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See detailModel polyQ proteins based on the beta-lactamase BlaP: How non-polyQ regions influence the polyQ length-dependent aggregation process
Huynen, Céline ULg

Doctoral thesis (2015)

Neurodegenerative amyloid diseases are fatal disorders representing an important human health and economic burden. Amongst them, nine disorders are classified as polyglutamine (polyQ) diseases, for which ... [more ▼]

Neurodegenerative amyloid diseases are fatal disorders representing an important human health and economic burden. Amongst them, nine disorders are classified as polyglutamine (polyQ) diseases, for which no treatment is yet available. They are all characterized by the pathological expansion of a poly(CAG) sequence, above a specific threshold, within the coding sequence of nine unrelated genes, translated in nine polyQ proteins. The polyQ expansion, the only common point, is the critical determinant for polyQ disease development by triggering protein aggregation into amyloid fibrils. More recent studies however indicate that the protein context modulates the polyQ-induced aggregation process and the disease phenotype. The objectives of my thesis are to deeper investigate the molecular determinants of the complex interplay between the propensity of the polyQ tract to trigger protein aggregation and the modulating role of non-polyQ regions in order to allow the identification of strategies to interfere with the pathological aggregation process. For that purpose, model polyQ proteins, referred to as BlaP-polyQ chimeras, based on the β-lactamase BlaP and polyQ sequences (23 – 79Q) inserted at two positions (197, in between or not unstructured peptides, or 216), are used and their aggregation properties are characterized under several conditions. We first observe that the polyQ length is determinant for BlaP-polyQ chimera aggregation. Indeed, there is a Q-threshold for the aggregation into amyloid fibrils and for fibril elongation. Above this threshold, the aggregation in solution and the elongation rate increase with the length of the polyQ tract, with an exponential rise-to-maximum and a linear regression, respectively, independently of the conformation of the BlaP moiety, and of the position of the polyQ tract within BlaP. Longer polyQ tracts are likely to have a larger conformational flexibility allowing them to more easily adopt an amyloid-aggregation prone conformation. However, the Q-threshold for fibril elongation is much lower than for fibril formation in solution: the polyQ tract requires a larger conformational flexibility to nucleate the formation of fibrils than to elongate them. Secondly, the Q-threshold for fibril formation is lower once the BlaP moiety is unfolded, and BlaP-polyQ chimeras aggregate faster into amyloid fibrils under conditions favoring the unfolding of the BlaP moiety. The native structure of BlaP is likely to impose conformational constraints to moderate and long polyQ tracts that block and decrease, respectively, their propensity to form fibrils. The effects of constraints decrease with the polyQ length. Thirdly, BlaP chimeras with the polyQ tract in position 216 have an increased propensity to trigger the nucleation and the elongation of amyloid fibrils compared to chimeras with the polyQ in position 197. Advanced studies confirm that the propensity of the polyQ tract to aggregate into amyloid fibrils is linked to the conformational flexibility of the polyQ tract, which depends on (i) the polyQ length, (ii) the location of the polyQ tract within BlaP, i.e., a terminal location or embedded within a protein domain, and (iii) the structural properties of the polyQ flanking regions. Finally, we observe that the whole region flanking the polyQ tract in position 197 at its N-terminus has an anti-aggregating property that fully counterbalances the pro-aggregating property of that flanking at the C-terminus. The former imposes strong conformational constraints to the polyQ tract that reduce its conformational flexibility and hence its aggregation propensity. Moreover, these regions are likely to differently affect the solubility of the polyQ protein and hence the driving force for insoluble aggregation. Altogether, our data also suggest that BlaP chimeras aggregate via the commonly described nucleation-dependent polymerization mechanism during which the aggregation is first triggered by polyQ-polyQ interactions, and then a subsequent slight reorganization of the BlaP moiety is required for conversion of aggregates into amyloid fibrils. Based on these results, we should allow the development of therapeutic strategies, targeting specifically the molecular features of the complex interplay between the polyQ and non-polyQ regions during the nucleation and/or elongation of the pathological aggregation of polyQ proteins. [less ▲]

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See detailPourquoi se mobiliser pour la planète rouge?
Stiepen, Arnaud ULg

Conference given outside the academic context (2015)

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See detailDes co-enquêteurs coriaces. Quand l'explicitation du travail de terrain complique les choses
Vangeebergen, Thomas ULg

Conference (2015, June 18)

La souplesse et la plasticité des problématiques abordées sont sans doute parmi les attraits majeurs que peuvent présenter les recherches qualitatives. Cette ouverture à une reconfiguration permanente des ... [more ▼]

