Last 7 days
Bookmark and Share    
Full Text
See detailNavigating the uncertain seas of controversies – A pedagogical wind rose
Verpoorten, Dominique ULg

in Melard, François; Stassart, Pierre M (Eds.) Proceedings of the International Interdisciplinary Working Conference "Teaching complexity and uncertainty on Environmental Issues - Practices, Theories and Products" (2014, December 23)

The Arlon conference brought together lecturers willing to share and discuss aspects of their practice of “controversies”, seen as a teaching/learning method suited to the contextual needs and demands of ... [more ▼]

The Arlon conference brought together lecturers willing to share and discuss aspects of their practice of “controversies”, seen as a teaching/learning method suited to the contextual needs and demands of higher education environmental studies. The paper contributes to this joint reflective effort by providing conceptual tools helping to interpret the practice, to question what it puts at stake and to detect, from a pedagogical viewpoint, key issues and upcoming questions. The contribution falls within the Conference's aim n°2: “share theories or conceptual propositions that make it possible to equip the teaching approaches and to give them theoretical and methodological foundations” (Program Arlon Conference 2014, p. 2). The article takes a traditional stance, looking at the contributions to the conference through “constructive alignment”(Biggs, 1996), a basic principle meant to secure the pedagogical validity of any given learning situation by establishing a triple consistency (Kovertaite & Leclercq, 2006; Leclercq, 1995; Petit, Castaigne, & Verpoorten, 2007; Tyler, 1949) between objectives, methods and assessment. [less ▲]

Detailed reference viewed: 41 (3 ULg)
Full Text
See detailToward a Consistent Application of International Financial Reporting Standards (IFRSs) across Companies and Countries
Yammine, Mira ULg

Doctoral thesis (2014)

International Financial Reporting Standards (IFRSs) were developed to be a single set of high-quality globally accepted accounting standards. They are meant to allow uniformity in financial reporting ... [more ▼]

International Financial Reporting Standards (IFRSs) were developed to be a single set of high-quality globally accepted accounting standards. They are meant to allow uniformity in financial reporting, enhance comparability of financial statements in the midst of economical globalization, and allow companies with international subsidiaries to prepare their financial statements using similar global standards. The objective of the dissertation is to study whether the application of IFRSs is consistent across companies and countries; in particular we tackle the following two questions: -Does the application of IFRSs in the preparation of financial statements lead to consistency? -Does the public enforcement of accounting standards impact the consistent application of IFRSs? To answer the first question we have addressed standard IAS 36 that was developed by the International Accounting Standards Board (IASB) and covering impairment of assets. We found that managers’ opportunism still has an impact on the impairment of assets recognition and magnitude when taking impairment decisions. To address the second question, a survey covering enforcement of accounting standards activities was prepared and sent to public enforcement bodies in a number of countries mandating the adoption of IFRSs. The survey allowed us to construct an index that represents the activities of the public enforcement bodies. We studied the impact of public enforcement on specific applications of the standards, the results show a decrease in earnings management in countries with effective public enforcement. [less ▲]

Detailed reference viewed: 32 (8 ULg)
Full Text
See detailUn guide de la rédaction du mémoire : pourquoi et comment ?
Grandjean, Geoffrey ULg

Scientific conference (2014, December 19)

Detailed reference viewed: 14 (0 ULg)
Full Text
See detailGathering and Handling of Granular Materials under Microgravity Conditions
Opsomer, Eric ULg

Doctoral thesis (2014)

When a granular material is driven in microgravity environment, one can assist to the formation of dense and slow regions in the system. Indeed, given the dissipative character of the collisions in the ... [more ▼]

