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See detailProduction of word stress by French speaking CLIL and non-CLIL learners of Dutch
Hiligsmann, Philippe; Rasier, Laurent ULg; Degrave, Pauline

Conference (2016, September)

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See detailIdentification of bovine and porcine colistin-resistant mcr1-positive Escherichia coli.
Mainil, Jacques ULg; Muylaert, Adeline ULg; Saulmont, Marc et al

Conference (2016, September)

OBJECTIVE Polymyxins, especially colistin, have been used for years in veterinary medicine and were rediscovered a few years ago as last resort antibiotics in human medicine against multi-resistant Gram ... [more ▼]

OBJECTIVE Polymyxins, especially colistin, have been used for years in veterinary medicine and were rediscovered a few years ago as last resort antibiotics in human medicine against multi-resistant Gram negative bacterial pathogens. For years, only chromosome-mediated resistance to colistin was identified as a consequence of mutation(s) in lipid A-encoding genes. Recently, however, a plasmid-located gene (mcr1) was identified in Gram-negative enterobacteria and has since been found by PCR in several, but not all, bovine, human, porcine and poultry colistin-resistant Escherichia coli (Liu YY et al. Lancet Infect Dis, 2016, 16(2), 161-168; Nordmann P and Poirel L. Clin Microbiol Infect, 2016, 22, 398-400 ; Schwarz S and Johnson AP. J Antimicrob Chemother, 2016, in press, doi: 10.1093/jac/dkw274). The purpose of this study was to compare phenotypic and genetic for the detection of resistance to colistin and of the mcr1 gene in a collection of Escherichia coli isolated from different animal species and from humans. METHODS More than 3000 E. coli isolates from cattle, pigs, dogs, cats, horses, rabbits, chickens ducks and humans were tested for resistance to colistin by growing them on agar plates with 1g/ml of colistin. The Minimal Inhibitory Concentrations (MIC) of and the presence of the mcr1 gene in all growing isolates were determined using the E test® and colony hybridization assay with a mcr1 specific gene probe, respectively. The probe-positive isolates were further tested with the mcr1 gene specific PCR. RESULTS A total of 410 E. coli isolated grew on 1g/ml colistin-containing agar plates. The majority of isolates grew well, but several grew sparsely with only few isolated colonies. As determined by the E test®, MIC of 273 isolates (67%) was 1g/ml of colistin and higher; conversely, MIC of 137 isolates (33%) was lower than 1g/ml of colistin. Of those 410 E. coli isolates, 34 from pigs and bovines (9% of isolates growing on colistin-containing agar plates; 25% of isolates with MIC higher than 1g/ml) hybridized with the mcr1 gene-derived probe: 5 from pigs and 11 from bovines gave black spots (including five from the same calf), while 18 from pigs and one from bovine gave grey spots. All but one pig isolate had a MIC between 1.5 and 16 g/ml of colistin. Fifteen “black spot” probe-positive isolates tested positive with the mcr1 gene specific PCR as did 3 porcine “grey spot” probe-positive isolates, while the remaining 16 isolates repeatedly tested negative even after lowering the annealing temperature. CONCLUSION This study confirms that (i) the results of phenotypic assays for the detection of colistin resistance can not be always trusted; (ii) the mcr1 gene is not the only one mechanism of resistance to colistin; (iii) mcr1 variants may exist that can not be detected by the classical PCR. Phenotypic assays like growth on colistin-containing agar plates can still represent a first base screening assay, although the MIC determination using the E test® confirms a >1g/ml MIC for only 2 out of 3 growing isolates. Presence of mcr1 gene and putative variants (like the most recently described mcr2 gene; Xavier BB et al., Eurosurveillance, 21, 7 July 2016) in all probe-positive isolates will be confirmed after Whole Genome Sequencing that will also allow comparing the mcr1-positive plasmids and isolates from pigs and cattle to similar human E. coli isolates. Further studies should also be performed to identify the colistin resistance mechanism in mec-negative isolates. [less ▲]

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See detailQuelles perspectives d'avenir pour le Cifen ?
Simons, Germain ULg

Article for general public (2016)

Dans le cadre de la table ronde portant sur les 20 ans du Cifen (28 août 2015), le président actuel du Centre, G. Simons, a évoqué quelques perspectives pour le centre.

