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See detailL'exploitation laitière de demain : conclusions
Beckers, Yves ULg

Conference (2015, February 11)

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See detailFrom scientific expertise to lived experiences of genetic diseases. On patients’ organisations’ involvement in the construction of the medical evidence
Duysens, Fanny ULg

Conference (2015, February 11)

Genomic medicine is currently an expanding field. Based on the complete sequencing of the human genome, recent innovations promote the development of new protocols of diagnostic and treatments against ... [more ▼]

Genomic medicine is currently an expanding field. Based on the complete sequencing of the human genome, recent innovations promote the development of new protocols of diagnostic and treatments against hereditary genetic diseases. Thereby, they create opportunities which can disrupt the relationships between involved actors, such as patients and their relatives, concerned organisations, medical experts, as well as genomics specialists and health policy managers. This presentation exposes the baseline questions of a research project interested in some Belgian patients’ organisations’ involvement in issues related to genetic diseases within this current context, especially in the construction of the so-called "scientific and medical evidence". It addresses its expansion to patients' experiential knowledge which is based on their lived experiences of health and disease. [less ▲]

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See detailHDAC5 Depletion in Cancer Cells Induces an Oxidative Stress and Leads to a Metabolic Reprogramming toward Glucose and Glutamine Metabolism
Hendrick, Elodie ULg; Peixoto, Paul ULg; Polese, Catherine ULg et al

Poster (2015, February 11)

Histone deacetylases (HDAC) is a family of eighteen enzymes, which modulates the acetylation level of histones and non-histone proteins to regulate gene expression and chromatin structure. Broad-spectrum ... [more ▼]

Histone deacetylases (HDAC) is a family of eighteen enzymes, which modulates the acetylation level of histones and non-histone proteins to regulate gene expression and chromatin structure. Broad-spectrum inhibitors of these enzymes such as SAHA can inhibit tumor growth both in vitro and in vivo and are currently used as anti-cancer agents in clinic. For many years, we are investigating the specific role of individual HDAC members in cancer biology and we have recently demonstrated that specific depletion of HDAC5 using siRNA technology reduced cancer cells proliferation and survival1 The goal of this study is to further understand the molecular mechanisms of action of HDAC5 in cancer cells. Screening transcriptomic study demonstrated that HDAC5 depletion induces a down-regulation of subunits of the complex I of the mitochondrial respiratory chain (NDUFB5-NDUFA3) as well as anti-oxydant proteins (Ferritin, Metalothionein,¿) through modulation of mRNA stability. Therefore, HDAC5 depletion causes a significant increase of ROS production inducing both apoptosis and mechanisms of mitochondria quality control (mitophagy and mitobiogenesis). This HDAC5 depletion-induced mitochondrial dysfunction provokes metabolic adaptation associated with increased importance of glucose and glutamine. Indeed, interference with both glucose and glutamine supply in HDAC5-depleted cancer cells significantly increases apoptotic cell death suggesting that glucose or glutamine deprivation might be combined to HDAC5 inhibition as a therapeutic strategy to kill cancer cells. Our study demonstrated for the first time that specific HDAC5 inhibition induces metabolic reprogramming and provides insight into a valuable experimental strategy for manipulation of specific HDAC5 inhibition and glucose metabolism in therapy against cancer. 1.Peixoto, P. et al. HDAC5 is required for maintenance of pericentric heterochromatin, and controls cell-cycle progression and survival of human cancer cells. Cell death and differentiation, 2012; 1-14. Presenting author e-mail: elodie.hendrick@student.ulg.ac.be [less ▲]

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See detail“Problem Practices” in EU Competition Law
Petit, Nicolas ULg

Scientific conference (2015, February 10)

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See detailTen years of Martian nitric oxide nightglow observations
Stiepen, Arnaud ULg; Gérard, Jean-Claude ULg; Gagné, Marie-Eve et al

in Geophysical Research Letters (2015)

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See detailDark anti atoms can explain DAMA
Wallemacq, Quentin ULg; Cudell, Jean-René ULg

in Journal of Cosmology and Astroparticle Physics [= JCAP] (2015)