When a granular material is driven in microgravity environment, one can assist to the formation of dense and slow regions in the system. Indeed, given the dissipative character of the collisions in the media, energy is lost at each particle interaction and the grains begin to clump locally. The phenomenon has been observed for the first time in the late nineties during sounding rocket experimentation by Falcon and his coworkers and has attracted the interest of many scientists since then. However, precise laws describing the formation and the dynamics of such clusters are still lacking. In order to allow an intensive study of the phenomenon, the European Space Agency set up the SpaceGrains project. Small bronze spheres are enclosed in a rectangular cell and vertically driven by to pistons oscillating in phase opposition. Our work consists in the preparation of the SpaceGrains experiment via molecular dynamics simulations and the elaboration of models predicting the behaviour of the system. Before we started our study concerning SpaceGrains, we reproduced and extended Falcon’s sounding rocket experiments. We showed that, in addition to the granular gas and the cluster, another dynamical regime can be observed in the system. Indeed, for higher filling fractions, the entire granular media behaves like one single completely dissipative particle called the bouncing aggregate. Bouncing modes are observed and can be explained considering the bouncing ball paradigm. Moreover, we highlighted the role of the packing fraction φ as well as the size of the particles R on the different observed dynamics. Within the frame of the SpaceGrains device, we studied the impact of all tunable parameters of the experiment on the dynamics of the system. Thanks to an appropriate scaling all transition points that we obtained by varying the driving amplitude A, the packing fraction and the dimen- sions of the cell L fall along a same theoretical curve. The latter is explained regarding the energy transfer from the piston towards the center of the cell. Once the clustering was controlled, we investigated the handling of the agglomerate. By compartmentalizing the container, local trapping can be achieved and a granular pendant of Maxwell’s demon can be observed in microgravity. Based on the measured particle flux between the compartments, we realized a theoretical model predicting the asymptotic steady state of the system depending on the total number N of particles. In a clustered system, we investigated the impact of asymmetrical driving on the system’s dy- namics. We showed that the mean position of the cluster can be fully controlled via the amplitude ratio a. Moreover, the natural fluctuations of the agglomerate around its equilibrium position are dictated by the driving frequency f and the mass of the cluster. Finally, we realized simulations of driven bi-disperse gases and investigated the segregation phenomena in the system. We showed that clustering and segregation are strongly linked and that the size and the mass of the particles impact the segregation dynamics in different ways. [less ▲]

Detailed reference viewed: 25 (5 ULg)
See detailDécouverte d'un gène du gigantisme
Beckers, Albert ULg

Scientific conference (2014, December 19)

Detailed reference viewed: 7 (0 ULg)
See detailUn exemple de détournement du jeu vidéo par les joueurs : la pratique du speedrun
Barnabé, Fanny ULg

Scientific conference (2014, December 18)

Detailed reference viewed: 17 (0 ULg)
See detailHors-cadre : quand le documentaire ne peut pas tout montrer
Hamers, Jérémy ULg

Scientific conference (2014, December 18)

Detailed reference viewed: 20 (2 ULg)
Full Text
See detailEtude et Modélisation de la contribution des macrophytes flottants (Lemna minor) dans le fonctionnement des lagunes naturellles
Tangou Tabou, Thierry ULg

Doctoral thesis (2014)

Waste stabilization ponds (WSP) are widely used in the treatment of domestic and industrial wastewaters in industrialized countries and in developing countries including the Democratic Republic of Congo ... [more ▼]

