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See detailDifferent and synergistic effect of 5-Azacytidine and Rapamycin on ex vivo expansion of natural human T Regulatory cells
Conteduca, Giuseppina ULg

Poster (2016, October 14)

Natural T regulatory cells (Treg) are challenging to expand ex vivo, and this has severely hindered in vivo evaluation of their therapeutic potential. 5-Azacytidine (5-azaC) and Rapamycin (RAPA) are ... [more ▼]

Natural T regulatory cells (Treg) are challenging to expand ex vivo, and this has severely hindered in vivo evaluation of their therapeutic potential. 5-Azacytidine (5-azaC) and Rapamycin (RAPA) are immunosuppressive drugs that promote selectively the expansion of CD4+CD25highFoxp3+ regulatory T cells.: We investigated whether 5-azaC and RAPA could be used together to promote the ex vivo expansion of Tregs purified from adult human peripheral blood.We found that 5-azaC helped maintain FOXP3 expression during the expansion process probably by promoting the conversion of T conventional (Tconv) in Treg, instead Rapa induces selectively apoptosis in Tconv cells and expansion in Treg. Addition of 5-azaC to RAPA treated cultures improved gene expression of FOXP3, CD25, STAT5 and TGF-B resulted in enhanced Treg expansion and suppressive activity. Also Rapa and 5-AzaC combination sustain Bcl-2 protein expression in Treg conferring resistance to apoptosis process. 5-azaC may have utility in ex vivo expansion of human Tregs, not as a single agent, but in combination with RAPA. These data may considerably accelerate the development of immunotherapeutic approaches for the treatment of autoimmune disease or posttransplant alloreactions by the adoptive transfer of nTreg cells [less ▲]

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See detailBouger à tout âge? Oui, mais …
Cloes, Marc ULg

Conference given outside the academic context (2016)

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See detailInfluence of short-term synoptic events and snow depth on DMS, DMSP, and DMSO dynamics in Antarctic spring sea ice
Carnat, Gauthier; Brabant, Frédéric; Dumont, Isabelle et al

in Elementa: Science of the Anthropocene (2016), 4(0), 000135

Temporal changes in the concentration profiles of dimethylsulfide (DMS), dimethylsulfoniopropionate (DMSP), and dimethylsulfoxide (DMSO) were measured in pack ice from the Bellingshausen Sea (Antarctica ... [more ▼]

Temporal changes in the concentration profiles of dimethylsulfide (DMS), dimethylsulfoniopropionate (DMSP), and dimethylsulfoxide (DMSO) were measured in pack ice from the Bellingshausen Sea (Antarctica) during the winter-spring transition of 2007. Two sites with contrasting snow and ice thicknesses were sampled, with high concentrations of DMS, DMSP, and DMSO observed at both sites, especially in surface ice. These high concentrations were shown to correspond to the development of a surface ice microalgal community dominated by strong DMSP producers (flagellates and dinoflagellates) following flooding of the ice cover. Several short-term synoptic events were observed and shown to influence strongly the dynamics of sea ice DMS, DMSP, and DMSO. In particular, a cold spell event was associated with drastic changes in the environmental conditions for the sea ice microbial communities and to a remarkable increase in the production of dimethylated sulfur compounds at both sites. A good correlation between all dimethylated sulfur compounds, sea ice temperature, and brine salinity suggested that the observed increase was triggered mainly by increased thermal and osmotic stresses on microalgal cells. Atmospheric forcing, by controlling sea ice temperature and hence the connectivity and instability of the brine network, was also shown to constrain the transfer of dimethylated sulfur compounds in the ice towards the ocean via brine drainage. Analysis of the two contrasting sampling sites shed light on the key role played by the snow cover in the sea ice DMS cycle. Thicker snow cover, by insulating the underlying sea ice, reduced the amplitude of environmental changes associated with the cold spell, leading to a weaker physiological response and DMS, DMSP, and DMSO production. Thicker snow also hampered the development of steep gradients in sea ice temperature and brine salinity, thereby decreasing the potential for the release of dimethylated sulfur compounds to the ocean via brine drainage. [less ▲]

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See detailCase report autour de cristalluries médicamenteuses atypiques
Castiglione, Vincent ULg; GADISSEUR, Romy ULg

Conference (2016, October 14)

We report here the cases of uncommon-shaped crystals in patient's urine. The first one is a 73years-old man with massive crystalluria observed in his microscopic urinalysis on Sedimax° (Menarini ... [more ▼]

We report here the cases of uncommon-shaped crystals in patient's urine. The first one is a 73years-old man with massive crystalluria observed in his microscopic urinalysis on Sedimax° (Menarini). Observed crystals had various shape (needle, stick, Feather...). The patient was hospitalized because of lactic acidosis due to suicide attempt by medication overdose. The following medication had been taken: prazepam, acetaminophen, tramadol and Triumeq°, a new anti-retroviral association of dolutegravir, abacavir and lamivudine. Infrared analysis of dryed urine didn"t allow to identify any common stone component, nor any medication. We further discuss about the possible implication of dolutegravir in crystalluria in this patient. Secondly, we show several N-acetylsulfamethoxazol (sulfamethoxazol metabolite) urine crystals detected in microscopic urinalysis of various patients. All had uncommon shape and were sometimes associated to acute kidney injury [less ▲]

