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See detailUtility of removing cholesterol from an industrial by-product- Buttermilk powder
Malik, Priyanka ULg; Danthine, Sabine ULg; Paul, Aman ULg et al

Poster (2015, January 30)

Almost 16% of milk produced in European Union is being converted to butter. Buttermilk, is a low cost by-product from butter manufacture and available in large quantities but has been considered ... [more ▼]

Almost 16% of milk produced in European Union is being converted to butter. Buttermilk, is a low cost by-product from butter manufacture and available in large quantities but has been considered invaluable for many years. When cream is churned to butter the stable oil in water emulsion is destabilized and milk fat globule membrane (MFGM) is disrupted which is released in buttermilk. Thus, over the last two decades it has gained considerable attention due to its specific composition (proteins and polar lipids) attributed towards the MFGM. In account to this composition of buttermilk powder, it finds application in food industry as a natural stabilizing and emulsifying agent. It also finds application as a source of total solids and is commercially being added upto 10% in many food products. However, it has limitation in such applications due to the presence of cholesterol (approx. 80mg/100g). Cholesterol is also an integral part of MFGM and is released into buttermilk with MFGM. The most suitable techniques to remove cholesterol from buttermilk powder are using β-cyclodextrin and cholesterol oxidase enzyme. To valorize this abundantly produced industrial by-product of butter industry and increase its application in health food industry, as well as extend its application in pharmaceutical industry and preparation of liposomes, it is important to remove cholesterol from buttermilk. [less ▲]

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See detailNitrous oxide flux measurement with a closed chamber system : data treatment
Regaert, Donat ULg; Moureaux, Christine ULg; Heinesch, Bernard ULg et al

Poster (2015, January 30)

Nitrous oxide flux estimation from concentration measurements with a closed chamber system. Statistical data treatment to sort between relevant/irrelevant fluxes.

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See detailWildflower strips for crop protection: What do we know ? What should we know ?
Hatt, Séverin ULg; Uyttenbroeck, Roel ULg; Bodson, Bernard ULg et al

Poster (2015, January 30)

Wildflower strips (WFS) are known to support the conservation of a large diversity of insects and thus natural enemies (i.e. predators and parasitoids) that can control pests. However, the conclusions of ... [more ▼]

Wildflower strips (WFS) are known to support the conservation of a large diversity of insects and thus natural enemies (i.e. predators and parasitoids) that can control pests. However, the conclusions of studies looking at the efficiency of WFS to control pests are not unanimous. Indeed, the enhancement of pest control seems to depend on (1) the ability of flowers to attract the natural enemies at the right moment and (2) the capacity of natural enemies to migrate into the adjacent crops to attack pests. Therefore, constituting appropriate flower mixes may be an essential lever to enhance the efficiency of pest control. In this context, using functional diversity is promising. To our knowledge, few studies have tested the impact of the functional diversity of a flower mix on insect abundance and diversity and the control of pests. Through this contribution, the insect diversity and abundance found to be associated with the different kinds of WFS and management applied will be discussed, as well as the further research needed. [less ▲]

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See detailLinking bees and flowers: mutualistic interaction networks to study ecosystem functioning
Uyttenbroeck, Roel ULg; Monty, Arnaud ULg

Poster (2015, January 30)

In recent years, much attention is going to pollinators, as they are important for the increasing food production while being threatened by agricultural intensification and other environmental drivers ... [more ▼]

In recent years, much attention is going to pollinators, as they are important for the increasing food production while being threatened by agricultural intensification and other environmental drivers. While honeybees (Apis mellifera L.) are generalist according to their flower preferences, wild pollinators’ flower preferences can go from generalist to very specialist. The same goes for plant species, which can be generalist or specialist in the pollinator species they need to get pollinated. The combination of a set of generalist and specialist plant species with a pollinator community consisting of generalists and specialists in a certain habitat, results in an interaction network between pollinators and the visited plant flowers. Studying this mutualistic interaction network is a time-consuming method, but can provide valuable data to calculate indicators of ecosystem functioning. One of the important conclusions that came already out of studies applying this method is that pollination systems are often more generalized than thought based on pollination syndromes. This poster will explore the method of studying mutualistic interaction networks with a focus on the sampling protocol, the indicators that can be derived from the data and their meaning. [less ▲]

