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See detailDynamic single-cell analysis of Saccharomyces cerevisiae under process perturbation: comparison of different methods for monitoring the intensity of population heterogeneity
Delvigne, Frank ULg; Baert, Jonathan ULg; Gofflot, Sébastien et al

in Journal of Chemical Technology & Biotechnology (in press)

BACKGROUND: Single cell biology has attracted a lot of attention these past few years and has led to numerous fundamental results pointing out the heterogeneity of clonal cell populations. In this context ... [more ▼]

BACKGROUND: Single cell biology has attracted a lot of attention these past few years and has led to numerous fundamental results pointing out the heterogeneity of clonal cell populations. In this context, microbial phenotypic heterogeneity under bioprocessing conditions needs to be further investigated. In this study, yeast based processes have been investigated by using on-line flow cytometry in combination with a fluorescent transcriptional reporter (GFP) and viability fluorescence tags (propidium iodide, PI). Methods aiming at expressing the dispersion of these fluorescence tags among the yeast populations have been investigated for different bioreactor operating conditions. RESULTS: Yeast viability was determined on the basis of PI uptake. Segregation between PI negative and positive subpopulations could be efficiently quantified on the basis of the mean-to-median ratio or the amplitude of the interquartile range. On the other hand, the same quantification could not be made for the segregation occurring at the level of GFP synthesis. Indeed, when cells were exposed to sub-lethal or mild stresses (such as in scale-down reactors) two GFP subpopulations could be visualized by real-time FC, but quantification by one of the above-mentioned methods was not possible. CONCLUSIONS: Yeast population heterogeneity was observed in representative bioreactor operating conditions. Difficulties for the determination of segregation at the level of GFP synthesis point out the fact that one needs to understand the segregation mechanisms for the applied fluorescent reporters, to judge whether simple mathematical tools may be applied or if more sophisticated computational tools are needed for the quantification of the microbial population segregation. [less ▲]

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See detailThe transition to conservation agriculture : an insularization process towards sustainability
Vankeerberghen, Audrey ULg; Stassart, Pierre M ULg; Dannevoye, Bastien ULg

in International Journal of Agricultural Sustainability (in press)

Detailed reference viewed: 79 (21 ULg)
See detailDynamics of Environmental Migration
Gemenne, François ULg; Jäger, Jill; Entzinger, Han

Book published by Palgrave Macmillan (in press)

Detailed reference viewed: 52 (5 ULg)
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See detailConfusion de codes graphiques dans les papyrus latins
Rochette, Bruno ULg; Nocchi Macedo, Gabriel

in Cappasso, Mario (Ed.) Mélanges à la mémoire de Paolo Radiciotti (in press)

Detailed reference viewed: 23 (6 ULg)
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See detailGünter Grass and his translators: from a collaborative dynamic to a controlling apparatus?
Letawe, Céline ULg

in Cordingley, Anthony; Frigau Manning, Céline (Eds.) Collaborative Translation: from the Renaissance to the Digital Age (in press)

Entre 1978 et 2013, Günter Grass a réuni ses traducteurs autour de chacun de ses romans pour qu’ils puissent discuter avec lui et entre eux des problèmes de traduction. L’expérience fascine, mais elle ... [more ▼]

Entre 1978 et 2013, Günter Grass a réuni ses traducteurs autour de chacun de ses romans pour qu’ils puissent discuter avec lui et entre eux des problèmes de traduction. L’expérience fascine, mais elle pose aussi de nombreuses questions. A partir de correspondances, de protocoles et d’enregistrements conservés aux archives et de comparaisons entre l’original de Günter Grass et plusieurs traductions, il a été possible non seulement de retracer le processus, mais aussi d’en évaluer le résultat, pour ainsi mieux cerner les enjeux multiples liés à ces séminaires de traducteurs. A côté de leur finalité avouée (l’amélioration de la qualité des traductions), ils participent à un système de contrôle plus large et contribuent à légitimer les traductions et à en faire la publicité, mais ils ont aussi pour effet de créer une dynamique entre les traducteurs et de leur donner davantage de visibilité. [less ▲]

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See detailState Created Barriers to Exit - The Example of the Acquisition of Alstom by General Electric
Petit, Nicolas ULg

in Competition Policy International (in press)

