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See detailCarnet de pédiatrie: néphrologie de l'enfant
Battisti, Oreste ULg

Learning material (2013)

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See detailThe scent of inbreeding: male sex pheromones betray inbred males
van Bergen, Erik; Brakefield, Paul M.; Heuskin, Stéphanie ULg et al

in Proceedings of the Royal Society B : Biological Sciences (2013)

Inbreeding depression results from mating among genetically related individuals and impairs reproductive success. The decrease in male mating success is usually attributed to an impact on multiple fitness ... [more ▼]

Inbreeding depression results from mating among genetically related individuals and impairs reproductive success. The decrease in male mating success is usually attributed to an impact on multiple fitness-related traits that reduce the general condition of inbred males. Here, we find that the production of the male sex pheromone is reduced significantly by inbreeding in the butterfly Bicyclus anynana. Other traits indicative of the general condition, including flight performance, are also negatively affected in male butterflies by inbreeding. Yet, we unambiguously show that only the production of male pheromones affects mating success. Thus, this pheromone signal informs females about the inbreeding status of their mating partners. We also identify the specific chemical component, hexadecanal, probably responsible for the decrease in male mating success. Our results advocate giving increased attention to olfactory communication as a major causal factor of mate-choice decisions and sexual selection. [less ▲]

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See detailA spectroscopic investigation of the O-type star population in four Cygnus OB associations. I. Determination of the binary fraction
Mahy, L.; Rauw, Grégor ULg; De Becker, Michaël ULg et al

in Astronomy and Astrophysics (2013), 550

Establishing the multiplicity of O-type stars is the first step towards accurately determining their stellar parameters. Moreover, the distribution of the orbital parameters provides observational clues ... [more ▼]

Establishing the multiplicity of O-type stars is the first step towards accurately determining their stellar parameters. Moreover, the distribution of the orbital parameters provides observational clues to the way that O-type stars form and to the interactions during their evolution. Our objective is to constrain the multiplicity of a sample of O-type stars belonging to poorly investigated OB associations in the Cygnus complex and for the first time to provide orbital parameters for binaries identified in our sample. Such information is relevant to addressing the issue of the binarity in the context of O-type star formation scenarios. We performed a long-term pectroscopic survey of nineteen O-type stars. We searched for radial velocity variations to unveil binaries on timescales from a few days up to a few years, on the basis of a large set of optical spectra. We confirm the binarity for four objects: HD193443, HD228989, HD229234 and HD194649. We derive for the first time the orbital solutions of three systems, and we confirm the values of the fourth, showing that these four systems all have orbital periods shorter than 10 days. Besides these results, we also detect several objects that show non-periodic line profile variations in some of their spectral lines. These variations mainly occur in the spectral lines, that are generally affected by the stellar wind and are not likely to be related to binarity. The minimal binary fraction in our sample is estimated to be 21%, but it varies from one OB association to the next. Indeed, 3 O stars of our sample out of 9 (33%) belonging to CygOB1 are binary systems, 0% (0 out of 4) in CygOB3, 0% (0 out of 3) in CygOB8, and 33% (1 out of 3) in CygOB9. Our spectroscopic investigation also stresses the absence of long-period systems among the stars in our sample. This result contrasts with the case of the O-type stellar population in NGC 2244 among which no object showed radial velocity variations on short timescales. However, we show that it is probably an effect of the sample and that this difference does not a priori suggest a somewhat different star forming process in these two environments. [less ▲]

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See detailLa clinique psychologique et logopédique : une approche intégrée du patient
Maillart, Christelle ULg; Catale, Corinne ULg

Conference given outside the academic context (2013)

Le travail en équipe multidisciplinaire permet d’envisager l’évaluation et la prise en charge d’un patient de manière intégrée : illustration de la collaboration entre neuropsychologue et logopède dans le ... [more ▼]

Le travail en équipe multidisciplinaire permet d’envisager l’évaluation et la prise en charge d’un patient de manière intégrée : illustration de la collaboration entre neuropsychologue et logopède dans le cadre de suivis d’enfants et d’adolescents. [less ▲]

