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See detailGenetic evaluation for birth and conformation traits in dual-purpose Belgian Blue cattle using a mixed inheritance model
REIS MOTA, Rodrigo ULiege; Mayeres, P.; Bastin, Catherine et al

in Journal of Animal Science (2017)

The segregation of the causal mutation (mh) in the muscular hypertrophy gene in dual-purpose Belgian Blue (dpBB) cattle is considered to result in greater calving difficulty (dystocia). Establishing ... [more ▼]

The segregation of the causal mutation (mh) in the muscular hypertrophy gene in dual-purpose Belgian Blue (dpBB) cattle is considered to result in greater calving difficulty (dystocia). Establishing adapted genetic evaluations might overcome this situation through efficient selection. However, the heterogeneity of dpBB populations at the mh locus implies separating the major gene and other polygenic effects in complex modeling. The use of mixed inheritance models may be an interesting option because they simultaneously assume both influences. A genetic evaluation in dpBB based on a mixed inheritance model was developed for birth and conformation traits: gestation length (GL), calving difficulty (CD), birth weight (BiW), and body conformation score (BC). A total of 27,362 animals having records were used for analyses. The total number of animals in the pedigree used to build the numerator relationship matrix was 62,617. Genotypes at the mh locus were available for 2,671 animals. Missing records at this locus were replaced with genotype probabilities. A total of 13,221 (48.3%) were registered as dpBB, 1,287 (4.7%) as beef Belgian Blue, and 12,854 (47.0%) were unknown. From those 13,221dpBB animals, 650, 849, and 534 had double or single copies or no copy, respectively, of the causal mutation (mh) in the muscular hypertrophy gene, whereas 11,188 had missing genotypes. This heterogeneity at the mh locus may be the reason for high variability in the studied traits, that is, high heritability estimates of 0.33, 0.30, 0.38, and 0.43 for GL, CD, BiW, and BC, respectively. In general, additive (P < 0.05) and dominance (P < 0.001) allele substitution for calves and dams had significant impact for all traits. The moderate coefficient of genetic variation (27.80%) and high direct heritability (0.28) for CD suggested genetic variability in dpBB and possible genetic improvement through selection. This variability has allowed dpBB breeders to successfully apply mass selection in the past. Genetic trend means from 1988 to 2016 showed that sire selection for CD within genotype was progressively applied by breeders. The selection intensity was more important for CD in double-muscled lines than in segregated lines. Our study illustrated the possible confusion caused by the use of major genes in selection and the importance of fitting appropriate models such as mixed inheritance models that combine polygenic and gene content information. [less ▲]

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See detailQuantification of Temperature with Time-lapse Electrical Resistivity Using the Prediction-focused Approach -A Field Case
Hermans, Thomas ULiege; Nguyen, Frédéric ULiege; Caers, Jef

in EarthDoc (2017, September 06)

Standard inversion of time-lapse geophysical suffers from spatially and temporally varying resolution due to the regularization procedure used during the inversion process. In this study, we apply the ... [more ▼]

Standard inversion of time-lapse geophysical suffers from spatially and temporally varying resolution due to the regularization procedure used during the inversion process. In this study, we apply the recently developed prediction-focused approach (PFA) to directly estimate temperature with electrical resistance data, without classic tomographic inversions. PFA is based on a set of prior subsurface models coherent with our prior knowledge of the site. From this set of models, we generate a prior set of temperature distribution and resistance data mimicking the field experiment. Then, we use dimension-reduction techniques to derive a direct relationship between the data and the desired prediction. The use of canonical correlation analysis linearize the relationship and allows using Gaussian regression to sample the posterior. In this paper, we demonstrate the ability of PFA to process time-lapse ERT data during a field experiment. We propose an analysis of time-lapse reciprocals to derive an error model and generate the posterior distribution of temperature. We validate the results using direct measurements in the aquifer. This successful application opens new ways to process and integrate geophysical data in hydrogeological model. [less ▲]

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See detailSpatio-temporal Monitoring of Heat Storage in a Shallow Aquifer Using Electrical Resistivity 4D Imagery and DTS
Lesparre, Nolwenn ULiege; Hermans, Thomas ULiege; Nguyen, Frédéric ULiege et al

in EarthDoc (2017, September 06)