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See detailLessons from the field: Negotiating Transnational Gender, Class and Race Positions as a Researcher Working on Andean Domestic Workers in Brussels
Vivas Romero, Maria ULg

Scientific conference (2015, January 30)

This paper shows the links between migrant transnationalism and the methodological debates concerning the researcher’s positionality and self-reflexivity, which have not been systematically considered in ... [more ▼]

This paper shows the links between migrant transnationalism and the methodological debates concerning the researcher’s positionality and self-reflexivity, which have not been systematically considered in transnational studies. Drawing from my experiences as a transnational female migrant conducting fieldwork among Andean domestic workers in the city of Brussels; I intent to contribute to the conceptualization of positionality by reflecting on the effect of the researcher’s transnational gender, class and race positions in qualitative migration research. Firstly, I address the conflicts that arose from my positioning as an “insider” that initially granted me a social proximity with my participants but later raised awareness on the existent social boundaries between us and temporarily blocked my access to the field. Secondly, I will highlight the methodological strategies through, which I worked around the existent boundaries of power relations and eventually gained access to the field, meaning: A- Constantly, engaging in self-reflexivity and questioning my “insider/outsider positioning, B- Consciously and unconsciously negotiating my class, gender and race positioning’s in order to re-draw the boundaries in the relationship between the participants and the researcher, C- Relying on my experiences as a transnational migrant in order to grasp the meaning of their practices, which guided me to avoid my spontaneous interpretations and reconsider those that my participants gave me . Finally, I will argue that both mobilizing one’s personal background and negotiating our intersecting transnational positionalities during fieldwork constitute valuable methodological resources that might lead us to do research that is produced through more symmetrical power relations. [less ▲]

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See detailEvaluation et gamification - Transformer un quiz de chimie en un mini-jeu
le Maire, Nathalie ULg; Fauconnier, Marie-Laure ULg; Colaux, Catherine ULg et al

Conference (2015, January 30)

Le recours à des questionnaires à choix multiples pour l’évaluation des étudiants est une pratique courante à l’université. Cette communication décrit et analyse une expérimentation contrôlée visant à ... [more ▼]

Le recours à des questionnaires à choix multiples pour l’évaluation des étudiants est une pratique courante à l’université. Cette communication décrit et analyse une expérimentation contrôlée visant à explorer les effets de la présentation d’un QCM formatif sous la forme d’un jeu. L’étude s’inscrit ainsi dans les recherches actuelles sur les avantages et les limites pédagogiques de la « gamification », à savoir la transposition des principes issus de l’univers du jeu au domaine de l’éducation (Johnson, Adams Becker, Estrada, & Freeman, 2014). La présente recherche est effectuée dans le cadre d’un doctorat dédié spécifiquement à la gamification d’évaluations formatives. L’étude repose sur la mise à disposition de plusieurs versions d’un quiz portant sur un chapitre d’un cours de chimie générale en première année d’université. Le dispositif expérimental prévoit un enrichissement graduel de la gamification selon quatre prototypes conçus après identification et extraction de schèmes visant à stimuler et maintenir la motivation et la performance dans des jeux actuels et populaires tels que Candy Crush, Angry Birds ou Hay Day. Située à l’entrée du doctorat, cette étude vise, outre la collecte de données quant à l’utilisabilité du mini-jeu proposé, à mettre en lumière ses effets sur l’état de flow dans le contexte des edugames et contribuera à la validation de la traduction française de l’échelle d’EGameFlow (Fu, Su, & Yu, 2009). [less ▲]

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See detailInfluence of cover crop management on sugar beet production
Hiel, Marie-Pierre ULg; Bodson, Bernard ULg

Poster (2015, January 30)

