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See detailSbCOMT (Bmr12) is involved in the biosynthesis of tricin-lignin in sorghum
Eudes, Aymerick; Dutta, Tanmoy; Deng, Kei et al

in PLoS ONE (2017), 12(6), 1-11

Lignin in plant biomass represents a target for engineering strategies towards the development of a sustainable bioeconomy. In addition to the conventional lignin monomers, namely p-coumaryl, coniferyl ... [more ▼]

Lignin in plant biomass represents a target for engineering strategies towards the development of a sustainable bioeconomy. In addition to the conventional lignin monomers, namely p-coumaryl, coniferyl and sinapyl alcohols, tricin has been shown to be part of the native lignin polymer in certain monocot species. Because tricin is considered to initiate the polymerization of lignin chains, elucidating its biosynthesis and mechanism of export to the cell wall constitute novel challenges for the engineering of bioenergy crops. Late steps of tricin biosynthesis require two methylation reactions involving the pathway intermediate selgin. It has recently been demonstrated in rice and maize that caffeate O-methyltransferase (COMT) involved in the synthesis syringyl (S) lignin units derived from sinapyl alcohol also participates in the synthesis of tricin in planta. In this work, we validate in sorghum (Sorghum bicolor L.) that the O-methyltransferase responsible for the production of S lignin units (SbCOMT / Bmr12) is also involved in the synthesis of lignin-linked tricin. In particular, we show that biomass from the sorghum bmr12 mutant contains lower level of tricin incorporated into lignin, and that SbCOMT can methylate the tricin precursors luteolin and selgin. Our genetic and biochemical data point toward a general mechanism whereby COMT is involved in the synthesis of both tricin and S lignin units. [less ▲]

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See detailUps and downs for SoTL development in a collective project targeting feedback practice enhancement
Verpoorten, Dominique ULg; Detroz, Pascal ULg; Mohr, Audrey ULg et al

Conference (2017, June 08)

The “Feedback First-Year Project” (FFYP) was designed by IFRES (Institute for Training and Research in Higher Education) and the Study Guidance Service at the University of Liège (Belgium) to support ... [more ▼]

The “Feedback First-Year Project” (FFYP) was designed by IFRES (Institute for Training and Research in Higher Education) and the Study Guidance Service at the University of Liège (Belgium) to support First-Year professors’ efforts to think and act upon their current teaching and assessment practice, and possible improvements. Amongst lessons learnt, the presentation pinpoints three major risks that a collective project like FFYP might encounter. Recommendations likely to mitigate these risks are proposed, in case others would like to replicate the experience. [less ▲]

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See detailDiscovery and Characterization of R/S-N-3-Cyanophenyl-N'-(6-tert-butoxycarbonylamino-3,4-dihydro-2,2-dimethyl-2H-1-benzopyran-4-yl)urea, a New Histone Deacetylase Class III Inhibitor Exerting Antiproliferative Activity against Cancer Cell Lines
Schnekenburger, M; Goffin, Eric ULg; Lee, J-Y et al

in Journal of Medicinal Chemistry (2017), 60(11), 4714-4733

A new series of N-aryl-N'-3,4-dihydro-2,2-dimethyl-2H-1-benzopyran-4-yl)ureas bearing an alkoxycarbonylamino group at the 6-position were synthesized and examined as putative anticancer agents targeting ... [more ▼]

A new series of N-aryl-N'-3,4-dihydro-2,2-dimethyl-2H-1-benzopyran-4-yl)ureas bearing an alkoxycarbonylamino group at the 6-position were synthesized and examined as putative anticancer agents targeting sirtuins in glioma cells. On the basis of computational docking combined to in vitro sirtuin 1/2 inhibition assays, we selected compound 18 [R/S-N-3-cyanophenyl-N'-(6-tert-butoxycarbonylamino-3,4-dihydro-2,2-dimethyl-2H-1-benzopyran-4-yl)urea] which displays a potent antiproliferative activity on various glioma cell types, assessed by quantitative videomicroscopy, eventually triggering senescence. The impact on normal glial cells was lower with a selectivity index of >10. Furthermore, human U373 and Hs683 glioblastoma cell lines served to demonstrate the inhibitory activity of 18 against histone deacetylase (HDAC) class III sirtuins 1 and 2 (SIRT1/2) by quantifying acetylation levels of histone and non-histone proteins. The translational potential of 18 was validated by an NCI-60 cell line screen and validation of growth inhibition of drug resistant cancer cell models. Eventually, the anticancer potential of 18 was validated in 3D glioblastoma spheroids and in vivo by zebrafish xenografts. In summary, compound 18 is the first representative of a new class of SIRT inhibitors opening new perspectives in the medicinal chemistry of HDAC inhibitors. [less ▲]