The design of groundwater heat pumps requires a good understanding of the aquifer and heat flow conditions. Issues of short-circuit or recycling between cold and hot wells have to be carefully considered ... [more ▼]

The design of groundwater heat pumps requires a good understanding of the aquifer and heat flow conditions. Issues of short-circuit or recycling between cold and hot wells have to be carefully considered. Surface geophysical methods allow monitoring subsurface processes without additional perturbations of the medium. Within available methods, the electrical resistivity imagery (ERI) applied in time-lapse (TL) is appropriate. Here, we monitored with ERI and distributed temperature sensors (DTS) a heat plume propagation during an experiment of hot water injection in a shallow aquifer. DTS and TL ERI measurements acquired from two boreholes provide a local estimate of the heat propagation through the medium. TL ERI were also performed from a grid at surface to follow the 3D plume shape formation and evolution through time. The different complementary data validate the potential of surface TL ERI for monitoring in 3D the behavior of shallow heat plumes. ERI highlight the heterogeneity of the aquifer by distinguishing regions with higher or lower hydraulic conductivity. In the higher hydraulic conductivity zone, the heat might be evacuated through water flow, while in the lower hydraulic conductivity area heat storage is achievable. Thus, in that last region the plume temperature decreases progressively with time. [less ▲]

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See detailRapport préliminaire en vue d'une soutenance de thèse
Swennen, Philippe ULiege

Report (2017)

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See detailOptimization and machine learning for smart-microgrids
Cornélusse, Bertrand ULiege

Conference (2017, September 06)

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See detailIdentification, Characterization and Full-Length Sequence Analysis of a Novel Polerovirus Associated with Wheat Leaf Yellowing Disease
Zhang, Peipei; Liu, Yan; Liu, Wenwen et al

in Frontiers in Microbiology (2017), 8(1689),

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See detailGoing Inside Structural and Physicochemical Properties of Polymers using Ion Mobility-Mass Spectrometry
Haler, Jean ULiege; de la Rosa, Victor R.; Far, Johann ULiege et al

Conference (2017, September 06)

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See detailUsing Geophysical Hard Data to Enhance the Reliability of Hydrological Models
De Schepper, Guillaume; Paulus, Claire; Molron, J. et al

in EarthDoc (2017, September 06)

Appropriate design of geophysical experiments combined with common hydrological measurements offer opportunities to use geophysical data as hard data in hydrological models, regarding their ... [more ▼]

Appropriate design of geophysical experiments combined with common hydrological measurements offer opportunities to use geophysical data as hard data in hydrological models, regarding their conceptualisation or their calibration. Two study sites located in Wallonia, Belgium, were investigated. In the first case (fractured limestone aquifer), streaming potentials were linked to piezometric measurements, allowing us to better conceptualise the local groundwater flow model and calibrate it. In the second example (alluvial sandy aquifer), the use of 4D electrical resistivity tomography and temperature measurements appeared to be a reliable methodology to predict heat storage and recovery cycles in hydrological models with a better constrained calibration process. [less ▲]

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See detailEnvironmental impact of food waste treatment in an EcoCleaner, a portable accelerated composter
Groslambert, Sylvie ULiege; Léonard, Angélique ULiege; Finet, Sébastien

Poster (2017, September 06)

Canteen and catering food wastes represent a challenge in waste management since they contain a large part of water and can be subject to biological processes during the waste management chain, especially ... [more ▼]

