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See detailTime, preference and intensity: A contrastive study of rather (than) and eerder (dan)
Ghesquière, Lobke; Brems, Lieselotte ULiege

Conference (2017, May 24)

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See detailStreptomyces from Moonmilk, an Untapped Source of New Bioactive Compounds
Martinet, Loïc ULiege; Maciejewska, Marta; Adam, Delphine ULiege et al

Poster (2017, May 24)

Moonmilk speleothems of limestone caves host a rich microbiome, among which Actinobacteria represent one of the most abundant phyla. Ancient medical texts reported that moonmilk had therapeutical ... [more ▼]

Moonmilk speleothems of limestone caves host a rich microbiome, among which Actinobacteria represent one of the most abundant phyla. Ancient medical texts reported that moonmilk had therapeutical properties, thereby suggesting that its filamentous endemic actinobacterial population might be a source of natural products useful in human treatment. In this work, a screening approach was undertaken in order to isolate cultivable Actinobacteria from moonmilk of the Grotte des Collemboles in Belgium, to evaluate their taxonomic profile, and to assess their potential in biosynthesis of antimicrobials. Phylogenetic analysis revealed that all 78 isolates were exclusively affiliated to the genus Streptomyces and clustered into 31 distinct phylotypes displaying various pigmentation patterns and morphological features. Phylotype representatives were tested for antibacterial and antifungal activities and their genomes were mined for secondary metabolite biosynthetic genes coding for non-ribosomal peptide synthetases (NRPSs), and polyketide synthases (PKS). The moonmilk Streptomyces collection was found to display strong inhibitory activities against a wide range of reference organisms, as 94, 71, and 94% of the isolates inhibited or impaired the growth of Gram-positive, Gram-negative bacteria, and fungi, respectively. Interestingly, 90% of the cave strains induced strong growth suppression against the multi-drug resistant Rasamsonia argillacea, a causative agent of invasive mycosis in cystic fibrosis and chronic granulomatous diseases. We are currently identifying molecules responsible for the observed antimicrobial activities which, next to few known bioactive compounds, also reveals many active compounds with molecular masses that do not correspond to known antibiotic deposited in web biomolecules databases such as KNapSAcK, Pubchem, Chemspider. The challenge is to increase the production yields of these unknown compounds, and purify them to further characterize their structure by NMR. Overall, our work supports the common belief that moonmilk might effectively treat various infectious diseases thanks to the presence of a highly diverse population of prolific antimicrobial producing Streptomyces, and thus may indeed constitute a promising reservoir of potentially novel active natural compounds. [less ▲]

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See detailMultifunctional poly(ionic liquid)s: from synthesis to applications in energy and environment
Detrembleur, Christophe ULiege; Patil, Nagaraj ULiege; Debuigne, Antoine ULiege et al

Conference (2017, May 24)

Poly(ionic liquid)s (PILs) are a subclass of polyelectrolytes that gained an enabling role in many fields of polymer chemistry and material science. PILs combine the unique properties of ionic liquids ... [more ▼]

Poly(ionic liquid)s (PILs) are a subclass of polyelectrolytes that gained an enabling role in many fields of polymer chemistry and material science. PILs combine the unique properties of ionic liquids with the flexibility and properties of macromolecules, and provide novel attractive functions. Recently, the precision design of novel PILs by controlled/living polymerization (CLP) techniques was intensively searched for developing emerging applications. This talk will first discuss recent routes for the precision synthesis of all vinyl-imidazolium based (co)polymers in water or in organic media under non-demanding experimental conditions. We will then describe the preparation of innovative redox and surface active PILs, and show the potential of these PILs in battery applications and for multifunctional coatings. More specifically, we will show how macromolecular engineering can be exploited for designing innovative polymer cathodes for ultra-high performance Li storage with unprecedented performances (high capacities and ultra-long life-span over more than 3000 cycles at an extreme current-rate). This innovative and effective molecular design for polymer cathodes opens up new horizons in developing an economical and environmentally benign platform for large-scalable fabrication of high performance batteries. [less ▲]

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See detailAn anticancer molecule stemming from a plant root produced in a bioreactor
Vassaux, Antoine; Tarayre, Cédric ULiege; Delvigne, Frank ULiege et al

Poster (2017, May 24)

