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See detailDifferent and synergistic effect of 5-Azacytidine and Rapamycin on ex vivo expansion of natural human T Regulatory cells
Conteduca, Giuseppina ULg

Poster (2016, October 14)

Natural T regulatory cells (Treg) are challenging to expand ex vivo, and this has severely hindered in vivo evaluation of their therapeutic potential. 5-Azacytidine (5-azaC) and Rapamycin (RAPA) are ... [more ▼]

Natural T regulatory cells (Treg) are challenging to expand ex vivo, and this has severely hindered in vivo evaluation of their therapeutic potential. 5-Azacytidine (5-azaC) and Rapamycin (RAPA) are immunosuppressive drugs that promote selectively the expansion of CD4+CD25highFoxp3+ regulatory T cells.: We investigated whether 5-azaC and RAPA could be used together to promote the ex vivo expansion of Tregs purified from adult human peripheral blood.We found that 5-azaC helped maintain FOXP3 expression during the expansion process probably by promoting the conversion of T conventional (Tconv) in Treg, instead Rapa induces selectively apoptosis in Tconv cells and expansion in Treg. Addition of 5-azaC to RAPA treated cultures improved gene expression of FOXP3, CD25, STAT5 and TGF-B resulted in enhanced Treg expansion and suppressive activity. Also Rapa and 5-AzaC combination sustain Bcl-2 protein expression in Treg conferring resistance to apoptosis process. 5-azaC may have utility in ex vivo expansion of human Tregs, not as a single agent, but in combination with RAPA. These data may considerably accelerate the development of immunotherapeutic approaches for the treatment of autoimmune disease or posttransplant alloreactions by the adoptive transfer of nTreg cells [less ▲]

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See detailBouger à tout âge? Oui, mais …
Cloes, Marc ULg

Conference given outside the academic context (2016)

Detailed reference viewed: 12 (0 ULg)
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See detailTreg, Th17 and γδ T cells during normal and abortive pregnancy
Polese, Barbara ULg; Gridelet, Virginie ULg; Munaut, Carine ULg et al

Conference (2016, October 14)

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See detailHépatite C. Dépister largement...et guérir!
DELWAIDE, Jean ULg

in Le Journal du Médecin (2016), (2467), 20-21

Detailed reference viewed: 27 (3 ULg)
See detailThe Terra Mosana Project
Billen, Roland ULg

Conference (2016, October 14)

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See detailIntegrated continuous flow photoreactors: Photooxidation of (L)-methionine with singlet oxygen
Emmanuel, Noémie ULg; Mendoza Gallego, Carlos ULg; Lismont, Marjorie ULg et al

Poster (2016, October 13)

Development of photochemistry in macroscopic batch reaction vessels is hampered due to inherent limitations: light penetration remains superficial, resulting in inhomogeneous irradiation and hence to side ... [more ▼]

Development of photochemistry in macroscopic batch reaction vessels is hampered due to inherent limitations: light penetration remains superficial, resulting in inhomogeneous irradiation and hence to side-reactions or product degradation due to overexposure. The recent implementation of photochemical processes in microreactors under continuous-flow conditions appeared to be much more powerful than its batch analogue in terms of irradiation efficiency and light penetration. Furthermore, the fine control of residence time ensures an accurate control of the irradiation time, avoiding side-reactions and degradation. The photooxidation of (L)-methionine with singlet oxygen using Rose Bengal as a sensitizer was successfully implemented in a microreactor setup and led to total and selective conversion into methionine sulfoxide, an important building block for the organic synthesis of peptides or functionalized amino acid. The reaction was performed in 20 min while the same reaction in batch took 2h. [less ▲]

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See detailAccuracy of dichotomous logistic IRT models to estimate ability with multiple choice items
Magis, David ULg

Conference (2016, October 13)

This presentation focuse son multiple-choice items without any control or correction for guessing. Most often these items are recoded as binary outcomes (TRUE-FALSE) and logistic IRT models are calibrated ... [more ▼]

This presentation focuse son multiple-choice items without any control or correction for guessing. Most often these items are recoded as binary outcomes (TRUE-FALSE) and logistic IRT models are calibrated using these recoded data. The main purpose of this study is to highlight the limits of this approach by (a) proposing a simulation model to allow for guessing at various levels of ability, and (b) comparing the dichotomous approach to the use of nominal response model. Preliminary simulations indicate that none of the models are very accurate to estimate latent abilities accurately, though the nominal response model has fewer bias then the dichotomous models. [less ▲]