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See detailActivation of the calcium-sensing receptor before renal ischemia/reperfusion exacerbates kidney injury
WEEKERS, Laurent ULg; De Tullio, Pascal ULg; BOVY, Christophe ULg et al

in American Journal of Translational Research (2015), 7(1), 128-138

Activation of the calcium-sensing receptor (CaSR) by ischemia/reperfusion (I/R) favours apoptosis in cardiomyocytes, hepatocytes and neurons. Its role in renal I/R is unknown. We investigated the impact ... [more ▼]

Activation of the calcium-sensing receptor (CaSR) by ischemia/reperfusion (I/R) favours apoptosis in cardiomyocytes, hepatocytes and neurons. Its role in renal I/R is unknown. We investigated the impact of pharmacological preactivation of the CaSR on kidney structure and function in a murine model of bilateral renal 30-min ischemia and 48-hour reperfusion, and in a 6-year cohort of kidney transplant recipients (KTR). C57BL/6J mice were administered daily with CaSR agonist, R-568, or with vehicle for 48 hours. Evaluation of serum urea and creatinine levels, renal histology and urine metabolome by nuclear magnetic resonance showed that R-568 was not nephrotoxic per se. Following I/R, serum urea and creatinine levels increased higher in R-568-treated animals than in controls. Jablonski’s score was significantly greater in R-568-treated kidneys, which showed a higher rate of cell proliferation and apoptosis in comparison to controls. Next, we retrospectively identified 36 patients (10.7% of our cohort) who were treated by CaSR agonist, cinacalcet, at the time of kidney transplantation (KTx). After matching these to 61 KTR upon type of donor, cold ischemic time, residual diuresis, and donor age, we observed that delayed graft function, i.e. need for dialysis in the first week after KTx, occurred in 42 and 23% of cinacalcet-treated and control groups, respectively (p≤0.05). These data suggest that pharmacological preactivation of the CaSR before renal I/R exacerbates kidney injury. [less ▲]

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See detailInvestigating the Effects of Plant Root Exudates on PAHs Bioavailability to Soil Microorganisms in Contaminated Brownfields : Research Methodology.
Davin, Marie ULg; Lognay, Georges ULg; Fauconnier, Marie-Laure ULg et al

Poster (2015, January 30)

As a result of heavy industrial past activities, an estimated 6,000 brownfields require remediation in Wallonia. This number rises to over 3.5 million in Europe. Polycyclic Aromatic Hydrocarbons (PAHs ... [more ▼]

As a result of heavy industrial past activities, an estimated 6,000 brownfields require remediation in Wallonia. This number rises to over 3.5 million in Europe. Polycyclic Aromatic Hydrocarbons (PAHs) represent 17% of treated pollutants in Wallonia (Aldric et al., 2011). Current remediation techniques are rather expensive and technically demanding (Megharaj et al., 2011). Based on the observation that PAHs soil content decreases in the presence of plants (Cheema et al., 2010), the PhD aims at developing alternative PAHs remediation techniques in brownfields. It is articulated around three research axes. The first axis focusses on plant exudates and how they may improve PAHs bioavailability to soil microorganisms and enhance their degradation. This will be investigated by (i) characterizing several contaminated soils (physico-chemical parameters) and PAH content and factors of bioavailability, (ii) selecting a plant model and collecting root exudates, and (iii) evaluating the effects of exudates on PAHs bioavailability. The objective of the second axis is to evaluate the effects of plant exudates on PAHs degrading microorganisms by (i) comparing PAHs biodegradation in the presence/absence of exudates and (ii) assessing the potential toxic effects of exudate compounds on the microbial communities. The aim of the third axis is to study plant-pollutants interactions by (i) establishing the plant tolerance to several contamination levels and (ii) following PAHs bioavailability when facing real exudation rates, on the field. [less ▲]

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See detailLa participation : pour quoi faire ?
Mormont, Marc ULg

Conference given outside the academic context (2015)

La participation du public aux politiques environnementales peut prendre de multiples formes. Mais elle relève aussi de justifications différentes qui correspondent à des formes d'engagement très ... [more ▼]

La participation du public aux politiques environnementales peut prendre de multiples formes. Mais elle relève aussi de justifications différentes qui correspondent à des formes d'engagement très différentes. La contribution de la participation est légitime au plan légal et politique, mais son instrumentation est faible. L'auteur insiste sur deux critères cruciaux d'évaluation ; la délibération et les niveaux d'engagement des publics. [less ▲]

