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See detailComplementary biophysical tools to investigate lipid specificity in the interaction between bioactive peptides and the plasma membrane
Deleu, Magali ULg; Lins, Laurence ULg

Conference (2017, March)

Plasma membranes are complex entities common to all living cells. The basic principle of their organization appears very simple, but they are actually of high complexity and represent very dynamic ... [more ▼]

Plasma membranes are complex entities common to all living cells. The basic principle of their organization appears very simple, but they are actually of high complexity and represent very dynamic structures. The interactions between bioactive molecules notably peptides, and lipids are important for numerous processes, from drug bioavailability to viral fusion. The cell membrane is a carefully balanced environment and any changes inflicted upon its structure by a bioactive peptide must be considered in conjunction with the overall effect that this may have on the function and integrity of the membrane. As a general concept, understanding the mechanism at the molecular level by which bioactive molecules interact with cell membranes is of fundamental importance. Lipid specificity is a key factor for the detailed understanding of the penetration and/or activity of lipid-interacting peptides and of mechanisms of some diseases. Further investigation in that way should improve drug discovery and development of membrane-active molecules in many domains such as health, plant protection or microbiology. In this talk, we propose to overview some complementary “in vitro” and “in silico” biophysical approaches that can give information about lipid specificity at a molecular point of view. Our strategy is illustrated on different bioactive peptides such as antimicrobial peptides, peptides involved in human diseases or in plant cell signalling. [less ▲]

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See detailINFECTIVITÉ IN VIVO ET RÉCUPÉRATION IN VITRO DE L’APTITUDE (FITNESS) RÉPLICATIVE D’UN NOROVIRUS MURIN RECOMBINANT
De Oliveira-Filho, Edmilson; Ludwig, Louisa ULg; Mathijs, Elisabeth et al

Scientific conference (2017, March)

Les norovirus sont reconnus comme étant une cause majeure d’épidémies de gastroentérites chez l’homme. Les mutations ponctuelles et la recombinaison sont les mécanismes moléculaires moteurs de l’évolution ... [more ▼]

Les norovirus sont reconnus comme étant une cause majeure d’épidémies de gastroentérites chez l’homme. Les mutations ponctuelles et la recombinaison sont les mécanismes moléculaires moteurs de l’évolution des norovirus. La recombinaison peut créer des modifications considérables dans les génomes viraux. Nous avons récemment obtenu un norovirus murin recombinant (RecMNV) après coinfection in vitro de souches homologues de norovirus murins. Le RecMNV présente une aptitude (fitness) réduite à la réplication. L'infectivité du RecMNV a été d’abord évaluée in vivo et ses adaptations réplicatives et génétiques ont été ensuite suivies au cours de passages en série in vitro. Durant l'infection in vivo, le RecMNV a été comparé aux souches parentales chez des souris Balb/cByJ par la mesure du poids corporel et l'estimation de la charge virale par RT-qPCR. Le poids des souris infectées par RecMNV était généralement plus élevé que celui des souris infectées par les souches parentales. Des charges virales ont été détectées dans tous les organes examinés, suggérant que, comme les souches parentales, le RecMNV peut se propager au-delà du tube digestif pour produire des infections systémiques. Ensuite, les générations de RecMNV résultant du premier (RecE) et du dixième (RecL) passages in vitro ont été comparées et leurs séquences déterminées. RecL a montré une augmentation significative du diamètre des plaques de lyse et une cinétique de réplication plus rapide que RecE. L'analyse moléculaire a montré plusieurs modifications de la séquence nucléotidique et en acides aminés entre RecE et les souches parentales ainsi qu’entre RecE et RecL. Ces résultats suggèrent que la recombinaison se produisant in vitro entre des souches de norovirus murins homologues peut générer une souche chimère, qui présente des propriétés biologiques similaires aux souches parentales et est capable d'une infection productive in vivo. De plus, ils montrent l'adaptation virale et la récupération de la réplication virale in vitro après un événement de recombinaison. [less ▲]

