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See detailLes valeurs et le droit : réflexions à partir du droit des pensions
Detienne, Quentin ULiege

Scientific conference (2017, June 23)

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See detailLatest results with LBTI's Vortex coronagraph: real-time tip/tilt sensing, new data reduction algorithms, and YSO observations
Defrere, Denis ULiege; Hinz, Philip; Absil, Olivier ULiege

Conference (2017, June 23)

Vortex coronagraphs are among the most promising solutions to perform high contrast imaging at small angular separations from bright stars. They enhance the dynamic range at very small inner working angle ... [more ▼]

Vortex coronagraphs are among the most promising solutions to perform high contrast imaging at small angular separations from bright stars. They enhance the dynamic range at very small inner working angle (down to the diffraction limit of the telescope) and provide a clear 360 degree discovery space for high-contrast direct imaging of exoplanets. In 2013, we installed and commissioned an L-band coronagraph in LBTI/LMIRCam and obtained outstanding images of the four planets around HR8799 during the first hours on sky. In this presentation, we will present the results of the latest data reduction performed with the VIP software that is developed at the University of Liège and that features state-of-the-art image processing algorithms inherited from the field of background subtraction in computer vision (including machine learning algorithms and low rank modeling algorithms). We will also present the results obtained with the second L- and M-band coronagraph that was recently installed in LMIRCam to enable binocular Vortex observations. During the first observations (October 2016), we tested and validated a new real-time post-coronagraphic tip-tilt sensing technique (called QACITS) to quickly align each beam on the center of their respective Vortex coronagraph and obtained observations of a young star showing disk features near the resolution limit of each aperture. Finally, we will present some exciting prospects for the Vortex coronagraph that will be installed on VISIR and ELT/METIS. [less ▲]

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See detailDisease detection and management at the wildlife-livestock-human interface
Chaber, Anne-Lise ULiege

Doctoral thesis (2017)

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See detailEffect of N-acetylcysteine on pain in daily life in patients with sickle cell disease: a randomised clinical trial
Sins, JWR; Fijnvandraat, K; Rijneveld, AW et al

in British Journal of Haematology (2017)

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See detailCarrières et biodiversité : l’intérêt d’une évaluation des services écosystémiques
Dufrêne, Marc ULiege; Mercken, Kathleen ULiege

Conference given outside the academic context (2017)

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Peer Reviewed
See detailLa comparaison en Belgique
Delville, Michel ULiege

Conference (2017, June 22)

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Peer Reviewed
See detailUHPLC-ESI-MS/MS quantitation of natural estrogens in aqueous matrices as pyridine-3-sulfonyl derivatives
Glineur, Alex ULiege; Ronkart, Sébastien; Nott, Kathérine et al

Conference (2017, June 22)

Natural estrogens (estrone: E1, 17-β-estradiol : E2, estriol : E3) and synthetic estrogen (17-α-ethinylestradiol: EE2) are powerful endocrine disruptors. They may have deleterious effects on aquatic ... [more ▼]

Natural estrogens (estrone: E1, 17-β-estradiol : E2, estriol : E3) and synthetic estrogen (17-α-ethinylestradiol: EE2) are powerful endocrine disruptors. They may have deleterious effects on aquatic wildlife and also humans even at extremely low concentrations. For this reason, these molecules have been included in a watch list from the European Commission regarding emerging aquatic pollutants. The maximum detection limits are set at 0.035 and 0.40 ng/L for EE2 and E1, E2 respectively. Reaching such low levels of concentration of estrogenic compounds is a challenge, even using state-of-the-art analytical methods. In this study, we developed a UHPLC-ESI-MS/MS method allowing the quantification of E1, E2, E3 and EE2 residues in aqueous matrices. Studies commonly used ESI in negative mode albeit the poor ionization efficiency of native estrogenic compounds in this mode. In this study, the molecules were derivatised using a sulfonyl chloride reagent (pyridine-3-sulfonyl, P-3-S). The resulting response in the positive mode was significantly enhanced. Similarly to other dansyl derivatives, the product ion spectra of the P-3-S derivatives indicate ions originating from the derivatization reagent moiety. Moreover, several other ions were included in the product ion spectra of the P-3-S derivatives. Their specificity was assessed by a qualitative approach implying the analysis of different types of water samples (groundwater, surface water). Some product ions were found to be noticeably better for quantification and confirmation of the analytes. The developed analytical method was validated according to the NF T90-210 norm which is suitable to assess the performances of a method in the water quality field. The limits of quantification were 0.04, 0.05, 0.10 and 0.02 ng/L in groundwater and 0.89, 0.79,4.42 and 0.29 ng/L in surface water for E1, E2, E3 and EE2 respectively. [less ▲]