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See detailLeaving Gauss: extending Gaussian variance bounds to arbitrary targets
Clette, Emilie ULg; Swan, Yvik ULg

Conference (2016, October 13)

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See detailA Safe and Reliable Continuous-Flow System towards Methylphenidate Hydrochloride
Gerardy, Romaric ULg; Monbaliu, Jean-Christophe ULg

Poster (2016, October 13)

The batch-to-flow translation of a carbene-based strategy for the diastereoselective preparation of the active pharmaceutical ingredient threo-methylphenidate hydrochloride is presented. The continuous ... [more ▼]

The batch-to-flow translation of a carbene-based strategy for the diastereoselective preparation of the active pharmaceutical ingredient threo-methylphenidate hydrochloride is presented. The continuous-flow system implies the generation of an explosive organic azide, its consumption in a diazo transfer reaction, an intermediate purification step followed by a Rh(II)-catalyzed carbene C-H insertion reaction that produces threo-enriched N-Boc-methylphenidate. Boc-deprotection is carried out off-line and provides methylphenidate hydrochloride in 19% isolated yield (3 steps fully telescoped) or 38% isolated yield (sequential). [less ▲]

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See detailCurating Canons of Comics: Daniel Clowes and Art Spiegelman's 'Private' Museums
Crucifix, Benoît ULg

Conference (2016, October 13)

As guest of honor at the 2012 International Comics Festival in Angoulême, Art Spiegelman did not only have a retrospective exhibition devoted to his work, but was also invited to act as the curator of the ... [more ▼]

As guest of honor at the 2012 International Comics Festival in Angoulême, Art Spiegelman did not only have a retrospective exhibition devoted to his work, but was also invited to act as the curator of the CIBDI museum’s archive, producing his own ‘private’ museum, as suggested by the exhibition title Le Musée privée d’Art Spiegelman. Similarly, for Daniel Clowes’s retrospective exhibit in Columbus, the Wexner Center of the Arts hosted Eye of the Cartoonist: Daniel Clowes’s Selections from Comics History, based on Clowes’s curatorial choices from the Billy Ireland Cartoon Library and Museum. In each case, the cartoonist literally frames the history of comics and exhibits his personal canon of comics. As Spiegelman put it: “I have been allowed to highjack the Centre of Bande dessinée Museum to replace what is primarily the Francophone patrimony of comics with my own perverse and private map of what comics are.” This quote clearly embodies the dual facet of such exhibitions – collective and singular, shared and idiosyncratic – foregrounding the anchorage of individual graphic novelists within traditions of comics. Articulated around personal canons, these two exhibitions offer fresh perspectives to think about canonization in the comics world. Albeit focused on two 'canonized' graphic novelists, this case study nuances the auteurism of comics scholarship: not only are these exhibitions the result of collaborative work, they also recast the author in the role of curator. The key aspect of these exhibitions lies in a canonizing act of selection that circulates in varied material forms showcasing the authors’ influences. Mapping out how Clowes and Spiegelman frame their entanglement with the past of comics within the contexts of institutions that store its archive, I will examine the specificities of these exhibitions in relation to a body and style of historiography I call ‘authors’ histories of the comics.’ [less ▲]

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See detailImplication of AurA kinase in GBM cells chemotaxis in response to the production of CXCL12 in the subventricular zones
Willems, Estelle ULg; Dedobbeleer, Matthias ULg; LOMBARD, Arnaud ULg et al

Poster (2016, October 13)

Despite great improvement in standard therapies (i.e. surgery, radiotherapy and chemotherapy) of glioblastoma (GBM), the median survival rate is 15 months due to patient relapses. A major advance in the ... [more ▼]

Despite great improvement in standard therapies (i.e. surgery, radiotherapy and chemotherapy) of glioblastoma (GBM), the median survival rate is 15 months due to patient relapses. A major advance in the understanding of GBM recurrences has been the identification of GBM-initiating cells (GIC). GIC are thought to be deeply involved in GBM recurrences. Our lab designed a mouse model by grafting human GBM cells in the striatum. After the graft, we observed that tumors develop in the mouse striatum and that GIC specifically invade the subventricular zones (SVZ). SVZ are stem cells niches crucial for adult neurogenesis which seems particularly propitious for gliomagenesis since they are abundant in growth factors and permissive to proliferation. We therefore looked for soluble factors secreted by the SVZ environment and demonstrated that the local production of the CXCL12 chemokine in the SVZ is responsible for the GIC-directed migration. In this work, we aim to study the role GBM therapeutic resistance associated with the invasion of the SVZ. In this work, we identified a new actor of the CXCL12 pathway by the phosphoproteome analysis of U87MG cells stimulated with CXCL12: the mitotic kinase Aurora A (AurA) whose activity seems crucial for the CXCL12-dependent chemotaxis of GBM cells [less ▲]