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See detailDéplacer pragmatiquement le débat sur la critique? Quelques gestes critiques pour repeupler le temps
Eliçabe, Rémi; Guilbert, Amandine; Pieron, Julien ULg

Scientific conference (2017, June 08)

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See detailL'art de la médiation scolaire. Entre ambiguïté et interprétations
Dethier, Baptiste ULg

Doctoral thesis (2017)

La thèse porte sur la construction de la politique de médiation scolaire menée en Belgique francophone et sa mise en œuvre concrète par de nouveaux agents chargés de l’interpréter : les médiateurs ... [more ▼]

La thèse porte sur la construction de la politique de médiation scolaire menée en Belgique francophone et sa mise en œuvre concrète par de nouveaux agents chargés de l’interpréter : les médiateurs scolaires. Dans un milieu scolaire où les acteurs, internes et externes aux établissements, sont multiples, les médiateurs sont appelés à prendre en charge des problèmes scolaires tels que la violence, le décrochage et l’absentéisme dans un cadre ambigu et, par conséquent, sujet à interprétation. La thèse rend compte du contexte organisationnel dans lequel les médiateurs s’inscrivent et de la mise en œuvre de cette politique. Baptiste Dethier analyse leur travail à l’aide de la métaphore du travail « artistique », c’est-à-dire comme un ensemble de procédures, de connaissances et de règles encadrant l’exercice d’une activité créative. Tout d’abord, cette activité est construite à partir d’orientations de sens – et non de prescriptions – données par l’État, les médiateurs scolaires étant chargés de les interpréter, grâce à une autonomie partiellement contrôlée. Ensuite, ces interprétations sont analysées grâce aux discours des médiateurs, reflétant la forte hétérogénéité dans la mise en œuvre de la politique de médiation scolaire ainsi que les controverses qui animent en permanence la fonction de médiateur. Enfin, à travers l’analyse des pratiques concrètes des médiateurs, il apparaît qu’elles consistent : d’une part, en la création de mises en scène spécifiques destinées à produire chez les bénéficiaires un état particulier de sensibilité ; d’autre part, comme autant de mises à l’épreuve de leur intégrité « artistique » et des principes dont ils se revendiquent. La thèse montre que les médiateurs scolaires se distinguent moins par ce qu’ils font que par la façon dont ils en parlent. Dès lors, la place de la place de la médiation et de la fonction de médiateur dans la division du travail au sein des organisations scolaires est questionnée. Celle-ci s’y trouve éclatée et ambigüe, entre contre-culture bousculant les équilibres historiques du système et participation au maintien de la forme scolaire traditionnelle. [less ▲]

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See detailRetours d’expérience d’un partenariat Université - Agences de l'Eau pour une gestion de la qualité des eaux de surface
Deliège, Jean-François ULg; Everbecq, Etienne ULg; MAGERMANS, POL ULg et al

Conference (2017, June 08)

Un partenariat innovant a été établi entre une équipe de recherche universitaire (unité R&D de l’Aquapôle, Université de Liège) et des pouvoirs publics ayant dans leurs attributions la protection des ... [more ▼]

Un partenariat innovant a été établi entre une équipe de recherche universitaire (unité R&D de l’Aquapôle, Université de Liège) et des pouvoirs publics ayant dans leurs attributions la protection des ressources en eau et des milieux aquatiques (Agences de l’Eau françaises, Administrations wallonne et flamande). L’objectif principal de ce partenariat est la gestion des ressources par les Agences et Administrations en les dotant d’un outil innovant et opérationnel d’aide à la décision leur permettant de remplir leurs missions régionales (programmes d’intervention, SDAGE, …), nationales et internationales (DCE, rapportage européen, …). Pour atteindre cet objectif, le partenariat se base sur le développement d’une suite logicielle répondant aux attentes des opérateurs. Cette stratégie de développement, basée sur les besoins des partenaires, est au bénéfice direct de la restauration de la qualité des milieux aquatiques. [less ▲]