Waste stabilization ponds (WSP) are widely used in the treatment of domestic and industrial wastewaters in industrialized countries and in developing countries including the Democratic Republic of Congo. Several organisms (e.g., bacteria, algae, protozoa, and aquatic plants) are involved in the treatment process occurring in the WSP. Therefore, the performance of such systems strongly depends upon the management of the existing macrophytes (or at least the management of the most dominant species). Indeed, large biomass coverage of floating macrophytes can induce several dysfunctions within the ponds (e.g., second pollution due to the death and the settling of the macrophytes, difficulties in harvesting valuable biomass for animal feeding or pharmaceutical purposes, etc.) and can threaten the surrounding ecosystems (i.e., fauna and flora) through the releases of partially or unpurified waters. This study aimed at investigating the contribution of floating macrophytes, namely that of duckweed (Lemna minor), which are the most common species, in the functioning of natural WSP. Specifically, the objectives were: (i) the modeling of the growth kinetics of Lemna minor based on the key environmental influencing factors (nutrients concentrations, light intensity, pH, temperature); (ii) the characterizations of the stoichiometry of the growth of Lemna minor and the gas/liquid transfers in the ponds; and (iii) the set-up of a mathematical model, i.e. the MLLE (Modèle de Lagunage par Lentilles d'eau) through the mass balances approach based on the Petersen’s matrix. The study of the growth kinetics of L. minor was carried out in a growth chamber (phytotron) using a pilot involving initial fresh L. minor biomass and different set of concentrations in nutrient nitrogen (N) and phosphorus (P), light intensity, pH, and temperature. The growth was monitored using the gravimetric methods and digital images processing. The results showed that the growth of L. Minor was optimum for concentrations of N and P of circa 10 mg N-NH4+.L-1 and 1 mg P-PO43-.L-1, respectively, with a maximum growth rate of approximately 0.09 j-1. However, the growth decreased progressively when the respective concentrations of N and P reached approximately 16 mg N-NH4+L-1 and 6 mg P-PO43-.L-1. A possible inhibition due to the excess of substrates might be the cause of such decrease. Moreover, a light intensity greater than 450 μmol.m-².s-1 was potentially harmful for L. minor surviving (growth rate dropped by 60%), though the mortality rate was low (< 0.05 j-1). Characterizing the stoichiometry of L. minor resulted in the formulation of the plant biomol (C88H163O70N10P) and the description of biochemical processes occurring within the ponds, as well as the determination of the conversion rates substrate-biomass in each of these processes. The Lavoisier's principle (conservation of the matter) also was confirmed for the stoichiometric relationship developed for chemical oxygen demands through our experiments. Furthermore, the aeration test assays revealed the influence of the biomass coverage on the gaz/liquid transfers parameters. An exponential decrease of the transfer coefficients, the oxygenation capacity and hourly inputs was associated with increasing coverage rates in both absence and presence of plants. The hypothesis of a main release (about 80%) of oxygen (consumption of carbon dioxide) toward the atmosphere was also confirmed. The third fold of our study was then undertaken based on our findings on the biochemical processes (kinetics and stoichiometry of growth and mortality of plants) and findings on physical processes (gas/liquid transfer) occurring in WSP. A mathematical model, the MLLE, was thus developed and its validity assessed through the equilibrium of the Petersen's matrix. The impact of key nutrients (i.e., nitrogen and phosphorus) on the growth of duckweed, along with the influence of the release of oxygen (consumption carbon dioxide) by the floating macrophytes (L. minor) can be, therefore, thoroughly assessed based on the state variables of the model. Additional simulations using the West® (World Wide Engine for Simulation, Training and Automation) software could help in further calibrating and/or validating the MLLE, and guide in its implementation for industrial purposes. Keywords: Lemna minor, biomass, image processing, nutrient, biomole, gas/liquid transfer, model [less ▲]

Detailed reference viewed: 31 (8 ULg)
See detailEtude de step-down des corticostéroïdes inhalés chez des patients asthmatiques non-éosinophiliques
Demarche, Sophie ULg

Scientific conference (2014, December 18)

Detailed reference viewed: 20 (1 ULg)
Full Text
See detailContribution to the epidemiology of bovine brucellosis in Ivory Coast
Sanogo, Moussa ULg

Doctoral thesis (2014)

Bovine brucellosis is an endemic infectious disease which can negatively impact on cattle productivity and welfare as well as on human health. In many developing countries such as Ivory Coast, there is a ... [more ▼]