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See detailNumerical modeling of the long term behavior of Municipal Solid Waste in a landfill
Hubert, Julien ULg; Collin, Frédéric ULg

Scientific conference (2015, January 30)

Detailed reference viewed: 25 (7 ULg)
See detailAzacytidine augments regulatory T cells and prevents experimental xenogeneic graft-versus-host disease.
Ehx, Grégory ULg

Conference (2015, January 30)

The demethylating agent 5-azacytidine (AZA) has proven its efficiency in myeloid malignancies treatment. Recently, several studies have shown that AZA also presents an immunomodulatory activity, mainly ... [more ▼]

The demethylating agent 5-azacytidine (AZA) has proven its efficiency in myeloid malignancies treatment. Recently, several studies have shown that AZA also presents an immunomodulatory activity, mainly through the induction of regulatory T cells (Treg) differentiation from conventional T cells. Treg promotion is a promising treatment option for graft-versus-host disease (GVHD) following hematopoietic stem cell transplantation. In this way, AZA has been shown to reduce GVHD in mouse-to-mouse transplantation models. However, the impact of AZA on human GVHD remains elusive as well as its effects on human Treg and on the other T cell subsets in vivo. Here we report AZA impact on GVHD in a xeno-transplantation model of the disease and describe the overall effects of the drug on human T cells in vivo. When administered to NSG mice receiving 2.107 human PBMC intravenously, AZA successfully ameliorated both survival (p < 0.001) and GVHD score. Flow cytometry analyses showed that this improvement may be mediated through an anti-proliferative effect of AZA on human T cells while these cells presented an increased activation state. We also observed a significant decrease of Th1 and 2 differentiation, assessed by RT-qPCR and cytokines secretion assays, and a reduced production of granzyme B and perforin 1 by cytotoxic T cells. As expected, AZA dramatically augmented Treg frequency while we could also observe a promotion of their IL-2 mediated proliferation over conventional T cells. Interestingly, Th17 frequency was not affected by AZA, therefore increasing the balance Treg/Th17. Finally our data suggest that AZA-induced Treg may have an improved suppressive activity and present long-term stability in vivo. Altogether our findings suggest that AZA may be a promising treatment option for GVHD in the clinical setting. [less ▲]

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See detailAzacytidine prevents experimental sclerodermic chronic graft-versus-host disease
Fransolet, Gilles ULg; Ehx, Grégory ULg; SOMJA, Joan ULg et al

Poster (2015, January 30)

Introduction: Graft-versus-host disease (GVHD) remains one major complication of allogeneic hematopoietic stem cell transplantation (HSCT). Following unmanipulated peripheral-blood stem cell ... [more ▼]

Introduction: Graft-versus-host disease (GVHD) remains one major complication of allogeneic hematopoietic stem cell transplantation (HSCT). Following unmanipulated peripheral-blood stem cell transplantation, 60% of the patients experience chronic GVHD while approximately 15% of them develop a sclerodermic form of chronic GVHD characterized by multiple organ fibrosis and loss of skin elasticity. Regulatory T cells (Tregs) play a pivotal role in the pathology of chronic GVHD by inhibiting alloreactive conventional T cells. Several studies have shown the hypomethylating agent Azacytidine (Aza) can demethylate the master transcription factor of Treg (Forkhead box protein 3 factor, FoxP3), thus promoting Treg differentiation of conventional T cells. This work investigates the impact of Aza in a classical murine model of sclerodermic chronic GVHD (B10.D2  BALB/cJ). Methods: Lethally irradiated BALB/cJ recipient mice were injected with 107 bone marrow cells + 7.107splenocytes from B10.D2 donor mice. Recipients were treated with subcutaneous injections of Aza at the dose of 0,5 or 2 mg/kg every two days from day 10 to 30 following transplantation. Mice GVHD was evaluated with five criteria (weight loss, activity, fibrosis, hair loss and mice posture; 0-1-2 points/criteria). Mice were sacrificed at a score of 8/10 (or > 20% weight loss). Results: Mice treated with Aza 0.5 mg/kg (n = 14) or 2 mg/kg (n = 17) had significant lower clinical scores compared to control ones (n = 15) after treatment. FACS analysis showed a higher proportion of Treg among CD4+ T cells in the blood of Aza 2 mg/kg mice than in control mice (P = 0.047), as well as a higher percentage of Tregs expressing the KI67 proliferative marker on the same day (P = 0.0005). Finally, analyses of the cellular blood components with Cell-dyn demonstrated that Aza 2 mg/kg treated mice were significantly lymphopenic as compared to control mice (P = 0.05). Conclusion : Aza prevented sclerodermic GVHD in this classical murine model of chronic GVHD. [less ▲]