Canteen and catering food wastes represent a challenge in waste management since they contain a large part of water and can be subject to biological processes during the waste management chain, especially during storage. Different solutions exist to manage this organic waste, for instance local and centralized composting, anaerobic digestion, or co-incineration with municipal waste. Except for local composting, theses methods need to collect and transport food waste to be processed, which mean transporting a lot of water. The Life Cycle in Practice (LCiP) (LIFE12 ENV/FR/001113) project helps SMEs to reduce the environmental impacts of their products and services across the entire life cycle. Within the frame of this project, the environmental impact of the Eco-Cleaner system is evaluated with the standardized life cycle assessment (LCA) methodology. Eco-Cleaner (EC) is a stand alone accelerated system that can transform food waste in valuable compost in only 24 hours. It is designed by Biowaste Recycling (http://biowasterecycling.com/). It can be declined in different capacities, from family size (about 1-2 kg/days of food waste) to large communities of 1300 place settings (up to 700 kg/days). EC technology is based on a permanent control of the ratio of humidity and temperature to optimize fermentation rate of food waste by a special consortium of more than thirty thermophilic bacteria. Waste reduction is about 90% and compost is sanitized to ensure the destruction of active parasites, insect eggs and germs. EC compost is dry (80-90% siccity level), and it has a quite high nitrogen content (about 3-4% on dry matter) and is rich in organic matter. Thanks to an activated carbon filter (replaced every two years), it is odourless and has no noxious gas emission. Since it's dry, this compost can be stored without further degradation, and its transport has a reduced environmental cost. The functional unit is the composting of one ton of food waste. The inventory includes the machine itself, its use, its refitting every twelve years, and its end of life. Refurbishing includes mainly the replacement of the motor, the heating resistance, the plastic parts, the seals, and only 5% of metals. Avoided chemical fertilizers consecutive to compost application are considered as system expansion, and it induces negative impact values in all categories. Electricity for use is the main impacting element in all categories (BE mix) but this impact can be reduced by the use of on-site photovoltaic panels. Abiotic depletion is highly impacted due to the metals the EC is made of (92% of total weight, principally steel), but a large improvement is achieved by the quadruplicating of the life span because of refurbishing, and the recycling of metals at the end of life. Specific process data are processed in SimaPro 8.1 software, using Ecoinvent 3.1 database for additional generic data, and analysed with the CML IA 3.03 method. [less ▲]

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See detailEtude de la résilience des forêts aménagées par l'approche des services écosystémiques
Maebe, Laura ULiege

Scientific conference (2017, September 06)

Detailed reference viewed: 23 (3 ULiège)
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See detailAnalysis of definitions of General Practice/Family Medicine and Primary Health Care
Jamoulle, Marc ULiege; Resnick, Melissa; Ittoo, Ashwin ULiege et al

in British Journal of General Practice (2017), 0050

Abstract Background There are numerous definitions of General Practice and Family Medicine (GP/FM) and Primary Health Care (PHC), but the distinction between the two concepts is unclear. Aim To conduct a ... [more ▼]

Abstract Background There are numerous definitions of General Practice and Family Medicine (GP/FM) and Primary Health Care (PHC), but the distinction between the two concepts is unclear. Aim To conduct a terminological analysis of a set of definitions of GP/FM and of PHC, to clarify what binds and what distinguishes these two concepts. Design The terms of 20 definitions were collected in two bags of words (one for GP/FM and one for PHC terms). A terminological analysis of these two bags of words was performed to prioritize the terms and analyze their world of reference. Methods The two collected bags of words were extracted with Vocabgrabber®, configured in two term butts using Wordle®, and further explored for similarities using Tropes®. The prioritized terms were analyzed using the Aristotelian approach to categorization of things. Results Although continuity of care (with person-centered approach and shared decision making) is the central issue of the two sets, the two sets of definitions differ greatly in content. The prioritized terms specific to GP/FM (community, medicine, responsibility, individual, problem, needs, ...) are different from prioritized terms specific to PHC (home, team, promotion, collaborator, engagement, neighborhood, medical center…). Conclusion Terminological analysis of the definitions for GP/FM and PHC shows two entities which are overlapping but distinct, necessitating a different taxonomic approach and different bibliographic search strategies. [less ▲]

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See detailProtein-polysaccharide complexes, a tool for protein delivery in CaCO3 microparticles
Ramalapa, Bathabile ULiege; Crasson, Oscar ULiege; Vandevenne, Marylène ULiege et al

Poster (2017, September 06)

INTRODUCTION The controlled delivery of proteins within calcium carbonate (CaCO3) particles is currently widely investigated. The success of these carriers has been driven by the ionic interactions ... [more ▼]

