pest Management (IPM) of wireworms and 2) their chemical ecology. These reviews shed light on promising approaches to develop new management strategies, as well as gaps of knowledge to be filled in that purpose. One of them is investigated here. The research aim of this PhD thesis (detailed in Chapter II) is the identification of the volatile organic compounds (VOC) released by the roots and used by wireworms to locate their food source. To achieve this goal, we defined three objectives: 1) developing a behavioral assay suitable for the study of wireworm orientation behavior under exposure to root-produced VOC; 2) profiling VOC released in the rhizosphere, according to different growth conditions; and 3) evaluating the role of the identified VOC on wireworm foraging behavior. Our experimental model involved Agriotes sordidus Illiger wireworms and Hordeum distichon L. (spring barley). In Chapter III, we report on the developmental steps of an adequate olfactometric method for wireworms. This includes an initial setup, the “Y-shaped” olfactometer, and dual-choice pipes gathering the necessary upgrades to efficiently observe the behavior of wireworms exposed to a VOC source. By resorting to live roots in a variety of features, we attempted to highlight appropriate abiotic and biotic experimental parameters, as well as the limits to the use of our olfactometric devices. The bioassays confirmed the attraction of wireworms towards VOC emitted by live roots. With a first sampling method on excised roots, Solid – Phase Micro – Extraction (SPME – semi-quantitative), coupled to Gas Chromatography – Mass Spectrometry (GC-MS), we detected a high number of molecules that might act as semiochemicals on wireworms, notably in our bioassays. In the fourth Chapter, the bioassays were performed with selected VOC, detected during the chemical profiling. Firstly, we assessed if wireworms were attracted towards 2 pentylfuran, the main VOC highlighted with SPME. The molecule was submitted to the larvae by encapsulation in alginate beads. An adequate formulation allowed reaching an emission rate matching quantification estimates. In the aim to improve the VOC profiling, we also used Dynamic Head-Space Sampling (DHS), on roots ground in liquid nitrogen. It highlighted four volatile aldehydes. Their biological activity on wireworms was evaluated thanks to glass wool/triacetin-based dispensers, inserted in the dual-choice pipes. Through this work, we show that VOC produced by barley roots carry information useable by foraging wireworms. The two sampling methods indicate two different modalities of root-VOC production and are encouraging for further understanding of belowground VOC production and emission. The developed olfactometric method and related results open new perspectives to increase knowledge on wireworm ecology. Combined to other developments in research on their management, they could lead to interesting, innovative and ecological management practices. [less ▲]

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See detailQuand la clinique bouscule les concepts
Englebert, Jérôme ULg

Scientific conference (2015, February 11)

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See detailRisk of emergence of a hyperpathogenic bovine leukemia virus by mutation of a single envelope N-linked glycosylation site
De Brogniez, Alix ULg; Bouzar, Amel-Baya; Jacques, Jean-Rock ULg et al

Poster (2015, February 11)

- Introduction : Pathogens have co-evolved with their host to ensure efficient replication and transmission without inducing excessive pathogenicity that would indirectly impair their persistence. This is ... [more ▼]

- Introduction : Pathogens have co-evolved with their host to ensure efficient replication and transmission without inducing excessive pathogenicity that would indirectly impair their persistence. This is exemplified by the bovine leukemia virus (BLV) system in which lymphoproliferative disorders develop in ruminants after latency periods of several years. Infection of sheep and cattle with BLV is a model system for the related human T-lymphotropic virus type 1 (HTLV-1) responsible for Adult T-cell Leukemia (ATL). - Aims : The goal of this work is to investigate the role of N-glycans of the viral envelope protein during viral replication and pathogenesis. - Methods and results : Using glycosylation inhibitors and lectins, we showed that N-glycosylation is involved in viral infection (i.e. cell-to-cell fusion). Using reverse genetics of an infectious molecular provirus, we next demonstrated that a particular N-linked envelope glycosylation site (N230) limits viral replication and pathogenicity in vitro and in vivo. We have thus generated a viral mutant that is more pathogenic than the wild type strain. - Conclusions : To our knowledge, this is the first time that a hyperpathogenic BLV has been identified. This unexpected observation has important consequences in terms of disease control and managing. Indeed, during evolution, pathogens and their hosts should achieve an equilibrium allowing the coexistence of the two species. Occurrence of this particular mutation may thus represent a potential threat associated with emergence of hyperpathogenic BLV strains and possibly of new variants of the related HTLV-1. [less ▲]