La souplesse et la plasticité des problématiques abordées sont sans doute parmi les attraits majeurs que peuvent présenter les recherches qualitatives. Cette ouverture à une reconfiguration permanente des questions de recherches réside (entre autres) dans la place que l'on accorde aux acteurs. Plus que des « informateurs », ceux-ci peuvent participer véritablement à l'enquête, et sont alors en fait à la manoeuvre autant que le chercheur. D'acteurs, ils gagnent alors le statut de co-enquêteur. En nous renvoyant nos propres questions, en les reformulant, en les faisant apparaître sous un angle différent, les acteurs contribuent à (re)dessiner nos recherches. [less ▲]

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See detailCompétition partisane, changement climatique et écologie politique : convergences et conflits sur les thématiques politiques
Piet, Grégory ULg

in Flipo, Fabrice (Ed.) Les actes du Colloque "Penser l'écologie politique" (2015, June 16)

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See detailEtude de la sémaphorine virale codée par le gène A3 de l’herpèsvirus alcélaphin 1
Myster, Françoise

Doctoral thesis (2015)

Among gammaherpesvirus, the Macavirus genus is composed of viruses associated to malignant catarrhal fever (MCF) and other phylogenetically related viruses. MCF is a frequently fatal lymphoproliferative ... [more ▼]

Among gammaherpesvirus, the Macavirus genus is composed of viruses associated to malignant catarrhal fever (MCF) and other phylogenetically related viruses. MCF is a frequently fatal lymphoproliferative disease. Three macaviruses inducing MCF have been entirely sequenced: alcelaphine herpesvirus 2 (AlHV-2), ovine herpesvirus 2 (OvHV-2) and alcelaphine herpesvirus 1 (AlHV-1). Sheep carries OvHV-2 asymptomatically while wildebeest is infected with AlHV-1 without developing any clinical signs or lesions. Both viruses represent the most studied macaviruses. In susceptible ruminants, OvHV-2 and AlHV-1 induce the sheep-associated form and the wildebeest-derived form of MCF (WD-MCF), respectively. Economic consequences of WD-MCF are significant in sub-Saharan Africa. WD-MCF is characterized by the proliferation and infiltration of lymphoblastoid T cells surrounding blood vessels and can be considered as a model for peripheral T cell lymphoma caused by a virus. Rabbits are used as experimental model to study MCF. This species develops clinical signs and lesions that they are indistinguishable from those observed in other susceptible species. Until recently, available data on WD-MCF pathogenesis were limited to the simple description clinical signs and lesions. Recently, it was demonstrated that CD8+ T cells proliferate and that this cellular expansion is associated with a severe increase of the viral load in PBMC and lymphoid organs (Dewals et al., 2008). The cloning of the AlHV-1 genome as an infectious and pathogenic bacterial artificial chromosome (BAC) has greatly facilitated the study of individual gene of AlHV-1 (Dewals et al., 2006). Among herpesviruses, viral semaphorins can only be found in members of the Macavirus genus. OvHV 2 encodes Ov3, and AlHV-2 and AlHV-1 encode A3, both genes encoding a semaphorin homolog. Semaphorins are proteins characterized by a conserved amino-terminal domain, the SEMA domain. The roles of the semaphorins on cytoskeleton dynamics have been widely studied. Viral semaphorins could mediate immune evasion mechanisms or viral dissemination and could be involved in specific properties of macaviruses. The first study of the present thesis was dedicated to the investigation of the pathogenesis of WD-MCF and the role of latency. We investigated the distribution of the AlHV-1 infection in the lesions and demonstrated that the infiltration of CD8+ T cells in different lymphoid and non-lymphoid organs and tissues is directly associated with a non-productive viral infection. The second study focused on the A3 gene of AlHV-1 and its potential functions during WD-MCF. We showed that the A3 gene is expressed during the early phase of the viral infection and encodes a functional semaphorin that was termed AlHV-sema. AlHV-sema was able to induce cell retraction. Despite the observed independent acquisition of pox- and herpesvirus semaphorins, AlHV-sema inhibited phagocytosis by dendritic cells and migration to the draining lymph node through mechanisms similar to poxvirus semaphorin. AlHV-sema could also facilitate viral dissemination or confer immune evasion functions. Next, we investigated whether AlHV-sema could affect WD-MCF induction. We did not observe any effect of the absence of AlHV-sema expression during the development of WD-MCF after rabbit nasal infection. Macaviruses are swine and ruminant gammaherpesviruses responsible for a latent asymptomatic infection in their natural species. The development of MCF in other ruminant susceptible species is due to cross-species transmission. During evolution, the gene selection in susceptible species is certainly reduced due to the fact that these species are dead-end hosts. Thus, it is difficult to address the role of AlHV-1 specific genes in MCF as these genes have evolved in other species. Nevertheless, we brought in this work important insight for our understanding of the pathogenesis of WD-MCF and we identified AlHV-sema as a potential immunoevasion factor. DEWALS B., MYSTER F., PALMEIRA L., GILLET L., ACKERMANN M., VANDERPLASSCHEN A. Ex vivo bioluminescence detection of alcelaphine herpesvirus 1 infection during malignant catarrhal fever. J. Virol., 2011, 85, 6941-6954. MYSTER F., PALMEIRA L., SOREL O., BOUILLENNE F., DEPAUW E., SCHWARTZ-CORNIL I., VANDERPLASSCHEN A., DEWALS B.G. Viral semaphorin inhibits dendritic cell phagocytosis and migration but is not essential for gamma-herpesvirus-induced lymphoproliferation in malignant catarrhal fever. J. Virol., 2015. [less ▲]