Bovine brucellosis is an endemic infectious disease which can negatively impact on cattle productivity and welfare as well as on human health. In many developing countries such as Ivory Coast, there is a need for knowledge on the distribution and the frequency of the disease (or evidence of its presence) within the animal population and the possible factors associated with the disease. Information is also needed on species and biovars of Brucella at national and regional scales, on the performance of commonly used diagnostic tests for accurate estimation of the true disease prevalence, and on determination of risk factors associated with the disease. These informations are of key importance to set up and implement appropriate and efficient prevention and control measures against brucellosis. For these reasons, the research presented in this thesis aimed to contribute to a better understanding of the epidemiology of bovine brucellosis in Ivory Coast. The thesis is structured into three main parts. The introduction part includes three chapters. The first chapter presents an overview of the literature on the pathogen causing brucellosis, their characteristics and distribution. The impact and the existing strategies for preventing and controlling brucellosis are discussed with a particular reference to the situation of bovine brucellosis in Ivory Coast. The presence and the importance of the disease were confirmed in the country but the disease is still uncontrolled. In the second chapter (Chapter 2), an insight on statistical, epidemiological principles and concepts applied to achieve the different objectives (Chapter 3) is given, including a discussion on available approaches to estimate diagnostic test characteristics and the true prevalence of a disease. The second part of the thesis includes research on different aspects of the epidemiology of bovine brucellosis in Ivory Coast and West Africa (Chapter 4, 5, 6 and 7). Chapter 4 specifically provides a state-of–the-art knowledge on species and biovars of Brucella reported in cattle from Ivory Coast and all other countries of West Africa, through a review of available literature. From the synthesized literature, Brucella abortus was demonstrated to be the most prevalent species in cattle in West Africa, in line with the known host preference for Brucellae. So far, biovars 3 appeared to be commonly the most isolated in West Africa and was also recently identified in Ivory Coast. However, the presence of B. melitensis and/or B. suis was not reported yet in cattle in this part of Africa. Results on prevailing strains of Brucella in cattle were related with commonly used serological diagnostic tools. Thus, chapter 5 was dedicated to verify their appropriateness and to assess the performance of two serological tests, Rose Bengal Test (RBT) and indirect Enzyme-linked Immunosorbent assay (iELISA). Using a Bayesian approach, the correlation-adjusted sensitivity of iELISA was estimated at 96.1 % (Credibility Interval (CrI): 92.7-99.8) whereas that of RBT was 54.9 % (CrI: 23.5-95.1). High correlation-adjusted specificities were found for both tests, 95.0 % (CrI: 91.1-99.6) for iELISA and 97.7 % (CrI: 95.3-99.4) for RBT, respectively. The true prevalence of brucellosis was also estimated using the 1228 tested serum samples to be 4.6 with a 95% credibility interval ranging from 0.6 to 9.5% (Chapter 5 and 6). These results also revealed a good performance for the iELISA, which might consequently be a valuable screening assay under the epidemiological conditions prevailing in Ivory Coast. In Chapter 7, risk factors associated with bovine brucellosis seropositivity were investigated using serological results obtained from 907 serum samples collected from unvaccinated cattle of at least 6 months of age in the savannah-forest region of Ivory Coast. Serum samples were tested using the Rose Bengal test (RBT) and indirect enzyme linked immunosorbent assay (iELISA). The logistic regression analysis indicated that brucellosis seropositivity was associated with age and herd size. Cattle above 5 years of age were found to be more likely seropositive (Odd Ratio (OR) =2.8; 95% Confidence Interval (CI): 1.3, 6.4) compared to cattle under 3 years of age. Similarly, the odds of brucellosis seropositivity for herds with more than 100 cattle was 3.3 (95% CI: 1.2, 8.9) times higher compared to those with less than 50 cattle. The third part presents a general discussion on the overall contribution of the current research (Chapter 8), by highlighting the main results and pointing out their significance. The need for more investigations on the epidemiology of brucellosis, in Ivory Coast and at West African scale, is highlighted. It is neccessary to provide additional knowledge on prevailing field strains of Brucella, on the distribution of the disease and on associated risk factors to implement preventive and control measures. Finally, for more cost-effectiveness and efficiency, the need to strengthen the capabilities of the veterinary services and national laboratories and to consider the control of brucellosis and other zoonotic diseases through a regional, integrated and collaborative perspective is also highlighted. [less ▲]

Detailed reference viewed: 14 (3 ULg)
Full Text
Peer Reviewed
See detailCyclovirus CyCV-VN species distribution is not limited to Vietnam and extends to Africa
Garigliany, Mutien-Marie ULg; Hagen, Ralf Mathias; Frickmann, Hagen et al

in Scientific Reports (2014), 4

Cycloviruses, small ssDNA viruses of the Circoviridae family, have been identified in the cerebrospinal fluid from symptomatic human patients. One of these species, cyclovirus-Vietnam (CyCV-VN), was shown ... [more ▼]

Cycloviruses, small ssDNA viruses of the Circoviridae family, have been identified in the cerebrospinal fluid from symptomatic human patients. One of these species, cyclovirus-Vietnam (CyCV-VN), was shown to be restricted to central and southern Vietnam. Here we report the detection of CyCV-VN species in stool samples from pigs and humans from Africa, far beyond their supposed limited geographic distribution. [less ▲]

Detailed reference viewed: 19 (3 ULg)
Peer Reviewed
See detailUncertainty in Training-Image Based Inversion of Hydraulic Head Data Constrained to ERT Data: Workflow and Case Study
Hermans, Thomas ULg; Nguyen, Frédéric ULg; Caers, Jef

Conference (2014, December 18)

In inverse problems, investigating the relationship between data and prior models and the uncertainty related to the posterior distribution of model parameters are as important as matching the data. In ... [more ▼]