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See detailMener pratiquement ses entretiens: retour d'expériences
Parotte, Céline ULg

Learning material (2015)

Trucs et astuces pour mener pratiquement ses entretiens qualitatifs. Pratiquement, cette présentation a pour objectif de mettre l'étudiant en situation concrète: que faire? à quoi penser? comment me ... [more ▼]

Trucs et astuces pour mener pratiquement ses entretiens qualitatifs. Pratiquement, cette présentation a pour objectif de mettre l'étudiant en situation concrète: que faire? à quoi penser? comment me préparer? [less ▲]

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See detailCheckpoints modulation by the human T-lymphotropic virus type 1 (HTLV-1) Tax protein : towards new therapeutic approaches
Carpentier, Alexandre ULg; Barez, Pierre-Yves ULg; Boxus, Mathieu et al

Poster (2015, February 11)

HTLV-1 infects approximately 20 million people worldwide and causes several diseases. This virus is responsible for the adult T-cell leukemia (ATL) and for a chronic neuropathology (TSP/HAM). There is ... [more ▼]

HTLV-1 infects approximately 20 million people worldwide and causes several diseases. This virus is responsible for the adult T-cell leukemia (ATL) and for a chronic neuropathology (TSP/HAM). There is currently no satisfactory treatment for these diseases. Among the proteins encoded by HTLV-1, Tax appears to play an important role in the mechanisms leading to pathogenicity. We are interested in the mechanisms of cell transformation by HTLV-1 and more particularly in the interplay between the viral Tax oncoprotein and the DNA damage response (DDR). We demonstrate that transient expression of Tax results in DNA damage, cell cycle arrest and activation of the ATM-Chk2-p53 axis of the DDR. In fibroblasts, cell cycle arrest occurs at the G1 and G2 phases depending on the p53 background. Despite Tax expression hampers cell cycle progression, neither pro-apoptotic nor pro-senescent effects are observed. In contrast, HTLV-1 infected lymphocytes proliferate continuously and appear to be adapted to the checkpoints. This mechanism allows infected lymphocytes to proliferate despite the presence of genomic lesions. Those cells might thus rely on effective DNA repair mechanisms. Indeed, we show that Tax expressing cells activate the error free repair mechanism homologous recombination (HR). Inhibition of ATM, involved in DDR and DNA repair by HR, impedes Tax-mediated cellular transformation. Depending on these observations, we propose a novel therapeutic approach based on the principle of synthetic lethality. [less ▲]

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See detailReprogramming of replication origin firing and checkpoint adaptation in adult T-cell leukemia
Carpentier, Alexandre ULg; Barez, Pierre-Yves ULg; Boxus, Mathieu et al

Conference (2015, February 11)

Human T-lymphotropic virus type 1 (HTLV-1) is a retrovirus that infects about twenty million individuals worldwide. HTLV-1 is the causative agent of different diseases among which the most common are the ... [more ▼]

Human T-lymphotropic virus type 1 (HTLV-1) is a retrovirus that infects about twenty million individuals worldwide. HTLV-1 is the causative agent of different diseases among which the most common are the adult T-cell leukemia (ATL) and a neurodegenerative disorder called HAM/TSP (Human associated myelopathy/ Tropical spastic paraparesis). A key parameter of HTLV-1 pathogenesis is faster replication of provirus-carrying lymphocytes allowing clonal expansion of infected cell populations. The virally-encoded Tax oncoprotein plays an essential role in this process by interacting with DNA replication origins and accelerating S phase progression. By reprogramming the timing of origin firing, Tax also creates a replicative stress leading to DNA double strand breaks. This mechanism further triggers the DNA damage response (DDR) that induces cell cycle arrest and initiates either apoptosis or senescence. However, HTLV-1 infected cells have developed strategies to interfere with the DDR and are adapted to checkpoint control. These cells are thus able to proliferate despite occurrence of DNA damage. Based on these observations, we now propose a novel therapeutic approach based on the principle of synthetic lethality. [less ▲]