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See detailRussie – Venezuela : l’alliance stratégique à l’épreuve des faits
Regnier, Cindy ULg

E-print/Working paper (2017)

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See detailFeasibility Study of PV & Li-Ion Battery Based Micro-Grids for Bolivian Off-Grid Communities
Balderrama Subieta, Sergio Luis ULg; Tarantino, Andrea; Sabatini, Stefano et al

in Proceedings of IRES 2017 - The 11th International Renewable Energy Storage Conference (2017, March)

In the rural areas of Bolivia, where about a third of the people lacks access to reliable electricity, both a complex geography and a scattered population make the costs of extending the national grid ... [more ▼]

In the rural areas of Bolivia, where about a third of the people lacks access to reliable electricity, both a complex geography and a scattered population make the costs of extending the national grid prohibitively high. As an alternative, we evaluate the feasibility of an isolated micro-grid, composed by Li-ion batteries and Photovoltaic (PV) panels, for a Bolivian remote community living without access to electricity. We surveyed two remote rural villages to assess the potential electricity consumption patterns of the population once provided access to electricity. We estimated the average demand profile using the tool LoadProGen, and we collected local techno-economic and solar irradiation data of the zone. We employed such data as inputs in a robust linear programming-based optimization tool to determine the capacities of the Li-ion battery bank and the PV array thatminimize the net present cost (NPC) of the system. [less ▲]

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See detailDe l'impasse au compromis: deux expériences belges
Verjans, Pierre ULg

in Nachi, Mohamed (Ed.) Révolution & compromis. Invention d'une solution aux incertitudes de la transition démocratique en Tunisie (2017)

Lors de l'expérience fondatrice de la Belgique en 1830, aucune institution consensuelle au sens d'Arend Lijphart n'existait et un compromis fut trouvé entre les cléricaux et les anticléricaux. De 2007 à ... [more ▼]

Lors de l'expérience fondatrice de la Belgique en 1830, aucune institution consensuelle au sens d'Arend Lijphart n'existait et un compromis fut trouvé entre les cléricaux et les anticléricaux. De 2007 à 2011, toutes les institutions consensuelles identifiées par Arend Lijphart existaient mais il fallut cinq ans pour débloquer la situation. Ces exemples montrent que des institutions de consensus ne suffisent pas, il faut encore que les acteurs politiques adoptent des référentiels de compromis et aient des intérêts concrets au compromis. [less ▲]

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See detailcrossroads, le magazine sonore du blues et des musiques américaines
Sacré, Robert ULg

Diverse speeche and writing (2017)

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See detailSwitching Costs in Two-sided Markets
Lam, Wing Man Wynne ULg

in Journal of Industrial Economics (2017), 65(1), 136-182

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See detailService de numérisation à la demande dans les Bibliothèques de l'Université de Liège : Rapport sur la phase pilote de mars à décembre 2016
Simon, Stéphanie ULg; Bougrine, Hassan ULg; Renaville, François ULg

Report (2017)

Le système de gestion de bibliothèque Alma intègre un processus de gestion des documents numériques (stockage et/ou fourniture). Une analyse préalable sur les possibilités, avantages et limites d'un ... [more ▼]

Le système de gestion de bibliothèque Alma intègre un processus de gestion des documents numériques (stockage et/ou fourniture). Une analyse préalable sur les possibilités, avantages et limites d'un service de numérisation à la demande géré par Alma, dans le contexte des Bibliothèques de l'Université, a été réalisée en 2015. L'analyse a suggéré la mise en place, pour une phase pilote, d'un service de numérisation partielle à destination des membres du personnel. Le présent rapport fait le point sur cette phase pilote (mars-décembre 2016) et adresse des recommandations pour l'avenir. [less ▲]

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See detailInvestigation of the copolymerization behavior of a cyclic carbonate with vinyl acetate using CMRP
Scholten, Philip ULg; Demarteau, Jérémy ULg; Debuigne, Antoine ULg et al

Poster (2017, March)

Ethylene is an unconjugated monomer difficult to copolymerise in a controlled fashion using common radical or coordination-insertion polymerisation. Cobalt-mediated radical polymerisation (CMRP) provides ... [more ▼]