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See detailOccurrence of Harmonia axyridis (Coleoptera: Coccinellidae) in field crops
Vandereycken, Axel ULg; Brostaux, Yves ULg; Joie, Emilie ULg et al

in European Journal of Entomology (2013), 110(2),

Abstract. The Multicoloured Asian Ladybird, Harmonia axyridis Pallas (Coleoptera: Coccinellidae) is known to thrive principally in shrubby and arboreal habitats. This study focuses on the occurrence of ... [more ▼]

Abstract. The Multicoloured Asian Ladybird, Harmonia axyridis Pallas (Coleoptera: Coccinellidae) is known to thrive principally in shrubby and arboreal habitats. This study focuses on the occurrence of this exotic species and its seasonal abundance in various field crops. The abundance of adults, larvae and pupae of H. axyridis was evaluated over a three-year period, from 2009 to 2011, in four important agronomical crops (wheat, corn, broad bean and potato) in Belgium. From May to September, 48 1-m² quadrats were visually inspected in each of the fields sampled on several farms every seven days. H. axyridis colonized and reproduced in all of the four crops studied, with the largest numbers recorded in corn and broad bean crops. Larvae and adults of H. axyridis were recorded mainly in corn and to a much less extent in wheat and potato crops. From 2009 to 2011, the mean weekly abundance of H. ayxridis remained constant except in corn crops, where the recorded densities of all the immature stages and adults were higher in 2011 than in 2009. The population dynamics of aphids and H. axyridis are well described by a symmetric logistic function (S-shape) of cumulative population size. H. axyridis was not always recorded where aphids were abundant, e.g. aphids were abundant on wheat where no H. axyridis were recorded. H. axyridis start reproducing after the peak in aphid population, suggesting that H. axyridis is able to complete its development by feeding on alternative prey such as larvae and pupae of the same and other species of ladybird and other aphidophagous species. H. axyridis is often considered to be bivoltine but it only completes one generation per year in field crops. The second generation generally develops late in the season in other habitats. [less ▲]

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See detailFollow the bouncing ball: global results on tracking and state estimation with impacts
Forni, Fulvio ULg; Teel, Andrew; Zaccarian, Luca

in IEEE Transactions on Automatic Control (2013)

In this paper we formulate tracking and state- estimation problems of a translating mass in a polyhedral billiard as a stabilization problem for a suitable set. Due to the discontinuous trajectories ... [more ▼]

In this paper we formulate tracking and state- estimation problems of a translating mass in a polyhedral billiard as a stabilization problem for a suitable set. Due to the discontinuous trajectories arising from the impacts, we use hybrid systems stability analysis tools to establish the results. Using a novel concept of mirrored images of the target mass we prove that 1) a tracking control algorithm, and 2) an observer algorithm guarantee global exponential stability results for specific classes of polyhedral billiards, including rectangles. Moreover, we combine these two algorithms within dynamic controllers that guarantee global output feedback tracking. The results are illustrated via simulations. [less ▲]

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See detailLyapunov-based sufficient conditions for exponential stability in hybrid systems
Teel, Andrew; Forni, Fulvio ULg; Zaccarian, Luca

in IEEE Transactions on Automatic Control (2013)

Lyapunov-based sufficient conditions for exponential stability in hybrid systems are presented. The focus is on converting non-strict Lyapunov conditions, having certain observability properties, into ... [more ▼]

Lyapunov-based sufficient conditions for exponential stability in hybrid systems are presented. The focus is on converting non-strict Lyapunov conditions, having certain observability properties, into strict Lyapunov conditions for exponential stability. Both local and global results are considered. The utility of the results is illustrated through an example. [less ▲]

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See detailA note on the item information function of the four-parameter logistic model
Magis, David ULg

in Applied Psychological Measurement (2013), 37

This paper focuses on four-parameter logistic (4PL) model (Barton & Lord, 1981) as an extension of the usual three-parameter logistic (3PL) model with an upper asymptote possibly different from one. For a ... [more ▼]

This paper focuses on four-parameter logistic (4PL) model (Barton & Lord, 1981) as an extension of the usual three-parameter logistic (3PL) model with an upper asymptote possibly different from one. For a given item with fixed item parameters, Lord (1980) derived the value of the latent ability level that maximizes the item information function under the 3PL model. The purpose of this paper is to extend this result to the 4PL model. A generic and algebraic method is developed for that purpose. The result is practically illustrated by an example and several potential applications of this result are outlined. [less ▲]

