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See detailDiscovering novel enzymes y functional screening of plurigenomic libraries from alga-associated Flavobacteriia and Gammaproteobacteria
Martin, Marjolaine ULg; Vandermies, Marie ULg; Joyeux, Coline et al

in Microbiological Research (2016), 186

Alga-associated microorganisms, in the context of their numerous interactions with the host and thecomplexity of the marine environment, are known to produce diverse hydrolytic enzymes with ... [more ▼]

Alga-associated microorganisms, in the context of their numerous interactions with the host and thecomplexity of the marine environment, are known to produce diverse hydrolytic enzymes with originalbiochemistry. We recently isolated several macroalgal-polysaccharide-degrading bacteria from the sur-face of the brown alga Ascophyllum nodosum. These active isolates belong to two classes: the Flavobacteriiaand the Gammaproteobacteria. In the present study, we constructed two “plurigenomic” (with multi-ple bacterial genomes) libraries with the 5 most interesting isolates (regarding their phylogeny andtheir enzymatic activities) of each class (Fv and Gm libraries). Both libraries were screened for diversehydrolytic activities. Five activities, out of the 48 previously identified in the natural polysaccharolyticisolates, were recovered by functional screening: a xylanase (GmXyl7), a beta-glucosidase (GmBg1), anesterase (GmEst7) and two iota-carrageenases (Fvi2.5 and Gmi1.3). We discuss here the potential role ofthe used host-cell, the average DNA insert-sizes and the used restriction enzymes on the divergent screen-ing yields obtained for both libraries and get deeper inside the “great screen anomaly”. Interestingly, thediscovered esterase probably stands for a novel family of homoserine o-acetyltransferase-like-esterases,while the two iota-carrageenases represent new members of the poorly known GH82 family (contain-ing only 19 proteins since its description in 2000). These original results demonstrate the efficiencyof our uncommon “plurigenomic” library approach and the underexplored potential of alga-associatedcultivable microbiota for the identification of novel and algal-specific enzymes. [less ▲]

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See detailLa polykystose rénale autosomique dominante : comment et pourquoi identifier les patients "rapidement progresseurs" vers l'insuffisance rénale terminale?
bodson, aurélie; MEUNIER, Paul ULg; Krzesinski, Jean-Marie ULg et al

in Revue Médicale de Liège (2016), 71(4), 184-192

Autosomal dominant polycystic kidney disease (ADPKD) is a common inherited disease characterised by the progressive development of multiple and bilateral cysts in kidneys and other organs. Most patients ... [more ▼]

Autosomal dominant polycystic kidney disease (ADPKD) is a common inherited disease characterised by the progressive development of multiple and bilateral cysts in kidneys and other organs. Most patients with ADPKD will develop, sooner or later, end-stage renal disease (ESRD). The morbidity and mortality associated with ESRD prompt physicians to identify early ADPKD patients considered as «rapid progressors», who have the greatest risk to rapidly develop ESRD. The rate of progression can be assessed by clinical - especially with the «predicting renal outcome in polycystic kidney disease score» (PROPKD-Score) -, biological (a decline of the glomerular filtration rate (GFR) of 4,4 - 5,9 ml/min/year and/or the doubling of serum creatinine within a 36-month period), or radiological criteria (total kidney volume (TKV) adjusted for the size > 600 cc/m and/or TKV annual growth rate > 5 %). Nowadays, there is no curative treatment for ADPKD. However, vasopressin-2 receptor antagonists, such as tolvaptan, appear to slow down the growth of renal cysts and the slope of GFR decline. The current management of ADPKD patients is mostly based on correcting the risk factors for progression, i.e. encouraging (over)-hydration, normalizing blood pressure, stimulating smoking cessation. [less ▲]

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See detailHigh spectral resolution monitoring of Nova V339 Delphini with TIGRE (Corrigendum)
De Gennaro Aquino, I.; Schröder, K.-P.; Mittag, M. et al

in Astronomy and Astrophysics (2016), 589

The tags in Table 2, indicating the positions of the most prominent spectral features in Figs. 2a and b, were not corrected during the revision process. We include here the corrected table. The lines ... [more ▼]

The tags in Table 2, indicating the positions of the most prominent spectral features in Figs. 2a and b, were not corrected during the revision process. We include here the corrected table. The lines observed and identified in the spectra and the respective phases remain unchanged. [less ▲]

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See detailTesting the theory of colliding winds: the periastron passage of 9 Sagittarii. I. X-ray and optical spectroscopy
Rauw, Grégor ULg; Blomme, R.; Nazé, Yaël ULg et al

in Astronomy and Astrophysics (2016), 589

Context. The long-period, highly eccentric O-star binary 9 Sgr, known for its non-thermal radio emission and its relatively bright X-ray emission, went through its periastron in 2013. <BR /> Aims: Such an ... [more ▼]