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See detailSeagrass macrophytodetritus: a copepod hub - Species diversity, dynamics and trophic ecology of the meiofauna community in Posidonia oceanica leaf litter accumulations
Mascart, Thibaud ULg

Doctoral thesis (2015)

Macrophytodetritus is a heterogeneous mixture of detrital material that accumulates on submerged unvegetated sand patches amid vast Mediterranean Posidonia oceanica seagrass meadows. Several vagile ... [more ▼]

Macrophytodetritus is a heterogeneous mixture of detrital material that accumulates on submerged unvegetated sand patches amid vast Mediterranean Posidonia oceanica seagrass meadows. Several vagile invertebrates are present in substantial biomass and biodiversity. Among these invertebrates, meiofauna (fauna between 38µm and 1mm) is ubiquitous and seems to play a key-feature in this dynamic and patchy system. Coastal ecosystems are under the direct effect of anthropogenic disturbance and degradation. Extra research is crucially needed to understand better the dynamics of coastal vegetation, in order to have a more successful restauration of these regressing ecosystems. In this context, the main goal of this PhD research was triple: (1) characterising in situ the physico-chemistry and the composition of the macrophytodetritus accumulations in the Calvi Bay, Corsica, (2) identifying the diversity of the associated meiofauna communities, especially harpacticoid copepods together with unravelling the origin of the present copepods and (3) characterizing the trophic ecology of the copepod communities in the macrophytodetritus at the specific and eco-morphological level. This research showed that macrophytodetritus biomass is composed on average for 75% of dead P. oceanica seagrass leaves shed after senescence. Attached to the surface of the seagrass leaves numerous micro- and macroepiphytes are present, representing on average 10% of the total biomass. The remaining part is mainly constituted of drift material, like detached P. oceanica shoots and epilithic macroalgae. A seasonal pattern is observed regarding the amount of accumulated material and the physico-chemical composition inside the accumulation. Wind-induced hydrodynamics is the responsible driver behind the variability of the macrophytodetritus and consequently it has a major impact on the faunal communities already present in a macrophytodetritus accumulation. Previous studies showed that the presence of macrofaunal invertebrates (> 1 mm) in high amounts contributes to the degradation of the detritus. Similarly, this study proves the ubiquitous presence of meiofauna in macrophytodetritus. Depending on the season, densities from 20.10³ to 160.10³ meiofaunal organisms per square metre of accumulation were recorded. Copepods were the most abundant taxon (> 50%) of which 87% belonged to the order Harpacticoida. Nematodes were the second most abundant taxon, representing on average 18% of the total meiofauna densities. A total of 61 copepod species were found in Calvi Bay macrophytodetritus accumulations and adjacent habitats (bare sand, seagrass and water column), wherefrom 85% were shared amongst these habitats, underlining the high colonization capabilities of copepods. Active colonization occurred within 24h through species-specific dispersal pathways. Certain species were more avid to colonize, resulting in a colonizer-competitor trade-off among the copepod community. Eco-morphological characteristics seemed to be responsible for the dispersal potential. However, the variety of the composition of the copepod community suggested that other factors also contributed to the attractiveness of the structurally complex macrophytodetritus habitat. The isotopic niches of four abundant copepod species, representing four different eco-morphological groups were identified: Ectinosoma dentatum (mesopsammic-type), Diosaccus tenuiremis (phytal-type), Tisbe furcata (epibenthic-type) and Clausocalanus arcuicornis (water-column-type). Based on stable isotope analysis, fatty acid profiling and Bayesian mixing model, results suggested an interspecific diversity which would indicate a species-specific resource partitioning. C. arcuicornis mainly fed on suspended organic matter, while D. tenuiremis thrived mainly on epiphytes (mostly diatoms). E. dentatum was dependent on the seasonal availability of food sources, while T. furcata fed on a heterogeneous mixture of sources. Presumably none of the species directly assimilated dead seagrass leaf litter. Overall, by combining in situ sampling, novel mesocosm experiment, biomarkers and mixing models, this study displayed the carrying capacity of macrophytodetritus to support a large amount of meiofauna and a wide diversity of copepod species. The morphological differences among copepod species seem to allow specialization towards habitat preferences, (physical habitat preferences and colonization potential) and towards resource preferences (food partitioning). Macrophytodetritus seems thus to be a suitable home, or a temporary hub for a diverse copepod community. Finally, this dynamic and patchy habitat, prone to swift changes and situated at the crossing of different ecosystems, plays a major role in coastal ecology. [less ▲]