Secondary metabolites produced by plants or their symbionts have already shown specific properties: anticancer, anti-inflammatory, antibacterial, antifungal effects, etc. Astin C is a non-ribosomal ... [more ▼]

Secondary metabolites produced by plants or their symbionts have already shown specific properties: anticancer, anti-inflammatory, antibacterial, antifungal effects, etc. Astin C is a non-ribosomal peptide (secondary metabolite) produced by the fungus Villosirosea asteris, endosymbiont of the medicinal plant Aster tataricus, which has shown an interesting anticancer activity. The current challenge is the production of the molecule on a large scale and in higher quantities, either from the original fungus through fermentation technologies, or by a heterologous yeast strain having integrated the genes involved in the astin C biosynthesis pathway. The purpose of this workshop is to highlight the implementation possibilities of a strain producing a metabolite of interest. [less ▲]

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See detailPhenotyping of Brassica napus L. plantlets affected during in vitro growth by the presence of epoxiconazole.
Durenne, Bastien ULiege; Blondel, Alodie; Ducat, Nathalie et al

in 7th International Symposium on Brassicas Abstract Book (2017, May 24)

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See detailCe que devait encore révéler le site de la Schola del Traiano à Ostia Antica
Morard, Thomas ULiege

Conference (2017, May 24)

Detailed reference viewed: 19 (1 ULiège)
See detailThe Matrix of Academic Organizing
Dubois-Shaik, Farah; Dubois, Christophe ULiege

Scientific conference (2017, May 24)

The aim of this paper is twofold. It firstly aims to account for the Matrix of Excellence, Funding and Omnipresence characterizing academic organizations, and more precisely their constitutive three ... [more ▼]

The aim of this paper is twofold. It firstly aims to account for the Matrix of Excellence, Funding and Omnipresence characterizing academic organizations, and more precisely their constitutive three dimensions: managerial discourse, formal and parallel structures (Friedberg, 2012). Secondly, drawing on the metaphor of shadow organizing (Gherardi et al., 2017), we will illustrate some informal coping mechanisms occurring in the parallel structure of academic organizations. We will then discuss how these coping mechanisms are or are not being translated in managerial discourse and inscribed in formal structures. [less ▲]

Detailed reference viewed: 47 (2 ULiège)
See detailPolyphosphoesters: new trends in synthesis and drug delivery applications
Jérôme, Christine ULiege

Conference (2017, May 24)

Detailed reference viewed: 18 (2 ULiège)
See detailTh17 cells impact on xenogeneic graft-versus-host disease
Delens, Loïc ULiege; SERVAIS, Sophie ULiege; Ehx, Grégory ULiege et al

Poster (2017, May 24)

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See detailStrengthening capacities to enhance food safety in low and middle income countries (LMIC)
Schiffers, Bruno ULiege

Conference given outside the academic context (2017)

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See detailDevelopment of non-linear Electro-Thermo-Mechanical Discontinuous Galerkin formulations
Homsi, Lina ULiege

Doctoral thesis (2017)

A coupled Electro-Thermo-Mechanical Discontinuous Galerkin (DG) method is developed considering the non-linear interactions of electrical, thermal, and mechanical fields. The numerical properties of the ... [more ▼]

A coupled Electro-Thermo-Mechanical Discontinuous Galerkin (DG) method is developed considering the non-linear interactions of electrical, thermal, and mechanical fields. The numerical properties of the DG are demonstrated, such as uniqueness, consistency, stability and the optimal convergence rate. The framework is applied to simulate the response of smart composite materials, where the shape memory effect is triggered by the Joule effect. [less ▲]

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See detailUsing crop modelling to determine the meteorological conditions to be implemented in an Ecotron facility - Prerequisites to improve the experimental design?
Dumont, Benjamin ULiege; Leemans, Vincent ULiege; Garré, Sarah ULiege et al

Poster (2017, May 23)

An Ecotron is a facility where ecosystems are confined in experimental chambers, allowing the simultaneous control of environmental conditions and the on-line monitoring of processes. Under the threats of ... [more ▼]