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See detailRepeat-sales price indices for the US corporate bond market: Index quality and asset pricing tests
Beaupain, Renaud; Heck, Stéphanie ULg

Scientific conference (2016, October 14)

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See detailExploring Metacognition as Support for Learning Transfer
Devlin, Marie; Draeger, John; Dvorakova, Lucie Sam et al

Conference (2016, October 14)

This presentation will share our efforts to investigate how metacognition might enhance the transfer of learning from one context to another. This topic is of relevance because the ability to transfer ... [more ▼]

This presentation will share our efforts to investigate how metacognition might enhance the transfer of learning from one context to another. This topic is of relevance because the ability to transfer one’s learning to new situations lies at the heart of lifelong learning and the employability of university graduates. [less ▲]

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See detailTreg, Th17 and γδ T cells during normal and abortive pregnancy
Polese, Barbara ULg; Gridelet, Virginie ULg; Munaut, Carine ULg et al

Conference (2016, October 14)

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See detailHépatite C. Dépister largement...et guérir!
DELWAIDE, Jean ULg

in Le Journal du Médecin (2016), (2467), 20-21

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See detailThe Terra Mosana Project
Billen, Roland ULg

Conference (2016, October 14)

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See detailIntegrated continuous flow photoreactors: Photooxidation of (L)-methionine with singlet oxygen
Emmanuel, Noémie ULg; Mendoza Gallego, Carlos ULg; Lismont, Marjorie ULg et al

Poster (2016, October 13)

Development of photochemistry in macroscopic batch reaction vessels is hampered due to inherent limitations: light penetration remains superficial, resulting in inhomogeneous irradiation and hence to side ... [more ▼]

Development of photochemistry in macroscopic batch reaction vessels is hampered due to inherent limitations: light penetration remains superficial, resulting in inhomogeneous irradiation and hence to side-reactions or product degradation due to overexposure. The recent implementation of photochemical processes in microreactors under continuous-flow conditions appeared to be much more powerful than its batch analogue in terms of irradiation efficiency and light penetration. Furthermore, the fine control of residence time ensures an accurate control of the irradiation time, avoiding side-reactions and degradation. The photooxidation of (L)-methionine with singlet oxygen using Rose Bengal as a sensitizer was successfully implemented in a microreactor setup and led to total and selective conversion into methionine sulfoxide, an important building block for the organic synthesis of peptides or functionalized amino acid. The reaction was performed in 20 min while the same reaction in batch took 2h. [less ▲]

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See detailAccuracy of dichotomous logistic IRT models to estimate ability with multiple choice items
Magis, David ULg

Conference (2016, October 13)

This presentation focuse son multiple-choice items without any control or correction for guessing. Most often these items are recoded as binary outcomes (TRUE-FALSE) and logistic IRT models are calibrated ... [more ▼]

This presentation focuse son multiple-choice items without any control or correction for guessing. Most often these items are recoded as binary outcomes (TRUE-FALSE) and logistic IRT models are calibrated using these recoded data. The main purpose of this study is to highlight the limits of this approach by (a) proposing a simulation model to allow for guessing at various levels of ability, and (b) comparing the dichotomous approach to the use of nominal response model. Preliminary simulations indicate that none of the models are very accurate to estimate latent abilities accurately, though the nominal response model has fewer bias then the dichotomous models. [less ▲]

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See detailLeaving Gauss: extending Gaussian variance bounds to arbitrary targets
Clette, Emilie ULg; Swan, Yvik ULg

Conference (2016, October 13)

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See detailA Safe and Reliable Continuous-Flow System towards Methylphenidate Hydrochloride
Gerardy, Romaric ULg; Monbaliu, Jean-Christophe ULg

Poster (2016, October 13)

The batch-to-flow translation of a carbene-based strategy for the diastereoselective preparation of the active pharmaceutical ingredient threo-methylphenidate hydrochloride is presented. The continuous ... [more ▼]

The batch-to-flow translation of a carbene-based strategy for the diastereoselective preparation of the active pharmaceutical ingredient threo-methylphenidate hydrochloride is presented. The continuous-flow system implies the generation of an explosive organic azide, its consumption in a diazo transfer reaction, an intermediate purification step followed by a Rh(II)-catalyzed carbene C-H insertion reaction that produces threo-enriched N-Boc-methylphenidate. Boc-deprotection is carried out off-line and provides methylphenidate hydrochloride in 19% isolated yield (3 steps fully telescoped) or 38% isolated yield (sequential). [less ▲]

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See detailCurating Canons of Comics: Daniel Clowes and Art Spiegelman's 'Private' Museums
Crucifix, Benoît ULg