INTRODUCTION The controlled delivery of proteins within calcium carbonate (CaCO3) particles is currently widely investigated. The success of these carriers has been driven by the ionic interactions between proteins and particles making the encapsulation of proteins highly dependent on the pH of reaction solutions and the isoelectric point of the protein.1 This poses a great limitation on the successful loading of proteins into microparticles. In this study, we explored the use of polysaccharide-protein interactions to strongly enhance the encapsulation of proteins in CaCO3 microparticles. EXPERIMENTAL METHODS Previously, Vandevenne and colleagues2 inserted a human chitin binding domain (ChBD) that has intrinsic affinity for hyaluronic acid (HA) into β-lactamase (BlaP). This generated chimeric protein, named BlaPChBD, was shown to be fully bifunctional. In this study this hybrid protein (BlapChBD) was associated to HA and successfully loaded into CaCO3 microparticles using super critical CO2 technology aided by the templating effect of HA on CaCO3. Furthermore, thrombin cleavage sites were engineered on both sides of the inserted ChBD in the chimeric BlaP so that release of the protein from the microparticles could be easily achieved by protease cleavage. The microparticles were characterised for size, surface charge, poly-morphism and protein loading and in-vitro release assays were performed. RESULTS AND DISCUSSION The presence of ChBD inserted into the β-lactamase increased the encapsulation of the protein by 6-fold when complexed with HA (Fig. 1). In addition, we also showed that the encapsulated BlaP remains stable during this process using kinetic reaction of β-lactam hydrolysis. Our data showed that vaterite CaCO3 microparticles of sizes ranging between 4 and 7 µm were produced. We were also able to demonstrate that thrombin cleavage increased the release of the protein from the microparticles within 36 hours from <25% to 87% (Fig. 2). In conclusion, the presence of ChBD successfully improved the encapsulation yield of the protein while retaining up to 81% of its activity. CONCLUSION Protein-polysaccharide complexation demonstrates an excellent approach for the delivery of sensitive biomacromolecules which can otherwise be complicated due to electrostatic hindrances. Future prospects include using the methods we have developed for encapsulation of therapeutic proteins and using calcium carbonate as a carrier and scaffold in bone regeneration for example. [less ▲]

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See detailComparison of the electrochemical performance of Li4Ti5O12 spinel as negative electrodes for lithium-ion batteries prepared by sol gel and spray drying methods
Mahmoud, Abdelfattah ULiege; Piffet, Caroline ULiege; Berardo, Loris ULiege et al

Poster (2017, September 05)

Energy is considered as the lifeblood of modern society. Rechargeable batteries are the most promising to meet the human needs concerning the energy storage thanks their high energy density and high ... [more ▼]

Energy is considered as the lifeblood of modern society. Rechargeable batteries are the most promising to meet the human needs concerning the energy storage thanks their high energy density and high energy efficiency. Most difficult challenges of the development of promising rechargeable batteries concern the electrode materials. Li4Ti5O12 (LTO) is one the most promising anode materials for Li-ion batteries, as it demonstrates very stable cycling stability and excellent safety. Its high operating potential (~1.5 V) allows to avoid the formation of SEI during the first cycle. The three-dimensional structure offers LTO excellent reversibility due to the near zero volume strain during the Li+ ion intercalation and deintercalation cycling. The main objective of this study on LTO samples was to evidence the effect of synthesis method and thermal conditions on their structural, morphological and electrochemical properties [1, 2]. The results demonstrate the strong influence of the synthesis route (Sol-Gel and spray-drying methods) and the thermal treatment on the capacity, cyclability and rate capability of the LTO spinel in Li-half-cell and Li-ion full-cell (see Figure 1). References [1] A. Mahmoud, J. M. Amarilla, K. Lasri, I. Saadoune, Electrochim. Acta 93 (2013) 163-172. [2] A. Mahmoud, J. M. Amarilla, I. Saadoune, Electrochim. Acta 163 (2015) 213-222. [less ▲]

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See detailOrdovician to Early Devonian from South America: a state of the art - review
Rubinstein, C.V.; Garcia Muro, V.J.; Gerrienne, Philippe ULiege et al

Conference (2017, September 05)

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See detailétude fonctionnelle des facteurs d’épissage SR (Ser/Arg-rich) au cours du développement embryonnaire de Danio rerio.
Joris, Marine ULiege

Doctoral thesis (2017)