Context. The long-period, highly eccentric O-star binary 9 Sgr, known for its non-thermal radio emission and its relatively bright X-ray emission, went through its periastron in 2013. <BR /> Aims: Such an event can be used to observationally test the predictions of the theory of colliding stellar winds over a broad range of wavelengths. <BR /> Methods: We conducted a multi-wavelength monitoring campaign of 9 Sgr around the 2013 periastron. In this paper, we focus on X-ray observations and optical spectroscopy. <BR /> Results: The optical spectra allow us to revisit the orbital solution of 9 Sgr and to refine its orbital period to 9.1 years. The X-ray flux is maximum at periastron over all energy bands, but with clear differences as a function of energy. The largest variations are observed at energies above 2 keV, whilst the spectrum in the soft band (0.5-1.0 keV) remains mostly unchanged, indicating that it arises far from the collision region, in the inner winds of the individual components. The level of the hard emission at periastron clearly deviates from the 1 /r relation expected for an adiabatic wind-interaction zone, whilst this relation seems to hold at the other phases that are covered by our observations. The spectra taken at phase 0.946 reveal a clear Fe xxv line at 6.7 keV, but no such line is detected at periastron (φ = 0.000), although a simple model predicts a strong line that should be easily visible in the data. <BR /> Conclusions: The peculiarities of the X-ray spectrum of 9 Sgr could reflect the effect of radiative inhibition as well as a phase-dependent efficiency of particle acceleration on the shock properties. [less ▲]

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See detailSemiotyka wizualna między tekstualnością a praktyką. Wokół wypowiadania
Dondero, Maria Giulia ULg

in Thiel-Jańczuk, Katarzyna (Ed.) Taktyki wizualne. Michel de Certeau i obrazy (2016)

Niniejszy artykuł stawia sobie dwa cele. Pierwszym jest porównanie semiotyki tekstu Algirdasa Juliena Greimasa i Louisa Marina z semiotyką praktyk Jacques’a Fontanille’a. Prace Michela de Certeau na temat ... [more ▼]

Niniejszy artykuł stawia sobie dwa cele. Pierwszym jest porównanie semiotyki tekstu Algirdasa Juliena Greimasa i Louisa Marina z semiotyką praktyk Jacques’a Fontanille’a. Prace Michela de Certeau na temat praktyk odbioru i użycia pozwolą wyjaśnić relację między pojęciem wypowiadania wypowiedzianego (énonciation énoncée) w paradygmacie tekstu i wypowiadania w akcie (énonciation en acte) w paradygmacie praktyk. Drugim celem jest problematyzacja pojęcia wypowiadania w ramach języka wizualnego. W oparciu o teorie i analizy Louisa Marina (Opacité en peinture – Nieprzejrzystość w malarstwie) ukazana zostanie użyteczność pojęcia wypowiadania wypowiedzianego w studiach nad obrazami i podjęta zostanie próba przetestowania znaczenia, które wypowiadaniu nadaje de Certeau (Wynaleźć codzienność 1. Sztuki działania) w ramach odbioru i interpretacji obrazów. [less ▲]

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See detailPrésentation des recherches: Minus solvit qui tardius solvit
Cavalleri, Vanessa ULg

Scientific conference (2016, April)

Ma recherche doctorale vise à réaliser une étude chronologique et historique de la mora debitoris, depuis son apparition en droit romain jusqu’à nos jours. Il fallait dès lors déterminer les contours de ... [more ▼]