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See detailImprovement of the ChIP-seq technique for histone posttranslational modifications through enhanced laboratory protocols and data processing methods
Laczik, Miklos ULiege

Doctoral thesis (2017)

Massively parallel sequencing has rapidly become the dominant technique in various omics studies as it provides an unequalled amount of high quality quantitative data for a reasonable cost that is ... [more ▼]

Massively parallel sequencing has rapidly become the dominant technique in various omics studies as it provides an unequalled amount of high quality quantitative data for a reasonable cost that is decreasing ever since the first next generation sequencers appeared. Subsequently, chromatin immunoprecipitation coupled with massively parallel sequencing (ChIP-seq) is the prevailing method of choice for investigating protein–DNA interactions in a genome-wide manner. Bioinformatics tools are also evolving quickly to meet the increasing demands of processing huge amounts of ChIP-seq data and to open the way for novel techniques and insights. However areas still exist that could benefit from improved wet-lab and dry-lab methods. One such area is data visualisation and interpretation; another is the ChIP-seq study of histone posttranslational modifications, especially the research of inactive histone marks which tend to produce diffuse broad enrichments instead of point-source peaks. Achieving proper enrichment and unbiased analysis in such histone mark studies proves to be a great challenge. In this doctoral thesis we show how we addressed these issues on both the level of bioinformatics and the level of sample processing methods. We present our innovative analysis tools we developed to this end, among others a highly customisable, feature rich viewer for next generation sequencing data visualisation, and an analysis pipeline specifically aimed to handle broad enrichments from ChIP-seq studies of (inactive) histone marks. We propose specific software and specific peak calling settings to detect a range of histone modifications accurately, and we describe the way to determine the optimal settings. Along the pipeline we also present a protocol designed to enhance enrichments and facilitate peak detection in broad peak studies typical of inactive histone marks. We demonstrate how this method affects various enrichment types and propose potential applications that could benefit from it. Furthermore we show diverse achievements with the analysis pipeline, including the interpretation of the aforementioned wet-lab method, and the development of an automated ChIP-seq protocol optimised for low cell numbers. [less ▲]

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See detailSouffrir mais: Abstraire, défendre et compatir - Vers une théorie générale du syndicalisme
Toussaint, Sarah ULiege

Master's dissertation (2017)

Quelles sont les représentations sociales des syndicats à l'égard des chômeurs et quels argumentaires développent-ils pour les justifier? En 2016, nous avions abouti à l'élaboration d'une typologie de ... [more ▼]

Quelles sont les représentations sociales des syndicats à l'égard des chômeurs et quels argumentaires développent-ils pour les justifier? En 2016, nous avions abouti à l'élaboration d'une typologie de trois grammaires justificatrices en nous appuyant sur le modèle "De la justification" de Luc Boltanski et Laurent Thévenot. Le travail dont il est question ici se propose de mettre une nouvelle fois à l'épreuve les "cités" intellectuelle, technique et compassionnelle. [less ▲]

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See detailPrehospital identification of trauma patients requiring transfusion: results of a retrospective study evaluating the use of the trauma induced coagulopathy clinical score (TICCS) in 33,385 patients from the TraumaRegister DGU®
TONGLET, Martin ULiege; Lefering, Rolf; Minon, Jean Marc et al

in Acta Chirurgica Belgica (2017)

Background: Identifying trauma patients that need emergent blood product transfusion is crucial. The Trauma Induced Coagulopathy Clinical Score (TICCS) is an easy-to-measure score developed to meet this ... [more ▼]

Background: Identifying trauma patients that need emergent blood product transfusion is crucial. The Trauma Induced Coagulopathy Clinical Score (TICCS) is an easy-to-measure score developed to meet this medical need. We hypothesized that TICCS would assist in identifying patients that need a transfusion in a large cohort of severe trauma patients from the TraumaRegister DGUVR (TR-DGU). Materials and methods: A total of 33,385 severe trauma patients were extracted from the TR-DGU for retrospective analysis. The TICCS was adapted for the registry structure. Blood transfusion was defined as the use of at least one unit of red blood cells (RBC) during acute hospital treatment. Results: With an area under the receiving operating curve (AUC) of 0.700 (95% CI: 0.691–0.709), the TICCS appeared to be moderately discriminant for determining the need for RBC transfusion in the trauma population of the TR-DGU. A TICCS cut-off value of 12 yielded the best trade-off between true positives and false positives. The corresponding positive predictive value and negative predictive values were 48.4% and 89.1%, respectively. Conclusion: This retrospective study confirms that the TICCS is a useful and simple score for discriminating between trauma patients with and without the need for emergent blood product transfusion. [less ▲]