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See detailEnvironmental / climatic changes & the people ...
Ozer, Pierre ULg

Scientific conference (2015, February 11)

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See detail“Problem Practices” in EU Competition Law
Petit, Nicolas ULg

Scientific conference (2015, February 10)

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See detailC'est la faute à l'Europe !
Grandjean, Geoffrey ULg

Conference given outside the academic context (2015)

Nombreux sont les dirigeants politiques et les citoyens qui considèrent que l’Union européenne est responsable de la situation politique et économique actuelle. L’exposé vise à interroger ces discours et ... [more ▼]

Nombreux sont les dirigeants politiques et les citoyens qui considèrent que l’Union européenne est responsable de la situation politique et économique actuelle. L’exposé vise à interroger ces discours et à comprendre la place et le rôle des institutions européennes. Il permet surtout de relativiser ces discours en ciblant précisément les marges de manœuvre de l’Union européenne. [less ▲]

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See detailCognitive remediation of autobiographical memory in schizophrenia and its relation with depressive mood.
Blairy, Sylvie ULg

Scientific conference (2015, February 09)

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See detailCritique des données statistiques 'macro' disponibles dans les institutions internationales
Ozer, Pierre ULg

Scientific conference (2015, February 09)

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See detailSome misunderstood or unknown LaTeX tricks (XVI)
Merciadri, Luca ULg

E-print/Working paper (2015)

This is the second (and last) part of the tricks I found on StackExchange. First part began in the preceding issue. We will begin by a personal trick, and end by some inquiry about my LaTeX Tips & Tricks.

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See detailSome misunderstood or unknown LaTeX tricks (XV)
Merciadri, Luca ULg

E-print/Working paper (2015)

After having looked at about 150 StackExchange pages of 50 topics (that sums up to about 7500 topics’ names), I give you, in this article and in the next issue, the most interesting topics’ answers to my ... [more ▼]

After having looked at about 150 StackExchange pages of 50 topics (that sums up to about 7500 topics’ names), I give you, in this article and in the next issue, the most interesting topics’ answers to my eyes. We will first begin by two additions in regard to 31(3), 32(1). [less ▲]

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Peer Reviewed
See detailSynthesis and anticancer activities of a novel class of mono- and di-metallic Pt(II)(salicylaldiminato)- (DMSO or Picolino)Cl complexes
Faiz-Ur Rahman; Ali, Amjad ULg; Rong Guo et al

in Dalton Transactions (2015)

A series of novel Pt(II) complexes [cis- and trans-Pt(II)(salicylaldimine)(DMSO)Cl (C-1), trans-Pt(II)(salicylaldimine)( 4-picoline)Cl (C-2), Pt(II)(salicylaldimine)Cl (C-3), trans- and cis/trans-Pt2(II ... [more ▼]

A series of novel Pt(II) complexes [cis- and trans-Pt(II)(salicylaldimine)(DMSO)Cl (C-1), trans-Pt(II)(salicylaldimine)( 4-picoline)Cl (C-2), Pt(II)(salicylaldimine)Cl (C-3), trans- and cis/trans-Pt2(II)(salicylaldimine) (DMSO)2Cl2 (C-4), trans-Pt2(II)(salicylaldimine)(4-picoline)2Cl2 (C-5) was synthesized and characterized. The structures of C-1-cis, C-1-trans and C-3 were determined using a single crystal X-ray analysis. This class of Pt(II) complexes has been studied for their in vitro cytotoxicity in multiple human cancer cell lines, including breast (MCF-7), liver (HepG2), lung (A549), colon (HCT116) and cervical (Hela) cancers. C-1- trans, C-2 and C-4-trans showed significant cytotoxicity to these cancer cells comparable to cisplatin. A time- and dose-dependent MTT assay revealed that these complexes can suppress cell viability and cell proliferation. Mechanistically these complexes induced pro-apoptotic gene expression such as BAX, PUMA and NOXA and thereby enhanced apoptosis. Moreover, PARP cleavage in a dose-dependent manner indicated their cytotoxic effect against cancer cells. Apoptosis of cancer cells occurred through apoptotic pathways as explained by the cytometry analysis. The DNA unwinding properties of these active Pt(II) complexes were studied by gel electrophoresis using pBR322 plasmid DNA as a target. Changes in the morphology of cancer cells were also observed upon the addition of C-1-trans, C-2 and C-4-trans. [less ▲]