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See detailAnopheles species associations in Southeast Asia: indicator species and environmental influences
Obsomer, Valérie; Dufrêne, Marc ULg; Defourny, Pierre et al

in Parasites & Vectors (2013), 6

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See detailIdentification and functional characterization of a novel αlpha-conotoxin (EIIA) from Conus ermineus
Quinton, Loïc ULg; Servent, Denis; Girard, Emmanuelle et al

in Analytical and Bioanalytical Chemistry (2013), 405

Nicotinic acetylcholine receptors (nAChRs) are one of the most important families in the ligand-gated ion channel superfamily due to their involvement in primordial brain functions and in several ... [more ▼]

Nicotinic acetylcholine receptors (nAChRs) are one of the most important families in the ligand-gated ion channel superfamily due to their involvement in primordial brain functions and in several neurodegenerative pathologies. The discovery of new ligands which can bind with high affinity and selectivity to nAChR subtypes is of prime interest in order to study these receptors and to potentially discover new drugs for treating various pathologies. Predatory cone snails of the genus Conus hunt their prey using venoms containing a large number of small, highly structured peptides called conotoxins. Conotoxins are classified in different structural families and target a large panel of receptors and ion channels. Interestingly, nAChRs represent the only subgroup for which Conus has developed seven distinct families of conotoxins. Conus venoms have thus received much attention as they could represent a potential source of selective ligands of nAChR subtypes. We describe the mass spectrometric based approaches which led to the discovery of a novel α-conotoxin targeting muscular nAChR from the venom of Conus ermineus. The presence of several posttranslational modifications complicated the N-terminal sequencing. To discriminate between the different possible sequences, analogs with variable N-terminus were synthesized and fragmented by MS/MS. Understanding the fragmentation pathways in the low m/z range appeared crucial to determine the right sequence. The biological activity of this novel α-conotoxin (α-EIIA) that belongs to the unusual α4/4 subfamily was determined by binding experiments. The results revealed not only its selectivity for the muscular nAChR, but also a clear discrimination between the two binding sites described for this receptor. [less ▲]

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See detailPeptide backbone fragmentation initiated by side-chain loss at cysteine residue in matrixassisted laser desorption/ionization in-source decay mass spectrometry
Asakawa, Daiki; Smargiasso, Nicolas ULg; Quinton, Loïc ULg et al

in Journal of Mass Spectrometry [=JMS] (2013), 48

Matrix-assisted laser desorption/ionization in-source decay (MALDI-ISD) is initiated by hydrogen transfer from matrix molecules to the carbonyl oxygen of peptide backbone with subsequent radical-induced ... [more ▼]

Matrix-assisted laser desorption/ionization in-source decay (MALDI-ISD) is initiated by hydrogen transfer from matrix molecules to the carbonyl oxygen of peptide backbone with subsequent radical-induced cleavage leading to c0/z• fragments pair. MALDI-ISD is a very powerful method to obtain long sequence tags from proteins or to do de novo sequencing of peptides. Besides classical fragmentation, MALDI-ISD also shows specific fragments for which the mechanism of formation enlightened the MALDI-ISD process. In this study, the MALDI-ISD mechanism is reviewed, and a specific mechanism is studied in details: the N-terminal side of Cys residue (Xxx-Cys) is described to promote the generation of c0 and w fragments in MALDI-ISD. Our data suggest that for sequences containing Xxx-Cys motifs, the N–Ca bond cleavage occurs following the hydrogen attachment to the thiol group of Cys side-chain. The c•/w fragments pair is formed by side-chain loss of the Cys residue with subsequent radical-induced cleavage at the N–Ca bond located at the left side (N-terminal direction) of the Cys residue. This fragmentation pathway preferentially occurs at free Cys residue and is suppressedwhen the cysteines are involved in disulfide bonds. Hydrogen attachment to alkylated Cys residues using iodoacetamide gives free Cys residue by the loss of •CH2CONH2 radical. The presence of alkylated Cys residue also suppress the formation of c•/w fragments pair via the (Cb)-centered radical, whereas w fragment is still observed as intense signal. In this case, the z• fragment formed by hydrogen attachment of carbonyl oxygen followed side-chain loss at alkylated Cys leads to a w fragment. Hydrogen attachment on peptide backbone and side-chain of Cys residue occurs therefore competitively during MALDI-ISD process. [less ▲]