Ma recherche doctorale vise à réaliser une étude chronologique et historique de la mora debitoris, depuis son apparition en droit romain jusqu’à nos jours. Il fallait dès lors déterminer les contours de la mora debitoris en droit romain: ses modes de déclenchement, ses effets, son utilité et étudier l’évolution du concept lors des époques successives. Une question principale a balisé mes recherches: celle de la démarcation entre le retard du débiteur et la faute du débiteur: en effet, le droit romain a toujours été clair: à partir du moment où il est en retard, le débiteur du droit romain répond d’une responsabilité absolue (il répondra de la perte de l’objet de l’obligation qui est survenue après la mora même si l’objet disparait à cause d’un cas fortuit). Son obligation devient en effet perpétuelle: le débiteur reste tenu alors même que son obligation devient techniquement irréalisable. On constate cependant que le droit positif aborde les choses différemment: même si la règle de base reste la même (le débiteur retardataire est tenu même des cas fortuits), il est aujourd’hui possible pour le débiteur en mora de se libérer de son obligation alors même qu’il est en demeure, en prouvant que la chose aurait péri de la même manière si elle avait été remise à temps au créancier (exemples: art. 1302 C. Napoléon et art. 103 C.O.S) Le droit positif exige donc qu’il existe un lien de causalité entre le retard du débiteur et la disparition de l’objet afin que le débiteur retardataire ne soit tenu pour responsable. Cependant, malgré ce changement, la demeure persiste comme une institution propre. Les propositions d’uniformisation du droit civil au niveau européen voire même international vont cependant encore plus loin: le droit européen actuel désire unifier le droit des contrats (et plus particulièrement, le droit de la vente). Sous l'impulsion de cette démarche, on remarque que la séparation traditionnelle opérée dans les codes civils européens entre mora, impossibilité d'exécution et exécution fautive ou inexécution tend à disparaître au sein tentatives d’uniformisation du droit européen les plus récentes (parmi lesquelles le DCFR, dans lequel la notion de mora disparait pour se fondre dans celle de « non performance » (exemple: art. III. – 1:102: Definitions (3)), alors que les principes UNIDROIT de 1994 et les EPL (European Principles of Contract Law), également connus sous le nom de projet Lando (commission on european contract law, commissionnés par l’Union européenne), adoptent la voie médiane du nachtfrist, une option médiane permettant de prendre en compte le retard du débiteur : (art. 326 BGB) : le nachfrist permet de donner une extension automatique du temps imparti pour exécuter les contrats commerciaux. Bien que cette extension soit obligatoire en Allemagne, elle est laissée à la discrétion des parties dans les systèmes internationaux qui l’ont adoptée.) En définitive, il convient donc de se demander si l’évolution proposée par le droit européen est intéressante: peut-on effectivement se passer de la notion millénaire de demeure du débiteur, pourtant présente dans la plupart des Codes Civils européens. Quels avantages la mora procure-t-elle par rapport au droit de l’inexécution ? De surcroit, la sécurité juridique est-elle alors garantie par l’évolution que connait le principe ? Voici les questions qui ont jalonné mes recherches. [less ▲]

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See detailPregnancy outcome following frozen embryo transfer after artificial cycle or treatment by clomiphene citrate.
JOUAN, Caroline ULg; EMONARD, Violaine ULg; RUGGERI, Philippe ULg et al

in Gynecological Endocrinology : The Official Journal of the International Society of Gynecological Endocrinology (2016), 29

The optimal method to prepare endometrium before frozen embryo transfer (FET) is not yet established. We retrospectively studied 4496 FET and detailed pregnancy and miscarriage rates in three groups of ... [more ▼]

The optimal method to prepare endometrium before frozen embryo transfer (FET) is not yet established. We retrospectively studied 4496 FET and detailed pregnancy and miscarriage rates in three groups of patients according to the endometrium preparation they have followed before their successive FET: clomifene citrate (CC, group 1), artificial cycle (AC, group 2) or switch between CC and AC (group 3). The overall pregnancy rates per transfer were 24.3, 20.8 and 17.3% while the miscarriage rates reached 23.2, 29.8 and 42.5%, respectively. Group 1 experienced the highest ongoing pregnancy rate (18.6%), the lowest being observed in group 3 (10.0%, p50.001). Here we propose several alternatives to improve our AC protocol (group 2) that seemed less effective than CC (group 1) and we consider the use of a gonadotrophinstimulated treatment for patients with the lowest reproductive outcomes (group 3). [less ▲]

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See detailCompact handheld low-cost biosensor platform for remote health monitoring
Hastanin, Juriy ULg; Lenaerts, Cedric ULg; Gailly, Patrick ULg et al

in Proceedings of SPIE; SPIE 2016 Photonics Europe (2016, April)

In this paper, we present an original concept of plasmonic-related instrumentation platform dedicated to diagnostic biosensing tests out of the laboratory. The developed instrumental platform includes ... [more ▼]

In this paper, we present an original concept of plasmonic-related instrumentation platform dedicated to diagnostic biosensing tests out of the laboratory. The developed instrumental platform includes both disposable one-use microfluidic affinity biochip and compact optical readout device for biochip monitoring involving mobile Internet devices for data processing and communication. The biochip includes both microfluidic and optical coupling structures formed into a single plastic slab. The microfluidic path of the biochip operates in passive capillary pumping mode. In the proof-of-concept prototype, we address specifically the sensing format involving Surface Plasmon Resonance phenomenon. The biochip is plugged in the readout device without the use of an index matching fluid. An essential advantage of the developed biochip is that its implementation involves conventional hot embossing and thin film deposition process, perfectly suited for mass production of low-cost microfluidic biochip for biochemical applications. [less ▲]

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See detailDesign optimization of large-size format edge-lit light guide units
Hastanin, Juriy ULg; Lenaerts, Cedric ULg; Fleury-Frenette, Karl ULg

in Proceedings of SPIE; SPIE 2016 Photonics Europe (2016, April)

In this paper, we present an original method of dot-pattern generation dedicated to design optimization of large-size format ELBUs (Edge-Lit Light Guide Unit), where the number of dots greatly exceeds the ... [more ▼]