We show that the existence of a sub-dominant form of dark matter, made of dark 'antiatoms' of a mass of 1 TeV and a size of 30 fm, can explain the results of direct detection experiments, with a positive ... [more ▼]

We show that the existence of a sub-dominant form of dark matter, made of dark 'antiatoms' of a mass of 1 TeV and a size of 30 fm, can explain the results of direct detection experiments, with a positive signal in DAMA/NaI and DAMA/LIBRA and no signal in other experiments. The signal comes from the binding of the dark anti-atoms to thallium, a dopant in DAMA, and is not present for the constituent atoms of other experiments. The dark anti-atoms are made of two particles oppositely charged under a dark U(1) symmetry and can bind to terrestrial atoms because of a kinetic mixing between the photon and the massless dark photon, such that the dark particles acquire an electric milli-charge of ± 5.10(−)(4)e. This millicharge enables them to bind to high-Z atoms via radiative capture, after they thermalize in terrestrial matter through elastic collisions. [less ▲]

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See detailC'est la faute à l'Europe !
Grandjean, Geoffrey ULg

Conference given outside the academic context (2015)

Nombreux sont les dirigeants politiques et les citoyens qui considèrent que l’Union européenne est responsable de la situation politique et économique actuelle. L’exposé vise à interroger ces discours et ... [more ▼]

Nombreux sont les dirigeants politiques et les citoyens qui considèrent que l’Union européenne est responsable de la situation politique et économique actuelle. L’exposé vise à interroger ces discours et à comprendre la place et le rôle des institutions européennes. Il permet surtout de relativiser ces discours en ciblant précisément les marges de manœuvre de l’Union européenne. [less ▲]

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See detailCognitive remediation of autobiographical memory in schizophrenia and its relation with depressive mood.
Blairy, Sylvie ULg

Scientific conference (2015, February 09)

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See detailCritique des données statistiques 'macro' disponibles dans les institutions internationales
Ozer, Pierre ULg

Scientific conference (2015, February 09)

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See detailQuestion d'actualité en environnement : un cours intégrateur/ transversal qui fait sens pour les étudiants de Bac1 en sciences Bio-ingénieur
Colaux, Catherine ULg

Scientific conference (2015, February 09)

Lors de son entrée à l'université l'étudiant primant est souvent décontenancé. Tous les changements auxquels il doit faire face (organisation des cours, exigences des enseignants, nouveau contexte social ... [more ▼]

Lors de son entrée à l'université l'étudiant primant est souvent décontenancé. Tous les changements auxquels il doit faire face (organisation des cours, exigences des enseignants, nouveau contexte social etc.) le conduisent quelques fois à douter, allant même quelques fois jusqu'à remettre en question son choix d'orientation. Les étudiants Bioingénieurs de Gembloux Agro Bio Tech ne dérogent pas à cette règle. Il nous paraissait donc primordial de proposer à l'horaire de première année un cours intégrateur qui puisse conforter les étudiants dans leur choix de filière mais plus encore, qui puisse impacter leur perception de valeur, souvent négative, de certains de leurs enseignements. Un des objectifs de ce cours est donc de démontrer à nos étudiants, en traitant un sujet d'actualité en environnement que leurs cours de mathématique, physique, chimie etc. sont nécessaires à leur formation de bioingénieurs. Ce cours se veut également innovant puisqu'il sortira du canevas ex-cathedra classiquement appliqué en première année et proposera aux étudiants un travail en petits groupes et essentiellement en ligne. Ce sera l'occasion d'aborder avec eux en tout début de cursus ces compétences transversales qui prennent de plus en plus de place dans nos formations. [less ▲]

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See detailImaging the mechanisms involved in abdominal aortic aneurysms rupture; a step towards patient-specific risk assessment
NCHIMI LONGANG, Alain ULg

Doctoral thesis (2015)

Introduction The general context of this dissertation is to evaluate patient-specifi c approaches to the risk of rupture of abdominal aortic aneurysm (AAA), using imaging techniques with ability to assess ... [more ▼]

