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See detailL'évolution du droit dans le temps et dans l'espace
Gerkens, Jean-François ULg

Scientific conference (2014, October 06)

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Peer Reviewed
See detailPotential Proteomic Biomarkers Associated To Mucosal Healing In Crohn’s Disease
MEUWIS, Marie-Alice ULg; Baiwir, Dominique ULg; Mazzucchelli, Gabriel ULg et al

Poster (2014, October 06)

Introduction and objectives: In Crohn's disease (CD), there is a discrepancy between clinical activity of the disease (symptoms) and intestinal healing. However absence of tissue healing is associated ... [more ▼]

Introduction and objectives: In Crohn's disease (CD), there is a discrepancy between clinical activity of the disease (symptoms) and intestinal healing. However absence of tissue healing is associated with the risk of relapse and tissue damage progression. Endoscopy is costly and invasive. Hence biomarkers correlating with intestinal healing could improve disease management. We aimed to identify potential biomarkers associated to CD mucosal healing by a shotgun proteomics label free study. Methods: We used the STORI clinical trial cohort (n=103) aiming at identifying markers associated to relapse prediction after Infliximab treatment withdrawals. We used serum samples of patients in clinical remission, grouped according to the degree of intestinal healing seen at endoscopy. We performed depletion of the 20 most abundant plasma proteins on each serum pools and ran a proteomics label free differential analysis using 2D-nanoUPLC-MSE HDMS Synapt G1 for data acquisition and Protein Lynks Global Server vs 2.4 for data analysis (Waters, Corp., Milford, USA). Results and Discussion: We obtained potential biomarkers and designed a multiplexed -selected reaction monitoring (SRM) method for validation of these candidates in each individual patient. The method may also be tested in an independent set of IBD patients with and without mucosal healing. Conclusions: This research strategy and results of SRM validation of potential biomarkers associated to mucosal healing in this cohort of CD patients as well as the tests done on other CD patients, may provide new opportunities for CD follow-up tests development. [less ▲]

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See detailHuman cortical excitability depends on time spent awake and circadian phase
Ly, Julien ULg; Gaggioni, Giulia ULg; Chellappa, Sarah Laxhmi ULg et al

Scientific conference (2014, October 04)

At any point in time, human performance results from the interaction of two main factors: a circadian signal varying with the time of the day and the sleep need accrued throughout the preceding waking ... [more ▼]

At any point in time, human performance results from the interaction of two main factors: a circadian signal varying with the time of the day and the sleep need accrued throughout the preceding waking period. But what’s happen at the cortical cerebral level? We used a novel technique coupling transcranial magnetic stimulation with electroencephalography (TMS/EEG) to assess the influence of time spent awake and circadian phasis on human cortical excitability. Twenty-two healthy young men underwent 8 TMS/EEG sessions during a 28 hour sleep deprivation protocole. We found that cortical excitability depends on both time spent awake and circadian phasis. [less ▲]

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See detailDevelopment and optimization of pegylated lipoplexes for vaginal application
Lechanteur, Anna ULg; Furst, Tania ULg; Kanber, Erdem et al

Conference (2014, October 04)

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Peer Reviewed
See detailCognitive rehabilitation : Past, present and future.
Majerus, Steve ULg

Conference (2014, October 04)

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See detailNEAR-DEATH EXPERIENCES IN PATIENTS WITH LOCKED-IN SYNDROME
Charland-Verville, Vanessa ULg; Lugo Ramirez, Zulay del Rosario ULg; Jourdan, Jean-Pierre et al

Poster (2014, October 04)

Near-Death Experiences (NDEs) are classically associated with positive emotions like peacefulness, happiness and joy [1-3]. To date, few negative NDEs reports have been documented [4]. Although NDEs ... [more ▼]