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See detailField border flowering strips as a source of valuable compounds
Paul, Aman ULiege

Doctoral thesis (2017)

Intensification of agricultural practices has caused irreversible damage to environment during the last few years. Several consumers are now deviating towards healthier diets produced from eco-friendly ... [more ▼]

Intensification of agricultural practices has caused irreversible damage to environment during the last few years. Several consumers are now deviating towards healthier diets produced from eco-friendly and sustainable agricultural systems. In this regard, the possibilities of utilizing edible biomass originating from sustainable agricultural practices have gained recent attention. The underutilized edible plants, especially their seeds could be one of the interesting alternates, as some of these seeds are not only nutritious, but could also be produced using sustainable practices. Similarly, edible insects represent another category of biomass which are rich in nutrients and could be produced sustainably. The seeds from underutilized edible plants and the edible insects could be simultaneously harvested using a sustainable agricultural system involving field border flowering strips. Field border flowering strip is a part of agricultural landscape that is reserved for herbaceous vegetation. These strips are popularly grown throughout the world to enhance biodiversity. The main objective of this thesis is to utilize seeds from some of the plants grown as field border flowering strips and insects that find refuge in these plants for the provision of food and health promoting substances. From the literature reviewed in chapter two, it was observed that: (1). Most plants that are grown in field border flowering strips are edible, and their aerial parts have been extensively analyzed for chemical composition. However, there is a scarcity of literature evaluating chemical composition/food utilization of the seeds from plants that are grown as field border flowering strips. So, the primary objective of this thesis is to investigate the nutritional and health promoting potential of the seeds from some plants that are grown in these strips. (2). A number of grasshopper species find refuge in field border flowering strips. Several grasshopper species are considered edible throughout the world and they are interesting source of nutrients. So the secondary objective of this thesis is to screen some edible grasshopper species that are present in field border flowering strips, analyze their nutritional value, and investigate possibilities to establish their commercial rearing for ensuring year-long availability of edible biomass. The research strategy adopted to achieve the objectives of this thesis is mentioned in chapter three. This chapter includes details about the selection of raw materials (both plant seeds and insects), and subsequent analysis. Chapter four contains the detail about the materials and methods used for analysis during this study. Chapter five includes details about the investigations on edible insects. Chorthippus parallelus Zetterstedt species grasshoppers were shortlisted for detailed investigation due to their high densities in field border flowering strips. This insect species was analyzed for proximate composition, amino acid profile, fatty acid profile and mineral profile. Moreover the toxicity of these insects was also evaluated using two different models. Results indicated that these insects could be consumed as an alternate source of proteins (69%) and omega-3 fatty acid rich lipids (10%). Rearing trials done during this study indicates that commercial rearing could be developed to produce sufficient and safe biomass for human consumption. The selection of seeds from three plant species (Achillea millefolium L., Anthriscus sylvestris (L). Hoffm. and Prunella vulgaris L.), for detailed analysis on the basis of lipid content and fatty acid profile has been mentioned in chapter six. Chapter seven, eight and nine include the details about the composition and anti-oxidant activity of A. millefolium, A. sylvestris and P. vulgaris seeds, respectively. Proximate composition, lipid profile, amino acid profile, mineral profile, lignocellulosic profile, phenolic profile and phytate content of the three plant seeds were investigated during this study. Two new phenolic acids were discovered originating from P. vulgaris seeds. These compounds were named amolsamic acid A and amolsamic acid B. Discovery of these compounds was the true highlight of this thesis. All the three plant seeds were found to contain substantial level of total phenolics (0.8-2.6%) and interesting phenolic profiles (dominated by chlorogenic acid, rosmarinic acids and related compounds). Keeping this in mind, the detailed anti-oxidant activity (including anti-radical scavenging, horseradish peroxidase response modulation, cellular anti-oxidant, myeloperoxidase response modulation and anti-lipid peroxidation activity) of their respective seed extracts was also analyzed. Results obtained during this study indicate that A. millefolium, A. sylvestris and P. vulgaris seeds not only contain interesting level of nutrients, but their extracts also exhibit significant anti-radical scavenging, horseradish peroxidase response modulation, cellular anti-oxidant (IC50 values order: P. vulgaris>A. sylvestris>A. millefolium) and myeloperoxidase response modulation activity (IC50 values order: A. sylvestris>A. millefolium>P. vulgaris for both direct and SIEFED assay). The main conclusions (chapter ten) of this PhD dissertation are: (1). C. parallelus insects could be viewed as an alternative source of nutrients to diversify human diets. The preliminary rearing studies done during this study indicate that commercial rearing could be developed for generation of substantial (and safe) biomass to support human consumption. (2). A. millefolium, A. sylvestris and P. vulgaris seeds could be included in food formulations (or consumed as whole) as a source of proteins, lipids, minerals and phenolics. P. vulgaris seeds could also be used for the extraction of two new phenolic constituents (amolsamic acid A and amolsamic acid B). The first investigations involving A. millefolium, A. sylvestris and P. vulgaris seeds realized during this study, indicate that seed extract (or whole seeds) from all three plants could possibly be consumed for the prevention of neutrophil and myeloperoxidase mediated damage in human body. [less ▲]