Introduction The general context of this dissertation is to evaluate patient-specifi c approaches to the risk of rupture of abdominal aortic aneurysm (AAA), using imaging techniques with ability to assess biological processes. Following a thorough description of available imaging techniques, our work is divided in two main research objectives, namely: (i) to provide greater clinical value to existing but unproven imaging concepts, and (ii) to suggest new concepts for improved AAA risk of rupture assessment. Methods The fi rst research objective evaluated how far imaging biological activities using 18F-Fluorodeoxyglucose (FDG) Positron Emission Tomography (PET) and modeling wall stress using fi nite element simulations (FES) may help clinical decisionmaking in patients with AAA, and what would be their incremental value as compared to diameter-based patient management algorithms. On a patient basis, clinical outcomes were evaluated with regard to FDG PET and FES signaling. Further, the concept of AAA risk-equivalent diameter using FES was described and retrospectively validated using data from large multicenter trials. The second research objective included the assessment of the biological activities of the intraluminal thrombus (ILT) and the demonstration of its deleterious role in AAA using multimodality imaging. A special emphasis was put on the ability of magnetic resonance imaging (MRI) to monitor the biological activities of ILT without exogenous contrast, by evaluating its iron content. Results Increased FDG uptake was a diameter-independent marker of AAA-related events over 2 years. Missing dichotomy prevented such a fi nding for increased wall stress, although its correlation with increased FDG uptake indicates a potentially comparable value in terms of risk management. Wall metabolism is infl uenced by patient-specifi c susceptibility factors, indicating hereditary or acquired alteration of the biological responses to wall stress. The concept of risk-equivalent diameters on FES links biomechanical estimates to basic conclusions drawn from large diameter-based clinical AAA trials. Our retrospective and diameter-adjusted validation analysis verifi ed that biomechanical risk indicators are higher in ruptured than non-ruptured AAAs. Part of the FDG uptake is associated with biological activity along the luminal surface of the ILT, where we experimentally demonstrated phagocytosis of superparamagnetic iron oxide on MRI , both ex vivo and in vivo. This phagocytosis is correlated with the abundance of leukocytes and proteolytic activity. In addition, unenhanced MRI appearances resulting from the endogenous iron distribution within ILT also relate to these biological activities. Lastly, multimodality imaging was used to confi rm the concept of the deleterious role of the ILT in AAA growth in a model of AAA by infusion of elastase in the rat. Conclusion MRI and FDG PET are capable of evidencing and quantifying in vivo some of the notoriously deleterious biological processes taking place in the aneurysmal sac, especially related to the entrapped phagocytes and red blood cells in ILT and the periadventitial infl ammatory response. The central role played by ILT and its biological activities was demonstrated in vivo using several imaging techniques. The clinical value of imaging these biological activities is epitomized by a diameterindependent 2-year increased risk of event in AAA with increased wall metabolism. [less ▲]

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See detailEtude de la reconstitution immunitaire après greffe de cellules souches hématopoïétiques : focus sur la fonction thymique et les lymphocytes T régulateurs
HANNON, Muriel ULg

Doctoral thesis (2015)

L’allogre e de cellules souches hématopoïétiques, après conditionnement myéloalbatif ou non, est devenue une option de choix dans le traitement des maladies hématologiques malignes ou génétiques ... [more ▼]