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See detailNonlinear vibration analysis of the SmallSat spacecraft: From identification to design
Detroux, Thibaut ULg; Noël, Jean-Philippe ULg; Masset, Luc ULg et al

Conference (2016, September)

The objective of the present contribution is to introduce nonlinear experimental and numerical tools and methods applicable to real-life structures. The study is illustrated using the SmallSat spacecraft ... [more ▼]

The objective of the present contribution is to introduce nonlinear experimental and numerical tools and methods applicable to real-life structures. The study is illustrated using the SmallSat spacecraft developed by Airbus Defence and Space, which possesses several localized nonlinearities. The computation of nonlinear normal modes and bifurcations reveals that the satellite possesses complex dynamics including modal interactions, quasiperiodic oscillations and isolated resonances. [less ▲]

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See detailIsolation of bacteriophages against Klebsiella pneumoniae and in vivo activity
Thiry, Damien ULg; Passet, Virginie; Dufour, Nicolas et al

Poster (2016, September)

Klebsiella pneumoniae is a bacterial pathogen able to induce severe healthcare-associated or community-acquired infections in humans and animals. The constant emergence of antibiotic resistant strains ... [more ▼]

Klebsiella pneumoniae is a bacterial pathogen able to induce severe healthcare-associated or community-acquired infections in humans and animals. The constant emergence of antibiotic resistant strains reinforces the need to find alternatives to antibiotic treatments. The use of bacteriophages is a promising approach. The aim of this study was to isolate bacteriophages directed against K. pneumoniae strains and to test their efficacy in a murine model. Bacteriophages against five different K. pneumoniae (2 of capsular type K1 and K2 and 1 undetermined) were isolated and purified from waste water collected in Paris area. The morphology of plaques (zones of bacterial killing) was recorded and several of them were purified three times by successive replating. Phage titers were determined by serial dilutions on their respective hosts as well as on 18 other Klebsiella strains to identify their host range. Kinetics of bacterial lysis were monitored during 15h at 3 multiplicities of infection, in triplicates. For in vivo experiment, a total of 10 mice were inoculated with 200 µl of K. pneumoniae (4.6E+07 CFU) by oral gavage and the level of K. pneumoniae in fecal samples was monitored for 10 days. Five mice did not receive any treatment and 5 other mice received a cocktail of three bacteriophages (8E+07 PFU) at day 4 post-inoculation. A total of 54 bacteriophages were isolated and purified with titers ranging from 2E+5 to 3.6E+10 PFU/ml. The host range study showed that bacteriophages against K. pneumoniae have a specificity related to the capsular type of their bacterial host. Lysis kinetics of bacteria suggested that different phages were isolated. Despite difficulties with the murine intestinal model, evidence was obtained that bacteriophages are able to reduce intestinal carriage. Our results show that bacteriophages isolated against K. pneumoniae are specific for a given capsular type, although further studies are necessary to provide more details on this capsular specificity and its molecular determinants. To fully address the in vivo potential of phages, a reliable mouse model of intestinal carriage of K. pneumoniae strains needs to be established. [less ▲]

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See detailIdentification of bovine methicillin resistant staphylococci from Europe, Africa and North America by colony hybridization, PCR and antibiotic sensitivity.
Ngassam Tchamba, Cyrille ULg; Thiry, Damien ULg; Bardiau, Marjorie et al

Conference (2016, September)

Mastitis is the costliest pathology in dairy cattle and staphylococci are the most prevalent bacterial mastitis pathogens worldwide. Antimicrobial treatment of mastitis has led to the selection of ... [more ▼]