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See detailTargeting a single function of the multifunctional matrix metalloprotease MT1-MMP. Impact on lymphangiogenesis.
Ingvarsen, Signe; Porse, Astrid; Erpicum, Charlotte ULg et al

in Journal of Biological Chemistry (2013), sous presse

The group of matrix metalloproteases (MMPs) is responsible for multiple processes of extracellular matrix remodeling in the healthy body but also for matrix and tissue destruction during cancer invasion ... [more ▼]

The group of matrix metalloproteases (MMPs) is responsible for multiple processes of extracellular matrix remodeling in the healthy body but also for matrix and tissue destruction during cancer invasion and metastasis. The understanding of the contributions from each individual MMP, both in healthy and pathological events, has been complicated by the lack of specific inhibitors and the fact that some of the potent MMPs are multifunctional enzymes. These factors have also hampered the setup of therapeutic strategies targeting MMP activity. A tempting target is the membrane-associated MT1-MMP which has well-documented importance in matrix degradation but which takes part in more than one pathway in this regard. In this report, we describe the selective targeting of a single function of this enzyme by means of a specific monoclonal antibody against MT1-MMP, raised in an MT1-MMP knock-out mouse. The antibody blocks the enzyme ability to activate proMMP-2 without interfering with the collagenolytic function or the general proteolytic activity of MT1-MMP. Using this antibody, we have shown that the MT1-MMP-catalyzed activation of proMMP- 2 is involved in the outgrowth of cultured lymphatic endothelial cells in a collagen matrix in vitro, as well as in lymphatic vessel sprouting assayed ex vivo. This is the first example of the complete inactivation of a single function of a multifunctional MMP and the use of this strategy to pursue its role. [less ▲]

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See detailIdentity in schizophrenia: A study of trait self-knowledge
Boulanger, Marie ULg; Dethier, Marie ULg; Gendre, Francis et al

in Psychiatry Research (2013)

Identity results from interlock of two systems which are a set of abstracted representations about oneself and a phenomenological self. Literature highlights identity disturbance in schizophrenia that ... [more ▼]

Identity results from interlock of two systems which are a set of abstracted representations about oneself and a phenomenological self. Literature highlights identity disturbance in schizophrenia that affects each of both systems. In the same vein, the present study investigates the stability and the quality of traits self-knowledge, a component of abstracted representations of self, in schizophrenia patients. Sixty-eight patients with schizophrenia and 68 healthy control subjects completed a short version of a personality scale (LABEL). This scale is composed of two versions (A and B), each comprising 50 adjectives that correspond to synonymous adjectives in the alternate list. Participants indicated how these adjectives described themselves and completed both versions of the scale on two separate occasions, one month apart. The findings showed that schizophrenic patients presented an unstable identity and change in identity quality compared with healthy subjects. However, this identity disturbance was weaker than expected. These results are discussed in a part of autobiographical memory disturbances in schizophrenia, illness duration and the decompensation stage. [less ▲]

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See detailIs the bowshock of the runaway massive star HD 195592 a Fermi source?
del Valle, M.; Romero, G.E.; De Becker, Michaël ULg

in Astronomy and Astrophysics (2013), 550

HD 195592 is an O-type super-giant star, known as a well-established runaway. Recently, a Fermi gamma-ray source (2FGL J2030.7+4417) with a position compatible with that of HD 195592 has been reported ... [more ▼]

HD 195592 is an O-type super-giant star, known as a well-established runaway. Recently, a Fermi gamma-ray source (2FGL J2030.7+4417) with a position compatible with that of HD 195592 has been reported. Our goal is to explore the scenario where HD 195592 is the counterpart of the Fermi gamma-ray source modeling the non-thermal emission produced in the bowshock of the runaway star. We calculate the spectral energy distribution of the radiation produced in the bowshock of HD 195592 and we compare it with Fermi observations of 2FGL J2030.7+4417. We present relativistic particle losses and the resulting radiation of the bowshock of HD 195592 and show that the latter is compatible with the detected gamma-ray emission. We conclude that the Fermi source 2FGL J2030.7+4417 might be produced, under some energetic assumptions, by inverse Compton up-scattering of photons from the heated dust in the bowshock of the runaway star. HD 195592 might therefore be the very first object detected belonging to the category of gamma-ray emitting runaway massive stars, whose existence has been recently predicted. [less ▲]