Near-Death Experiences (NDEs) are classically associated with positive emotions like peacefulness, happiness and joy [1-3]. To date, few negative NDEs reports have been documented [4]. Although NDEs classically arise in the context of an acute severe brain damage, their associated memories are reported as being phenomenologically very rich and detailed [5]. To date, no satisfactory explanatory model exits to fully account for the rich phenomenology of NDEs following a severe acute brain injury [6]. Neurobiological hypotheses include cerebral hypoxia [7, 8] and temporal lobe dysfunctions [9] to account for some of the features occurring during NDEs. However, it has been recently shown that anoxic/hypoxic, traumatic and other supratentorial brain lesions do not seem to influence the content of a NDE when assessed with a standardized tool (i.e., Greyson NDE scale; [1]). Due to their particular brain lesion (i.e., pontine brainstem), locked-in syndrome (LIS) patients provide a unique opportunity to further investigate the neural correlates of NDEs. We here aimed at retrospectively characterizing the content of NDEs in patients with LIS having suffered from an acute brainstem lesion (cerebrovascular accident (CVA) or trauma; i.e., “LIS NDEs”) and to compare these experiences to those collected in a cohort of matched NDE experiencers after coma with supratentorial lesions (CVA or trauma; i.e., “classical NDEs”). [less ▲]

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See detailNeuropathies proximales du membre supérieur
WANG, François-Charles ULg

Conference (2014, October 04)

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See detailL'épaule un carrefour complexe
WANG, François-Charles ULg

Conference (2014, October 04)

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See detailEt si ce n'était pas un canal carpien
FERON, Frédéric ULg; WANG, François-Charles ULg

Conference (2014, October 04)

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See detailDiscussion de cas cliniques et atelier pratique
WANG, François-Charles ULg

Conference (2014, October 04)

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Peer Reviewed
See detailOblitérations. Contacts et frictions dans "A Humument" de Tom Phillips
Delville, Michel ULg; Belloi, Livio ULg

Conference (2014, October 03)

Cette intervention examine "A Humument" de Tom Phillips en termes de contacts et de frictions entre le texte-source (un roman victorien) et sa réinterprétation par l'auteur.

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See detailWhat's happening in Adenoma Valley
Beckers, Albert ULg

Scientific conference (2014, October 03)

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See detailMultibody Modelling of Mechanical Transmission Systems in Vehicle Dynamics
Virlez, Geoffrey ULg

Doctoral thesis (2014)

This thesis addresses the dynamic modelling of automotive driveline components by means of the nonlinear finite element method for flexible multibody systems. Starting from a detailed study of an ... [more ▼]

This thesis addresses the dynamic modelling of automotive driveline components by means of the nonlinear finite element method for flexible multibody systems. Starting from a detailed study of an industrial application, the TORSEN differential, several contributions are proposed in the field of contact modelling. The contact between rigid bodies in several geometric configurations is investigated and the influence of the squeeze film of lubricating oil is studied. A contact formulation between flexible bodies modelled as superelements is also developed and applied to the modelling of 3D flexible gear pairs. [less ▲]

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See detailDétection précoce de la souffrance psychique chez l’adolescent
Malchair, Alain ULg

Scientific conference (2014, October 03)

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See detailFunction of the chloroplastic hydrogenase in the microalga Chlamydomonas reinhardtii: A trvel from dark to light
Godaux, Damien ULg

Doctoral thesis (2014)

The decreasing availability of fossil energy stocks and the eventuality of tragic climate changes caused by greenhouse gases lead to search for alternative renewable energy sources. Biological hydrogen ... [more ▼]