Mastitis is the costliest pathology in dairy cattle and staphylococci are the most prevalent bacterial mastitis pathogens worldwide. Antimicrobial treatment of mastitis has led to the selection of resistant staphylococci, of which the Methicillin Resistant S. aureus (MRSA) are the most studied ones. Still, MR has also been described for non-aureus staphylococci (MRS) species. Bovine MRS(A) represent not only a problem in the treatment of mastitis, but also a potential hazard in public health via the inter-Staphylococcus transferability of the mobile “Staphylococcal Cassette Chromosome” (SCC) carrying the mec genes encoding MR and the zoonotic potential of some Staphylococcus species. The aim of this study is the comparison of genetic and phenotypic methods for the identification of MRS(A) isolated from bovine mastitis in European, African and North-American countries. A total of 1168 mastitis-associated staphylococci were isolated between 2005 and 2014 in Belgium, Italy, Switzerland, Senegal, Niger and Canada, and kept at -80°C until further use. Out of them, 867 isolates were identified to S. aureus while 301 isolates were non aureus staphylococci. All 1168 staphylococci were tested genetically by the dot blot hybridization assay on positively charged nylon membranes (Roche) after DNA extraction with 32P-radioactively labelled probes derived from the mecA and mecC genes and phenotypically by growth on “Chrom MRSA ID®” agar plates. Isolates positive at both or either tests were further studied by PCR targeting the same two genes and by the disk diffusion assay to oxacillin and cefoxitin. A total of 265 isolates (23%) were positive at both or either tests. Out of them, 27 S. aureus (10%) but no non-aureus (0%) tested positive both for DNA hybridization with the mecA probe and for growth on “Chrom MRSA ID®” plates. No isolate tested positive with the mecC probe. In addition, 32 S. aureus (12%) and 15 non aureus (6%) were positive with the mecA probe only and 169 S. aureus (64%) and 22 non aureus (8%) grew on “Chrom MRSA ID®” plates only. The S. aureus originate from Belgium (105), Italy (6), Canada (31), Senegal (38) and Niger (48) whereas the non-aureus originate from Belgium (25), Italy (1) and Niger (11). All of them are being tested with the PCR targeting the mecA gene and by the disk diffusion assay to oxacillin and cefoxitin. Most isolates (72%) grew on “Chrom MRSA ID®” plates only while few (18%) were positive to the hybridization with the mecA probe only. This high difference between the results of both tests could be explained by the weak specificity of phenotypic tests comparing to genetic tests. The others 10% of the isolates (S. aureus) which are positive with the two methods (dot blot hybridization and “Chrom MRSA ID®”) can be considered as MRSA mediated by the mecA gene. However, results of PCR and disk diffusion assay will confirm respectively the presence of mec genes and which of the two methods is the most suitable for identifying MRS from mastitis cases in cattle. Comparison of the results of phenotypic and genetic assays will indicate whether other variant(s) than mecA and mecC may be present in MRS. Further genetic and phenotypic studies are needed to (i) identify the non-aureus isolates to the species level; (ii) compare the MRS(A) isolated in the different countries by their biotypes, serotypes, lysotypes, and virulotypes, without forgetting their SCCmec and their clonal complex; and (iii) identify the mec gene variant present in hybridization-positive PCR-negative isolates, if any. [less ▲]

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See detailDu processus aux soins intégrés: Expérience de gestion de projet bottom-up
ERPICUM, Marie ULg; BASSLEER, Bernard ULg; DEFRAIGNE, Jean ULg et al

in Gestions Hospitalieres (2016), 558(Août/septembre 2016),

Le service de chirurgie cardiaque du CHU de Liège a soutenu des recherches visant à développer un programme d’épargne sanguine, enjeu actuel de taille dans ce secteur des soins de santé. Ce projet a ... [more ▼]

Le service de chirurgie cardiaque du CHU de Liège a soutenu des recherches visant à développer un programme d’épargne sanguine, enjeu actuel de taille dans ce secteur des soins de santé. Ce projet a évolué vers la création d’un itinéraire clinique chirurgical cardiaque et d’un modèle institutionnel pour le développement d’autres itinéraires cliniques. Une évolution qui permet de déterminer les missions spécifiques de l’institution et ses objectifs stratégiques, et de s’associer aux projets nationaux. L’adhésion multidisciplinaire, soutenue par un leadership médical et infirmier, ainsi que la reconnaissance institutionnelle sont les déterminants de la pérennité de cette démarche bottom-up. [less ▲]

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See detailMonitoring topography of laboratory fluvial dike models subjected to breaching based on a laser profilometry technique
Rifai, Ismail ULg; Erpicum, Sébastien ULg; Archambeau, Pierre ULg et al

in International Symposium on River Sedimentation: Stuttgart 19th-22d september 2016 (2016, September)

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See detailUn monde sans frontières ?
Jacquemain, Marc ULg

Article for general public (2016)

En s'appuyant sur la conception de la communauté politique chez Michael Walzer, le texte tente, très brièvement, de décrire la nature de la citoyenneté dans un monde d'Etats-Nations, les droits et les ... [more ▼]