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See detailFéminiser la voix
Morsomme, Dominique ULg; Remacle, Angélique ULg

Conference (2015, January 29)

Parlée ou chantée, notre voix révèle qui nous sommes. Elle participe à notre identité et véhicule nos émotions (ref. Revis. J). Dans cet exposé, nous présenterons la voix du point de vue du genre. En ... [more ▼]

Parlée ou chantée, notre voix révèle qui nous sommes. Elle participe à notre identité et véhicule nos émotions (ref. Revis. J). Dans cet exposé, nous présenterons la voix du point de vue du genre. En effet, l’Unité Logopédie de la Voix (www.logopedie-voix.be) s’intéresse entre autres à la féminisation vocale chez les personnes transidentitaires, en processus de changement de genre. Ces personnes ne s’identifiant pas au sexe et au genre qui leur ont été attribués à la naissance, elles éprouvent un désir profond de vivre comme une personne du sexe opposé à leur sexe biologique. Lors du processus de transition où ont lieu les transformations nécessaires au changement de genre, la logopédie a pour but d’améliorer la cohérence entre l’apparence de la personne et sa voix. Ainsi, notre recherche se focalise sur ce qui distingue la voix féminine de la voix masculine. Nous visons à développer des stratégies de féminisation vocale, portant sur des paramètres tels que la fréquence fondamentale de la voix (grave ou aigüe), les contours intonatifs, le niveau d’amplitude de certains formants, le débit de parole, son rythme, ou encore le vocabulaire choisi. Féminiser une voix, c’est chercher à comprendre ce qui permet d’en identifier le genre, mais également participer à la construction de l’identité de la personne à part entière. [less ▲]

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See detailCompétences complexes en Anatomie : évaluation par QCM
Bonnet, Pierre ULg; Ernst, Denise ULg; Defaweux, Valérie ULg

Conference (2015, January 29)

L’apprentissage de l’Anatomie débute en faculté de Médecine par un cours d’introduction dispensé à toutes les premières années de baccalauréat de la faculté: Médecins, Kinésithérapeutes, Sciences de la ... [more ▼]

L’apprentissage de l’Anatomie débute en faculté de Médecine par un cours d’introduction dispensé à toutes les premières années de baccalauréat de la faculté: Médecins, Kinésithérapeutes, Sciences de la Motricité, Dentistes, Pharmaciens et Sciences Biomédicales. Outre les objectifs d’apprentissage spécifiques anatomiques, le dispositif d’enseignement vise à atteindre des objectifs transversaux : sens de l’observation, capacité de description, vision dans l’espace. Ces capacités sont entraînées au travers d’un apprentissage mixte (présentiel et on-line), tant pour les aspects théoriques que pratiques, faisant appel à la réalisation de schémas et à l’analyse d’images et de vidéos. Les cohortes d’étudiants sont importantes (1500 étudiants) ce qui rend obligatoire le recours à des moyens d’évaluation automatisés : Questions à Choix Multiples et feuille de réponse à lecture optique. Dans ce contexte, nous avons développé des énoncés de question à choix multiples basés soit sur des notions théoriques, soit sur l’analyse de schémas simples soit sur des exercices à résoudre. Afin de parfaire l’alignement pédagogique, des vidéos explicatives portant sur la résolution d’exercices type sont disponibles sur le cours on-line. Un sondage réalisé auprès des étudiants et une analyse de l’utilisation des outils du cours confirment la cohérence du dispositif. Les résultats globaux peuvent être ventilés en 3 cotes suivant le type de questions utilisées : théorie, analyse, exercice. Cette information renseigne l’étudiant sur le niveau et le type de compétences acquises et devrait permettre de construire des évaluations mieux adaptées à la finalité différente des 6 sections concernées par une pondération variable des types de question. [less ▲]

