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See detailChitin-glucan complex production by Komagataella pastoris: downstream optimization and product characterization
Farinha, Inês; Duarte, Paulo; Pimentel, Ana et al

in Carbohydrate Polymers (in press)

Purified chitin-glucan complex (CGCpure) was extracted from Komagataella pastoris biomass using a hot alkaline treatment, followed by neutralization and repeated washing with deionised water. The co ... [more ▼]

Purified chitin-glucan complex (CGCpure) was extracted from Komagataella pastoris biomass using a hot alkaline treatment, followed by neutralization and repeated washing with deionised water. The co-polymer thus obtained had a glucan:chitin molar ratio of 75:25 and low protein and inorganic salts contents (3.0 and 0.9 wt%, respectively). CGCpure had an average molecular weight of 4.9 × 105 Da with a polydispersity index of 1.7, and a crystallinity index of 50%. Solid-state NMR provided structural insight at the co-polymer. X-ray diffraction and FTIR analysis suggest that CGCpure has b-chitin in its structure. CGCpure presented an endothermic decomposition peak at 315 oC, assigned to the degradation of the saccharide structures. This study revealed that K. pastoris CGC has properties similar to other chitinous biopolymers and may represent an attractive alternative to crustacean chitin derived-products, being a reliable raw material for the development of new/improved pharmaceutical, cosmetic or food products. [less ▲]

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See detailA radio-map of the colliding winds in the very massive binary system HD93129A
Benaglia, Paula; Marcote, Benito; Moldon, Javier et al

in Astronomy and Astrophysics (in press)

Radio observations are an effective tool to discover particle acceleration regions in colliding-wind binaries, through detection of synchrotron radiation; these regions are natural laboratories for the ... [more ▼]

Radio observations are an effective tool to discover particle acceleration regions in colliding-wind binaries, through detection of synchrotron radiation; these regions are natural laboratories for the study of relativistic particles.Wind-collision region (WCR) models can reproduce the radio continuum spectra of massive binaries that contain both thermal and non-thermal radio emission; however, key constraints for models come from high-resolution imaging. Only five WCRs have been resolved to date at radio frequencies at milliarcsec (mas) angular scales. The source HD93129A, prototype of the very few known O2 I stars, is a romising target for study: recently, a second massive, early-type star about 50 mas away was discovered, and a non-thermal radio source detected in the region. Preliminary long-baseline array data suggest that a significant fraction of the radio emission from the system comes from a putative WCR. We sought evidence that HD93129A is a massive binary system with colliding stellar winds that produce non-thermal radiation, through spatially resolved images of the radio emitting regions. Methods.We completed observations with the Australian Long Baseline Array (LBA) to resolve the system at mas angular resolutions and reduced archival Australia Telescope Compact Array (ATCA) data to derive the total radio emission. We also compiled optical astrometric data of the system in a homogeneous way. We reduced historical Hubble Space Telescope data and obtained absolute and relative astrometry with milliarcsec accuracy. The astrometric analysis leads us to conclude that the two stars in HD93129A form a gravitationally bound system. The LBA data reveal an extended arc-shaped non-thermal source between the two stars, indicative of a WCR. The wind momentum-rate ratio of the two stellar winds is estimated. The ATCA data show a point source with a change in flux level between 2003-4 and 2008-9, that is modeled with a non-thermal power-law spectrum with spectral indices of - 1:03 +/- 0:09 and -1:21 +/- 0:03 respectively. The mass-loss rates derived from the deduced thermal radio emission and from the characteristics of the WCR are consistent with estimates derived by other authors. [less ▲]

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See detailArticle "Hippocrate"
Ricciardetto, Antonio ULg

in Zawieja, Philippe (Ed.) Dictionnaire de la fatigue (in press)

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See detailArticle "Galien"
Ricciardetto, Antonio ULg

in Zawieja, Philippe (Ed.) Dictionnaire de la fatigue (in press)

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See detailSe faire « manger par l’autre ». Stratégie féminines d’affirmation de soi en contexte public.
Mescoli, Elsa ULg

in Etrangers au Maghreb. Maghrébins à l’étranger : politiques publiques à l’épreuve de la société civile : XIXe-XXIe siècles. Actes du colloque. (in press)

