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See detailPolitical and Social Thought at the Time of the Italian Wars: France and Burgundy
Dumont, Jonathan ULg

Scientific conference (2014, December 12)

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See detailSpatial variation in the concentrations of mercury and persistent organic pollutants in free-ranging bottlenose dolphins (Tursiops truncatus) from South Florida
Damseaux, France ULg; Kiszka, Jeremy; Heithaus et al

Poster (2014, December 12)

The bottlenose dolphin (Tursiops truncatus) is an abundant apex predator found in nearshore waters of South Florida, especially in the Lower Florida Keys (Key West) and the coastal waters of Everglades ... [more ▼]

The bottlenose dolphin (Tursiops truncatus) is an abundant apex predator found in nearshore waters of South Florida, especially in the Lower Florida Keys (Key West) and the coastal waters of Everglades National Park (ENP). The objective of this study was to assess variation in contamination levels of total mercury (T-Hg) and persistent organic pollutants (NDL-PCBs, PBDEs, DDT, HCH, HCB, PCDD/Fs and DL-PCBs) in bottlenose dolphins found offshore of the densely populated Key West (n = 27) and from undeveloped ENP (n = 20). T-Hg and POPs were analysed in skin and blubber, respectively, by the mean of Direct Mercury analyser (for THg), GC-ECD (POPs) and GC-HRMS (DLCs). The 7 ICES PCBs were the main compounds found in bottlenose dolphins from Key West (8229 ng.g-1 lipids) and the ENP (2289 ng.g-1 lipids), while the concentrations of PCDD/Fs remained low (Key West: 104 pg.g-1 lipids, ENP: 102 ng.g-1 lipids). POP concentrations were higher in individuals from Key West compared to those from the ENP. However, POPs concentrations in Key West dolphins were lower than those from other locations in Florida and around the world. Unlike organic pollutants, T-Hg concentrations were significantly higher in ENP dolphins (Key West: 2941 ng.g-1 dw versus ENP: 9314 ng.g-1 dw) highlighting the specific cycle of Hg in mangrove ecosystems. To conclude, sources of T-Hg and POPs differed between Key West and ENP as reflected by their concentrations in skin and blubber of free-ranging bottlenose dolphins highlighting their role as sentinels of their environment. [less ▲]

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See detailDetection of small amounts of human adenoviruses in stools: comparison of a new immuno real-time PCR assay with classical tools.
Bonot, Sebastian; Ogorzaly, Leslie; Elmoualij, Benaïssa ULg et al

in Clinical Microbiology & Infection (2014), 20(12), 1-7

The detection of low virus concentrations in biological matrices, especially stool samples, is facing significant limitations as far as common diagnostic methods (enzyme-linked-immunosorbent assay (ELISA ... [more ▼]

The detection of low virus concentrations in biological matrices, especially stool samples, is facing significant limitations as far as common diagnostic methods (enzyme-linked-immunosorbent assay (ELISA) or quantitative real-time PCR (qPCR)) are considered. Here the development of a new immuno real-time PCR (iPCR) is described and its performance in the detection of human adenoviruses (HAdVs) in spiked stools is compared with those of ELISA and qPCR assays. For the iPCR, detection of the sandwich formed by the complexation of capture antibody-antigen-detection antibody was performed by qPCR thanks to the substitution of peroxydase by a chimeric DNA. This modification increased the detection sensitivity 200-fold compared to ELISA. The direct qPCR results revealed that only 0.3–9.5% of the spiked HAdV were detectable, resulting from important losses of DNA occurring at the extraction step. This step was not necessary in the iPCR workflow, avoiding this drawback. The losses of viral particles occurred at the elution step from the stool only. The recovery rate of the iPCR was thus better and ranged between 21 and 54%. As a result, iPCR enabled the detection of lower virus concentrations in stool samples compared to those detected by ELISA and qPCR. The iPCR could be considered as a ‘hyper sensitive ELISA’ for early detection of HAdV infections, especially in the case of immunocompromised patients after haematopoietic stem cell transplant. [less ▲]

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See detailStable isotope ratios reveal trophic niche partitioning among hermit crabs from tropical polyspecific seagrass meadows
Michel, Loïc ULg; Frederich, Bruno ULg; Lavitra, Thierry et al

Poster (2014, December 12)

Polyspecific seagrass meadows are ubiquitous features of tropical coastal zones. These ecosystems are of critical ecological importance, and provide a wide range of socio-economical services to local ... [more ▼]