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See detailZones humides : entre modifications environnementales et actions humaines
Ozer, Pierre ULg

Conference (2015, February 08)

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See detailMethionine-derived Schiff base as selective fluorescent “turn-on”chemosensor for Zn2+in aqueous medium and its application in livingcells imaging
Faiz-Ur-Rahman; Ali, Amjad ULg; Rong Guo et al

in Sensors and Actuators B: Chemical (2015)

tWe describe the quantitative synthesis of a Schiff base derived from methionine and salicylaldehyde(SMSB) which displayed turn-on selective fluorescent chemo-sensing properties for Zn2+in bufferedCH3CN ... [more ▼]

tWe describe the quantitative synthesis of a Schiff base derived from methionine and salicylaldehyde(SMSB) which displayed turn-on selective fluorescent chemo-sensing properties for Zn2+in bufferedCH3CN/H2O (1:1) solution. In physiological pH (7.2 buffered) SMSB exhibited high selectivity and sen-sitivity toward Zn2+over the other selected metal ions. Upon the addition of Zn2+33 folds of emissionenhancement was observed in SMSB and limit of detection (CL) for Zn2+was found to be 5.0 × 10−7M. Thisbinding phenomenon was studied by1H NMR and HR-MS analysis. Mass spectrometry analysis revealedthat SMSB interacts with Zn2+and form a 1:1 complex, also confirmed by Job’s plot. This probe wascompletely retrieved by adding EDTANa2solution, exhibited a fluorescent quenching effect by removingZn2+from the sensor. SMSB was observed to have a good tolerance for fluorescence detection of Zn2+in pH range 6–10. Furthermore it is successfully investigated for living cells imaging using MCF-7 cells.Combine experimental results indicated SMSB to be a useful and powerful chemosensing probe for Zn2+in water as well as in living cells. [less ▲]

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See detailQuand science politique et droit dialoguent : quelle typologie des instruments mémoriels en fonction du degré de contrainte ?
Grandjean, Geoffrey ULg

Conference (2015, February 07)

Envisager les études sur la mémoire comme sous-champ de la science politique francophone ouvre des pistes enrichissantes pour les politologues, notamment lorsqu’ils sont amenés à analyser les instruments ... [more ▼]

Envisager les études sur la mémoire comme sous-champ de la science politique francophone ouvre des pistes enrichissantes pour les politologues, notamment lorsqu’ils sont amenés à analyser les instruments mémoriels régissant un ou plusieurs système(s) politique(s). Dans cette perspective, la communication vise à présenter, d’un point de vue épistémologique, les apports découlant de l’interaction entre les sciences politique et juridique dans le processus de consolidation des études sur la mémoire comme sous-champ disciplinaire de la science politique. Plus précisément, la communication vise à offrir une typologie des instruments mémoriels en fonction du degré de contrainte et d’imposition d’une mémoire officielle. Par exemple, une résolution adoptée par une assemblée législative est faiblement contraignante alors qu’une loi réprimant le négationnisme impose à la fois une mémoire officielle tout en sanctionnant pénalement les citoyens qui ne la respectent pas. La typologie passe en revue les multiples instruments mémoriels pouvant être adoptés par les autorités publiques : résolution, loi déclarative, loi pénale, plan de commémorations ou encore mise en valeur d’un lieu de mémoire. L’accent est mis sur les instruments mémoriels de deux pays francophones : la Belgique et la France. Au final, cette communication permet d’insister sur l’apport de la science politique en analysant l’exercice de prérogatives de puissance publique et sur l’apport de la science juridique en classifiant les types d’instruments mémoriels en fonction du degré de contrainte ; tout en faisant dialoguer des cas d’étude différents. [less ▲]

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