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See detailGrille de correction: déclinaisons de la perception et des réalisations
Bonnet, Pierre ULg; Delvenne, Philippe ULg; Defaweux, Valérie ULg et al

Conference (2015, January 29)

Un outil disponible on-line pour la correction des questions ouvertes à réponse courte ou longue (QROC et QROL) est utilisé depuis plusieurs années au sein de l’ULg. Les copies réponse des étudiants sont ... [more ▼]

Un outil disponible on-line pour la correction des questions ouvertes à réponse courte ou longue (QROC et QROL) est utilisé depuis plusieurs années au sein de l’ULg. Les copies réponse des étudiants sont scannées et corrigées sur un écran d’ordinateur. L’enseignant fournit l’énoncé de la question et une grille de correction suivant un canevas prédéfini. La correction évalue une série de critères regroupés par 4 à l’écran. Chaque critère défini est évalué au moyen d’une échelle horizontale allant de 0 à 100% suivant que le critère n’est pas rencontré (0%) ou qu’il est totalement développé (100%) par l’étudiant. L’enseignant peut graduer cette échelle en y indiquant des repères (indices). La pondération des différents critères est définie à priori ou à posteriori. Sur cette base de travail commune, nous analysons la façon dont cette grille est utilisée pour corriger des questions relatives à des matières médicales différentes enseignées par différents professeurs : histologie, anatomie, physiologie et anatomopathologie. L’analyse fait ressortir que malgré un canevas commun, les modalités de correction sont variables ce qui témoigne de la différence existant entre les matières, les compétences évaluées et, probablement, la sensibilité des enseignants. A l’inverse, ceci démontre la souplesse de l’outil adaptable à des types d’évaluations différents. [less ▲]

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See detailValidating the use of an ultra-performance liquid chromatography with tandem mass spectrometry method to quantify equol in cow's milk
Daems, Frédéric ULg; Jasselette, Christophe; Romnee, Jean-Michel et al

in Dairy Science & Technology (2015)

Cow’s milk can be used as a potential source of equol in the human diet. In order to study human intake, however, it is necessary to develop a reliable and sensitive analytical method. This paper reports ... [more ▼]

Cow’s milk can be used as a potential source of equol in the human diet. In order to study human intake, however, it is necessary to develop a reliable and sensitive analytical method. This paper reports on the validation of an analytical method using ultra-performance liquid chromatography coupled with a tandem mass spectrometry detector to quantify the equol in commercial milks (raw, whole, semi-skimmed, and skimmed milk). The equol was initially released using enzymatic hydrolysis, and it was then extracted using a double liquid/liquid extraction. The analytical method produced a linear calibration curve with a high correlation coefficient (R 2 ≥ 0.996) between 5 and 1,000 ng.mL−1. Good intra- and inter-day precision (≤5.3% and ≤ 5.2%, respectively) and accuracy (≤8.6%) were achieved. The recovery rate differed slightly among the different types of milk, ranging between 60.6 ± 1.09% and 82.3 ± 5.21%. Good method repeatability was observed (<15%). There was neither matrix effect nor carry-over effect, and the sample extracts were stable for at least 7 days of storage at −21 °C and 5 °C. The method proved to be specific, sensitive, precise, and accurate and was used for the first time to quantify the equol content in Belgian commercial cow’s milk. In all the samples analyzed, equol was present at a concentration ≥10 ng.mL−1 and had a significantly higher content in organic than in conventional milk. The study also found that the mean concentrations of equol were similar for each type of commercial conventional cow’s milk. [less ▲]

Detailed reference viewed: 31 (9 ULg)