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See detailCharacterization of the pre-metastatic niche in lymph node, in experimental and clinical settings
Noël, Agnès ULg; Balsat, Cédric; Garcia Caballero, Melissa et al

Poster (2017, June 08)

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See detailFire resistance of concrete slabs acting in compressive membrane action
Molkens, Tom; Gernay, Thomas ULg; Caspeele, Robby

in Nigro, Emidio; Bilotta, Antonio (Eds.) Proceedings of the 2nd International Fire Safety Symposium - IFireSS 2017 (2017, June 08)

In building renovation, the real behaviour of reinforced concrete slabs cannot always be explained by the bending theory according to classical structural mechanics. Indeed, the bearing capacity, as ... [more ▼]

In building renovation, the real behaviour of reinforced concrete slabs cannot always be explained by the bending theory according to classical structural mechanics. Indeed, the bearing capacity, as assessed for instance by a loading test, sometimes appears to be much higher than what would be expected. This phenomenon may be caused by the activation of an arch-effect or so-called compressive membrane action (CMA) which can develop even with small vertical deformations. For a slab which is completely restrained, the presence of reinforcement becomes of lesser importance when this phenomenon is activated (except for end fields). Hence, for fire resistance purposes, it can be discussed whether reinforcement and concrete cover has a smaller influence on the bearing capacity for slabs subjected to fire which exhibit a significant concrete compressive membrane behaviour. This paper presents a loading test performed on a real concrete building which highlighted the development of CMA as the load bearing mode. It then proposes a strategy to evaluate the behaviour resulting from the development of CMA in reinforced concrete slabs at ambient and at elevated temperature based on numerical modelling. The numerical analyses are performed with the finite element software SAFIR® using a strip of layered shell elements. A plastic-damage constitutive model with an explicit transient creep formulation is used to capture the concrete behaviour at elevated temperature. [less ▲]

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See detailDu plus petit au plus grand
Beckers, Albert ULg

Scientific conference (2017, June 08)

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See detailMolecular surveillance of Plasmodium falciparum resistance to artemisinin-based combination therapies in the Democratic Republic of Congo
Mvumbi Makaba, Dieudonné; Bobanga Lengu, Thierry; Kayembe Ntumba, Jean-Marie et al

in PLoS ONE (2017)

Malaria is a major public health problem in the Democratic Republic of Congo. Despite progress achieved over the past decade in the fight against malaria, further efforts have to be done such as in the ... [more ▼]

Malaria is a major public health problem in the Democratic Republic of Congo. Despite progress achieved over the past decade in the fight against malaria, further efforts have to be done such as in the surveillance and the containment of Plasmodium falciparum resistant strains. We investigated resistance to artemisinin-based combination therapies currently in use in Democratic Republic of Congo by surveying molecular polymorphisms in three genes: pfcrt, pfmdr1 and pfk13 to explore possible emergence of amodiaquine, lumefantrine or artemisinin resistance in Democratic Republic of Congo. This study essentially revealed that resistance to chloroquine is still decreasing while polymorphism related to amodiaquine resistance seems to be not present in Democratic Republic of Congo, that three samples, located in the east of the country, harbor Pfmdr1 amplification and that none of the mutations found in South-East Asia correlated with artemisinine resistance have been found in Democratic Republic of Congo. But new mutations have been identified, especially the M476K, occurred in the same position that the M476I previously identified in the F32-ART strain, strongly resistant to artemisinine. Antimalarial first-line treatments currently in use in Democratic Republic of Congo are not associated with emergence of molecular markers of resistance. [less ▲]

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See detailPromouvoir, développer et optimiser les pratiques de feedback formatif en Bloc1 en Faculté d'Architecture - Partage d'expériences
Silvestre, Aude ULg; Seijkens, Nicolas ULg

Scientific conference (2017, June 08)

Au cours des quatre dernières années, l’Institut de Formation et de Recherche en Enseignement Supérieur (IFRES) et le Service Guidance Étude (SGE) de l’Université de Liège ont développé et mis en place un ... [more ▼]