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See detailSur les pas des hommes qui ont vu l’ours. La conservation de la faune sauvage, un travail réel.
Denayer, Dorothée ULg; Mougenot, Catherine ULg; Collard, Damien

in Natures Sciences Sociétés (in press)

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See detailLe socio-écosystème : dynamiques institutionnelles et interdisciplinarité.
Denayer, Dorothée ULg; Couvet, Denis

in Interdisciplinarités entre Natures et Sociétés. (in press)

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See detailLes animaux comme révélateurs et passeurs de frontières.
Roué, Marie; Manceron, Vanessa; Denayer, Dorothée ULg et al

in Interdisciplinarités entre Natures et Sociétés. (in press)

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See detailStructural analysis of monometallic ruthenium–arene complexes using ion mobility mass spectrometry, collision-induced dissociation, and density functional theory
Cserwinska, Izabella; Far, Johann ULg; Kune, Christopher ULg et al

in Journal of the Chemical Society. Dalton Transactions (in press)

Ion mobility mass spectrometry (IM-MS) and collision-induced dissociation (CID) techniques were used to investigate the influence of the phosphine ligand on the physicochemical properties of [RuCl2(p ... [more ▼]

Ion mobility mass spectrometry (IM-MS) and collision-induced dissociation (CID) techniques were used to investigate the influence of the phosphine ligand on the physicochemical properties of [RuCl2(p-cymene)(PCy3)] (1), [RuCl2(p-cymene)-(PPh3)] (2), and [RuCl2(p-cymene)(PTA)] (3) in the gas phase (PTA is 1,3,5-triaza-7-phosphaadamantane). Electrospray ionization of complexes 1 and 2 led to the corresponding [RuCl(p-cymene)(PR3)]+ ions via the dissociation of a chlorido ligand, whereas RAPTA-C (3) afforded two molecular ions by in-source oxidation ([RuIIICl2(p-cymene)(PTA)]+) or protonation ([RuCl2(p-cymene)(PTA+H)]+). Control experiments showed that the balance between these two ionization paths was strongly influenced by the nature of the solvent used for infusion. Collision cross sections (CCSs) of the four molecular ions accurately reflected the variations of steric bulk inferred from the Tolman steric parameters (θ) of the phosphine ligands. Moreover, DFT calculations combined with a model based on the kinetic theory of gases (the trajectory method of the IMoS software) afforded reliable CCS predictions. The almost two times higher dipole moment of [RuCl2(p-cymene)-(PTA+H)]+ (μ = 13.75 D) compared to [RuIIICl2(p-cymene)(PTA)]+ (μ = 7.18 D) was held responsible for increased ion-induced dipole interactions with a polarizable drift gas such as N2. Further experiments with He and CO2 confirmed that increasing the polarizability of the buffer gas improved the separation between the two molecular ions derived from complex 3. The fragmentation patterns of complexes 1–3 were determined by CID. The sequence of collision voltages at which 50% of a precursor ion dissociates (V50) recorded for the molecular ions derived from compounds 1–3 was in good agreement with simple electronic considerations based on the donor strength of the phosphine ligand. Thus, the CCS and V50 parameters used to determine the shape and stability of ionic species in the gas phase are complementary to the Tolman steric and electronic parameters (θ and TEP) commonly used by organometallic chemists in condensed phases. [less ▲]

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See detailBehavioural Diagnosis of Disorders of Consciousness
Thibaut, Aurore ULg; Di Perri, Carol ULg; Bodart, Olivier ULg et al

in Rossetti, Andrea; Laureys, Steven (Eds.) Clinical Neurophysiology in Disorders of Consciousness (in press)

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See detailBattista Guarino, 'Sermone del cane e del cavallo'. Un texte inconnu à la cour des Este
Valenti, Gianluca ULg; Van den Abeele, Baudouin

in Actes du Colloque international Chevaux, chiens, faucons. L’art vétérinaire antique et médiéval à travers les sources écrites, archéologiques et iconographiques (in press)

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See detailPromoting Competition at the Digital Age with an Application to Belgium
Copée, Pierre ULg; Gautier, Axel ULg; Lefevre, Mélanie ULg

in Crew, Michael; Brennan, Timothy (Eds.) The Future of the Postal Sector in a Digital World (in press)

In this paper, we develop a fully-fledged postal market model to assess the extent of competition in the mail market in a context of declining mail volumes due to e-substitution. Our focus will be on the ... [more ▼]