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See detailThe cultural Other’s ways of eating. On embodied subjectivities, diets and inclusion of diversity.
Mescoli, Elsa ULg

Conference (2015, June 09)

The notion of subjectivation is employed in the human and social sciences to define the dynamic and complex process of definition of the self. In this process, several factors interact, both rising from ... [more ▼]

The notion of subjectivation is employed in the human and social sciences to define the dynamic and complex process of definition of the self. In this process, several factors interact, both rising from those which appear as inner needs and from cultural and social expectations. This occurs at different levels of the individual’s everyday life’s experience, one of which is the material one. At this level we find diets, which actively perform on corporeality meant as embodied self. The materiality of food habits and culinary practices is the site where the stratified definition of individual subjectivity take place and where cultural and social belongings are represented and negotiated. In order to support this statement, I will present the life history of the key informant of my doctoral research that was devoted to the culinary practices of a group of Moroccan women living in context of migration. The ethnographic data were collected during an eighteen months fieldwork conducted in Milan hinterland (Italy). Through participant observation and semi-structured interviews, I studied the way of eating of this woman and her family. Their diet was influenced by several factors, among which the most relevant one was the illness of one of the children, a young girl suffering from diabetes mellitus. The (micro)materiality of food choices made by her mother for her nourishment, played a crucial role in the definition of the girl’s body and of its representation. In fact, this woman negotiated her culinary practices, stemming from her embodied memory, with medical prescriptions and instruments in order to restore her child’s body to health and thus shaping it as socially acceptable. We see a double-direction movement involving food and the body: the incorporation of appropriate nourishment, assured by a strict measurement of food biological components, and the excorporation of some physiological functions delegated to external medical devices. Such dynamics are aimed at making the child’s body comply with those biomedical norms that control the individuals’ access to the “healthy collectivity”. The medical work on the body through food corresponds to the normalization of the cultural Other’s ways of eating, a process that seems to be fundamental to migrants’ integration in the host society. In this paper I will discuss these issues focusing on food practices as material culture which actively participates to the embodied process of subjectivation. I will support my presentation with visual materials. [less ▲]

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See detailA new design of organic radical batteries (ORBs): carbon nanotube buckypaper electrode functionalized by electrografting
Aqil, Abdelhafid ULg; Vlad, Alexandru; Piedboeuf, Marie-Laure ULg et al

in Chemical Communications (2015), 51(45), 9301-9304

A novel hybridmaterial displaying a fast and reversible charge storage capability is prepared by electrografting of an alkoxyamine-bearing acrylate onto a carbon nanotube buckypaper, followed by the quan ... [more ▼]

A novel hybridmaterial displaying a fast and reversible charge storage capability is prepared by electrografting of an alkoxyamine-bearing acrylate onto a carbon nanotube buckypaper, followed by the quan- titative generation of an electroactive polynitroxide. [less ▲]

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See detailDe la victimisation à l’agression sexuelle à l’adolescence : dynamiques des Trans-Missions intra et inter-générationnelles
Glowacz, Fabienne ULg

Conference (2015, June 05)

Parmi les facteurs étiologiques du passage à l’acte sexuel, la clinque et de nombreuses études mettent en exergue l’expérience antérieure de victimisation sexuelle chez les auteurs d’infraction à ... [more ▼]