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See detailBolaamphiphiles Derived from Alkenyl L-rhamnosides and Alkenyl D-xylosides: Importance of the Hydrophilic Head
Gatard, Sylvain; Nasir, Mehmet Nail ULg; Deleu, Magali ULg et al

in Molecules (2013), ACCEPTED

The two step synthesis of a new bolaamphiphile derived from alkenyl L-rhamnosides was described. The general synthetic strategy of bolaamphiphiles derived from L-rhamnose was based on a previous work ... [more ▼]

The two step synthesis of a new bolaamphiphile derived from alkenyl L-rhamnosides was described. The general synthetic strategy of bolaamphiphiles derived from L-rhamnose was based on a previous work describing the synthesis of bolaamphiphiles derived from D-xylose. The conformational properties of this new compound were investigated by FTIR spectroscopy in an aqueous film in order to obtain a reference for further studies about the membrane-interacting properties. Moreover, the surface activity of this new bolaamphiphile was analyzed by Langmuir balance technology and was compared with that of the analogous bolaamphiphile derived from alkenyl D-xylosides. The findings indicate that the rhamnoside-based bolaform has an increased surface activity and a better ability to form aggregates than xyloside-based one. [less ▲]

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See detailWorkshops as tools for creative collaboration: finding a balance between facilitation and auto-organization
Elsen, Catherine ULg; Cornet, Adeline; Antoine, Mélanie

in Proceedings of Co-Create 2013 (2013)

This paper analyses four settings of so-called collaborative “creative workshops” (their methods, logistics, regulation processes) and provide peepholes on their respective features, with the informed ... [more ▼]

This paper analyses four settings of so-called collaborative “creative workshops” (their methods, logistics, regulation processes) and provide peepholes on their respective features, with the informed goals of defining criteria for comparison, finding shared essence and distinctive characteristics. Key aspects such as time, facilitation and auto-organization shape a “creative continuum” that formalizes how creativity can be stimulated and how participants might collaboratively develop creative behaviors. [less ▲]

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See detailInvolvement of the lipoxygenase pathway in the systemic resistance induced by Plant Growth-Promoting Rhizobacteria in tomato
Mariutto, Martin ULg

Doctoral thesis (2013)

The treatment of plant roots with some Plant Growth Promoting Rhizobacteria (PGPR) results in the enhancement of the immune capacity of the host against phytopathogens. This phenomenon called Induced ... [more ▼]

The treatment of plant roots with some Plant Growth Promoting Rhizobacteria (PGPR) results in the enhancement of the immune capacity of the host against phytopathogens. This phenomenon called Induced Systemic Resistance (ISR) is observed in the whole plant and is effective against a broad spectrum of diseases. Many previous studies showed that this resistance is associated, in some pathosystems, with the stimulation of the key enzyme of the lipoxygenase (LOX) pathway. This defense pathway synthesizes antibiotic and signal compounds called oxylipins from poly unsaturated fatty acids. The main goal of our work was to analyse the involvement of the LOX pathway during the ISR in tomato plants The different steps of the pathway were analyzed at the transcriptional, enzymatic and metabolic levels in leaves of tomato plants elicited with the PGPR Pseudomonas putida BTP1, before and after infection by the fungal pathogen Botrytis cinerea. Our study showed that the bacterial treatment reprogrammed the LOX pathway. Before infection, the activity of the key enzyme of the pathway, the LOX, was higher in P. putida BTP1-treated plants compared to control plants. This stimulation resulted in the accumulation 13-hydroperoxy fatty acids and 13-hydroxyfatty acids conjugated to phospholipids and galactolipids. These products could constitute a pool of antifungal compounds that can be used during the pathogen attack. During the first days after B. cinerea inoculation, the LOX activity, the accumulation of one of its substrate (linolenic acid) and of its products were stimulated in treated plants as compared to control plants. The increase of LOX activity was caused by the induction of the expression level of two Lox genes: TomLoxD and TomLoxF. We observed a decrease of the pool of conjugated oxylipins and a concomitant increase of non conjugated oxylipins, associated with the stimulation of the phospholipase A2 activity. The branches of the pathway which can use the products of the LOX as substrate were differentially regulated resulting in the accumulation of fungitoxic oxylipins against B. cinerea. In order to determine if the induction of the LOX pathway is a general feature of the ISR in tomato, the effect of other PGPR and resistance elicitors produced by PGPR was analysed. Three pure bacterial elicitors sharing common biosurfactant features (the n-alkylated benzylamine derivative (NABD) produced by P. putida BTP1, the surfactin synthesized by many Bacillus strains and rhamnolipids purified from P. aeruginosa) and six Bacillus strains including strong, medium and non producers of surfactin were applied onto tomato roots. The analysis of the key points of the modulation of the LOX pathway showed that the plant molecular response depended on the studied organ (root / leaf), the nature of the elicitor and the amount of elicitor produced by the PGPR. In addition to our study on the involvement of the LOX pathway, we searched other mechanisms potentially induced in ISR-expressing plants. The combination of histological staining, quantification of enzymatic activities and analysis of the genome expression by hybridization on DNA gene chips showed additional defense genes and pathways involved in the resistance in tomato. In conclusion, our work showed that the LOX pathway is one of the defense mechanisms which can be involved in ISR in tomato. The elicitation of the resistance allows the plant to prepare to a future infection, and to respond faster and in a more effective way to a subsequent pathogen invasion. [less ▲]