L’allogre e de cellules souches hématopoïétiques, après conditionnement myéloalbatif ou non, est devenue une option de choix dans le traitement des maladies hématologiques malignes ou génétiques. Malheureusement le succès de ce traitement se trouve trop souvent entaché par des complications majeures telles que les infections ou la maladie du greffon contre l’hôte (GVHD), dans laquelle les cellules du donneur attaquent l’organisme du receveur. La reconstitution d’un système immunitaire fonctionnel, notamment via le thymus, est une étape clef dans la résolution de ces deux complications et l’utilisation de lymphocytes T régulateurs semble très prometteuse dans la lutte contre la GVHD. Le présent travail vise donc à étudier la reconstitution du système immunitaire après allogreffe sous trois angles différents, en nous focalisant sur la fonction thymique et l’influence des lymphocytes T régulateurs (Treg). Dans la première partie, nous évaluons la reconstitution immunitaire à long terme après greffe non-myéloablative et montrons que la néo-génération de lymphocytes T par le thymus s’effectue à partir du jour 100 chez les patients de moins de 60 ans alors qu’au delà de cet âge, elle est quasiment absente. Nous établissons également que la GVHD chronique (cGVHD) sévère possède des effets extrêmement délétères sur le thymus. Étant donné l’impact de la cGVHD sur le thymus et la reconstitution immunitaire, nous avons voulu comparer l’impact, sur ces deux paramètres, d’un conditionnement nonmyéloalbatif censé réduire la GVHD et composé d’une irradiation lymphoïde totale et d’anticorps anti-thymocytes (TLI-ATG) par rapport au conditionnement classique consistant en une irradiation corporelle totale associée à de la fludarabine (TBI-Flu). Il ressort de cette étude que, bien que réduisant de manière significative l’incidence de cGVHD, le conditionnement TLI-ATG altère fortement la reconstitution du système immunitaire et impacte négativement le taux de rechute/progression chez les patients traités. Enfin, toujours dans le but de réduire le taux de GVHD post-greffe, nous avons choisi d’examiner l’impact des Treg comme traitement de la xGVHD. Dans ce but, nous montrons qu’une sélection de Treg humains, sur CliniMACS par déplétion CD8/19 et sélection CD25, fournit une population de cellules enrichies en Treg capable de retarder de manière statistiquement significative l’apparition d’une GVHD xénogénique induite par injection de PBMC humains (autologues aux Treg) dans un modèle pré-clinique chez la souris NSG. De plus, lorsque injectée sans les PBMC, cette population enrichie n’induit pas par elle-même de GHVD. [less ▲]

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See detailHighly efficient, long life, reusable and robust photosynthetic hybrid core-shell beads for the sustainable production of high value compounds
Desmet, Jonathan; Meunier, Christophe; Danloy, Emeric et al

in Journal of Colloid & Interface Science (2015), 448

An efficient one-step process to synthesize highly porous (Ca-alginate–SiO2–polycation) shell: (Na-alginate– SiO2) core hybrid beads for cell encapsulation, yielding a reusable long-life ... [more ▼]

An efficient one-step process to synthesize highly porous (Ca-alginate–SiO2–polycation) shell: (Na-alginate– SiO2) core hybrid beads for cell encapsulation, yielding a reusable long-life photosynthetically active material for a sustainable manufacture of high-value metabolites is presented. Bead formation is based on crosslinking of an alginate biopolymer and mineralisation of silicic acid in combination with a coacervation process between a polycation and the silica sol, forming a semi-permeable external membrane. The excellent mechanical strength and durability of the monodispersed beads and the control of their porosity and textural properties is achieved by tailoring the silica and alginate loading, polycation concentration and incubation time during coacervation. This process has led to the formation of a remarkably robust hybrid material that confers exceptional protection to live cells against sheer stresses and contamination in a diverse range of applications. Dunaliella tertiolecta encapsulated within this hybrid core–shell system display high photosynthetic activity over a long duration (>1 year). This sustainable biotechnology could find use in high value chemical harvests and biofuel cells to photosynthetic solar cells (energy transformation, electricity production, water splitting technologies). Furthermore the material can be engineered into various forms from spheres to variable thickness films, broadening its potential applications. 2015 Elsevier Inc. All rights reserved. [less ▲]

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See detailSome misunderstood or unknown LaTeX tricks (XVI)
Merciadri, Luca ULg

E-print/Working paper (2015)

This is the second (and last) part of the tricks I found on StackExchange. First part began in the preceding issue. We will begin by a personal trick, and end by some inquiry about my LaTeX Tips & Tricks.