Ethylene is an unconjugated monomer difficult to copolymerise in a controlled fashion using common radical or coordination-insertion polymerisation. Cobalt-mediated radical polymerisation (CMRP) provides a powerful means to radically copolymerise ethylene in a controlled fashion under mild conditions.[1][2] In addition, different copolymer structures, such as block or alternating, can be accessed. ACO2-based cyclic carbonate was chosen as a promising comonomer but conflicting reports on the copolymer structure have been reported.[3][4] To better understand the copolymerisation behaviour of this monomer, model copolymerisations with vinyl acetate were performed and are reported below with the aim of gaining insights into the copolymersiation behaviour, which will allow us to better tackle ethylene copolymerisations. [less ▲]

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See detailInactivation of the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 cells by a multiplex CRISPR/Cas9 strategy results in glycoproteins without plant-specific glycans
Mercx, Sébastien; Smargiasso, Nicolas ULg; Chaumont, François et al

in Frontiers in Plant Science (2017), 8

Plants or plant cells can be used to produce pharmacological glycoproteins such as antibodies or vaccines. However these proteins carry N-glycans with planttypical residues [β(1,2)-xylose and core α(1,3 ... [more ▼]

Plants or plant cells can be used to produce pharmacological glycoproteins such as antibodies or vaccines. However these proteins carry N-glycans with planttypical residues [β(1,2)-xylose and core α(1,3)-fucose], which can greatly impact the immunogenicity, allergenicity, or activity of the protein. Two enzymes are responsible for the addition of plant-specific glycans: β(1,2)-xylosyltransferase (XylT) and α(1,3)- fucosyltransferase (FucT). Our aim consisted of knocking-out two XylT genes and four FucT genes (12 alleles altogether) in Nicotiana tabacum BY-2 suspension cells using CRISPR/Cas9. Three XylT and six FucT sgRNAs were designed to target conserved regions. After transformation of N. tabacum BY-2 cells with genes coding for sgRNAs, Cas9, and a selectable marker (bar), transgenic lines were obtained and their extracellular as well as intracellular protein complements were analyzed by Western blotting using antibodies recognizing β(1,2)-xylose and α(1,3)-fucose. Three lines showed a strong reduction of β(1,2)-xylose and α(1,3)-fucose, while two lines were completely devoid of them, indicating complete gene inactivation. The absence of these carbohydrates was confirmed by mass spectrometry analysis of the extracellular proteins. PCR amplification and sequencing of the targeted region indicated small INDEL and/or deletions between the target sites. The KO lines did not show any particular morphology and grew as the wild-type. One KO line was transformed with genes encoding a human IgG2 antibody. The IgG2 expression level was as high as in a control transformant which had not been glycoengineered. The IgG glycosylation profile determined by mass spectrometry confirmed that no β(1,2)-xylose or α(1,3)-fucose were present on the glycosylation moiety and that the dominant glycoform was the GnGn structure. These data represent an important step toward humanizing the glycosylation of pharmacological proteins expressed in N. tabacum BY-2 cells. © 2017 Mercx, Smargiasso, Chaumont, De Pauw, Boutry and Navarre. [less ▲]

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See detailGeneration of High-Amylose Rice through CRISPR/Cas9-Mediated Targeted Mutagenesis of Starch Branching Enzymes
Sun, Yongwei ULg; Xia, Lanqin; Francis, Frédéric ULg et al

in Frontiers in Plant Science (2017), 8

Cereals high in amylose content (AC) and resistant starch (RS) offer potential health benefits. Previous studies using chemical mutagenesis or RNA interference have demonstrated that starch branching ... [more ▼]

