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See detailMartian mesospheric cloud observations by IUVSon MAVEN: Thermal tides coupled to the upper atmosphere
Stevens; Siskind; Evans et al

in Geophysical Research Letters (2017), 44

The manuscript describes the observation of Martian mesosphericclouds between 60 and 80 km altitude by the Imaging Ultraviolet Spectrograph (IUVS) on NASA’sMAVEN spacecraft. The cloud observations are ... [more ▼]

The manuscript describes the observation of Martian mesosphericclouds between 60 and 80 km altitude by the Imaging Ultraviolet Spectrograph (IUVS) on NASA’sMAVEN spacecraft. The cloud observations are uniquely obtained at early morning local times, whichcomplement previous observations obtained primarily later in the diurnal cycle. Differences in thegeographic distribution of the clouds from IUVS observations indicate that the local time is crucial for theinterpretation of mesospheric cloud formation. We also report concurrent observations of upperatmospheric scale heights near 170 km altitude, which are diagnostic of temperature. These observationssuggest that the dynamics enabling the formation of mesospheric clouds propagate all the way to theupper atmosphere. [less ▲]

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See detailDeciphering the role of dendritic sodium channels on action potential dynamics in substantia nigra dopaminergic neurons
Moubarak, Estelle; Engel, Dominique ULg; Dufour, Martial et al

Poster (2017, May)

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See detailTransparent Electrodes Based on Silver Nanowire Networks: From Physical Considerations towards Device Integration
Bellet, Daniel; Lagrange, Mélanie; Sannicolo, Thomas et al

in Materials (2017), 10

The past few years have seen a considerable amount of research devoted to nanostructured transparent conducting materials (TCM), which play a pivotal role in many modern devices such as solar cells ... [more ▼]

The past few years have seen a considerable amount of research devoted to nanostructured transparent conducting materials (TCM), which play a pivotal role in many modern devices such as solar cells, flexible light-emitting devices, touch screens, electromagnetic devices, and flexible transparent thin film heaters. Currently, the most commonly used TCM for such applications (ITO: Indium Tin oxide) suffers from two major drawbacks: brittleness and indium scarcity. Among emerging transparent electrodes, silver nanowire (AgNW) networks appear to be a promising substitute to ITO since such electrically percolating networks exhibit excellent properties with sheet resistance lower than 10 Ω/sq and optical transparency of 90%, fulfilling the requirements of most applications. In addition, AgNW networks also exhibit very good mechanical flexibility. The fabrication of these electrodes involves low-temperature processing steps and scalable methods, thus making them appropriate for future use as low-cost transparent electrodes in flexible electronic devices. This contribution aims to briefly present the main properties of AgNW based transparent electrodes as well as some considerations relating to their efficient integration in devices. The influence of network density, nanowire sizes, and post treatments on the properties of AgNW networks will also be evaluated. In addition to a general overview of AgNW networks, we focus on two important aspects: (i) network instabilities as well as an efficient Atomic Layer Deposition (ALD) coating which clearly enhances AgNW network stability and (ii) modelling to better understand the physical properties of these networks. [less ▲]

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See detailPrincipes de contentieux administratif
Pâques, Michel ULg

Book published by Larcier - 1re édition (2017)

L’importance du contentieux administratif n’échappe à personne. La qualité de son traitement donne au droit administratif sa consistance. L’obligation faite à l’administration de s’incliner devant la ... [more ▼]

L’importance du contentieux administratif n’échappe à personne. La qualité de son traitement donne au droit administratif sa consistance. L’obligation faite à l’administration de s’incliner devant la décision du juge permet l’État de droit. Du projet à la pratique, le trajet n’est bien entendu pas toujours rectiligne. La tension entre efficacité, sécurité juridique et légalité est constante, tout comme est ininterrompue la quête d’un meilleur système. En cette matière, les tendances sont longues, mais la réforme est permanente. L’ouvrage a été conçu pour les étudiants de la troisième année du baccalauréat en droit à qui il s’agit de présenter les principes du contentieux, mais aussi les éléments forts du droit positif. Le plan est celui d’un cours, en vue de l’acquisition progressive des connaissances nécessaires à ce qui vient ensuite. La pratique, pour sa part, doit bien entendu tout mettre en œuvre en même temps. L’utilisation de deux types de caractères et de notes permet de viser l’objectif d’une présentation structurante tout en présentant déjà les nuances, les exemples et parfois les conseils immédiatement utiles à la pratique contemporaine. Quelques développements sont consacrés à la manière concrète d’exercer certains recours, comme la formulation d’un moyen dans une requête en annulation. Questions clés : recours administratif, droit au juge et limitations, compétence fédérale, régionale et communautaire en matière de juridiction, droits subjectifs de l’administré, responsabilité de l’administration, faute et illégalité, censure de l’acte illégal, séparation des pouvoirs, pouvoir d’injonction contre l’administration, contentieux objectif et subjectif, Conseil d’État, autorité administrative, recours en annulation, compétence et incompétence du Conseil d’État en raison de l’objet véritable du litige, recevabilité, délais, intérêt au recours et au moyen, référé administratif, annuler ou ne pas annuler l’acte illégal ou limiter les effets de l’annulation, indemnité réparatrice. [less ▲]