In inverse problems, investigating the relationship between data and prior models and the uncertainty related to the posterior distribution of model parameters are as important as matching the data. In recent years, many efforts have been done to assess the posterior distribution of a given problem with reasonable computational costs through inversion techniques such as McMC. The derived posterior distribution is always dependent on the prior distribution. However, most of the studies ignore modeling the prior with realistic uncertainty. In this paper, we propose a workflow to assess the uncertainty of inversion of hydraulic heads data through the addition of electrical resistivity tomography (ERT) constraining data. The workflow is divided in three successive steps: 1) Construction of prior: we generate multiple alternative geological scenarios from literature data (architecture of facies) as well as site specific data (proportions of facies). Spatial uncertainty within each scenario is integrated hierarchically through geostatistics (multiple-point statistics simulation of facies constrained by ERT data as soft data). 2) Validation of prior scenarios: we transform prior facies scenarios into resistivity distribution scenarios through forward and inverse modeling. The scenarios are validated by comparison with field ERT data. The comparison is made through distance calculation and projection into a low dimensional space to calculate the probability of each scenario given field ERT data. 3) Matching dynamical data: we use the probability perturbation method, within each scenario, to integrate hydraulic heads to our models. We account for scenario probabilities, calculated in 2, in determining how many models per scenario we have to consider for building a reliable posterior distribution. As an illustration, the method is applied on a field case study in an alluvial aquifer (Belgium) where we consider prior uncertainty related to the type of elements (gravel channels or bars) and to their size. This study shows the importance of considering the uncertainty of the prior in inverse problems as it has a strong influence on model predictions and decision-making problems. [less ▲]

Detailed reference viewed: 30 (1 ULg)
Full Text
See detailEtude de l'assainissement non collectif en Afrique Subsaharienne : Application à la ville de Bujumbura
Bigumandondera, Patrice ULg

Doctoral thesis (2014)

Summary On-site sanitation sector in sub-Saharan African is a complex area that requires the involvement of a multidimensional approach mobilizing various actors and competencies. The main purpose of this ... [more ▼]

Summary On-site sanitation sector in sub-Saharan African is a complex area that requires the involvement of a multidimensional approach mobilizing various actors and competencies. The main purpose of this thesis is to conduct a study integrating the three links in the sanitation chain that compose the on-site sanitation sector, namely the upstream links regarding sanitation facilities installed at private homes, the intermediate link for evacuation of sludge and transport to a treatment site and the downstream link for disposal and treatment sludge. A sociological approach mobilizing both survey techniques and on-site observation has allowed understanding the on-site sanitation practices in the field of developing countries. Methodologies to initiate corrective action with respect to what is done so far are utilized. Such as a method for evaluating on-site sanitation using an indicator that is a combination of indicators developed taking into account the entire sanitation sector. At the same time, a procedure to select sanitation technologies based on a number of criteria was adopted. The proposed technologies as well as criteria used for their implementation follow the same logic as previously, i.e. that consisting of taking into account the entire sanitation sector. This study also examined the design procedure of the septic tank and of evaluated its performance on site given that it is used in many sanitation systems. Settling column tests were carried out show that the effluent who enters in the septic tank settles well but settable solids remain in the effluent leaving the septic tank remaining, parameter that needs to be taken into account in the establishment of the infiltration bed after the septic tank. Quantification of sludge accumulation rates in the septic tank shows that for a pump out period every 4 years, the design can be based on an average sludge accumulation rate of 0.22L/person/day. These values (as expressed in g COD/person/day) are useful both to assess withdrawal, but also to design sludge's disposal site. Analyses of these sludges reveal a poorly biodegradable product with a non-biodegradable COD fraction reaching 47.5%, thus as a product with a low methanogenic potential, limiting the interest of its application in anaerobic digestion as a substrate. But, their co-treatment with organic solids waste in a partial anaerobic co-digestion process followed by composting. In this case sludges are considered as digestion inoculums. Preliminary results are encouraging, particularly in terms of total time needed for the entire process. Among other advantages, this process reduces handling of the waste being processed and provides two end products directly recoverable: compost and methane. So we hope that this research will contribute to the establishment in African countries for more efficient on-site sanitation, able to meet the targets set by international organizations. [less ▲]

Detailed reference viewed: 33 (5 ULg)
See detailLa charge vocale et son incidence sur le traitement orthophonique
Remacle, Angélique ULg

Scientific conference (2014, December 17)

Les troubles de la voix peuvent être engendrés tant par un malmenage que par un surmenage vocal. Tandis que le malmenage est causé par un geste vocal inadéquat, le surmenage résulte d’une charge vocale ... [more ▼]