Cette communication vise à étudier la participation des femmes migrantes marocaines à la vie sociale du « pays d’accueil » ; participation qui se réalise grâce aux activités de la vie quotidienne. En ... [more ▼]

Cette communication vise à étudier la participation des femmes migrantes marocaines à la vie sociale du « pays d’accueil » ; participation qui se réalise grâce aux activités de la vie quotidienne. En particulier, la performance publique des pratiques culinaires, observée dans une ville de la province de Milan, permet aux femmes considérées d’exercer une agency à l’intérieur des espaces institutionnellement concédés à l’expression des migrants. D’un côté, la consommation des « recettes de l’Autre » de la part de la population majoritaire détermine une incorporation de la diversité afin de la contrôler. De l’autre côté, la préparation de ces recettes consent aux migrantes d’exprimer leur subjectivité (individuelle et collective) de façon active et en présentant des aspects qui vont au-delà de la culture alimentaire. Une participation sociale fondée sur le partage de nourriture donne la possibilité aux femmes d’assumer un rôle actif dans la communauté locale, tout en affirmant ses propres spécificités. Des sujets tels que la liberté religieuse sont abordés, et certains droits peuvent être réclamés par des individus qui ont ainsi acquis une position politiquement importante au sein de la société locale. [less ▲]

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See detailCountries of origin as organisers of emigration: Moroccans and Turks in Belgium.
Gsir, Sonia ULg; Mandin, Jérémy ULg; Mescoli, Elsa ULg

in Fargues, P.; Weinar, A.; Di Bartolomeo, A. (Eds.) et al From Home to Home? How countries of origin impact immigrant integration outcomes, volume 2 (empirical findings), (in press)

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See detailDes “salauds”. Ethos et interdiscours dans Causeur
Provenzano, François ULg

in Durand, Pascal; Sindaco, Sarah (Eds.) Les « Nouveaux Réactionnaires ». Genèses, configurations, discours (in press)

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See detailStéphane Mallarmé (1842-1898)
Durand, Pascal ULg

in Sapiro, Gisèle (Ed.) Dictionnaire international Bourdieu (in press)

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See detailAbelian bordered factors and periodicity
Charlier, Emilie ULg; Harju, Tero; Puzynina, Svetlana et al

in European Journal of Combinatorics (in press)

A finite word u is said to be bordered if u has a proper prefix which is also a suffix of u, and unbordered otherwise. Ehrenfeucht and Silberger proved that an infinite word is purely periodic if and only ... [more ▼]

A finite word u is said to be bordered if u has a proper prefix which is also a suffix of u, and unbordered otherwise. Ehrenfeucht and Silberger proved that an infinite word is purely periodic if and only if it contains only finitely many unbordered factors. We are interested in abelian and weak abelian analogues of this result; namely, we investigate the following question(s): Let w be an infinite word such that all sufficiently long factors are (weakly) abelian bordered; is w (weakly) abelian periodic? In the process we answer a question of Avgustinovich et al. concerning the abelian critical factorization theorem. [less ▲]

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See detailUPLC®-MS/MS, an analytical tool for the accurate and rapid quantification of phytoestrogen metabolites in milk
Daems, Frédéric ULg; Jasselette, Christophe; Romnee, Jean-Michel et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (in press)

The term ‘phytoestrogen’ is used to define a wide variety of nonsteroidal compounds that occur naturally in many plants. When they are absorbed by cows, these polyphenolic compounds undergo ... [more ▼]