Polyspecific seagrass meadows are ubiquitous features of tropical coastal zones. These ecosystems are of critical ecological importance, and provide a wide range of socio-economical services to local populations. Meadows however undergo multiple threats linked to human activities (increased nutrient input, overfishing, invertebrate overharvesting, etc.). It is currently hard to assess how seagrass meadows could respond to anthropogenic impacts due to poor knowledge of their functional ecology. In an effort to unravel trophic interactions ruling the food webs associated to seagrass beds of the Toliara Great Reef (SW Madagascar), we studied resource segregation between two common Diogenidae hermit crabs (Dardanus scutellatus and Ciliopagurus tricolor) using stable isotope ratios. Interspecific differences were noted in isotopic composition of carbon (δ13C = -12.22 ± 1.73 ‰ for D. scutellatus, δ13C = -14.55 ± 0.73 ‰ for C. tricolor), nitrogen (δ15N = 4.73 ± 0.53 ‰ for D. scutellatus, δ15N = 5.20 ± 0.61 ‰ for C. tricolor) and sulfur (δ34S = 14.08 ± 2.32 ‰ for D. scutellatus, δ34S = 16.73 ± 1.49 ‰ for C. tricolor), suggesting that the two species do not feed on the same items. In addition, SIBER (Stable Isotope Bayesian Ellipses in R) modeling based on C and N data clearly showed that no overlap was present in the core isotopic niches of the two species. It also indicated that the isotopic niche of D. scutellatus was greater than the one of C. tricolor, implying that the former feeds on a greater number of items than the latter. While hermit crabs are generally considered as omnivorous species, this study highlighted differences in the foraging ecology of D. scutellatus and C. tricolor. These differences could help to limit competition for food between these two species, and facilitate their coexistence in Malagasy seagrass beds. [less ▲]

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See detailPoésie et révolte
Saint-Amand, Denis ULg

Conference given outside the academic context (2014)

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See detailLes progrès de la foresterie sociale en Afrique centrale
Vermeulen, Cédric ULg; Doucet, Jean-Louis ULg

Conference given outside the academic context (2014)

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See detailMise au point d'une forme stable de l'allergène Der p 3 de Dermatophagoides pteronyssinus pour le diagnostic rapide et le développement de nouvelles approches d'immunothérapie de l'allergie aux acariens
Bouaziz, Ahlem ULg

Doctoral thesis (2014)

L’allergie aux acariens touche 20 à 30 % de la population occidentale. L’allergène Der p 3 de l’acarien Dermatophagoides pteronyssinus est une protéase à serine active appartenant à la famille de la ... [more ▼]

L’allergie aux acariens touche 20 à 30 % de la population occidentale. L’allergène Der p 3 de l’acarien Dermatophagoides pteronyssinus est une protéase à serine active appartenant à la famille de la trypsine. Son activité protéolytique pourrait être un facteur adjuvant impliqué dans le développement et la chronicité de l’allergie. A l’heure actuelle, l’implication de Der p 3 dans l’allergie est peu connue et son taux de fixation des IgE provenant de patients allergiques est controversé. Cependant, ces différentes études ont été réalisées au moyen d’un allergène naturel très sujet à l’autolyse et pouvant être contaminé par d’autres protéase. Durant la première partie de cette étude, nous avons pu montrer que rDer p 3 produit en P.Pastoris présente les mêmes propriétés allergéniques que nDer p 3. En effet, les résultats obtenus par test ELISA, inhibition compétitive montrent que rDer p 3 est correctement replié et présente les mêmes épitopes B conformationnels que l’allergène naturel. Dans la deuxième partie de cette thèse, nous avons pu mettre en évidence que l’autolyse de Der p 3 durant le test ELISA a un effet sur la reconnaissance des IgE. En effet, le taux mesuré avec la forme inactive est deux fois supérieur par rapport à la forme active. Der p 3S196A pourrait être donc un meilleur outil de diagnostic pour l’allergie à Der p 3. Dans la troisième partie de cette thèse, nous avons pu montrer que le propeptide de proDer p 3 affecte l’allergénicité de Der p 3. Par une étude in vitro, nous avons prouvé par ELISA, par les tests de dégranulation des basophiles de rat humanisés et d’activation des basophiles humains, que proDer p 3 est moins bien reconnu par les IgE de patients allergiques que la forme mature Der p 3. Ensuite, nous avons montré que les souris sensibilisées par les formes mature et zymogène de l’allergène Der p 3 ont développé une réponse immune de type TH2 et TH1 respectivement. ProDer p 3 pourrait donc être considéré comme une forme hypoallergénique de l’allergène Der p 3. Dans la dernière partie, nous avons construit un modèle tridimensionnel de l’allergène Der p 3 afin de prédire les épitopes B conformationnels par approche bioinformatique. En effet, 3 épitopes B ont été prédits ainsi que les acides aminés impliqués dans la reconnaissance et la fixation des IgE au sein de chaque épitope. Enfin, au moyen de la technique du peptide array, nous avons pu mettre en évidence un épitope B linéaire de l’allergène Der p 3. [less ▲]

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See detailAufmerksamkeit - eine neue Form symbolischen Kapitals (?)
Houscheid, Karin ULg

Scientific conference (2014, December 11)

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See detailProhibitive strategies and prohibitive cycles in Ancient Egyptian
Grossman, Eitan; Polis, Stéphane ULg

Scientific conference (2014, December 11)