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See detailIdentifying novel biomarkers for the rapid diagnosis of invasive aspergillosis using GC×GC-TOFMS
Stefanuto, Pierre-Hugues ULg; Rees, Christiaan A.; Beattie, Sarah R. et al

Conference (2017, May)

Aspergillus fumigatus is a filamentous fungus responsible for the life-threatening infection, invasive aspergillosis (IA), in immunocompromised individuals. Currently, IA diagnostic methods take days to ... [more ▼]

Aspergillus fumigatus is a filamentous fungus responsible for the life-threatening infection, invasive aspergillosis (IA), in immunocompromised individuals. Currently, IA diagnostic methods take days to produce a result and methods to obtain samples are invasive. One approach to improve the time-to-diagnosis is based on utilizing our knowledge of fungal metabolism during pathogenesis. Recent studies have demonstrated the importance of oxygen availability during IA, which presents a potential diagnostic opportunity. In this study, we analyzed the early (1 hr) and late (8 hr) volatile profile of A. fumigatus strain CEA1o under oxygen replete (~20% O2) and deplete (0.2% O2) conditions. In order to comprehensively screen the headspace of these cultures, we applied headspace solid phase microextraction (HS-SPME) associated with comprehensive two-dimensional gas chromatography coupled to time of flight mass spectrometry (GC×GC-TOFMS). Comparing the headspace of the four culture conditions, we were able to identify specific volatile biomarkers of low oxygen fungal growth: ethanol, acetaldehyde, 2-propanol, 2,3-butanedione, and 3-hydroxy-2-butanone. Based on the putative identifications of these compounds, we identified potential metabolic pathways that are responsible for their production. The two first compounds are known to be produced during hypoxia metabolism of glucose, however, the three others suggest that previously uncharacterized metabolic pathways may be utilized by A. fumigatus in low oxygen conditions. Validation of these biomarker identities and translation into patients may generate metabolic insight into these organisms during IA pathogenesis. [less ▲]

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See detailIdentifying infectious agents from lung transplant patients using GC×GC-TOFMS
Stefanuto, Pierre-Hugues ULg; Rees, Christiaan A.; Romamno, Rosalba et al

Poster (2017, May)

During the first year post-surgery, bacterial and fungal infections are the main causes of death in lung transplant recipients. Contributing substantially to the high mortality rate (35%) is the lengthy ... [more ▼]

During the first year post-surgery, bacterial and fungal infections are the main causes of death in lung transplant recipients. Contributing substantially to the high mortality rate (35%) is the lengthy time-to-diagnosis, which includes both pathogen identification and antibiotic resistance profiling. A novel diagnostic strategy based on the detection of volatile compounds in breath seems a promising option to improve these patients’ outcomes. In order to demonstrate the feasibility of this approach, we characterized the volatile fraction of different clinical matrices (i.e., bronchoalveolar lavage (BAL) and blind bronchial aspirate (BBA)). Due to the complexity of the mixture and high dynamic range of the concentrations, we applied headspace solid phase microextraction (HS-SPME) combined with comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry (GC×GC-TOFMS) to obtain a comprehensive overview of the sample headspace composition. Following the analytical step, we applied various univariate and multivariate statistical methods (e.g. random forest, principal component analysis, and hierarchical cluster analysis) in order to extract required information from the data. Based on the composition of the volatile fraction, we were able to differentiate between BAL and BBA with a classification accuracy of 88.4 %. Furthermore, we could correctly distinguish between individuals with active respiratory infections and those without infection with a 72.2 % classification accuracy. In some cases, it was also possible to differentiate between pathogens at the species level. These results are preliminary evidence to suggest the possibility of detecting infections in these patient without the use of time-consuming culture techniques. [less ▲]