The term ‘phytoestrogen’ is used to define a wide variety of nonsteroidal compounds that occur naturally in many plants. When they are absorbed by cows, these polyphenolic compounds undergo biotransformation by specific bacteria present in the gastrointestinal tract. The resulting metabolites are absorbed in the organism and some are excreted in milk. The impact of these compounds on human health divides opinion. Some scientists believe that they might have adverse health effects. Others believe the opposite, especially with regard to their microbial metabolites, such as equol. Whatever one's view on the subject, scientists need accurate, sensitive and rapid analytical methods in order to continue the research on clarifying the phytoestrogen issue. Cow's milk is an animal product that is common in the human diet, and it is therefore important to evaluate its content of phytoestrogen metabolites. In order to study the human intake of equol via commercial milk, an analytical method was developed and validated following EMA/CVMP/VICH/463202/2009 guidelines. Enzymatic hydrolysis was used to release the equol. It was then extracted using double liquid/liquid extraction and analyzed using UPLC®-MS/MS, with an analysis runtime of only 5 min. This analytical method produced a linear calibration curve with a high correlation coefficient (R2≥0.996) between 5 and 1,000 ng mL−1. Good intra- and inter-day precision (RSDs≤5.3% and ≤5.2%, respectively) and accuracy (bias≤8.6%) were achieved. The recovery rate differed slightly among the different types of milk, ranging between 60.6±1.09% and 82.3±5.21%. Good method repeatability was observed (RSDs<15%). There was neither a matrix effect nor a carry-over effect, and the sample extracts were stable during storage for at least 7 days at −21°C and 5°C. In order to apply the proposed method and obtain an initial estimate of equol concentration in cow’s milk in Belgium, 44 samples of various brands found in several supermarkets and 5 raw milk samples from Walloon farms were analyzed. Equol was found in all the samples analyzed, with a concentration ranging from 10 to 50 ng mL−1 for conventional milk and from 70 to 130 ng mL−1 for organic milk. These results were compared with other European studies and the same trend was observed. The results obtained show the specificity, sensitivity and precision of this method for analyzing oestrogenic metabolite-equol in cow’s milk. This study should be extended to include a greater number of samples and be implemented over a longer period to see if there is a correlation between livestock practice and/or sample origins with the equol content in milk. The addition of other phytoestrogen compounds with this method, using UPLC®-MS/MS technology, could also lead to more reliable monitoring of these compounds in dairy production. [less ▲]

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See detailQue peut la rhétorique pour les études francophones ?, ou comment étudier les enjeux de catégorisation et d’institutionnalisation
Provenzano, François ULg

in Anders Baggesgaard, Mads; Verstraete-Hansen, Lisbeth (Eds.) Le monde en français – les littératures francophones dans un espace mondialisé (in press)

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See detailProjections photographiques et cinéma des premiers temps : La houillère de Gustave Marissiaux et les origines du cinéma minier
Mélon, Marc-Emmanuel ULg

in Intermédialités : Histoire et Théorie des Arts, des Lettres et des Techniques = Intermediality: History and Theory of the Arts, Literature and Technologies (in press)

Les spectacles de projection conçus par Gustave Marissiaux entre 1903 et 1926, en particulier le vaste documentaire intitulé "La houillère" (1905) est ici inscrit dans une histoire sociale des images ... [more ▼]

Les spectacles de projection conçus par Gustave Marissiaux entre 1903 et 1926, en particulier le vaste documentaire intitulé "La houillère" (1905) est ici inscrit dans une histoire sociale des images projetées et mis en rapport avec le cinéma minier des premiers temps [less ▲]

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See detailSomatotrope GHRH/GH/IGF-1 axis at the crossroad between immunosenescence and elder frailty
Bodart, Gwennaëlle ULg; Goffinet, Lindsay; Morrhaye, Gabriel et al

in Annals of the New York Academy of Sciences (in press)

Immunosenescence as complex modifications of immunity with age could be related to the so-called frailty syndrome of elderly leading to an inadequate response to minimal aggression. Functional decline ... [more ▼]

Immunosenescence as complex modifications of immunity with age could be related to the so-called frailty syndrome of elderly leading to an inadequate response to minimal aggression. Functional decline, the loss of ability to perform activities of daily living, is related to the decrease in physiological reserves and frailty and is a frequent outcome of hospitalization in older patients. Links between immunosenescence and frailty were explored and 20 immunological parameters were affected in seniors with functional decline. IGF-1, thymopoeisis and telomere length were part of these markers. A strong relationship between insulin-like growth factor-1 (IGF-1) and thymic ouput was evidenced. IGF-1, mediator of GH, was subsequently shown to induce IL-7 secretion in cultured primary human thymic epithelial cells (TECs). We are also exploring the ‘stress hypothesis’ according which an acute stress is the discriminator revealing a frailty susceptility. GH can counteract the deleterious immunosuppressive effect of stress-induced steroids. Under non-stressing conditions, the immunosenescent system preserves physiological responses, while in stressing conditions, the combination of immunosenescence and a defect in somatotrope axis might lead to functional decline. [less ▲]