En s'appuyant sur la conception de la communauté politique chez Michael Walzer, le texte tente, très brièvement, de décrire la nature de la citoyenneté dans un monde d'Etats-Nations, les droits et les exigences qu'elle comporte ainsi que le domaine des relations de l'Etat avec les non-citoyens [less ▲]

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See detailFlocculation properties of the bacterial exopolysaccharide FucoPol
Araújo, Diana; Reis, Patricia; Marques, Anna et al

Conference (2016, September)

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See detailRadioscopy laboratory diagnostic of epizootic diseases in Belgium and European countries
Cargnel, Mickaël ULg; Roelandt, Sophie; Van der Stede, Yves et al

Poster (2016, September)

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See detailFunctional properties of extracellular polysaccharides produced by Enterobacter A47 grown on agro-food industry by-products
Antunes, Silvia; Freitas, Filomena; Alves, Vitor et al

Poster (2016, September)

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See detailAdaptation interculturelles du questionnaire "Patient-Rated Tennis Elbow Evaluation" pour les patients francophones
Kaux, Jean-François ULg; DELVAUX, François ULg; SCHAUS, Jean ULg et al

in 9ème Congrès commun SFMES - SFTS (2016, September)

Contexte: L’épicondylite latérale est une blessure commune chez les joueurs de tennis et les travailleurs physiques pour qui il existe un questionnaire en anglais qui en évalue la sévérité. Le ... [more ▼]

Contexte: L’épicondylite latérale est une blessure commune chez les joueurs de tennis et les travailleurs physiques pour qui il existe un questionnaire en anglais qui en évalue la sévérité. Le questionnaire « Patient-Rated Tennis Elbow Evaluation » (PRTEE) mesure la douleur et l’incapacité fonctionnelle du coude chez les patients souffrant d’une épicondylite latérale. Le questionnaire a déjà été traduit en suédois, en turc, en québécois, en italien, en néerlandais et en grec. Objectifs : Le but de cette étude est de traduire et de réaliser une adaptation interculturelle du questionnaire PRTEE en français et d’en évaluer sa fiabilité et sa validité. Matériel et Méthodes : Le PRTEE a été adapté interculturellement en français selon les lignes directrices internationales. Les participants (n=115) ont rempli le PRTEE-F, deux fois avec un intervalle de 30 minutes, le questionnaire Disabilities of Arm, Soulier and Hand (DASH), et le Short Form Health Survey (SF-36) pour évaluer la fiabilité et la validité du PRTEE-F. La cohérence interne (avec l’alpha de Cronbach), la fidélité test-retest (avec la corrélation intra-classe (ICC)) et la validité de construit (avec le coefficient de corrélation de Spearman). Résultats : Le PRTEE-F montre une fidélité test-retest bonne pour le score total (ICC 0,83) et pour les différents items (ICC 0,71-0,9). La traduction française possède une cohérence interne élevée (0,98). La corrélation entre le PRTEE-F et le DASH est forte pour le score total (rs=0,92, p<0.001), pour le sous-total symptomatique (rs=0,86, p<0.001) et fonctionnel (rs=0,93, p<0.001). Le PRTEE-F possède une corrélation forte avec une partie des sous-échelles convergentes du SF-36 (PF, RP et BP). Il y a une corrélation faible ou modérée avec les sous-échelles divergentes du SF-36 (REm, MH, SF et VT). Il n’y a pas d’effet plancher et plafond. Conclusion : La version française du questionnaire PRTEE a été adaptée interculturellement avec succès, et cette étude a montré que le PRTEE-F est fiable et valide pour évaluer les patients francophones souffrant d’une épicondylite latérale. [less ▲]

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See detailAspects de droit international privé
Wautelet, Patrick ULg

in Derème, François (Ed.) Patrimoine et oeuvres d’art - Questions choisies (2016)

Cette contribution offre une analyse de certaines questions de droit international privé qui peuvent être utiles pour le collectionneur privé d'oeuvres artistiques. La dimension internationale d'une ... [more ▼]