Ser/Arg-rich (SR) proteins constitute a phylogenetically conserved family of RNA binding proteins characterized by one or two RNA-recognition motifs (RRMs) at the N-terminus and a C-terminal domain ... [more ▼]

Ser/Arg-rich (SR) proteins constitute a phylogenetically conserved family of RNA binding proteins characterized by one or two RNA-recognition motifs (RRMs) at the N-terminus and a C-terminal domain enriched in Ser/Arg dipeptides. They are important regulators of constitutive and alternative splicing. Moreover, SR proteins are involved in many other aspects of RNA metabolism, including transcription, non-sense mediated decay, mRNA export, translational control as well as maintenance of genome stability. While the roles of SR splicing factors have been widely studied at a molecular level, their functions during animal cell differentiation and development are still largely undetermined. In this study, we decided to take advantage of the vertebrate model organism Danio rerio (zebrafish) to investigate SR protein functions by using molecular and genetic approaches. Fifteen zebrafish SR genes were identified belonging to the three subfamilies of SR proteins [SRSF1 (1RRM and 1 pseudo-RRM), SRSF2 (1RRM) and SRSF7 (1RRM +1ZnK)] initially described in humans. The current PhD thesis aimed to study of four of them, Srsfs5a, Srsf9, Srsfs7 and Srsf2b. We first determined the expression pattern of all SR genes by in situ hybridization at 24, 48 and 72 hours post-fertilization. This analysis revealed an ubiquitous expression pattern for SR genes, however pointing a higher expression in the brain and in the eyes. We next performed “knock-down” experiments using morpholinos (MOs) microinjection to determine SR gene functions. One splicing MO, designed to target srsf5a (sMOsrsf5a) showed an interesting phenotype that coincides with the expression pattern of the gene in the eyes. As none of the four other MOs used to target srsf5a could confirm the phenotype, we generated stable knockout mutant lines using TALEN and CRISPR/Cas9. Interestingly, the corresponding homozygous mutants did not display any phenotypic traits. These inconsistencies were attributed to a possible genetic compensation mechanism in mutants, but also to an underestimated large number of inadvertent morpholino RNA targets. Indeed, we uncovered that only 11 consecutive bases complementary to the 25 MO bases are sufficient for binding and subsequent blocking of splice sites, suggesting that the sMOsrsf5a induced phenotype was not due to the inactivation of this unique, specific gene. In addition, we observed that sMOsrsf5a secondary targets can be slightly reduced by increasing embryos growth temperature after microinjection. Our study contributes to the debate concerning MO specificity, efficacy and the number of unknown targeted sequences.   [less ▲]

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See detailThe Cyanobacterial Biota of Polar Regions: a Molecular Approach
Stelmach Pessi, Igor ULiege

Doctoral thesis (2017)

Cyanobacteria are major components of Polar ecosystems. They comprise a considerable amount of the total biomass, are the main primary producers, and play important roles in the initial development of ... [more ▼]