In this paper, we present an original method of dot-pattern generation dedicated to design optimization of large-size format ELBUs (Edge-Lit Light Guide Unit), where the number of dots greatly exceeds the maximum allowable number of optical objects supported by most common ray-tracing software. In the proposed method, in order to simplify the computational problem, an original optical system is replaced by an equivalent one. Accordingly, the original dot pattern is splitted into multiple small sections. Then, these sections are replaced by equivalent cells with continuous diffusing film. After that, we adjust the TIS (Total Integrated Scatter) two-dimensional distribution over the grid of equivalent cells, using an iterative optimization procedure. Finally, the obtained optimal TIS distribution is converted into the dot size distribution by applying an appropriate conversion rule. An original semi-empirical equation dedicated to rectangular large-size LGPs (Light Guide Plate) is proposed for the initial guess of the TIS distribution. It allows significantly reducing the time needed to dot pattern optimization. [less ▲]

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See detailLe hate speech et la liberté d'expression dans la jurisprudence américaine sous l'angle de la philosophie du langage
Thirion, Nicolas ULg; Vandenbulke, Antoine ULg

in Auteurs & Media (2016)

Analysis of landmark US Supreme Court cases on hate speech evaluated in terms of philosophy of language, particularly through the works of J.L. Austin and Judith Butler.

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See detailreactive gases in the chemistry climate model ECHAM6-HAMMOZ
Schultz, Martin; Taraborrelli, Domenico; Stadtler, Scarlet et al

Conference (2016, April)

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See detailThe Impact of Vulnerability and Resilience to Environmental Changes on Mobility Patterns in West Africa
Zickgraf, Caroline ULg; Vigil Diaz Telenti, Sara ULg; de Longueville, Florence et al

Report (2016)

From the Sahel to the coast, West Africa is experiencing a variety of climate change impacts, including sea level rise, soil salinization, floods, drought, and desertification, while simultaneously ... [more ▼]

From the Sahel to the coast, West Africa is experiencing a variety of climate change impacts, including sea level rise, soil salinization, floods, drought, and desertification, while simultaneously suffering from other forms of environmental degradation. Together, these environmental changes are significantly influencing migration patterns in and out of West Africa. This paper seeks to analyze vulnerability and resilience to environmental changes as they affect and are affected by mobility patterns in the region. We assert that the impact of environmental changes cannot be isolated from other political, social, economic, and demographic pressures that together drive human mobility. In a region where natural resources form the foundation of livelihoods and food security (fishing and agriculture), the relationship between environmental changes and socioeconomic vulnerabilities is of particular concern. This paper therefore draws from four distinct case studies to grasp the variegated and cumulative vulnerability and resilience among local populations as they relate to internal and intraregional migration. [less ▲]

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See detailSuccess SMB:Leadership Andr Multidimensional Management. An exploratoire Study.
Robert, Jocelyne ULg

E-print/Working paper (2016)

Montre l'importance du leadership comme facteur de succès des PME en croissance rapide (gazelles ). Étude exploratoire.

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See detailContrasting Arctic and Antarctic sea ice temperatures
Vancoppenolle, M.; Raphael, M.; Rousset, C. et al

Conference (2016, April)

Sea ice temperature affects the sea ice growth rate, heat content, permeability and habitability for ice algae. Large-scale simulations with NEMO-LIM suggest large ice temperature contrasts between the ... [more ▼]

Sea ice temperature affects the sea ice growth rate, heat content, permeability and habitability for ice algae. Large-scale simulations with NEMO-LIM suggest large ice temperature contrasts between the Arctic and the Antarctic sea ice. First, Antarctic sea ice proves generally warmer than in the Arctic, in particular during winter, where differences reach up to ∼10◦C. Second, the seasonality of temperature is different among the two hemispheres: Antarctic ice temperatures are 2-3◦C higher in spring than they are in fall, whereas the opposite is true in the Arctic. These two key differences are supported by the available ice core and mass balance buoys temperature observations, and can be attributed to differences in air temperature and snow depth. As a result, the ice is found to be habitable and permeable over much larger areas and much earlier in late spring in the Antarctic as compared with the Arctic, which consequences on biogeochemical exchanges in the sea ice zone remain to be evaluated. [less ▲]

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See detailPrevalence of frailty among nursing home, according to different operational definitions.
Buckinx, Fanny ULg; Reginster, Jean-Yves ULg; GILLAIN, Sophie ULg et al

in Osteoporosis International (2016, April), 27(Supplement 1), 216-217

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See detailTen years of Denosumab (DMAB) treatment in postmenopausal women with osteoporosis. Results from the FREEDOM Extension trial.
Bone, H.G.; Brandi, M.L.; Brown, J.P. et al

in Osteoporosis International (2016, April), 27(Supplement 1), 135-136

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