Parmi les facteurs étiologiques du passage à l’acte sexuel, la clinque et de nombreuses études mettent en exergue l’expérience antérieure de victimisation sexuelle chez les auteurs d’infraction à caractère sexuel (AICS). Même si le taux de victimisation sexuelle chez les adolescents AICS est extrêmement variable d’une étude à l’autre, un vécu de victimisation, sans pour autant être le seul facteur étiologique, pourrait être considéré comme un des facteurs de risque dans une constellation plurifactorielle, et ce dans un contexte affectif et social particulier. La méta-analyse de Paolucci, Genuis et Violato (2001) révélait qu’un antécédent d’AS augmentait seulement de 8% le risque de perpétuer le cycle de victime à agresseur. Plutôt que de se centrer sur le débat portant sur les taux de prévalence existant, il nous a semblé intéressant de comprendre comment s’activent les dynamiques transmissives inter et intra-générationnelles. Le concept de cycle intergénérationnel, englobant toute forme de victimisation vécue dans l’enfance du parent et de l’enfant, a surtout été étudié chez l’enfant qui subit un abus sexuel ; notre exposé réactualisera ce concept dans le contexte de l’agression sexuelle commise par l’adolescent. Nous aborderons le cycle et la transmission de l’abus sexuel, d’une part au travers de la victimisation sexuelle d’un parent de l’adolescent commettant une agression sexuelle, et d’autre part, au travers d’un vécu de victimisation sexuelle par l’adolescent qui prend sens au niveau des agressions qu’il va commettre. C’est à partir de trois cas d’adolescent de 14 ans ayant commis des agressions sexuelles sur des enfants de jeune âge (en intra et/ou extrafamilial) que les dynamiques de transmission vont être décodées à partir d’hypothèses psycho-développementales et systémiques. Une première situation se caractérise par la levée du secret de la victimisation de la mère lors du dévoilement de l’agression sexuelle que son adolescent a commise, qui mène à envisager les dynamiques de trans-missions indirectes et de délégation par la mère, s’appuyant sur son incapacité à se connecter et à verbaliser son vécu d’abus et la rage associée, se révélant comme un des médiateurs du processus de transmission. La deuxième situation concerne un adolescent qui, tout comme sa sœur, a subi des abus sexuels par son père, et qui, à son tour, a commis de multiples abus sexuels sur sa sœur. Les dynamiques de transmission par répétition au travers du processus d’identification à l’agresseur et de légitimité destructrice seront développées. Une troisième situation d’un adolescent ayant subi des abus sexuels par un tiers extrafamilial et agissant à son tour à 14 ans des agressions sexuelles sur tous les enfants de la fratrie, nous permettra d’envisager le contexte psycho-affectif familial et de fratrie comme facilitateur de transmission intra-générationnelle. Ces trois situations illustreront la vulnérabilité de l’adolescent qui a été confronté directement ou indirectement dans son histoire personnelle et familiale à un vécu de victimisation, durant cette étape développementale qu’est l’adolescence caractérisée par des enjeux identitaires, relationnels et sexuels. [less ▲]

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See detailLa dimension adaptative du fonctionnement pervers : une question complexe
Englebert, Jérôme ULg

Conference (2015, June 04)

Après avoir rappelé la différence entre perversion et fonctionnement psychologique pervers, nous proposerons une réflexion éthologique et écologique qui s’appuiera sur une pratique clinique en hôpital ... [more ▼]