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See detailSniffing Out the Hypoxia Volatile Metabolic Signature of Aspergillus fumigatus
Stefanuto, Pierre-Hugues ULg; Rees, Christiaan A.; Beattie, Sarah R. et al

Poster (2017, May)

Background: Aspergillus fumigatus is responsible for the life-threatening infection invasive aspergillosis (IA) that is associated with high mortality in immunocompromised individuals. Current diagnostics ... [more ▼]

Background: Aspergillus fumigatus is responsible for the life-threatening infection invasive aspergillosis (IA) that is associated with high mortality in immunocompromised individuals. Current diagnostics for IA are either highly invasive or suffer from inadequate sensitivity and/or specificity. Improvement of IA diagnostic assays requires a better understanding of fungal metabolism inside the host. The infection microenvironment becomes progressively depleted of oxygen during IA. Thus, metabolites associated with hypoxia metabolism may yield to novel diagnostic biomarkers. Here, we report on the volatile metabolites emitted from A. fumigatus batch cultures under normoxia and hypoxia conditions. Methods: A. fumigatus strain CEA10 was incubated in glucose minimal medium at 37 °C under either normoxia (21% O2, 0.04% CO2) or hypoxia (0.2% O2, 5% CO2) growth conditions. Volatile molecules from the supernatants of early (1 h) and late (8 h) cultures were collected using headspace solid-phase microextraction (HS-SPME) and analyzed using two-dimensional gas chromatography-time-of-flight mass spectrometry (GCxGC-TOFMS). Results: We identified 10 volatile compounds that are significantly more abundant in late hypoxia cultures compared with all other experimental conditions. Among these compounds, we identified ethanol and acetaldehyde, which are known to result from hypoxia metabolism of glucose by A. fumigatus. In addition, we detected 2-propanol, which can be generated by the reduction of acetone via alcohol dehydrogenase, as well as 2,3-butanedione (diacetyl) and 3-hydroxy-2-butanone (acetoin), which results from the catabolism of acetolactate. Transcriptomic data collected from normoxia and hypoxia cultures validates our volatilomic findings through the identification of putative catabolic pathways that are upregulated during hypoxia growth. Diacetyl is known to be produced by bacteria as a pH-neutral fermentative byproduct to prevent lethal acidification, and we speculate that A. fumigatus may use the same pathway. Conclusion: The composition of A. fumigatus’ volatile metabolome changes according to the oxygen tension in the environment. We measured hypoxia-associated compounds, ethanol and acetaldehyde which have been previously identified as byproducts of hypoxia metabolism in this organism. 2-propanol, 2, 3-butanedione and 3-hydroxy-2-butanone represent novel metabolites that suggests the existence of other catabolic pathways activated in A. fumigatus under hypoxia growth conditions. [less ▲]

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See detailTargeting of C-type lectin-like receptor 2 or P2Y12 for the prevention of platelet activation by immunotherapeutic CpG oligodeoxynucleotides
Delierneux, Céline ULg; Donis, Nathalie ULg; servais, laurence et al

in Journal of Thrombosis and Haemostasis (2017), 15(5), 983-997

Background: Synthetic phosphorothioate-modified CpG oligodeoxynucleotides (CpG ODNs) display potent immunostimulatory properties that are widely exploited in clinical trials of anticancer treatment ... [more ▼]

Background: Synthetic phosphorothioate-modified CpG oligodeoxynucleotides (CpG ODNs) display potent immunostimulatory properties that are widely exploited in clinical trials of anticancer treatment. Unexpectedly, a recent study indicates that CpG ODNs activate human platelets via the immunoreceptor tyrosine-based activation motif (ITAM)-coupled receptor glycoprotein VI. Objective: To further analyze the mechanisms of CpG ODN-induced platelet activation and identify potential inhibitory strategies. Methods: In vitro analyses were performed on human and mouse platelets, and on cell lines expressing platelet ITAM receptors. CpG ODN platelet activating effects were evaluated in a mouse model of thrombosis. Results: We demonstrated platelet uptake of CpG ODNs, resulting in platelet activation and aggregation. The C-type lectin-like receptor 2 (CLEC-2) expressed in DT40 cells bound CpG ODNs. CpG ODN uptake did not occur in CLEC-2-deficient mouse platelets. Inhibition of human CLEC-2 with a blocking antibody inhibited CpG ODN-induced platelet aggregation. CpG ODNs caused CLEC-2 dimerization, and provoked its internalization. They induced dense granule release before the onset of aggregation. Accordingly, pretreating platelets with apyrase, or inhibiting P2Y12 with cangrelor or clopidogrel prevented CpG ODN platelet activating effect. In vivo, intravenously injected CpG ODN interacted with platelets adhered to mouse injured endothelium, and promoted thrombus growth, which was inhibited by CLEC-2 deficiency or by clopidogrel. Conclusions: CLEC-2 and P2Y12 are required for CpG ODN-induced platelet activation and thrombosis and might be targeted to prevent adverse events in patients at risk. [less ▲]