Au cours des quatre dernières années, l’Institut de Formation et de Recherche en Enseignement Supérieur (IFRES) et le Service Guidance Étude (SGE) de l’Université de Liège ont développé et mis en place un projet visant à promouvoir et optimiser les pratiques de feedback formatif et d’Assessment for Learning en première année. Le Projet Feedback a été initié en 2013 et poursuivi en 2014 au sein de la Faculté des Sciences appliquées, adapté depuis juin 2014 en Faculté de Droit, de Science Politique et de Criminologie, depuis 2015 en Faculté des Sciences, et depuis 2016 en Faculté d’Architecture. Le projet est mené dans un but de soutien à l’apprentissage et à la qualité de l’expérience vécue par les étudiants de première année. Il vise à engager un groupe d’enseignants en Architecture dans une réflexion portant sur leurs pratiques d’enseignement et d’évaluation, et de potentielles régulations à y apporter. Chacun des participants impliqués est porté à réenvisager certaines dimensions de son enseignement de première année à la lumière des théories se rapportant aux pratiques de feedback lors d’entretiens individuels structurés, avec l’équipe pédagogique encadrante (une référente pédagogique et une architecte), mais également lors de séances plénières collectives. Des initiatives, telles que des tests de prérequis en mathématique, ou une grille de critères pour le dessin ou encore outil réflexif d’aide à la prise de note en atelier de projet, ont été développées dans divers cours (Mathématique ; Théorie de l’Architecture ; Construction et d’Applications Constructives ; Atelier de Projet ; etc.). Parmi la quinzaine d’initiatives élaborées, certaines ont déjà été mises en œuvre, d’autres sont toujours en développement actuellement ou sont en phase de réflexion. L’avis des étudiants est au cœur des préoccupations du Projet Feedback. Plusieurs questionnaires ont été administrés aux étudiants, afin d’investiguer leurs perceptions relatives à diverses initiatives mises en place lors de l’année académique 2016-2017. [less ▲]

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See detailExemples tirés de la pratique: les phanères
Hayette, Marie-Pierre ULg

Conference (2017, June 08)

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See detailDiametral dimension and property Omega Bar for spaces Snu
Demeulenaere, Loïc ULg

Conference (2017, June 08)

Spaces Snu are metrizable sequence spaces defined by Jaffard in the context of multifractal analysis and signal treatment. From a functional analysis point of view, the study of these spaces points out ... [more ▼]

Spaces Snu are metrizable sequence spaces defined by Jaffard in the context of multifractal analysis and signal treatment. From a functional analysis point of view, the study of these spaces points out some topological properties, such as the facts they are locally pseudoconvex in general and locally p-convex in certain cases, Schwartz, and non-nuclear. In this talk, we focus on two topological invariants, namely the diametral dimension (Bessaga, Mityagin, Pelczynski, Rolewicz) and the property "Omega Bar" (Vogt, Wagner). Firstly, we revisit a result of Aubry and Bastin giving the diametral dimension of locally p-convex spaces Snu and extend it to some non-locally p-convex spaces Snu. Secondly, we explain how these developments can be used to prove that a subclass of spaces Snu (among which the locally p-convex ones) verifes the condition Omega Bar. [less ▲]

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See detailOccurrence and fate of emerging organic contaminants (EOCs) in the aquifers of Barcelona
Jurado Elices, Anna ULg

Scientific conference (2017, June 07)

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See detailFire risk assessment of multi-story buildings based on fragility analysis
Gernay, Thomas ULg; Elhami Khorasani, Negar; Garlock, Maria

in Nigro, Emidio; Bilotta, Antonio (Eds.) Proceedings of the 2nd International Fire Safety Symposium - IFireSS 2017 (2017, June 07)

Recent efforts aim at assessing the fire performance of structures in a probabilistic framework. But there is still no well-established method to quantify the reliability of entire buildings. Previous ... [more ▼]

Recent efforts aim at assessing the fire performance of structures in a probabilistic framework. But there is still no well-established method to quantify the reliability of entire buildings. Previous works focused on isolated structural members, therefore not allowing for a determination of the global safety level of buildings. Here, a new methodology is developed to quantify the reliability of buildings in fire. The methodology uses Monte Carlo simulations for constructing fragility functions associated with different fire breakout locations in a building, then combines the functions to characterize the overall building conditional probability of failure, and finally incorporates the probabilistic models for intensity measure and fire occurrence likelihood. The methodology is applied to multi-story steel buildings. This work addresses fire reliability at the building scale, and therefore is useful for standardizing safety level as well as for evaluating community resilience. [less ▲]

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See detailProbing Single Molecules with the AFM: Force, Motion, Dynamics, and Function
Duwez, Anne-Sophie ULg

Conference (2017, June 07)

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