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See detailFugues d'adolescents : des ruptures en recherche de liens
Glowacz, Fabienne ULiege

Conference given outside the academic context (2017)

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See detailQuality control of insulin formulations: API , protamine and aggregates follow-up
Demelenne, Alice ULiege; Napp, Aurore ULiege; Lamalle, Caroline et al

Poster (2017, June 21)

The prevalence of diabetes is increasing every year and insulin preparations are mostly prescribed for treatment of both type 1 and type 2 diabetes. Since these biopharmaceutical formulations are ... [more ▼]

The prevalence of diabetes is increasing every year and insulin preparations are mostly prescribed for treatment of both type 1 and type 2 diabetes. Since these biopharmaceutical formulations are expensive and require a prescription, they are an important target for counterfeiting in developing countries. Therefore, there is a need for fast and efficient methods for quality control of biopharmaceutical products such as insulin formulations. A fully validated micellar electrokinetic chromatography (MEKC) method was developed for quantification of human insulin and NPH insulin (insulin combined with protamine) in formulations. The BGE used was made of 50 mM ammonium acetate (pH 9.0), 20 mM Sodium dodecyl sulfate and 13 % (v/v) acetonitrile and samples were introduced at the short capillary end allowing a separation of insulin and two major excipients (phenol and m-cresol) within 3 minutes. This method was compared with HPLC method using a mobile phase composed of water with 0.1% formic acid / acetonitrile with 0.1 % formic acid in gradient mode. Secondly a MEKC method was also optimized to analyze protamine peptides in insulin formulations. The major protamine peptides could be separated using a BGE made of 100 mM phosphate buffer (pH 2) with 50 mM Thesit®. This method was used to check conformity of protamine in commercially available formulations. Finally, different approaches were investigated in order to follow insulin aggregation. Indeed, insulin is prone to unfold when submitted to denaturating factors as temperature, ionic strength, agitation and pH. An accumulation of unfolds protein in bloodstream results in a high tendency to aggregate and form amyloid fibrils. A deposit of those fibrils in the subcutaneous tissue leads to a complication called “insulin-derived amyloidosis”. On the other hand, during its production, insulin is often subjected to extreme conditions making aggregation, as well as protein stability, important parameters to be controlled during its quality control. In this study, insulin aggregates were generated after optimization of incubation conditions (pH, temperature, agitation…). Those aggregates were then analyzed by size-exclusion chromatography (SEC) and capillary electrophoresis (CE). We showed that capillary gel electrophoresis (CGE) is a promising technique to analyze covalent aggregates of insulin due to the fact that it separates the aggregates according to their size and not to their size/charge ratio. The use of a laser-induced fluorescence detector was also found attractive to enhance the sensitivity of the method. [less ▲]

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See detailSeasonal Transport in Mars’ Mesosphere revealed by Nitric Oxide Nightglow
Stiepen, Arnaud ULiege

Conference (2017, June 21)

We analyze the ultraviolet nightglow in the atmosphere of Mars through Nitric Oxide (NO) δ and γ bands emissions. On the dayside thermosphere of Mars, solar extreme ultraviolet radiation partly ... [more ▼]