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See detailA Highly Durable RNAi Therapeutic Inhibitor of PCSK9
LANCELLOTTI, Patrizio ULg; Oury, Cécile ULg

in New England Journal of Medicine (2017), 376

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See detailContinental Climate Gradients in North America and Western Eurasia before and after the Closure of the Central American Seaway
Utescher, Torsten; Dreist, Andreas; Henrot, Alexandra-Jane ULg et al

in Earth and Planetary Science Letters (2017), 472

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See detailLe boom des maladies auto-immunes.
Luong, Julie; GEENEN, Vincent ULg

Article for general public (2017)

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See detailCo-creating service recovery after service failure: The role of brand equity
Hazee, Simon ULg; Van Vaerenbergh, Yves; Armirotto, Vincent

in Journal of Business Research (2017), 74

Co-creating service recovery with customers has recently appeared as a new perspective in service research. Prior research demonstrates the effectiveness of co-created recovery strategies in driving ... [more ▼]

Co-creating service recovery with customers has recently appeared as a new perspective in service research. Prior research demonstrates the effectiveness of co-created recovery strategies in driving customer outcomes, and out- lines when co-creating a service recovery is recommended. This paper complements prior research not only by demonstrating the mediating role of outcome favorability in the relationship between co-created service recov- ery and customer outcomes, but also by showing whether organizations with different levels of brand equity benefit equally from co-creating service recovery with their customers. The results of two experiments show that co-creating a service recovery makes customers believe they received the most favorable solution for the ser- vice failure, which in turn influences satisfaction with service recovery and repurchase intentions. In addition, co- creating a service recovery is recommended for organizations with low levels of brand equity, but not for orga- nizations with high levels of brand equity. [less ▲]

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See detailDiscovery and characterization of EIIB, a new α-conotoxin from Conus ermineus venom by nAChRs affinity capture monitored by MALDI-TOF/TOF mass spectrometry
Echterbille, Julien; Gilles, Nicolas; Araoz, Romulo et al

in Toxicon (2017), 130

Animal toxins are peptides that often bind with remarkable affinity and selectivity to membrane receptors such as nicotinic acetylcholine receptors (nAChRs). The latter are, for example, targeted by α ... [more ▼]

Animal toxins are peptides that often bind with remarkable affinity and selectivity to membrane receptors such as nicotinic acetylcholine receptors (nAChRs). The latter are, for example, targeted by α-conotoxins, a family of peptide toxins produced by venomous cone snails. nAChRs are implicated in numerous physiological processes explaining why the design of new pharmacological tools and the discovery of potential innovative drugs targeting these receptor channels appear so important. This work describes a methodology developed to discover new ligands of nAChRs from complex mixtures of peptides. The methodology was set up by the incubation of Torpedo marmorata electrocyte membranes rich in nAChRs with BSA tryptic digests (>100 peptides) doped by small amounts of known nAChRs ligands (α-conotoxins). Peptides that bind to the receptors were purified and analyzed by MALDI-TOF/TOF mass spectrometry which revealed an enrichment of α-conotoxins in membrane-containing fractions. This result exhibits the binding of α-conotoxins to nAChRs. Negative controls were performed to demonstrate the specificity of the binding. The usefulness and the power of the methodology were also investigated for a discovery issue. The workflow was then applied to the screening of Conus ermineus crude venom, aiming at characterizing new nAChRs ligands from this venom, which has not been extensively investigated to date. The methodology validated our experiments by allowing us to bind two α-conotoxins (α-EI and α-EIIA) which have already been described as nAChRs ligands. Moreover, a new conotoxin, never described to date, was also captured, identified and sequenced from this venom. Classical pharmacology tests by radioligand binding using a synthetic homologue of the toxin confirm the activity of the new peptide, called α-EIIB. The Ki value of this peptide for Torpedo nicotinic receptors was measured at 2.2 ± 0.7 nM. [less ▲]