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See detailDesign and synthesis of Fe2O3 nanoparticles/N-doped porous carbon structures as high performance electrode for lithium ion battery
Alkarmo, Walid ULg; Ouhib, Farid ULg; Aqil, Abdelhafid ULg et al

Poster (2016, October 13)

Thanks to their fascinating physical properties such as high surface area, multidimensional electron transport pathways and good mechanical strength, three dimensionally (3D) interconnected carbon porous ... [more ▼]

Thanks to their fascinating physical properties such as high surface area, multidimensional electron transport pathways and good mechanical strength, three dimensionally (3D) interconnected carbon porous frameworks have emerged as attractive materials for various electrochemical energy storage/conversion devices, including Li-ion batteries (LIBs), Li−S batteries, supercapacitors and fuel cells. In this context, a hierarchically structured macro- and mesoporous N-doped carbon with dispersed Fe2O3 nanoparticles (NDC@Fe2O3) is prepared by thermal treatment of a novel composite composed by PMMA particles decorated by graphene oxide (GO), PPy and iron salts. The NDC@Fe2O3 composite exhibited high surface area with a hierarchical pores structure. The combination of Fe2O3 nanoparticles with porous carbon to form hybrid anode has been an efficient way to maintain the electronic integrity of the whole electrode since the carbon acts as a buffer layer to accommodate the volume variation and to provide multidimensional electron transport pathways during the charge/discharge process. [less ▲]

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See detailLa palabra al pueblo: implicaciones jurídicas y políticas de la votación
Colussi, Ilaria Anna ULg

Scientific conference (2016, October 13)

The conference was focusing on the Italian referendum for modifying the Constitution, to be held in December 2016. The speech posed the attention on the Italian constitutional system and its history, on ... [more ▼]

The conference was focusing on the Italian referendum for modifying the Constitution, to be held in December 2016. The speech posed the attention on the Italian constitutional system and its history, on the substance of the proposal of reform, and legal and political implications of the vote, i.e. the possible effects on European Union stability and on Italian scenario. [less ▲]

Detailed reference viewed: 11 (0 ULg)
See detailPILs as a new active material for radical organic batteries
Aqil, Mohamed ULg; Ouhib, Farid ULg; Aqil, Abdelhafid ULg et al

Poster (2016, October 13)

Detailed reference viewed: 19 (2 ULg)
See detailSynthesis and characterization of non-isocyanate polyurethane (NIPU) hydrogels
Gennen, Sandro ULg; Grignard, Bruno ULg; Thomassin, Jean-Michel ULg et al

Poster (2016, October 13)

Due to its good biocompatibility, biodegradation and excellent mechanical properties, polyurethane (PU) is a material of choice for biomedical applications (gloves, tubing, artificial membranes…) and, as ... [more ▼]

Due to its good biocompatibility, biodegradation and excellent mechanical properties, polyurethane (PU) is a material of choice for biomedical applications (gloves, tubing, artificial membranes…) and, as hydrogels, it was used as wound dressing, soft contact lenses, drug delivery systems and scaffolds for tissue engineering. Classically, PU are synthesized by a step-growth polymerization between poly (di-)ols and poly (di-) isocyanates. Due to the toxicity of isocyanates, REACH regulations have changed and, today, there is a need to develop greener and safer route to produce isocyanate-free PUs. This contribution focus on the synthesis of NIPU hydrogels by copolymerizing a bifunctional CO2-sourced hydrophilic PEG bi-cyclic carbonate with diamines in presence of a cross- linker. The PEG bi-cyclic carbonates was prepared via a CO2/epoxide coupling reactions using a new efficient organocatalytic system based on the use of an ammonium salt (TBAI) in combination with a fluorinated alcohol. [less ▲]

Detailed reference viewed: 34 (3 ULg)
See detailAlkylcobalt as source of radicals for the controlled polymerization of less activated vinyl monomer
Demarteau, Jérémy ULg; Cordella, Daniela ULg; Kermagoret, Anthony et al

Poster (2016, October 13)

Detailed reference viewed: 10 (2 ULg)