Après avoir rappelé la différence entre perversion et fonctionnement psychologique pervers, nous proposerons une réflexion éthologique et écologique qui s’appuiera sur une pratique clinique en hôpital sécuritaire avec plusieurs patients au fonctionnement psychologique pervers. Dans un raisonnement où nous distinguons la signification d’un comportement de sa fonction, il apparaît que la maîtrise du territoire et du rang social sont des « facultés » perverses observables. Nous suggérons de concevoir le pervers comme un individu hyper-adapté à un environnement social (la prison, par exemple, mais pas seulement) dont il maîtrise remarquablement l’art de la relation et de la « territorialisation ». Nous mettrons également en évidence les grandes compétences perceptives de ces patients (notamment via une réponse au test de Rorschach) et la maitrise fulgurante de l’intuition relationnelle. L’ensemble de ces caractéristiques font du pervers un être très « compétent » d’un point de vue social. Ceci explique, lorsqu’ils s’expriment sur le terrain de la délinquance, la dimension « imparable » des actes criminels de ces individus. Au-delà de cette adaptation sociale performante et remarquable, surviennent néanmoins des moments paradoxaux où la « désadaptation » est complète. Ces « moments pervers », qui sont rencontrés dans la clinique avec ces patients et qui apparaissent inexplicables d’un point de vue éthologique et adaptatif, sont des phénomènes essentiels à mettre en évidence dans un processus d’évaluation diagnostique. Dès lors, le pervers doit être considéré comme un être dont le fonctionnement adaptatif est complexe, fluctuant sur un continuum entre hyper-adaptation (la plupart du temps) et inadaptation (s’exprimant sous la forme d’un hiatus). Précisons, en outre, que ce modèle adaptatif suggère clairement que le fonctionnement psychologique pervers n’est pas l’apanage du sujet délinquant sexuel, pas plus qu’il n’est celui du délinquant en règle général. Ce mode de fonctionnement est également à identifier dans de nombreuses situations sociales où l’exercice du pouvoir sur autrui est accepté voire encouragé. Pour conclure, nous insisterons sur le fait qu’un fonctionnement psychologique pervers est complexe à mettre en évidence, notamment parce qu’il demande du temps et le développement de qualités d’observation parfois difficilement opérationnelles dans certaines situations cliniques ou d’expertise. Pour pouvoir améliorer la connaissance de ce fonctionnement, cette entité demande une reconnaissance nosographique et diagnostique propre. Notre hypothèse d’une hyper-adaptation pourrait être éprouvée par des méthodes cognitives. Nous pensons, par exemple, à un dispositif proposant des tâches de résolutions de problèmes sans issue. Cependant, l’on peut se demander s’il est raisonnable d’envisager d’avoir une connaissance nomothétique de la personnalité perverse. Le nombre restreint de sujets « disponibles » pour une évaluation, leur logique insaisissable, leur tendance au refus de collaborer à une étude s’ils n’y trouvent pas avantage, etc. Ces arguments font que le sujet pervers échappe presque par principe aux méthodes de recherche conventionnelles. Cela ne veut évidemment pas dire que l’entité nosographique n’existe pas. Tout en connaissant le lourd tribut que peut représenter le fait d’apposer ce diagnostic à propos d’un sujet, nous estimons, néanmoins, qu’il est important de pouvoir le mettre en évidence lorsqu’il est présent (comme il est, par ailleurs, essentiel de se garder de le poser lorsqu’il est absent). Pour ce faire, la répétition et l’observation sont les seuls moyens de parvenir à objectiver ce type de fonctionnement en réduisant le risque d’identifier des faux positifs. La difficulté essentielle est et restera de parvenir à parler à écrire sur le pervers. Pointer une dynamique perverse et parvenir à la faire comprendre aux autres relève presque de l’impossible car, d’une certaine manière, le pervers est toujours dans le juste. Il n’a pas toujours une bonne excuse, il a toujours une bonne raison. Le principe pervers dépasse le dicible, la dynamique perverse est difficilement verbalisable. [less ▲]

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See detailAdolescentes délinquantes sexuelles : victimisation sexuelle et agression sexuelle, résonnances et dissonances
Glowacz, Fabienne ULg

Conference (2015, June 03)

Peu d’études portant sur les adolescentes auteures d’une infraction à caractère sexuel sont menées à ce jour. De manière générale, la délinquance des filles a suscité un moindre intérêt et surtout a été ... [more ▼]

Peu d’études portant sur les adolescentes auteures d’une infraction à caractère sexuel sont menées à ce jour. De manière générale, la délinquance des filles a suscité un moindre intérêt et surtout a été entachée de mythes, de stéréotypes et de représentations qui ont entravé la levée du voile. Dans le champ de recherche sur l’agression sexuelle, la femme a été prioritairement associée au statut de victime, et il a longtemps été impensable que des adolescentes puissent commettre des violences sexuelles. Envisager la jeune fille comme auteure d’agression sexuelle a nécessité des avancées au niveau de la reconnaissance de la réalité des violences sexuelles perpétrées par les mineurs et de la délinquance sexuelle chez les femmes adultes (Tardif, Auclair, Jacob et Carpentier, 2005). Lane et Lobanov-Rostovsky (1997) estiment que les AAICS de sexe féminin constitueraient entre 5% et 8% de l’ensemble des AAICS. Il apparait donc que les filles sont plus souvent victimes d’abus sexuels que les garçons mais elles semblent moins nombreuses que les garçons à commettre des agressions sexuelles. Par ailleurs, les études en délinquance non sexuelle ont démontré que la victimisation sexuelle durant l’enfance est un facteur significativement associé au risque de conduites déviantes et de trajectoire délinquante durant l’adolescence, particulièrement pour les filles. Au niveau de l’âge adulte, il a été relevé des taux élevés d’antécédents de victimisation sexuelle (Goodkind, Ng et Sarri, 2006) : les femmes en détention présentent deux à trois fois plus d’antécédents de victimisation sexuelle durant leur enfance que les autres. L’abus sexuel apparait clairement pour les filles, plus que pour les garçons, comme un facteur de risque par rapport à l’adoption de conduites délinquantes durant l’adolescence. Des caractéristiques propres à l’abus sexuel peuvent augmenter le risque de délinquance et d’agirs violents, comme la durée et la chronicité de la période d’abus ainsi que le non-dévoilement des violences subies. Dans une de nos études, nous avons relevé que les adolescentes engagées dans la délinquance ayant été victimes d’abus sexuel sont plus nombreuses à présenter un PTSD que les adolescentes victimes non délinquantes, et surtout présentent davantage de symptômes d’évitement (Glowacz et Buzitu, 2014). La délinquance a dès lors été considérée comme une conduite d’évitement visant une mise à distance émotionnelle de la douleur associée à la victimisation sexuelle. Les conduites délinquantes apparaissent comme une forme de stratégie adaptative via une mise à distance du trauma, qui cependant entrave le processus de résilience. Bien que le taux de victimisation sexuelle dans la population des adolescentes délinquantes sexuelles soit variable selon les études, la victimisation est reconnue comme un des facteurs étiologiques de l’agression sexuelle. Wijkman, Bijleveld et Hendriks (2014) l’évaluent à 37% dans leur échantillon de jeunes délinquantes sexuelles alors que d’autres études arrivent à des taux plus élevés. Dans le cadre de la présente étude portant sur l’analyse de dossiers psycho-judiciaires, nous présenterons d’abord les profils des adolescentes délinquantes sexuelles et une ébauche de typologie. Nous nous intéresserons ensuite aux adolescentes AICS pour lesquelles il est signalé une victimisation sexuelle afin d’en identifier les spécificités mais surtout les résonnances et les dissonances au niveau de l’abus sexuel subi et de l’abus sexuel commis. Les facteurs sociaux, familiaux, psycho-développementaux seront analysés à la lumière des dynamiques de transmission de l’abus sexuel subi par la perpétration d’agressions sexuelles. [less ▲]