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See detailLa connexion entre la politique de coopération au développement et la politique étrangère : le cas de la Roumanie dans la région de la mer Noire
Teclu, Codrat Alin ULg

in Wintgens, Sophie; Grandjean, Geoffrey (Eds.) Acteurs émergents. Perspectives pour la gouvernance mondiale (2013)

Suite à son adhésion à l’Union Européenne le 1er janvier 2007, la Roumanie doit se doter d’une politique de coopération au développement afin d’être en conformité avec l’acquis communautaire. Le présent ... [more ▼]

Suite à son adhésion à l’Union Européenne le 1er janvier 2007, la Roumanie doit se doter d’une politique de coopération au développement afin d’être en conformité avec l’acquis communautaire. Le présent chapitre analyse l’Aide Publique au Développement (APD) roumaine afin de mettre en lumière la manière dont l’État incorpore (ou non) cette dernière dans sa politique étrangère. Nous réalisons une étude de cas sur la mobilisation de l’aide au développement par l’Etat dans sa stratégie d’affirmation comme acteur clé dans la région de la mer Noire, pour la période 2007-2012. Selon l’auteur, l’APD est marginale au sein de la politique étrangère roumaine. Ce manque d’intérêt politique est explicable par la crise économique qui a frappé la Roumanie, un pays qui se relevait seulement des conséquences de la « transition ». Néanmoins, d’autres éléments rentrent également en jeu. Au vu des enjeux géopolitiques de la région, le Ministère des Affaires Étrangères (MAE) conçoit les relations avec les pays riverains de la mer Noire d’abord sous l’angle sécuritaire. La perspective du développement (au sens de lutte contre la pauvreté) est minoritaire. Quatre facteurs institutionnels liés au MAE sont également problématiques : une instabilité politique qui a conduit neuf Ministres différents à la tête du MAE depuis 2007 ; la rotation des diplomates qui nuit à la consolidation d’une mémoire institutionnelle ; le nombre plus réduit d’occasions de socialisation avec le monde du développement par rapport aux ONG ; et enfin la faible attractivité du secteur pour une carrière diplomatique. Pour sa démonstration, l’auteur centre son attention sur la relation entre le MAE et la Fédération des ONG roumaines de Développement (FOND) autour de la région de la mer Noire, en se basant sur une participation observante de sept mois au sein de FOND couplée à une étude monographique des quatre premières éditions (2008 – 2011) du Forum des ONG de la mer Noire ainsi que de documents roumains et européens liés à notre thématique. [less ▲]

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See detailThe Immunomodulating Peptide Thymosin Alpha 1 Has no Effect on Multiple Myeloma Evolution and on Immune Reconstitution
Binsfeld, Marilène ULg; Otjacques, Eléonore ULg; HANNON, Muriel ULg et al

in Belgian Journal of Hematology (2013), Abstracts book(Supplement of 28th General Meeting of the Belgian Hematological Society), 41

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