Cereals high in amylose content (AC) and resistant starch (RS) offer potential health benefits. Previous studies using chemical mutagenesis or RNA interference have demonstrated that starch branching enzyme (SBE) plays a major role in determining the fine structure and physical properties of starch. However, it remains a challenge to control starch branching in commercial lines. Here, we use CRISPR/Cas9 technology to generate targeted mutagenesis in SBEI and SBEIIb in rice. The frequencies of obtained homozygous or bi-allelic mutant lines with indels in SBEI and SBEIIb in T0 generation were from 26.7 to 40%. Mutations in the homozygous T0 lines stably transmitted to the T1 generation and those in the bi-allelic lines segregated in a Mendelian fashion. Transgene-free plants carrying only the frame-shifted mutagenesis were recovered in T1 generation following segregation. Whereas no obvious differences were observed between the sbeI mutants and wild type, sbeII mutants showed higher proportion of long chains presented in debranched amylopectin, significantly increased AC and RS content to as higher as 25.0% and 9.8%, respectively, and thus altered fine structure and nutritional properties of starch. Taken together, our results demonstrated for the first time the feasibility to create high-amylose rice through CRISPR/Cas9-mediated editing of SBEIIb. [less ▲]

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See detailFeasibility Study of PV & Li-Ion Battery Based Micro-Grids for Bolivian Off-Grid Communities
Balderrama Subieta, Sergio Luis ULg; Tarantino, Andrea; Sabatini, Stefano et al

in Proceedings of IRES 2017 - The 11th International Renewable Energy Storage Conference (2017, March)

In the rural areas of Bolivia, where about a third of the people lacks access to reliable electricity, both a complex geography and a scattered population make the costs of extending the national grid ... [more ▼]

In the rural areas of Bolivia, where about a third of the people lacks access to reliable electricity, both a complex geography and a scattered population make the costs of extending the national grid prohibitively high. As an alternative, we evaluate the feasibility of an isolated micro-grid, composed by Li-ion batteries and Photovoltaic (PV) panels, for a Bolivian remote community living without access to electricity. We surveyed two remote rural villages to assess the potential electricity consumption patterns of the population once provided access to electricity. We estimated the average demand profile using the tool LoadProGen, and we collected local techno-economic and solar irradiation data of the zone. We employed such data as inputs in a robust linear programming-based optimization tool to determine the capacities of the Li-ion battery bank and the PV array thatminimize the net present cost (NPC) of the system. [less ▲]

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See detailQuantum fidelity of symmetric multipartite states
Neven, Antoine ULg; Mathonet, Pierre ULg; Gühne, Otfried et al

Conference (2017, March)

For two symmetric quantum states one may be interested in maximizing the overlap under local operations applied to one of them. The question arises whether the maximal overlap can be obtained by applying ... [more ▼]

For two symmetric quantum states one may be interested in maximizing the overlap under local operations applied to one of them. The question arises whether the maximal overlap can be obtained by applying the same local operation to each party. We show [1] that for two symmetric multiqubit states and local unitary transformations this is the case; the maximal overlap can be reached by applying the same unitary matrix everywhere. For local invertible operations (stochastic local operations assisted by classical communication equivalence), however, we present counterexamples, demonstrating that considering the same operation everywhere is not enough. [less ▲]

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See detailL’agroécologie, vers la concrétisation d’une utopie ?
Bindelle, Jérôme ULg; De Schutter, Olivier; Dufrêne, Marc ULg et al

Article for general public (2017)

L’agroécologie prône une vision plus responsable de l’agriculture, plus respectueuse de la Terre et de l’Homme. Elle trouve une résonance croissante chez nous, tant dans la société que dans le monde ... [more ▼]

L’agroécologie prône une vision plus responsable de l’agriculture, plus respectueuse de la Terre et de l’Homme. Elle trouve une résonance croissante chez nous, tant dans la société que dans le monde agricole. Quels sont les enjeux de l’agriculture de demain auquel tente de répondre ce modèle alternatif ? Comment creuse-t-il son sillon dans notre paysage agricole ? Quels sont les principes, freins et leviers pour sa mise en oeuvre et sa pérennisation ? [less ▲]

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See detailErnst Mach, psychologue de la forme ?
Zincq, Aurélien ULg

in Philosophie (2017), 133

Detailed reference viewed: 15 (1 ULg)