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See detailBuilding an Effective Strategy To Supply High Frequency Stores in Emerging Cities
Sabiri, Asmaa; Riane, Fouad; Limbourg, Sabine ULg

in Cools, Mario; Limbourg, Sabine (Eds.) Proceedings of the BIVEC-GIBET Transport Research Days 2017: Towards an Autonomous and Interconnected Transport Future (2017)

Traditional distribution channels that cover nanostores still abound in merging megacities. These traditional retail formats need to be visited and supplied very frequently (High Frequency Stores) which ... [more ▼]

Traditional distribution channels that cover nanostores still abound in merging megacities. These traditional retail formats need to be visited and supplied very frequently (High Frequency Stores) which turns to be costly and time consuming if not well optimized. Supplying High Frequency Stores in emerging countries involves different constraints that make the associated optimization problems complex. In this paper, we deal with a real-life application problem where a company try to build an effective strategy to cover and to supply nanostores. We present an altered approach to a multi-depot multiple traveling salesman problem. The problem of concern is modeled as an assignment problem, combined with side constraints regarding disruption of pre-assignments and profit potential balance. The results show that the multi-objective function produces better profit and workload balance than setting a simple objective function and a constraint, one for the profit balance and the other for workload balance and vice-versa. [less ▲]

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See detailThe Fleet Size and Mix Vehicle Routing Problem with sequence-based pallet loading and axle weight constraints
Pollaris, Hanne; Braekers, Kris; caris, An et al

in Cools, Mario; Limbourg, Sabine (Eds.) Proceedings of the BIVEC-GIBET Transport Research Days 2017: Towards an Autonomous and Interconnected Transport Future (2017)

In this paper, the Fleet Size and Mix Vehicle Routing Problem (FSM) with sequence-based pallet loading and axle weight constraints is introduced. The effect of the integration of axle weight constraints ... [more ▼]

In this paper, the Fleet Size and Mix Vehicle Routing Problem (FSM) with sequence-based pallet loading and axle weight constraints is introduced. The effect of the integration of axle weight constraints in a FSM is analyzed by comparing the FSM with and without axle weight constraints. A vehicle fleet of 30-foot and 45-foot trucks, consisting of a tractor and a semi-trailer, is considered. Furthermore, two scenarios are analyzed for which the objective function differs: in the first scenario the objective is to minimize total distance while in the second scenario the objective is the minimization of total transport costs. An Iterated Local Search metaheuristic algorithm is used to tackle the problem. The results indicate that the vehicle fleet strongly influences the impact of the integration of axle weight constraints on solution cost. Furthermore, the analysis points out that fleet size and mix decisions may be influenced by the integration of axle weight constraints. [less ▲]

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See detailEvaluation of the implementation of integrated care for people with chronic conditions
Van Durme, Thérèse; Anthierens, Sibyl; Van Hecke, Ann et al

Conference (2017, May)

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See detailDiversity in sequences, post-translational modifications and expected pharmacological activities of toxins from four Conus species revealed by the combination of cutting-edge proteomics, transcriptomics and bioinformatics
Degueldre, Michel; Verdenaud, Marion; Garikoitz, Legarda et al

in Toxicon (2017), 130

Venomous animals have developed a huge arsenal of reticulated peptides for defense and predation. Based on various scaffolds, they represent a colossal pharmacological diversity, making them top ... [more ▼]

Venomous animals have developed a huge arsenal of reticulated peptides for defense and predation. Based on various scaffolds, they represent a colossal pharmacological diversity, making them top candidates for the development of innovative drugs. Instead of relying on the classical, low-throughput bioassay-guided approach to identify innovative bioactive peptides, this work exploits a recent paradigm to access to venom diversity. This strategy bypasses the classical approach by combining high-throughput transcriptomics, proteomics and bioinformatics cutting-edge technologies to generate reliable peptide sequences. The strategy employed to generate hundreds of reliable sequences from Conus venoms is deeply described. The study led to the discovery of (i) conotoxins that belong to known pharmacological families targeting various GPCRs or ion-gated channels, and (ii) new families of conotoxins, never described to date. It also focusses on the diversity of genes, sequences, folds, and PTM's provided by such species. [less ▲]

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