In this paper, we develop a fully-fledged postal market model to assess the extent of competition in the mail market in a context of declining mail volumes due to e-substitution. Our focus will be on the Belgian market situation that is interesting for at least two reasons. First, the homogenous nature of the country implies that the extent of the entrant's market coverage will be either limited to the few very densely populated areas or large covering the whole country at the exception of the few very sparely populated areas. Second, because strong licensing requirements -including coverage constraints- are imposed on competing postal operators and they may constitute barrier to entry. Our model allows us to estimate the extent of entry, the cost of meeting the licensing requirements for potential entrants and the impact of entry on the cost of the universal service. [less ▲]

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See detailTransnational Ageing and the ‘Zero Generation’: The Role of Moroccan Migrants’ Parents in Care Circulation
Zickgraf, Caroline ULg

in Journal of Ethnic and Migration Studies (in press)

This article emphasizes the impacts of international migration on ageing processes and norms within transnational families, and, specifically, analyses the roles of the zero generation, defined as the ... [more ▼]

This article emphasizes the impacts of international migration on ageing processes and norms within transnational families, and, specifically, analyses the roles of the zero generation, defined as the parents of first-generation migrants (Nedelcu 2009), both as non-migrant counterparts to their children abroad and as ageing migrants themselves. Transnational caregiving research has demonstrated that for many migrants and their families care arrangements must be negotiated across national borders, yet the agency of transnational older adults has been largely neglected. Based on qualitative fieldwork conducted from 2011 to 2013 in Liège, Belgium and Oujda, Morocco, the present article examines the circulation of care between Moroccan adult migrants and their ageing ascendants. This article exposes the duality of migrants’ ascendants as care givers and recipients, asserting firstly, that receiving care is not synonymous with passivity, and that, secondly, migrants’ ascendants are also the initiators of transnational practices. In both roles, this article underlines mobility as a strategy used by migrants’ parents to maintain family solidarity across national borders, but that this care mobility is limited by macro-level migration policies, here exemplified by the Belgian migration regime. [less ▲]

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See detail« Left Behind »? Le rôle des non-migrants dans les dynamiques de soins transnationales
Zickgraf, Caroline ULg

in Bousetta, Hassan; Jacquemain, Marc; Martiniello, Marco (Eds.) et al Transnationalisme, dynamique des identités et diversification culturelle dans les villes post-migratoires (in press)

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See detailA fresh look on the limit on ultralight axion-like particles from SN1987A
Payez, Alexandre ULg

in 10th Patras Workshop on Axions, WIMPs and WISPs (in press)

We revisit the limit on very light axion-like particles (ALPs) from the absence of gamma rays coincidental with the neutrino burst from SN1987A. We use updated supernova simulations, modern models for the ... [more ▼]

We revisit the limit on very light axion-like particles (ALPs) from the absence of gamma rays coincidental with the neutrino burst from SN1987A. We use updated supernova simulations, modern models for the magnetic field inside the Galaxy, and a Primakoff cross section which takes into account proton-degeneracy and mass-reduction effects. We finally give an updated exclusion plot for the electromagnetic coupling of sub-eV ALPs, comparing our new bound with other limits as well as with future ALP searches. [less ▲]

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See detailDisorders of consciousness: new advances in neuroimaging techniques
Soddu, Andrea ULg; Bruno, Marie-Aurélie ULg; VANHAUDENHUYSE, Audrey ULg et al

in Zanotti, Bruno (Ed.) Vegetative State (in press)

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See detailFin de siècle/Jahrhundertwende: Eine Tendenzgeschichte
Küpper, Achim ULg

in Roland, Hubert (Ed.) Eine kleine deutsch-französische Literaturgeschichte vom 18. Jahrhundert bis zum Ersten Weltkrieg (in press)

Detailed reference viewed: 33 (3 ULg)
See detailAlbum zutique - Dixains réalistes
Saint-Amand, Denis ULg; Grojnowski, Daniel

Book published by Garnier Flammarion (in press)

Édition de deux recueils collectifs emblématiques de la poésie française du XIXe siècle, comprenant une introduction, des notes explicatives pour chaque texte, un dossier et un dictionnaire des auteurs.

Detailed reference viewed: 18 (0 ULg)