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See detailNovel nanosized water soluble fluorescent micelles with embedded perylene diimide fluorophores for potential biomedical applications: Cell permeability, localization and cytotoxicity
Bryaskova, Rayna; Georgiev, Nikolai I.; Dimov, Stefan M. et al

in Materials Science & Engineering : C (2015), 51

Novel biocompatiblewater-soluble fluorescent micelleswithembedded perylene diimides (PDI) for intracellular applications have been prepared by self assembling of amphiphilic poly(vinyl alcohol)-b-poly ... [more ▼]

Novel biocompatiblewater-soluble fluorescent micelleswithembedded perylene diimides (PDI) for intracellular applications have been prepared by self assembling of amphiphilic poly(vinyl alcohol)-b-poly(acrylonitrile) (PVA-b-PAN) copolymers in the presence of synthesized fluorophores. Amphiphilic PVA-b-PAN copolymers were obtained by selective hydrolysis of well-defined poly(vinyl acetate)-b-poly(acrylonitrile) (PVAc-b-PAN) copolymer. The preparation of the novel fluorescence micelles consisting of PVA hydrophilic shell and PAN hy- drophobic core with incorporated PDI fluorophores has been confirmed by DLS and TEManalysis. The cytotoxic- ity of the water-soluble fluorophores and their internalization into living cells depending on the micellar concentration have been tested. Itwas shown that they could successfully enter in living cellswithout destroying their morphology. The results obtained indicate that the novel water-soluble fluorescent micelles with embed- ded PDI fluorophores would be suitable for potential intracellular biomedical applications. [less ▲]

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See detailTriiodothyronine-predominant Graves’ disease in childhood: detection and therapeutic implications
HARVENGT, Julie ULg; boizeau, priscilla; chevenne, didier et al

in European Journal of Endocrinology (2015), 172(6), 715-723

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See detailMacroscopic analysis of interaction models for the provision of flexibility in distribution systems
Mathieu, Sébastien ULg; Ernst, Damien ULg; Cornélusse, Bertrand ULg

in Proceedings of the International Conference on Electricity Distribution, CIRED 2015 (2015, June)

To ease the transition towards the future of distribution grid management, regulators must revise the current interaction model, that is, the set of rules guiding the interactions between all the parties ... [more ▼]

To ease the transition towards the future of distribution grid management, regulators must revise the current interaction model, that is, the set of rules guiding the interactions between all the parties of the system. Five interaction models are proposed, three of them considering active network management. This paper evaluates the economic efficiency of each model using macroscopic representation of the system, by opposition to more techniques requiring a complete picture of the system. The interaction models are simulated on the horizon 2015-2030. Results show that for the first five years all the models provide similar economic efficiency. For the remaining ten years, interaction models implementing active network management provide up to a 10% higher economic efficiency. [less ▲]

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See detailMeasurement of 3D Room Impulse Responses with a Spherical Microphone Array
Embrechts, Jean-Jacques ULg

in Proceedings of the Euronoise 2015 Congress (2015, June)