Cyanobacteria are major components of Polar ecosystems. They comprise a considerable amount of the total biomass, are the main primary producers, and play important roles in the initial development of terrestrial and aquatic habitats. Although the cyanobacterial diversity in the Polar Regions has been well studied, research has been mostly limited to traditional methods such as microscopy, culturing, and Sanger-based molecular ecology approaches. High-troughput sequencing (HTS) has revolutionized our knowledge of microbial diversity and distribution, but a thorough investigation of polar cyanobacterial communities using HTS has not yet been performed. As a result, knowledge of the distributional patterns of polar cyanobacterial communities and the mechanisms underlying their structure is fragmentary. In this thesis, we applied the HTS approach to investigate the diversity and distributional patterns of cyanobacteria in a range of polar habitats. The main goals were: 1. To evaluate the use of HTS as a tool for assessing the cyanobacterial diversity in polar environments, including an assessment of different bioinformatics pipelines and the improvement represented by HTS in comparison to traditional methods; 2. To assess the spatial patterns of cyanobacterial communities across polar environments and the environmental factors shaping cyanobacterial community composition; 3. Tho study the successional dynamics of cyanobacterial communities following the colonization of recently deglaciated habitats. Due to its higher analytical depth, HTS was proven a valuable tool for the study of the cyanobacterial biota of Polar Regions. Nevertheless, the analysis of artificial communities highlighted the importance of strict bioinformatics control of HTS reads, as PCR and sequencing errors might bias the observed structure of the communities. Given the strict bioinformatics workflow applied, our results suggest a previously undetected polar cyanobacterial rare biosphere. On one side, the HTS approach revealed that microbial mat and biological soil crust (BSCs) across different Antarctic and Svalbard (High Arctic) regions are somewhat homogeneous, being dominated by filamentous cyanobacteria from the orders Pseudanabaenales and Oscillatoriales. At the phylotype level (97.5% similarity threshold), on the other hand, cyanobacterial communities were highly variable along environmental gradients within and between habitats. A study of benthic mat communities in Antarctic lakes revealed cyanobacterial communities highly structured by lake conductivity, and comparable compositions in lakes from distant Antarctic regions. In a study of BSC communities in four nunataks and mountain ridges in the Sør Rondane Mountains (Dronning Maud Land), highly dissimilar communities were observed between locations. Finally, two studies of BSC communities in Petunia Bay (Svalbard) revealed a marked, linear cyanobacterial community turnover along gradients of soil development. Altogether, the results obtained in the present thesis show that HTS is a powerful yet underused tool for the study of cyanobacterial diversity, not only in Polar Regions, but also on a global scale. This can be attributed to the very high sequencing depth achieved by HTS, which provides more robust assessments of community turnover and with a stronger statistical support than Sanger- based molecular ecology approaches. [less ▲]

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See detailValence des verbes et interdépendances entre lexique et syntaxe en kirundi
Tuyubahe, Pascal ULiege

Doctoral thesis (2017)

Ce travail de recherche a pour objet de décrire des phénomènes lexicaux et syntaxiques du kirundi peu examinés précédemment. Il est subdivisé en deux principales parties : la première partie est consacrée ... [more ▼]

Ce travail de recherche a pour objet de décrire des phénomènes lexicaux et syntaxiques du kirundi peu examinés précédemment. Il est subdivisé en deux principales parties : la première partie est consacrée à l’élaboration d’un lexique valenciel des verbes avec un ou plusieurs compléments, en prenant comme points de départ la liste de lexèmes de de Samie (2008) ainsi que le dictionnaire rundi-français de Rodegem (1970). Il s’avère que beaucoup de verbes peuvent apparaître dans différents schémas de construction, ce qui implique plusieurs unités lexicales pour ces verbes dans le lexique valenciel. La deuxième partie, composée de sept chapitres, consiste en la recherche de généralisations pouvant être faites à partir de ce lexique ; ces généralisations reposant sur une large base empirique, elles sont assez fréquemment accompagnées d’exceptions. Le chapitre 1 concerne le comportement syntaxique des groupes nominaux et groupes prépositionnels dans la valence des verbes et leur caractérisation par des tests; il y est question des objets directs, des objets prépositionnels, des circonstants et des attributs. Un chapitre est consacré au passif impersonnel (chapitre 2) car cette construction fournit une justification purement empirique à la catégorie « objet prépositionnel » en kirundi, permettant de distinguer les objets prépositionnels (locatif ou non) des autres compléments (y compris les circonstants valenciels de lieu). Le chapitre 3 montre l’importance quantitative des objets directs par rapport aux autres objets du lexique valenciel des verbes en kirundi et montre comment cette importance est encore accrue par l’existence en kirundi d’objets directs non valenciels, en particulier les objets introduits par l’intermédiaire d’un suffixe verbal (suffixe applicatif -ir- ou suffixe causatif -i- ou -iish-) et les possesseurs inaliénables introduits sans intermédiaire. Après avoir fait une présentation générale des temps et aspects et de la concordance des temps en kirundi (chapitre 4), les chapitres 5, 6 et 7 montrent comment cela se manifeste dans les propositions subordonnées valencielles et les propositions avec auxiliaire, en distinguant celles qui ont un verbe conjugué et celles qui ont un infinitif. Les conclusions générales sont suivies des propositions prospectives permettant d’effectuer des recherches approfondies en vue de compléter le lexique valenciel, d’une part et, d’autre part, de déterminer les conditions d’emploi des suffixes causatifs -i- et -iish-. [less ▲]

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