We analyze the ultraviolet nightglow in the atmosphere of Mars through Nitric Oxide (NO) δ and γ bands emissions. On the dayside thermosphere of Mars, solar extreme ultraviolet radiation partly dissociates CO2 and N2 molecules. O(3P) and N(4S) atoms are carried by the day-to-night hemispheric transport. They preferentially descend in the nightside mesosphere in the winter hemisphere, where they can radiatively recombine to form NO(C2Π). The excited molecules promptly relax by emitting photons in the UV δ bands and in the γ bands through cascades via the A2Σ, v’ = 0 state. These emissions are thus indicators of the N and O atom fluxes transported from the dayside to Mars’ nightside and the winter descending circulation pattern from the nightside thermosphere to the mesosphere (e.g. Bertaux et al., 2005 ; Bougher et al., 1990 ; Cox et al., 2008 ; Gagné et al., 2013 ; Gérard et al., 2008 ; Stiepen et al., 2015, 2017). Observations of these emissions have been accumulated on a large dataset of nightside disk images and vertical profiles obtained at the limb by the Imaging Ultraviolet Spectrograph (IUVS, McClintock et al., 2015) instrument when the Mars Atmosphere and Volatile EvolutioN (MAVEN) spacecraft is at its apoapsis and its periapsis phases along its orbit, respectively. We present discussion on the variability in the brightness and altitude and of the emission with season, geographical position and local time and possible interpretation for local and global changes in the mesosphere dynamics. IUVS images and limb scans reveal unexpected complex structure of the emission. The brightest emission is observed close to the winter pole. The emission is also surprisingly more intense in some longitude, revealing possible impact of tides and waves in Mars’ nightside mesosphere. Observations also reveal spots and streaks, indicating irregularities in the wind circulation pattern. The disk images and limb profiles are compared to the LMD-MGCM model (Gonzàlez-Galindo et al., 2009 ; Lopez-Valverde et al., 2011) to focus on the seasonal, local time and geographical influences on the NO Nightglow emission. We will also provide a statistical study of the regions of enhanced brightness (i.e. splotches and streaks) and discuss possible interpretation from the comparison to the GCM. [less ▲]

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See detailL’art de gouverner les régions par les finances publiques
Piron, Damien ULiege

Conference (2017, June 21)

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See detailConclusions of the 6th European Drying Conference
Léonard, Angélique ULiege

Conference (2017, June 21)

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See detailImpact of conditioning on sludge internal structure evolution during drying
Fraikin, Laurent ULiege; Pambou, Yvon Bert; Salmon, Thierry ULiege et al

Conference (2017, June 20)

This paper investigated the influence of dual conditioning of waste water treatment sludge on the evolution of sludge internal structure during convective drying. Results showed clearly an impact of ... [more ▼]

This paper investigated the influence of dual conditioning of waste water treatment sludge on the evolution of sludge internal structure during convective drying. Results showed clearly an impact of chemical conditioners on the evolution of the created porosity. For sludge flocculated by single conditioning, a centered porosity delimiting by external crust was shown, while for dual conditioning, a porosity structured in lamellae, was identified. [less ▲]

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See detailDiametral dimension(s) and prominent bounded sets
Demeulenaere, Loïc ULiege

Conference (2017, June 20)

The classical diametral dimension (Bessaga, Mityagin, Pelczynski, Rolewicz), denoted by "Delta", is a topological invariant which can be used to characterize Schwartz and nuclear locally convex spaces ... [more ▼]

The classical diametral dimension (Bessaga, Mityagin, Pelczynski, Rolewicz), denoted by "Delta", is a topological invariant which can be used to characterize Schwartz and nuclear locally convex spaces. Mityagin also introduced a variant of this diametral dimension, denoted by "Delta_b", using bounded sets in its definition, contrary to "Delta". In this talk, we present some conditions assuring the equality of these two diametral dimensions for Fréchet spaces. Among these conditions, there is the notion of existence of prominent bounded sets, due to Terzioglu. In fact, it appears that the existence of prominent sets is implied by the property "Omega Bar" of Vogt and Wagner. Finally, we describe a construction which gives Schwartz and nuclear non-Fréchet spaces E verifying "Delta_b(E) = \Delta(E)". [less ▲]

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See detailLe pouvoir politique des juges
Grandjean, Geoffrey ULiege

Conference given outside the academic context (2017)

Notre société est marquée par un phénomène de judiciarisation qui voit une emprise croissante des juges dans les systèmes politiques et sociaux. Certains représentants n'hésitent d'ailleurs plus à ... [more ▼]

Notre société est marquée par un phénomène de judiciarisation qui voit une emprise croissante des juges dans les systèmes politiques et sociaux. Certains représentants n'hésitent d'ailleurs plus à fustiger le « gouvernement des juges ». Quels pouvoirs les juges exercent-ils dans nos systèmes démocratiques ? Pour répondre à cette question, il est proposé de présenter les trois grandes fonctions politiques des juges : 1) la production de normes, 2) l'arbitrage de valeurs morales et 3) la stabilisation du système politique. Ces trois fonctions sont autant de déclinaisons du pouvoir politique des juges qui concrétisent un nouvel équilibre entre les pouvoirs. De nombreuses décisions de jurisprudence illustrent le propos. [less ▲]

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