Directional room impulse responses (DRIRs) are composed of the sound contributions reaching a given location in the room from a well-defined direction in space. DRIRs can be useful in many applications ... [more ▼]

Directional room impulse responses (DRIRs) are composed of the sound contributions reaching a given location in the room from a well-defined direction in space. DRIRs can be useful in many applications, such as the evaluation of spatial room acoustics parameters, the detection of unwanted specular reflections or the 3D auralization of acoustic spaces. A spherical array containing 16 microphones has been realized to measure DRIRs. The logarithmic sinesweep technique is first applied to measure 16 impulse responses, one for each microphone. A spherical harmonics (SH) decomposition of the sound field is then obtained. Spatial aliasing, placement errors and the ‘white noise gain’ (WNG) have been analysed to define the useful bandwith of this measure, i.e. [250Hz – 4kHz]. The coefficients of the SH decomposition are then processed by some beamforming methods, in order to compute the DRIR in any direction around the spherical array. Time and 3D space representations can be generated. The results obtained in some rooms are illustrated in this paper: it is shown that the combination of the ‘delay-and-sum’ and ‘minimum-variance distorsionless response’ beamforming methods is particularly well suited for the analysis of DRIRs. [less ▲]

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See detailDescription of the research project CIMEDE for the industrial construction of evolutionary, sustainable and economic houses
Duthoit, Fabienne ULg; Embrechts, Jean-Jacques ULg

in Proceedings of the Euronoise 2015 Congress (2015, June)

CIMEDE is a research project supported by the Region of Wallonia (Belgium). It stands for CIMEDE “Construction Industrielle de Maisons Evolutives, Durables et Economiques – industrial construction of ... [more ▼]

CIMEDE is a research project supported by the Region of Wallonia (Belgium). It stands for CIMEDE “Construction Industrielle de Maisons Evolutives, Durables et Economiques – industrial construction of evolutionary, sustainable and economic houses”. Since the materials envisaged for this project are timber and gypsum fibre board, it was necessary to design a constructive principle of lightweight timber frame constructions which meets the industrial, sustainable, evolutionary and economic requirements and also the requirements of the Belgian acoustic standard NBN S01-400-1. First of all, an inventory of constructive elements that are currently used in this type of construction (floors, walls and facades) is presented with their acoustic performances. Then, the laboratory measurements of some samples of floors, facades, interior walls and partition walls are described and discussed. On the basis of the measurement results, several solutions that best meet all criteria were retained and a mock-up was built with these constructive elements. Measurement results on this mock-up are also presented. The final solution for the floor, the partition wall, the interior wall and the facade which meets the acoustic requirements is finally described [less ▲]

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See detailOccupational physicians managing workers long-term sickness absence : are they willing to cooperate with family and social insurance physicians ?
Mairiaux, Philippe ULg; Vanmeerbeek, Marc ULg; Schippers, Nathalie ULg et al

Conference (2015, June)

Introduction In Belgium, sickness absence (SA) management implies complementary roles for family (FPs), social insurance (SIPs) and occupational physicians (OPs): FPs deliver sick notes, SIPs control SA ... [more ▼]

Introduction In Belgium, sickness absence (SA) management implies complementary roles for family (FPs), social insurance (SIPs) and occupational physicians (OPs): FPs deliver sick notes, SIPs control SA benefits and OPs strive to adapt work environments to workers’ capacities. In practice however, there is little cooperation between the three physicians. This study aimed at identifying FP-OP-SIP cooperation channels in order to prevent long-term work disability. Methods The study involved two phases. (1) Using the nominal group technique, researchers from the three physician groups reached a consensus on 15 proposals to enhance inter-professional cooperation. (2) A Delphi study was conducted to validate these proposals: 61 experts representing professional groups of physicians (FP, SIP, OP), patients, government, employers and labor unions were asked to participate. A 18-items questionnaire (the 15 proposals + 3 open questions) was used in a 2-round Delphi study. Proposals were accepted if 80% of experts agreed. Results Participation rate was 77% in round 1 and 7 out of the 15 proposals were accepted. During round 2, participation rate was 94%; 2 of the remaining proposals reached agreement level. Public authorities were thus advised to invest in a) promotion of pre-return to work visit with the OP for sick-listed patients; b) a website with OPs contact data; c) joint guidelines for return to work guidance; d) joint training of the 3 physician groups; 5) safe electronic information exchange. Several proposals aiming at giving work-related information to FPs and SIPs did not reach agreement within the OPs expert group. OPs expressed concerns about their own workload and FPs or SIPs ability to understand and use those information. Discussion The need for inter-physician cooperation in disability management may exceed individual goodwill and should best be organised by public authorities. This study may be a first step in this process. [less ▲]

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