Les troubles de la voix peuvent être engendrés tant par un malmenage que par un surmenage vocal. Tandis que le malmenage est causé par un geste vocal inadéquat, le surmenage résulte d’une charge vocale supérieure aux capacités de récupération de l’individu. Dans cet atelier, nous expliquerons ce qu’est la charge vocale, nous la replacerons dans le contexte des stress mécaniques appliqués aux plis vocaux lors de la phonation et nous exposerons les conséquences d’une surcharge vocale. Nous présenterons ensuite les systèmes portables de monitoring de la voix commercialisés depuis quelques années : les dosimètres ou accumulateurs de voix. Ces outils permettent d’objectiver le comportement vocal en situation réelle pendant une durée prolongée et aident à cibler les situations favorisant la dysphonie. Enfin, nous discuterons des apports du feedback vibratoire dans le but de modifier le comportement vocal en temps réel. [less ▲]

Detailed reference viewed: 9 (1 ULg)
Full Text
See detailGenetic improvement of pig sire lines for production performances in crossbreeding
Dufrasne, Marie ULg

Doctoral thesis (2014)

Crossbreeding is widely used in pig breeding to benefit from advantages of heterosis effects and breed complementarity. Breeding programs are specialized between sire and dam lines, with different ... [more ▼]

Crossbreeding is widely used in pig breeding to benefit from advantages of heterosis effects and breed complementarity. Breeding programs are specialized between sire and dam lines, with different selection objectives. Sire lines are mainly selected for production traits (e.g., growth, carcass quality, feed efficiency). Moreover, selection is often in purebred lines while the goal of selection is to improve crossbred performances. Hence, genetic selection of purebred parents involved in crossbreeding requires models accounting for crossbreeding effects, and able to combine data from different genetic types (purebreds and crossbreds) and different environmental conditions. Therefore, the objective of this thesis was to develop genetic models to estimate genetic parameters and breeding values for production traits in crossbred populations of pigs. A genetic model was developed to estimate the genetic potential of Walloon purebred Piétrain boars for growth performances in crossbreeding based on test station and on-farm data from purebred and crossbred pigs. Weight records from both systems were considered as different traits. Results showed that weights recorded in test station and on-farm were different traits but genetically correlated. Moreover, combining both sources of data allowed to increase reliability of estimated breeding values and Genotype x Environment interactions were detected for growth in the Walloon breeding program. The introduction of dominance effects, linked to crossbreeding, into the genetic model for growth in the crossbred population showed that dominance effects existed on growth of crossbred pigs. Moreover, the total and the additive genetic merits were better estimated than with a strictly additive model. Research conducted on commercial crossbred data from US Duroc sires displayed that the sire genetic effects were not negligible on traits like piglet birth weight, preweaning mortality, mortality at different stages of the grow-finishing period and hot carcass weight. Furthermore, sire genetic effects increased with age. Genetic studies of mortality in the population from US Duroc sires showed that mortality traits had a low heritability, increasing with age and that mortality was not antagonistic with market weight. Besides, genetic parameters estimated for birth weight and its relation with other production traits in crossbred populations from Piétrain and Duroc sires showed that birth weight could be used as an early indicator trait to improve latter performances. Finally, production traits were genetically correlated and should be selected simultaneously to reach the breeding goal. [less ▲]

Detailed reference viewed: 39 (13 ULg)
Full Text
See detailImpact of abiotic and biotic factors on VOC emissions and protein expression in Arabidopsis thaliana (L.) Heynh
Hien, Truong Thi Dieu ULg

Doctoral thesis (2014)

The interaction of Arabidopsis and environmental factors is a model system used to study stress response in plants, in particular the analysis of the emission of volatile organic compounds (VOCs) and the ... [more ▼]