The decreasing availability of fossil energy stocks and the eventuality of tragic climate changes caused by greenhouse gases lead to search for alternative renewable energy sources. Biological hydrogen might be one promising renewable energy carrier. A specific and restricted group of microalgae developed the ability to produce hydrogen based on an oxygen-sensitive hydrogenase enzyme coupled to the photosynthetic pathway, acting as a putative valve for excess electrons in conditions where other electron acceptors are scarce. The unicellular green alga Chlamydomonas reinhardtii is widely regarded as a model organism for various biological processes, especially for photosynthesis. Moreover, the capacity of Chlamydomonas hydrogenase is claimed as the highest recorded in literature. Less than twenty years ago, a group of American scientists designed a new approach for sustained photobiological production of hydrogen, based on a two-stage protocol that temporally separates photosynthetic O2 evolution from the H2 production phase (Melis et al., 2000). The transition occurs upon sulfur deprivation of the culture and leads to an operating continuous production for several days, opening new possibilities in the aim of an economically rentable bioproduction. For these reasons, hydrogen photoproduction in Chlamydomonas reinhardtii has been extensively examined in the last decade as extension of photosynthesis research entailing the understanding of hydrogen metabolism in microalgae (for reviews, see Hankamer et al., 2007; Ghirardi et al., 2009; Ghysels and Franck, 2010). Despite the attractive trait of generating a renewable fuel from nature’s most plentiful resources, i.e. light and water, the physiological significance of such oxygen-sensitive enzyme coupled to oxygenic photosynthesis has been poorly investigated with the exception of some old studies (Kessler, 1973; Schreiber and Vidaver, 1974). In this work, hydrogenase implication in photosynthetic reactivation from dark and anoxic environment is investigated. In the first part of the work, by analyzing several strains affected in hydrogen metabolism (e.g. nda2-RNAi (Jans et al., 2008), pfl1 (Philipps et al., 2011), dum11 (Dorthu et al., 1992)), we show that the PSII–dependent photosynthetic electron flow upon dark to light shift is linearly related to the activity of hydrogenase, both for short and long-terms adaptation (Publication I). In agreement with this conclusion, a hydrogenase-deficient strain for the HydEF maturation factor (hydef, Posewitz et al., 2004) shows peculiar chlorophyll fluorescence induction kinetics after adaptation to dark and anoxia. Based on these findings, a novel imaging screening method is developed, allowing rapid identification of strains impaired in hydrogen metabolism. Compared to existing screens (for review, see Hemschemeier et al., 2009), our protocol is remarkably fast, sensitive and non-invasive. At this stage, application of this new screening method allowed us to isolate several hydrogenase-deficient strains, among which one was impaired for the hydrogenase maturation protein HydG (hydg-2 mutant). Chlamydomonas reinhardtii might frequently encounter period of dark and anoxia in its natural habitat, especially during the night when the microbial community respires the available oxygen. In the second part of my work, the physiological importance of hydrogenase is investigated in the context of photosynthesis induction at the onset of light upon anoxia. In such conditions, the plastoquinone pool is known as being overreduced. This triggers the process of state transitions which is described as allowing the redistribution of light capture between both photosystems to manage the redox poise of the photosynthetic pathway (for review, see Lemeille and Rochaix, 2010). We therefore revisit the impact of both state transitions and hydrogenase activity on the reactivation of photosynthetic electron flow (Publication II). Here we show that, in presence of hydrogenase, photosynthesis reactivation is slightly faster in stt7 mutant locked in state 1 (Depege et al., 2003) compared to wild type which is in state 2. However, photosynthesis reactivation is delayed in hydef stt7-9 double mutant compared to hydef mutant. This indicates that, in a hydrogenase-deficient context, state 2 promotes photosynthesis reactivation. Considered for a long time as being tightly interconnected (Finazzi et al., 1999; Finazzi et al., 2002; Finazzi and Forti, 2004), state transitions and PSI-CEF have recently been revealed as unrelated to each other (Takahashi et al., 2013). Nonetheless, the increasing of PSI antenna size in state 2 could even though enhance the PSI-CEF rate, in an indirect way, by enhancing PSI energy capture (Cardol et al., 2009; Alric, 2014). This reasonably raises the question of a possible involvement of PSI-CEF in photosynthesis induction. This possibility is further studied in the third and last part of the work. Thanks to mutants devoid of PSI-CEF (i.e. pgrl1 mutant (Tolleter et al., 2011)) and hydrogenase activity (i.e. hydg-2 mutant (Publication I)), we investigate the role played by PSI-CEF along with hydrogenase during photosynthesis reactivation during a shift from dark anoxia to light (Publication III). Herein, we demonstrate that Calvin cycle reactivation is proton gradient-dependent, most likely due to ATP requirement for carbon dioxide fixation. By measuring the PSI/PSII efficiency ratio during the re- illumination period, we point out the physiological occurrence of PSI-CEF within the first minutes of ilumination. We therefore propose a schematic model that assesses the electron flow through hydrogenase, PSI-CEF and Calvin cycle in function of the illumination period in all studied strains. Although lack of PSI-CEF does not appear to be essential for cell survival, photosynthesis reactivation is delayed in pgrl1 mutants. We also isolate a pgrl1 hydg-2 double mutant and demonstrate that the combination of both defects prevents any photosynthetic activity and strongly impairs growth. This highlights the importance for algae to keep both pathways in the course of evolution, being critical for the survival of Chlamydomonas reinhardtii in its natural environment. [less ▲]

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