In this talk, we present the main prohibitive/negative jussive strategies attested for each state of the language in Ancient Egyptian and we describe the grammaticalization pathways of two prohibitive ... [more ▼]

In this talk, we present the main prohibitive/negative jussive strategies attested for each state of the language in Ancient Egyptian and we describe the grammaticalization pathways of two prohibitive constructions, from Old Egyptian down to Coptic. The paper is structured as follows. In the introduction (§1), a brief review of current typological studies of prohibitives will be given as background information. Then, we start with a description of the two main types of prohibitive constructions that one finds in Coptic, taking into dialectal variety (§2), namely mpr+V(ERB) and mn-V(ERB) “do not V”. Afterwards, we describe the grammaticalization pathway along which the first of these two constructions developed, from Old Egyptian down to Coptic (§3). Additionally, we provide a description of the main prohibitive (as well as negative jussive) strategies that are attested for Earlier (§4) and Later Egyptian (§5), in order to situate more precisely the grammaticalization process of the first strategy within the successive ‘synchronic’ systems of oppositions in the semantic field of prohibition. In a final section (§6), we discuss more in depth the second, more marginal, prohibitive construction of Coptic (mn-V) — investigating Coptic dialectal diversity — and we suggest a diachronic scenario that could account for the appearance and development of this second strategy. [less ▲]

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See detailAssessing spatio-temporal variability and trends in modelled and measured Greenland Ice Sheet albedo (2000–2013)
Alexander, P.; Tedesco, M.; Fettweis, Xavier ULg et al

in Cryosphere (The) (2014), 8

Accurate measurements and simulations of Greenland Ice Sheet (GrIS) surface albedo are essential, given the role of surface albedo in modulating the amount of absorbed solar radiation and meltwater ... [more ▼]

Accurate measurements and simulations of Greenland Ice Sheet (GrIS) surface albedo are essential, given the role of surface albedo in modulating the amount of absorbed solar radiation and meltwater production. In this study, we assess the spatio-temporal variability of GrIS albedo during June, July, and August (JJA) for the period 2000–2013. We use two remote sensing products derived from data collected by the Moderate Resolution Imaging Spectroradiometer (MODIS), as well as outputs from the Modèle Atmosphérique Régionale (MAR) regional climate model (RCM) and data from in situ automatic weather stations. Our results point to an overall consistency in spatio-temporal variability between remote sensing and RCM albedo, but reveal a difference in mean albedo of up to ~0.08 between the two remote sensing products north of 70° N. At low elevations, albedo values simulated by the RCM are positively biased with respect to remote sensing products by up to ~0.1 and exhibit low variability compared with observations. We infer that these differences are the result of a positive bias in simulated bare ice albedo. MODIS albedo, RCM outputs, and in situ observations consistently indicate a decrease in albedo of −0.03 to −0.06 per decade over the period 2003–2013 for the GrIS ablation area. Nevertheless, satellite products show a decline in JJA albedo of −0.03 to −0.04 per decade for regions within the accumulation area that is not confirmed by either the model or in situ observations. These findings appear to contradict a previous study that found an agreement between in situ and MODIS trends for individual months. The results indicate a need for further evaluation of high elevation albedo trends, a reconciliation of MODIS mean albedo at high latitudes, and the importance of accurately simulating bare ice albedo in RCMs. [less ▲]

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See detailPsychopathie, adaptation et traitement du stimulus émotionnel
Grandjean, Sylvie; Englebert, Jérôme ULg

Scientific conference (2014, December 11)

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See detailLaïcité à la française : de la prétention universaliste à l'obsession identitaire
Jacquemain, Marc ULg

Conference (2014, December 11)

Le mot "laïcité" est revendiqué par des groupes d'extrême-droite ouvertement racistes et conspirationnistes comme "Riposte laïque". L'article montre que le même imaginaire conspirationniste, sous la même ... [more ▼]

Le mot "laïcité" est revendiqué par des groupes d'extrême-droite ouvertement racistes et conspirationnistes comme "Riposte laïque". L'article montre que le même imaginaire conspirationniste, sous la même structure narrative peut être retrouvé sous des formes débarrassée de leur racisme explicite dans des textes considérés comme "mainstream" au sein de la laïcité, en analysant un texte du Rappel. A partir de là, on tente de montrer que cette "dérivation conspirationniste" peut trouver des prises dans des formulations "progressistes" de la laîcité dès lors que ces formulations partent pour point de départ le point de vue à la fois holiste et idéaliste d'un "laos" toujours-dejà-là, comme chez Peña ruiz [less ▲]

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See detailCystic Fibrosis screening by simultaneous ELISA for IRT1 and PAP proteins. A pilot study.
BOEMER, François ULg

Scientific conference (2014, December 11)

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See detailImprovement of cellulose anaerobic digestion
Kinet, Romain ULg

Scientific conference (2014, December 11)

Detailed reference viewed: 17 (4 ULg)