The interaction of Arabidopsis and environmental factors is a model system used to study stress response in plants, in particular the analysis of the emission of volatile organic compounds (VOCs) and the proteomic expression. In the present thesis, VOC emissions and expression of proteins were studied in the response of Arabidopsis thaliana (L.) Heynh to the interaction of abiotic (temperature or water stress) and biotic (adults Myzus persicae (Sulzer) or Plutella xylostella (L.) larvae) factors. To achieve these objectives, the volatile profiles emission of Arabidopsis was previously evaluated by headspace solid phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). The experimental conditions were maintained for different periods, and the emission profile for each period was determined. Our main findings were as follows: (1) the combined aphids (M. persicae) and temperature stress treatments induced more complex plant volatile profiles than did single stress. Rising in temperatures (17, 22, and 27 °C) led to significant increases of isothiocyanate (ITC), nitrile, and sulfide proportions in aphid-infested Arabidopsis plants; (2) aphid-infested water-stressed Arabidopsis exhibited significant changes in their VOC emission blends with modification of sulfide, ITC, ketones, aldehyde, and terpenes; and (3) the molecule profiles from P. xylostella-infested plants also varied with infestation time and temperature treatment. The larvae-infested Arabidopsis at 22 °C emitted sulfides and nitrile instead of ITCs as it is the case at 17 and 27 °C. Additionally, a proteomic approach using two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry was performed to examine the protein changes in Arabidopsis responses to herbivorous insects under controlled conditions. A total of 574 and 454 protein spots were reproducibly detected by bidimensional electrophoresis. After M. persicae and P. xylostella infestations 31 and 18 protein spots were differently expressed in their relative abundance, respectively. Sixteen proteins were successfully identified by MALDI-TOF MS and LC-ESI-MS/MS. Functional analysis of identified proteins showed that they were associated with a large number of biological processes, namely carbohydrate, amino acid, lipid and energy metabolism, photosynthesis, defense and translation process. The expression of such proteins in A. thaliana leaves was either up-regulated or down-regulated according to insect feeding stresses. Taken together, the original reported results provide evidences that the interaction between abiotic and biotic stress factors has great ability to alter the profile of VOCs as well as proteins in A. thaliana plants. It provides valuable new insights to explore the complex response of plants to multiple simultaneous factors. [less ▲]

Detailed reference viewed: 24 (7 ULg)
Full Text
See detailAllergie moléculaire & Diagnostic in-vitro, Optimalisation de la prescription
GADISSEUR, Romy ULg

Conference (2014, December 17)

Detailed reference viewed: 8 (2 ULg)
Full Text
See detailComment alléger la charge des ménages dans le financement de la santé dans les pays du Sud: Résultats des Comptes Nationaux de la Santé 2010-11 en RD Congo
Manzambi Kuwekita, Joseph ULg

Learning material (2014)

Cette étude examine la part et l’affectation des dépenses des ménages dans le financement de la santé telle que présentée dans le rapport des Comptes Nationaux de la Santé 2010-11. Les dépenses courantes ... [more ▼]

Cette étude examine la part et l’affectation des dépenses des ménages dans le financement de la santé telle que présentée dans le rapport des Comptes Nationaux de la Santé 2010-11. Les dépenses courantes de santé sont à charge des ménages (40%) et réalisées par paiement direct sans partage de coûts (93,7%). L’hospitalisation est justifiée pour soigner les carences nutritionnelles (34%), le paludisme (12%) et les troubles mentaux (10%). Dans 82% des cas, les soins ambulatoires concernent le paludisme (71%) et les troubles mentaux (12,5%). Le fait que:71,58% des dépenses des hôpitaux, 96,49% des dépenses des centres ambulatoires, 98,48% des dépenses des labo, 72,42% des dépenses des pharmacies sont à charge des ménages: montre la nécessité de la mise en place de mécanismes de solidarisation du risque santé. le paludisme absorbe 71% des dépenses en soins ambulatoires: d’où la nécessité de renforcer les mesures de prévention, le traitement et l’assainissement du milieu. L’amélioration du pouvoir d’achat permettrait d’autonomiser les ménages dans la lutte contre le paludisme. les carences nutritionnelles sont la première cause de recours à l’hôpital: d’où la nécessité de changement des comportements et habitudes alimentaires, la sécurité alimentaires et l’amélioration du pouvoir d’achat des ménages. les troubles mentaux sont le troisième motif d’hospitalisation: d’où la nécessité de les prendre en compte dans les mécanismes de financement de la lutte contre la maladie. [less ▲]

Detailed reference viewed: 17 (2 ULg)
Full Text
See detailBiostratigraphic and chemostratigraphic constraints of the Mbuji-Mayi Supergroup, Democratic Republic of Congo
Kabamba Baludikay, Blaise ULg; Bekker, Andrey; Baudet, Daniel et al

Poster (2014, December 16)

Detailed reference viewed: 16 (0 ULg)