Conference (2016, October 13)

As guest of honor at the 2012 International Comics Festival in Angoulême, Art Spiegelman did not only have a retrospective exhibition devoted to his work, but was also invited to act as the curator of the ... [more ▼]

As guest of honor at the 2012 International Comics Festival in Angoulême, Art Spiegelman did not only have a retrospective exhibition devoted to his work, but was also invited to act as the curator of the CIBDI museum’s archive, producing his own ‘private’ museum, as suggested by the exhibition title Le Musée privée d’Art Spiegelman. Similarly, for Daniel Clowes’s retrospective exhibit in Columbus, the Wexner Center of the Arts hosted Eye of the Cartoonist: Daniel Clowes’s Selections from Comics History, based on Clowes’s curatorial choices from the Billy Ireland Cartoon Library and Museum. In each case, the cartoonist literally frames the history of comics and exhibits his personal canon of comics. As Spiegelman put it: “I have been allowed to highjack the Centre of Bande dessinée Museum to replace what is primarily the Francophone patrimony of comics with my own perverse and private map of what comics are.” This quote clearly embodies the dual facet of such exhibitions – collective and singular, shared and idiosyncratic – foregrounding the anchorage of individual graphic novelists within traditions of comics. Articulated around personal canons, these two exhibitions offer fresh perspectives to think about canonization in the comics world. Albeit focused on two 'canonized' graphic novelists, this case study nuances the auteurism of comics scholarship: not only are these exhibitions the result of collaborative work, they also recast the author in the role of curator. The key aspect of these exhibitions lies in a canonizing act of selection that circulates in varied material forms showcasing the authors’ influences. Mapping out how Clowes and Spiegelman frame their entanglement with the past of comics within the contexts of institutions that store its archive, I will examine the specificities of these exhibitions in relation to a body and style of historiography I call ‘authors’ histories of the comics.’ [less ▲]

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See detailImplication of AurA kinase in GBM cells chemotaxis in response to the production of CXCL12 in the subventricular zones
Willems, Estelle ULg; Dedobbeleer, Matthias ULg; LOMBARD, Arnaud ULg et al

Poster (2016, October 13)

Despite great improvement in standard therapies (i.e. surgery, radiotherapy and chemotherapy) of glioblastoma (GBM), the median survival rate is 15 months due to patient relapses. A major advance in the ... [more ▼]

Despite great improvement in standard therapies (i.e. surgery, radiotherapy and chemotherapy) of glioblastoma (GBM), the median survival rate is 15 months due to patient relapses. A major advance in the understanding of GBM recurrences has been the identification of GBM-initiating cells (GIC). GIC are thought to be deeply involved in GBM recurrences. Our lab designed a mouse model by grafting human GBM cells in the striatum. After the graft, we observed that tumors develop in the mouse striatum and that GIC specifically invade the subventricular zones (SVZ). SVZ are stem cells niches crucial for adult neurogenesis which seems particularly propitious for gliomagenesis since they are abundant in growth factors and permissive to proliferation. We therefore looked for soluble factors secreted by the SVZ environment and demonstrated that the local production of the CXCL12 chemokine in the SVZ is responsible for the GIC-directed migration. In this work, we aim to study the role GBM therapeutic resistance associated with the invasion of the SVZ. In this work, we identified a new actor of the CXCL12 pathway by the phosphoproteome analysis of U87MG cells stimulated with CXCL12: the mitotic kinase Aurora A (AurA) whose activity seems crucial for the CXCL12-dependent chemotaxis of GBM cells [less ▲]

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See detailDesign and synthesis of Fe2O3 nanoparticles/N-doped porous carbon structures as high performance electrode for lithium ion battery
Alkarmo, Walid ULg; Ouhib, Farid ULg; Aqil, Abdelhafid ULg et al

Poster (2016, October 13)

Thanks to their fascinating physical properties such as high surface area, multidimensional electron transport pathways and good mechanical strength, three dimensionally (3D) interconnected carbon porous ... [more ▼]

Thanks to their fascinating physical properties such as high surface area, multidimensional electron transport pathways and good mechanical strength, three dimensionally (3D) interconnected carbon porous frameworks have emerged as attractive materials for various electrochemical energy storage/conversion devices, including Li-ion batteries (LIBs), Li−S batteries, supercapacitors and fuel cells. In this context, a hierarchically structured macro- and mesoporous N-doped carbon with dispersed Fe2O3 nanoparticles (NDC@Fe2O3) is prepared by thermal treatment of a novel composite composed by PMMA particles decorated by graphene oxide (GO), PPy and iron salts. The NDC@Fe2O3 composite exhibited high surface area with a hierarchical pores structure. The combination of Fe2O3 nanoparticles with porous carbon to form hybrid anode has been an efficient way to maintain the electronic integrity of the whole electrode since the carbon acts as a buffer layer to accommodate the volume variation and to provide multidimensional electron transport pathways during the charge/discharge process. [less ▲]

Detailed reference viewed: 45 (9 ULg)