An Ecotron is a facility where ecosystems are confined in experimental chambers, allowing the simultaneous control of environmental conditions and the on-line monitoring of processes. Under the threats of climate change and the pressure of a world growing population, such facilities will be of major importance to study the relations between climate change and agro-ecosystems.As it can quickly become time- and money-consuming, conducting experiments in an Ecotron will force researchers to cautiously select the climate of interest to be generated. They will thus need reliable tools to help them support the decision making process.Here, we present an innovative methodology, supported by the use of crop model, to assist researchers in finding the climatic conditions under which crop services will be impacted.The meteorological datasets among which the choice can be done were generated by the ALARO-0 model (RMI, Belgium) for current and future climatic conditions. Runs were conducted for the historical period 1981-2010, and for two time frames - 2041-70 and 2071-2100 - under two emissions scenarios - RCP 4.5 and 8.5.A crop model (STICS, INRA, FR) was run over the entire database. Crop model outputs were synthesized for the main crop phenological phases, i.e. the juvenile, vegetative and reproductive phases. A particular emphasis was put on agronomical outputs (biomass and grain yield) and crop growth stresses (deficit and excess of water, thermal and nutrient stresses).Using these outputs as selection criteria, a novel multi-criteria approach was designed to retro-select the specific climatic conditions allowing to reach certain outcomes (e.g. yield target) while simultaneously exhibiting given thresholds of stresses for any considered crop stages. [less ▲]

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See detailÉdition et étude de Gerard du Frattre, compilation épique du XVIe siècle
Lambert, Adélaïde ULiege

Poster (2017, May 23)

Présentation du projet de thèse de doctorat.

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See detailInnate signaling by mycolate esters of the cell wall of Mycobacterium tuberculosis and relevance for the development of adjuvants for subunit vaccines
Tima, Giresse ULiege

Doctoral thesis (2017)

Tuberculosis remains among the most deadly health threats to humankind. This povertyrelated disease, caused by bacteria of the Mycobacterium tuberculosis complex, kills each year more than 1.5 million ... [more ▼]

Tuberculosis remains among the most deadly health threats to humankind. This povertyrelated disease, caused by bacteria of the Mycobacterium tuberculosis complex, kills each year more than 1.5 million people. It is estimated that a further 2 billion individuals are latently infected with M. tuberculosis. Most of them will never develop any clinical symptoms, although 5 to 10% of these latently infected individuals are at risk to develop TB in their lifetime. During the last decades the situation worsened because of deteriorating socioeconomic conditions, the increased incidence of drug-resistant M.tuberculosis strains and the co-infection with HIV (a major risk factor for development of TB). Currently only one vaccine is available against TB, the Bacille of Calmette and Guérin (BCG). However, its efficacy is extremely variable against the contagious form of TB - pulmonary TB - in adults and adolescents (ranging from 0 to 80%). Therefore, to control and eliminate TB, a better vaccine, efficient drug treatments and more rapid and cheaper diagnostic techniques are needed. In this PhD thesis we have attempted to provide additional information to reach this goal. Indeed, we investigated the inflammatory and adjuvant potential of mycolate esters homologous of those found in the cell wall of M. tuberculosis. These mycolate esters vary in terms of sugar (trehalose, glucose and arabinose) and lipid (alpha-, methoxy-, keto-, wax-ester MAs) moieties. TDM, TMM, GMM and AraMM were found to be potent activators of BMDCs in vitro. This activation was shown to be dependent on the Mincle pathway. Our results demonstrated that the classes of MAs bound to sugar do not consistently impact the level of pro-inflammatory and adjuvant responses induced. Rather, the nature of the sugar and the number of acyl chains bound to it seem to dictate the affinity of the glycolipid for Mincle receptor in vitro. In vivo, we observed that TDM, TMM and GMM induce similar immune responses characterized by a production of antigen-specific IFN-γ and IL-17A. The immune responses induced by AraMM are dependent on the type of formulation. Indeed, AraMM in emulsion does not induce substantial level of Th1 while AraMM in DDA liposome induces Th1 immune response. This study increases our knowledge on host-pathogen interactions during M. tuberculosis infection by identifying the receptor involved in the recognition of several PAMPs. Furthermore, we showed that the activation of this receptor by these mycolate esters triggers the induction of protective Th1 and Th17 immune responses [less ▲]

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See detailEffects of preference and sensory modality on behavioral reaction in patients with disorders of consciousness
Heine, Lizette ULiege; Tillmann, Barbara; Hauet, Marjolaine et al

in Brain Injury (2017)