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See detailSome misunderstood or unknown LaTeX tricks (XV)
Merciadri, Luca ULg

E-print/Working paper (2015)

After having looked at about 150 StackExchange pages of 50 topics (that sums up to about 7500 topics’ names), I give you, in this article and in the next issue, the most interesting topics’ answers to my ... [more ▼]

After having looked at about 150 StackExchange pages of 50 topics (that sums up to about 7500 topics’ names), I give you, in this article and in the next issue, the most interesting topics’ answers to my eyes. We will first begin by two additions in regard to 31(3), 32(1). [less ▲]

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See detailSynthesis and anticancer activities of a novel class of mono- and di-metallic Pt(II)(salicylaldiminato)- (DMSO or Picolino)Cl complexes
Faiz-Ur Rahman; Ali, Amjad ULg; Rong Guo et al

in Dalton Transactions (2015)

A series of novel Pt(II) complexes [cis- and trans-Pt(II)(salicylaldimine)(DMSO)Cl (C-1), trans-Pt(II)(salicylaldimine)( 4-picoline)Cl (C-2), Pt(II)(salicylaldimine)Cl (C-3), trans- and cis/trans-Pt2(II ... [more ▼]

A series of novel Pt(II) complexes [cis- and trans-Pt(II)(salicylaldimine)(DMSO)Cl (C-1), trans-Pt(II)(salicylaldimine)( 4-picoline)Cl (C-2), Pt(II)(salicylaldimine)Cl (C-3), trans- and cis/trans-Pt2(II)(salicylaldimine) (DMSO)2Cl2 (C-4), trans-Pt2(II)(salicylaldimine)(4-picoline)2Cl2 (C-5) was synthesized and characterized. The structures of C-1-cis, C-1-trans and C-3 were determined using a single crystal X-ray analysis. This class of Pt(II) complexes has been studied for their in vitro cytotoxicity in multiple human cancer cell lines, including breast (MCF-7), liver (HepG2), lung (A549), colon (HCT116) and cervical (Hela) cancers. C-1- trans, C-2 and C-4-trans showed significant cytotoxicity to these cancer cells comparable to cisplatin. A time- and dose-dependent MTT assay revealed that these complexes can suppress cell viability and cell proliferation. Mechanistically these complexes induced pro-apoptotic gene expression such as BAX, PUMA and NOXA and thereby enhanced apoptosis. Moreover, PARP cleavage in a dose-dependent manner indicated their cytotoxic effect against cancer cells. Apoptosis of cancer cells occurred through apoptotic pathways as explained by the cytometry analysis. The DNA unwinding properties of these active Pt(II) complexes were studied by gel electrophoresis using pBR322 plasmid DNA as a target. Changes in the morphology of cancer cells were also observed upon the addition of C-1-trans, C-2 and C-4-trans. [less ▲]

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See detailZones humides : entre modifications environnementales et actions humaines
Ozer, Pierre ULg

Conference (2015, February 08)

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See detailMethionine-derived Schiff base as selective fluorescent “turn-on”chemosensor for Zn2+in aqueous medium and its application in livingcells imaging
Faiz-Ur-Rahman; Ali, Amjad ULg; Rong Guo et al

in Sensors and Actuators B: Chemical (2015)

tWe describe the quantitative synthesis of a Schiff base derived from methionine and salicylaldehyde(SMSB) which displayed turn-on selective fluorescent chemo-sensing properties for Zn2+in bufferedCH3CN ... [more ▼]

tWe describe the quantitative synthesis of a Schiff base derived from methionine and salicylaldehyde(SMSB) which displayed turn-on selective fluorescent chemo-sensing properties for Zn2+in bufferedCH3CN/H2O (1:1) solution. In physiological pH (7.2 buffered) SMSB exhibited high selectivity and sen-sitivity toward Zn2+over the other selected metal ions. Upon the addition of Zn2+33 folds of emissionenhancement was observed in SMSB and limit of detection (CL) for Zn2+was found to be 5.0 × 10−7M. Thisbinding phenomenon was studied by1H NMR and HR-MS analysis. Mass spectrometry analysis revealedthat SMSB interacts with Zn2+and form a 1:1 complex, also confirmed by Job’s plot. This probe wascompletely retrieved by adding EDTANa2solution, exhibited a fluorescent quenching effect by removingZn2+from the sensor. SMSB was observed to have a good tolerance for fluorescence detection of Zn2+in pH range 6–10. Furthermore it is successfully investigated for living cells imaging using MCF-7 cells.Combine experimental results indicated SMSB to be a useful and powerful chemosensing probe for Zn2+in water as well as in living cells. [less ▲]