Cette contribution offre une analyse de certaines questions de droit international privé qui peuvent être utiles pour le collectionneur privé d'oeuvres artistiques. La dimension internationale d'une collection fait en effet peser certains risques et offre des opportunités aux parties. Pour ce faire, lle texte aborde d'abord la constitution d'une collection, thème qui est illustré en prenant appui sur le régime international du contrat d'achat (section 1). Dans un deuxième temps, ce sont les questions de transmission qui ont retenu l'attention. La transmission peut avoir lieu du vivant du collectionneur ou après son décès. Ces deux temps sont explorés successivement (section 2 et section 3) 1. La présente contribution portera exclusivement sur les aspects civils de la gestion et de la transmission d'une collection d'oeuvres d'art, à l'exclusion des questions fiscales, en ce compris celles relatives au paiement de droits de succession par le biais d'une dation. [less ▲]

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See detailThe added value of new GNSS to monitor the ionosphere
Warnant, René ULg; Deprez, Cécile ULg; Van de Vyvere, Laura

Conference (2016, September)

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See detailLes Nouveaux Liégeois. Migrations et Transformations Urbaines
Lafleur, Jean-Michel ULg; Stangherlin, Gregor

in Dérivations (2016), (3), 194-203

Dans cet article, nous proposons de discuter la migration contemporaine sur le territoire de la Ville de Liège. Cette discussion repose pour l’essentiel sur une analyse des données statistiques ... [more ▼]

Dans cet article, nous proposons de discuter la migration contemporaine sur le territoire de la Ville de Liège. Cette discussion repose pour l’essentiel sur une analyse des données statistiques disponibles et une revue de la littérature scientifique produite sur les migrations à Liège ces dernières années. Nous commençons donc par une description des grandes évolutions démographiques sur le territoire de la ville depuis 1991 pour insister ensuite sur les effets de la migration dans cinq quartiers de la ville. Cette première partie souligne le rôle essentiel que joue la migration dans la croissance démographique observée ces dix dernières années à Liège. Ensuite, nous examinons la présence étrangère sur le territoire de la ville selon les 5 dimensions fréquemment citées dans les débats sur l’intégration des populations étrangères : emploi, logement, éducation, socio-culturelle et politique. [less ▲]

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See detailSuccessions internationales : du bon usage de l’autonomie de la volonté
Wautelet, Patrick ULg

in Pintens, Walter; Declerck, Charlotte (Eds.) Patrimonium 2016, (2016)

Cette contribution entend donner des conseils et fournir des précisions sur l'utilisation de l'autonomie de la volonté sous l'empire du Règlement 6502/2012. Le texte aborde successivement la clause d ... [more ▼]

Cette contribution entend donner des conseils et fournir des précisions sur l'utilisation de l'autonomie de la volonté sous l'empire du Règlement 6502/2012. Le texte aborde successivement la clause d'élection de for et la clause de choix de loi. Pour chacune de ces clauses, le texte propose d'abord une synthèse des possibilités offertes par le Règlement, avant de proposer des pistes pour une rédaction efficace des dispositions testamentaires ou autres. [less ▲]

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See detailHousing Quality as Environmental Inequality: The Case of Wallonia, Belgium
Lejeune, Zoé ULg; Xhignesse, Guillaume ULg; Kryvobokov, Marko et al

in Journal of Housing and the Built Environment (2016), 31(3), 495-512

First in the USA and then in many other countries, scholarship on environmental inequality has sought to shed light on the unequal environmental conditions borne by poor people and ethnic minorities, and ... [more ▼]

First in the USA and then in many other countries, scholarship on environmental inequality has sought to shed light on the unequal environmental conditions borne by poor people and ethnic minorities, and to challenge public policies and their unjust impacts on those target groups. Housing quality, especially the indoor characteristics of homes, offers an innovative perspective in this field of research. In previous research on environmental inequality in the Walloon context, housing quality has been proven to be a major determinant of quality of life and environmental well-being. This paper analyses housing quality through a twofold approach: through indoor characteristics on the one hand, and outdoor subjective and objective externalities on the other. It reveals the disparities between the most deprived and the wealthiest segments of the population. The evidence for this study is based on a housing quality survey carried out in 2012 and 2013 on 6,018 households in Wallonia (Belgium). The key findings are that poor people are found to live in housing of lower quality, in densely populated neighbourhoods and those with mixed use, with compensating amenities provided at the local level. Moreover, consistent with environmental inequality scholarship, deprived households are found to bear the burden of environmental degradation outside the home. People live in areas with poorer air quality, but are found to benefit from greater access to green spaces. The results of the survey reveal an interesting point concerning the environmental inequality literature; the interior features of housing are found to differ more widely between deprived and wealthier people than the surrounding environment does. [less ▲]

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