Background: Reliable evaluation of patients with unresponsive wakefulness syndrome (UWS) or in a minimally conscious state (MCS) remains a major challenge. It has been suggested that the expression of ... [more ▼]

Background: Reliable evaluation of patients with unresponsive wakefulness syndrome (UWS) or in a minimally conscious state (MCS) remains a major challenge. It has been suggested that the expression of residual cerebral function could be improved by allowing patients to listen to their favourite music. However, the potential effect of music on behavioural responsiveness, as well as the effect of preferred stimuli in other sensory modalities (e.g. olfaction), remain poorly understood. Objective: The aim of our study was to investigate the effect of sensory modality (auditory versus olfactory) and preference (preferred versus neutral) of the test stimuli on patients’ subsequent performance on the Coma Recovery Scale-Revised (CRS-R). Research design: Within-subject design because of inter-individual differences between patients. Methods and procedures: We studied four items from the CRS-R (visual pursuit using a mirror, auditory localization of the own name and two movements to command) in 13 patients (7 MCS; 6 UWS). Main outcomes and results: Auditory stimuli triggered higher responsiveness compared to olfactory stimuli, and preferred stimuli were followed by higher scores than did neutral stimuli. Conclusions: Findings suggest that preferred auditory stimuli at the bedside contribute to the expression of residual function and could improve the diagnostic assessment. [less ▲]

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See detailEssential oil of Tunisian plant: biopesticide applied in agricultural system
Ben Kaab, Sofiène ULiege; ksouri, riadh; Jijakli, Haissam ULiege

Conference (2017, May 23)

The use of plant-derived products in postharvest disease management may be a valid alternative to conventional chemical treatments (Pane et al., 2016). Unfavorable environmental conditions (such as salt ... [more ▼]

The use of plant-derived products in postharvest disease management may be a valid alternative to conventional chemical treatments (Pane et al., 2016). Unfavorable environmental conditions (such as salt and drought) increase production and accumulation of reactive oxygen species (ROS). Consequently, Tunisian plants have developed adaptive responses including the synthesis of specific bioactive molecules used for medical and nutritional purposes (Ksouri et al., 2012). In that context, the main objective of the present study was the identification of essential oils from Tunisian plants against the important plant pathogens, particularly in Europe. The study began with the selection of endemic medicinal plant suspected to present antimicrobial properties. The essential oil was extracted by hydrodistillation using a Clevenger type apparatus (with a yield of 1.2%). The chemical composition of the essential oil obtained by hydro-distillation from the aerial parts was analyzed by GC/MS. Therefore, antifungal activity was evaluated against Fusarium culmorum, Fusarium oxysporum and Penicillium italicum, using ELISA microplates with a blocked randomized design, as described previously (Kouassi et al., 2012). In addition, the essential oil was then tested for their herbicidal activities in pre-emergence and post-emergence assays against three weeds species. The chromatographic analysis showed a complex mixture where twenty compounds were identified accounting for 98.75 % of the total oil. Monoterpenes was represented by 71.73 %. The dominant monoterpenes are α Pinene (7.09%), 1,8-Cineole (54.6%) and Camphor (12.27%). Essential oil reduced significantly spores germination in a dose-dependent manner. Their fungistatic activity reached 100% at 0.6% against Fusarium oxysporum . In the dose response bioassay, the essential oil reduced seed germination rate of Phalaris minor, Sylibum marianum and Trifolium incanatum. Seedling growth was measured by shoot and root lengths at day 7. At 0.5%, essential oil reduced 100% seed germination. Post-emergence bioassays consisted in spraying essential oil at 3 concentrations (0.75, 2 and 3.4 %) at 2 leaves stage of three weeds species. Pelargonic acid was used as commercial positive control at 3.4%. At 0.75% and 2%, the essential oil do not show any sign of injury. However, only at 3.4 %, the spraying of essential oil showed visible injury ranging from wilting (after 1 day) and chlorosis (after 3 day) on Trifolium incanatum and Phalaris minor. After formulation, to enhance the distribution, the coverage and the penetration of the active molecules, the essential oil presented a high herbicidal activity. In conclusion, this work allowed to open new perspectives on the application of Tunisian essential oil as Novel biocontrol strategies against damageable plant pathogens and weeds. [less ▲]

Detailed reference viewed: 63 (18 ULiège)