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See detailQuand science politique et droit dialoguent : quelle typologie des instruments mémoriels en fonction du degré de contrainte ?
Grandjean, Geoffrey ULg

Conference (2015, February 07)

Envisager les études sur la mémoire comme sous-champ de la science politique francophone ouvre des pistes enrichissantes pour les politologues, notamment lorsqu’ils sont amenés à analyser les instruments ... [more ▼]

Envisager les études sur la mémoire comme sous-champ de la science politique francophone ouvre des pistes enrichissantes pour les politologues, notamment lorsqu’ils sont amenés à analyser les instruments mémoriels régissant un ou plusieurs système(s) politique(s). Dans cette perspective, la communication vise à présenter, d’un point de vue épistémologique, les apports découlant de l’interaction entre les sciences politique et juridique dans le processus de consolidation des études sur la mémoire comme sous-champ disciplinaire de la science politique. Plus précisément, la communication vise à offrir une typologie des instruments mémoriels en fonction du degré de contrainte et d’imposition d’une mémoire officielle. Par exemple, une résolution adoptée par une assemblée législative est faiblement contraignante alors qu’une loi réprimant le négationnisme impose à la fois une mémoire officielle tout en sanctionnant pénalement les citoyens qui ne la respectent pas. La typologie passe en revue les multiples instruments mémoriels pouvant être adoptés par les autorités publiques : résolution, loi déclarative, loi pénale, plan de commémorations ou encore mise en valeur d’un lieu de mémoire. L’accent est mis sur les instruments mémoriels de deux pays francophones : la Belgique et la France. Au final, cette communication permet d’insister sur l’apport de la science politique en analysant l’exercice de prérogatives de puissance publique et sur l’apport de la science juridique en classifiant les types d’instruments mémoriels en fonction du degré de contrainte ; tout en faisant dialoguer des cas d’étude différents. [less ▲]

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See detailThe return of Turkey to the Western Balkans: a challenge or an opportunity for the European Union ?
Lika, Liridon ULg

Conference (2015, February 07)

In the new Turkish doctrine, besides the great interests in Central Asia, Middle East, Africa and the Caucasus, the Balkans are also important. Turkey is making great efforts to play a greater role on the ... [more ▼]

In the new Turkish doctrine, besides the great interests in Central Asia, Middle East, Africa and the Caucasus, the Balkans are also important. Turkey is making great efforts to play a greater role on the international scene and creates its area of interest, especially where the links of Ottoman legacy can help. Although it is defined as a peaceful power in the region, some describe it as neo-ottoman, a term rejected by the Turkish authorities because of its possible imperialist connotation. This paper aims precisely to study the return of Turkey to the Balkans. Our central question is: what is the purpose of the Turkish foreign policy in the Western Balkans? Related issues will be addressed such as: is there a competition between Turkey and the European Union in this area? What are the specifics of Turkish foreign policy? In order to explore these research questions, this study focuses on the concept of soft power through which Turkey extends its power. Indeed, the Turkish government imposes itself on the Balkans not by force but with the help of economics, diplomacy, trade, culture and religion. Following the crisis in the EU, causing the collapse of Western investment and a relative decrease of attraction for the Balkans, Turkey has taken advantage of the situation in order to increase its influence in all areas. This contribution will proceed in three stages. Firstly, we will make an analysis of the authors of soft power to think about the character of the new Turkish geopolitical doctrine. Secondly, we are going to apply this theoretical framework to the case study identified, namely the influence of Turkey in the Western Balkan countries. Finally, we will show if exists a competition or a cooperation between Turkey and European Union in the Western Balkans. [less ▲]

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