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See detailIl Bibbiena ‘moccicone’: l’autoironia e il classicismo come ingredienti del perfetto segretario e cortigiano
Marconato, Claudia ULg

in Être homme de « lettres » : secrétaires et politique culturelle au seizième siècle (in press)

Bernardo Dovizi, one of the most famous secretaries of the early Italian Reinassance, is best known for his literary works and for being the patron of Raffaello rather than for his endeavors in diplomacy ... [more ▼]

Bernardo Dovizi, one of the most famous secretaries of the early Italian Reinassance, is best known for his literary works and for being the patron of Raffaello rather than for his endeavors in diplomacy. By deciding to follow the interests of Giovanni de’ Medici, Dovizi made some poor political decisions in his career but, in spite of this, he cunningly managed to get his patron become the Pope. The "Calandra" is a work that depicts well the author’s ideas, in between parody and classicism. From his works, Epistolario and Ricordi, it appears that the main strength of Dovizi is his irony, through which he manages to become almost an accomplice with his patron. Alongside with the iconological choice of the frescoes of his Stufetta and Loggia in the Vatican, he assumes the posture of the secretary-jester, as it was portrayed by Baldassarre Castiglione in "The Book of the Courtier": the satyr that washes into the room, used as bathroom, corresponds to the most famous pose of the patron who insists on these burlesque and mocking characteristics, although relating to Ovid and Plato. Bibbiena seems to be aware of how much the man that exceeds in praise himself appears unbearable, therefore, in his relations with members of the ruling class, he often assumes a playful attitude. In the Rome of Leo X, because of the Socratic 'serio ludere', such attitude was accepted and admired. The best instruments of Bibbiena are the pleasantness and a careful selection of humanistic ideals. Such ideals were those that suited better the environment in which he, the courtier, had to operate, and were used in his works. [less ▲]

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See detailProblemi filologici per l'edizione di un epistolario cinquecentesco: il codice G 387 (277) della Biblioteca Bertoliana di Vicenza
Marconato, Claudia ULg

in CONVEGNO DELLA SFLI – SOCIETÀ DEI FILOLOGI DELLA LETTERATURA ITALIANA (in press)

Il manoscritto G 387 (277) della Biblioteca Bertoliana di Vicenza solleva alcune questioni relative alla filologia dei carteggi e alla cosiddetta 'filologia materiale: il codice contiene infatti un ... [more ▼]

Il manoscritto G 387 (277) della Biblioteca Bertoliana di Vicenza solleva alcune questioni relative alla filologia dei carteggi e alla cosiddetta 'filologia materiale: il codice contiene infatti un epistolario, intitolato «Libri tre delle lettere di M. Sebastiano Erizzo», oltre ad alcuni discorsi dello stesso autore, un erudito antiquario veneziano che negli ultimi anni ha riscosso un significativo interesse da parte di studiosi di diversa provenienza disciplinare. Sebastiano Erizzo è infatti l’artefice di una svariata serie di volumi a stampa: noto soprattutto per il suo trattato sulle medaglie degli antichi, fu un raffinato traduttore dal greco e un appassionato filosofo e si cimentò anche nella novellistica (»Le sei giornate»). Il codice nel quale sono conservate le lettere erizziane induce in particolare a interrogarsi sulla sua genesi: l’architettura sapiente con la quale sono state divise e disposte le missive, le nove mani che si alternano e la fascicolazione disordinata del manoscritto, unite alla volontà di stampa espressa in due lettere permettono di ipotizzare che esso fosse un libro pronto per la pubblicazione. La valutazione di una molteplicità di fattori contestuali dati dal testimone unico, specie in riferimento alla produzione, alla copia ed eventualmente alla fruizione del testo d’autore, ci permette di riproporre la costellazione di snodi critici che s’intrecciano e che ci spingono a discutere nuovamente i parametri dell’edizione critica del testo, strettamente connessi con il genere e lo stile del documento epistolare, ma anche pertinenti ad un uso tutto erizziano di fenomeni quali la paragrafatura, la punteggiatura, la segmentazione 'tipografica' del testo, che sollevano problemi di ordine pratico e teorico di notevole portata. [less ▲]

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